Click here to switch to the map view.

The map label for this gene is sgaA [H]

Identifier: 29144681

GI number: 29144681

Start: 4585406

End: 4585870

Strand: Direct

Name: sgaA [H]

Synonym: t4436

Alternate gene names: 29144681

Gene position: 4585406-4585870 (Clockwise)

Preceding gene: 29144680

Following gene: 29144682

Centisome position: 95.69

GC content: 53.98

Gene sequence:

>465_bases
ATGAAATTACGTGATTCGCTGGCGGAGAATAATTCTATCCGCTTGCAGGCTGAAGCTAATACCTGGCAGGAAGCCGTAAA
AATTGGTGTGGATTTGCTGGTGGCGGCAGACGTTGTCGAGCCGCGTTACTACCAGGCGATTCTCGATGGCGTGGAACAGT
TCGGCCCTTATTTCGTTATCGCTCCGGGGCTGGTAATGCCGCACGGGCGTCCGGAAGAGGGCGTGAAGAAAACCGGTTTC
TCACTGGTGACGCTGAAAAAGCCGCTGGAGTTCAACCACGAAGATAACGACCCGGTCGATATTCTGATCACTATGGCGGC
GGTTGATGCCAACACTCACCAGGAAGTGGGCATCATGCAGATCGTCAACCTGTTTGAAGACGAAGCCAATTTTGACCGTT
TACGCGCCTGCCGTACCGCGCAGGAAGTCCTGGATCTCATTGACCGCACCAACGCGGCAGCTTAA

Upstream 100 bases:

>100_bases
GGTTGGCGTGCGCAACATGCTATCGCCCGCAGACTTCGGCCCGAAATTGCTGGAAGTGATCAAAGCGCATTTTCCACAGG
ACGTGAAGTAAGGACGCAAC

Downstream 100 bases:

>100_bases
GAAGGAATTGAACATGTCATTACCGATGTTGCAAGTCGCGCTGGACAACCAGACTATGGATAGCGCCTACGAAACCACTC
GCCTGATTGCCGAAGAAGTC

Product: PTS system L-ascorbate-specific transporter subunit IIA

Products: protein histidine; sugar phosphate

Alternate protein names: PTS system ascorbate-specific EIIA component [H]

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MKLRDSLAENNSIRLQAEANTWQEAVKIGVDLLVAADVVEPRYYQAILDGVEQFGPYFVIAPGLVMPHGRPEEGVKKTGF
SLVTLKKPLEFNHEDNDPVDILITMAAVDANTHQEVGIMQIVNLFEDEANFDRLRACRTAQEVLDLIDRTNAAA

Sequences:

>Translated_154_residues
MKLRDSLAENNSIRLQAEANTWQEAVKIGVDLLVAADVVEPRYYQAILDGVEQFGPYFVIAPGLVMPHGRPEEGVKKTGF
SLVTLKKPLEFNHEDNDPVDILITMAAVDANTHQEVGIMQIVNLFEDEANFDRLRACRTAQEVLDLIDRTNAAA
>Mature_154_residues
MKLRDSLAENNSIRLQAEANTWQEAVKIGVDLLVAADVVEPRYYQAILDGVEQFGPYFVIAPGLVMPHGRPEEGVKKTGF
SLVTLKKPLEFNHEDNDPVDILITMAAVDANTHQEVGIMQIVNLFEDEANFDRLRACRTAQEVLDLIDRTNAAA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI2367359, Length=154, Percent_Identity=96.1038961038961, Blast_Score=305, Evalue=8e-85,
Organism=Escherichia coli, GI1790755, Length=101, Percent_Identity=39.6039603960396, Blast_Score=96, Evalue=9e-22,
Organism=Escherichia coli, GI1789302, Length=120, Percent_Identity=39.1666666666667, Blast_Score=89, Evalue=1e-19,
Organism=Escherichia coli, GI1790027, Length=161, Percent_Identity=31.6770186335404, Blast_Score=61, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 17135; Mature: 17135

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS51094 PTS_EIIA_TYPE_2 ; PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRDSLAENNSIRLQAEANTWQEAVKIGVDLLVAADVVEPRYYQAILDGVEQFGPYFVI
CCCCHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEE
APGLVMPHGRPEEGVKKTGFSLVTLKKPLEFNHEDNDPVDILITMAAVDANTHQEVGIMQ
CCCEECCCCCCCCHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHH
IVNLFEDEANFDRLRACRTAQEVLDLIDRTNAAA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKLRDSLAENNSIRLQAEANTWQEAVKIGVDLLVAADVVEPRYYQAILDGVEQFGPYFVI
CCCCHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEE
APGLVMPHGRPEEGVKKTGFSLVTLKKPLEFNHEDNDPVDILITMAAVDANTHQEVGIMQ
CCCEECCCCCCCCHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHH
IVNLFEDEANFDRLRACRTAQEVLDLIDRTNAAA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]