Click here to switch to the map view.

The map label for this gene is cpdB [H]

Identifier: 29144701

GI number: 29144701

Start: 4600835

End: 4602778

Strand: Reverse

Name: cpdB [H]

Synonym: t4456

Alternate gene names: 29144701

Gene position: 4602778-4600835 (Counterclockwise)

Preceding gene: 29144703

Following gene: 29144699

Centisome position: 96.05

GC content: 51.8

Gene sequence:

>1944_bases
ATGATTAAGTTTAGTGCAACGCTTCTGGCTACGCTGATCGCCGCCAGCGTGAATGCGGCCACCGTCGATCTCCGTATCAT
GGAAACTACTGATTTACATAGCAACATGATGGATTTCGATTATTACAAGGATACCGCAACCGAAAAATTCGGATTAGTAC
GTACCGCAAGTTTGATTCATGCCGCCCGAAATGAAGTCAAAAACAGCGTGCTGGTGGATAACGGCGACCTGATTCAGGGG
AGCCCTCTGGGCGATTATATGGCGGCAAAAGGGCTCAAAGACGGTGATGTTCACCCGGTCTATAAAGCGCTCAATACTCT
GGATTACGCGGTTGGAAACCTCGGCAACCATGAGTTTAATTATGGTCTGGACTATCTGCATAACGCGCTGGCCGGCGCGA
AATTCCCTTATGTTAACGCCAATATTATCGACGTTAAAACCCAAAAGCCGCTCTTTACGCCTTATCTGATTAAAGAGACG
AGCGTTATTGATAAAGACGGCCACCCGCACACTCTGAAAATCGGCTATATCGGCTTTGTTCCGCCGCAAATCATGATTTG
GGATAAAGCGAATCTGAGCGGCAAAGTTACCGTCAATGACATTACGGAAACCGCCCGGAAATATGTGCCCGAAATGCGCG
AGAAAGGCGCTGATATCGTGGTAGTCATTGCTCACTCAGGCCTTTCTGCCGATCCGTACCACAGCATGGCGGAAAACTCG
GTTTATTACCTCAGCGAAGTTCCCGGCGTCGACGCGATTATGTTTGGTCACGCCCACGCCGTCTTCCCCGGTAAAGATTT
TGCCGACATCAAAGGCGCGGATATCGCCAAATGTACGCTTAACGGCATTCCTGCGGTAATGCCAGGCATGTGGGGCGACC
ATCTGGGAGTGGTCGATCTGGTGCTCAATAACGACAGCGGCAAATGGCAGGTTACGCAGGCGAAAGCGGAAGCGCGGCCT
ATTTACGACGCCGCCGCTAAAAAGTCGCTGGCGGCGGAAGACAGTAAGCTGGTCGGTATTCTGAAAGCCGATCACGATGC
CACCCGCGAGTTTGTCAGCAAGCCCATCGGTAAATCCGCCGATAATATGTACAGTTATCTTGCTCTGGTGCAGGACGATC
CTACCGTACAGGTGGTGAATAACGCGCAAAAAGCGTATGTGGAACACTTTATTCAGGGCGATCCGGATCTGGCGAAACTG
CCGGTGCTGTCCGCCGCCGCGCCGTTTAAAGTTGGCGGGCGTAAGAACGATCCTGCCAGCTTTGTCGAGGTGGAAAAAGG
CCAGCTGACCTTCCGTAACGCCGCGGATCTTTATCTCTATCCGAATACGCTGGTGGTGGTGAAAGCCAGCGGCAAGGAGG
TAAAAGAGTGGCTGGAATGTTCTGCCGGACAGTTTAATCAGATTGATATTCACAGCAACAAACCGCAATCGTTGATCAAT
TGGGATGGCTTTCGCACCTATAATTTCGATGTCATTGACGGTGTGAATTATCAAATCGATGTGTCACAGCCCGCCCGCTA
TGATGGCGAATGCCAGATGGTGAATCCGCAGGCGGAACGTATTAAAAACCTGACCTTTAACGGTAAGCCTGTCGACCCGA
ACGCTACGTTCCTGGTCGCCACGAATAACTATCGCGCTTACGGCGGCAAGTTTGCCGGCACGGGCGACAGCCATATCGCC
TTTGCGTCTCCGGATGAAAATCGCGCCGTGCTGGCGGCATGGATTGGCGCAGAGTCAAAACGCGCGGGCGAAATCCATCC
GGCGGCGGATAATAACTGGCGTCTGGCGCCAATCCACAGCGATACGGCGCTGGATATCCGCTTTGAAACATCGCCAGGCG
ATAAAGCCGCGGCCTTCATTAAAGAGAAAGGGCAGTATCCGATGAAAAAGGTTGGTGTCGATGATATCGGTTTTGCCATT
TATCAGGTGGATTTGAGCAAGTAA

Upstream 100 bases:

>100_bases
CCTTCTTCTGGCGACGGTCGGAACGATACCGGTTTCACTGTTTATCATCGCTATTTTTCACTATCCGGCTTTTCTATTGC
GTTCTTAAAAGGTGCCTCTG

Downstream 100 bases:

>100_bases
CGTGAGTCCCGTATGGCGCTGCGTTTATTCGGCCTGACTGGCAGGCCGAATAAAGCGCTAGCCGCCATCTGATAAACGCT
CCCGCTGCGCCAATACCTGG

Product: bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKDTATEKFGLVRTASLIHAARNEVKNSVLVDNGDLIQG
SPLGDYMAAKGLKDGDVHPVYKALNTLDYAVGNLGNHEFNYGLDYLHNALAGAKFPYVNANIIDVKTQKPLFTPYLIKET
SVIDKDGHPHTLKIGYIGFVPPQIMIWDKANLSGKVTVNDITETARKYVPEMREKGADIVVVIAHSGLSADPYHSMAENS
VYYLSEVPGVDAIMFGHAHAVFPGKDFADIKGADIAKCTLNGIPAVMPGMWGDHLGVVDLVLNNDSGKWQVTQAKAEARP
IYDAAAKKSLAAEDSKLVGILKADHDATREFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHFIQGDPDLAKL
PVLSAAAPFKVGGRKNDPASFVEVEKGQLTFRNAADLYLYPNTLVVVKASGKEVKEWLECSAGQFNQIDIHSNKPQSLIN
WDGFRTYNFDVIDGVNYQIDVSQPARYDGECQMVNPQAERIKNLTFNGKPVDPNATFLVATNNYRAYGGKFAGTGDSHIA
FASPDENRAVLAAWIGAESKRAGEIHPAADNNWRLAPIHSDTALDIRFETSPGDKAAAFIKEKGQYPMKKVGVDDIGFAI
YQVDLSK

Sequences:

>Translated_647_residues
MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKDTATEKFGLVRTASLIHAARNEVKNSVLVDNGDLIQG
SPLGDYMAAKGLKDGDVHPVYKALNTLDYAVGNLGNHEFNYGLDYLHNALAGAKFPYVNANIIDVKTQKPLFTPYLIKET
SVIDKDGHPHTLKIGYIGFVPPQIMIWDKANLSGKVTVNDITETARKYVPEMREKGADIVVVIAHSGLSADPYHSMAENS
VYYLSEVPGVDAIMFGHAHAVFPGKDFADIKGADIAKCTLNGIPAVMPGMWGDHLGVVDLVLNNDSGKWQVTQAKAEARP
IYDAAAKKSLAAEDSKLVGILKADHDATREFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHFIQGDPDLAKL
PVLSAAAPFKVGGRKNDPASFVEVEKGQLTFRNAADLYLYPNTLVVVKASGKEVKEWLECSAGQFNQIDIHSNKPQSLIN
WDGFRTYNFDVIDGVNYQIDVSQPARYDGECQMVNPQAERIKNLTFNGKPVDPNATFLVATNNYRAYGGKFAGTGDSHIA
FASPDENRAVLAAWIGAESKRAGEIHPAADNNWRLAPIHSDTALDIRFETSPGDKAAAFIKEKGQYPMKKVGVDDIGFAI
YQVDLSK
>Mature_647_residues
MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKDTATEKFGLVRTASLIHAARNEVKNSVLVDNGDLIQG
SPLGDYMAAKGLKDGDVHPVYKALNTLDYAVGNLGNHEFNYGLDYLHNALAGAKFPYVNANIIDVKTQKPLFTPYLIKET
SVIDKDGHPHTLKIGYIGFVPPQIMIWDKANLSGKVTVNDITETARKYVPEMREKGADIVVVIAHSGLSADPYHSMAENS
VYYLSEVPGVDAIMFGHAHAVFPGKDFADIKGADIAKCTLNGIPAVMPGMWGDHLGVVDLVLNNDSGKWQVTQAKAEARP
IYDAAAKKSLAAEDSKLVGILKADHDATREFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHFIQGDPDLAKL
PVLSAAAPFKVGGRKNDPASFVEVEKGQLTFRNAADLYLYPNTLVVVKASGKEVKEWLECSAGQFNQIDIHSNKPQSLIN
WDGFRTYNFDVIDGVNYQIDVSQPARYDGECQMVNPQAERIKNLTFNGKPVDPNATFLVATNNYRAYGGKFAGTGDSHIA
FASPDENRAVLAAWIGAESKRAGEIHPAADNNWRLAPIHSDTALDIRFETSPGDKAAAFIKEKGQYPMKKVGVDDIGFAI
YQVDLSK

Specific function: This bifunctional enzyme catalyzes two consecutive reactions during ribonucleic acid degradation. Converts a 2',3'- cyclic nucleotide to a 3'-nucleotide and then the 3'-nucleotide to the corresponding nucleoside and phosphate [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=647, Percent_Identity=90.2627511591963, Blast_Score=1213, Evalue=0.0,
Organism=Escherichia coli, GI1786687, Length=569, Percent_Identity=22.6713532513181, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR006294
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16 [H]

Molecular weight: Translated: 70640; Mature: 70640

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKDTATEKFGLVRTASLIH
CEEEHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHH
AARNEVKNSVLVDNGDLIQGSPLGDYMAAKGLKDGDVHPVYKALNTLDYAVGNLGNHEFN
HHHHHHCCCEEECCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
YGLDYLHNALAGAKFPYVNANIIDVKTQKPLFTPYLIKETSVIDKDGHPHTLKIGYIGFV
CCHHHHHHHHHCCCCCEECCEEEEEECCCCCCCCEEEECCEEECCCCCCCEEEEEEEEEC
PPQIMIWDKANLSGKVTVNDITETARKYVPEMREKGADIVVVIAHSGLSADPYHSMAENS
CCEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCC
VYYLSEVPGVDAIMFGHAHAVFPGKDFADIKGADIAKCTLNGIPAVMPGMWGDHLGVVDL
EEEEECCCCCEEEEECCEEEECCCCCCCCCCCCCEEEEEECCCCHHCCCCCCCCCCEEEE
VLNNDSGKWQVTQAKAEARPIYDAAAKKSLAAEDSKLVGILKADHDATREFVSKPIGKSA
EEECCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCCCCH
DNMYSYLALVQDDPTVQVVNNAQKAYVEHFIQGDPDLAKLPVLSAAAPFKVGGRKNDPAS
HHHEEEEEEECCCCCEEEECCHHHHHHHHHHCCCCCHHHCCEECCCCCEECCCCCCCCCC
FVEVEKGQLTFRNAADLYLYPNTLVVVKASGKEVKEWLECSAGQFNQIDIHSNKPQSLIN
EEEEECCEEEEECCCEEEECCCEEEEEECCCHHHHHHHHCCCCCCEEEEECCCCCCCEEC
WDGFRTYNFDVIDGVNYQIDVSQPARYDGECQMVNPQAERIKNLTFNGKPVDPNATFLVA
CCCEEEEEEEEECCCCEEEECCCCCCCCCEEEEECHHHHHHHCCCCCCEECCCCCEEEEE
TNNYRAYGGKFAGTGDSHIAFASPDENRAVLAAWIGAESKRAGEIHPAADNNWRLAPIHS
ECCEEECCCEECCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECC
DTALDIRFETSPGDKAAAFIKEKGQYPMKKVGVDDIGFAIYQVDLSK
CCEEEEEEECCCCCHHHHHHHHCCCCCHHHCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKDTATEKFGLVRTASLIH
CEEEHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHH
AARNEVKNSVLVDNGDLIQGSPLGDYMAAKGLKDGDVHPVYKALNTLDYAVGNLGNHEFN
HHHHHHCCCEEECCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
YGLDYLHNALAGAKFPYVNANIIDVKTQKPLFTPYLIKETSVIDKDGHPHTLKIGYIGFV
CCHHHHHHHHHCCCCCEECCEEEEEECCCCCCCCEEEECCEEECCCCCCCEEEEEEEEEC
PPQIMIWDKANLSGKVTVNDITETARKYVPEMREKGADIVVVIAHSGLSADPYHSMAENS
CCEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCC
VYYLSEVPGVDAIMFGHAHAVFPGKDFADIKGADIAKCTLNGIPAVMPGMWGDHLGVVDL
EEEEECCCCCEEEEECCEEEECCCCCCCCCCCCCEEEEEECCCCHHCCCCCCCCCCEEEE
VLNNDSGKWQVTQAKAEARPIYDAAAKKSLAAEDSKLVGILKADHDATREFVSKPIGKSA
EEECCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCCCCH
DNMYSYLALVQDDPTVQVVNNAQKAYVEHFIQGDPDLAKLPVLSAAAPFKVGGRKNDPAS
HHHEEEEEEECCCCCEEEECCHHHHHHHHHHCCCCCHHHCCEECCCCCEECCCCCCCCCC
FVEVEKGQLTFRNAADLYLYPNTLVVVKASGKEVKEWLECSAGQFNQIDIHSNKPQSLIN
EEEEECCEEEEECCCEEEECCCEEEEEECCCHHHHHHHHCCCCCCEEEEECCCCCCCEEC
WDGFRTYNFDVIDGVNYQIDVSQPARYDGECQMVNPQAERIKNLTFNGKPVDPNATFLVA
CCCEEEEEEEEECCCCEEEECCCCCCCCCEEEEECHHHHHHHCCCCCCEECCCCCEEEEE
TNNYRAYGGKFAGTGDSHIAFASPDENRAVLAAWIGAESKRAGEIHPAADNNWRLAPIHS
ECCEEECCCEECCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECC
DTALDIRFETSPGDKAAAFIKEKGQYPMKKVGVDDIGFAIYQVDLSK
CCEEEEEEECCCCCHHHHHHHHCCCCCHHHCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3005231; 7610040; 9278503; 2172762; 9298646 [H]