Click here to switch to the map view.

The map label for this gene is sopE [H]

Identifier: 29144557

GI number: 29144557

Start: 4465393

End: 4466115

Strand: Direct

Name: sopE [H]

Synonym: t4303

Alternate gene names: 29144557

Gene position: 4465393-4466115 (Clockwise)

Preceding gene: 29144548

Following gene: 29144574

Centisome position: 93.19

GC content: 39.97

Gene sequence:

>723_bases
GTGACAAAAATAACTTTATCTCCCCAGAATTTTAGAATCCAAAAACAGGAAACCACACTACTAAAAGAAAAATCAACCGA
GAAAAATTCTTTAGCAAAAAGTATTCTCGCAGTAAAAAATCACTTCATCGAATTAAGGTCAAAATTATCGGAACGTTTTA
TTTCGCATAAGAACACTGAGTCTTCTGCAACACACTTTCACCGAGGAAGCGCATCTGAGGGCCGGGCAGTGTTGACAAAT
AAAGTCGTTAAAGATTTTATGCTTCAAACGCTCAATGATATAGATATTAGAGGTAGTGCGAGTAAAGACCCCGCATACGC
CAGCCAGACCCGTGAAGCTATACTATCGGCAGTTTACAGCAAGAATAAAGATCAGTGTTGTAATTTGCTCATCAGCAAAG
GGATCAACATAGCGCCTTTTCTTCAGGAAATTGGCGAAGCAGCGAAAAATGCAGGTCTGCCCGGAACAACCAAAAATGAC
GTTTTTACGCCAAGCGGCGCAGGGGCCAATCCTTTTATAACTCCGTTGATTTCATCAGCAAACAGTAAGTATCCACGTAT
GTTTATCAATCAACATCAGCAGGCATCCTTTAAAATCTATGCGGAGAAGATCATTATGACAGAAGTTGCACCACTGTTTA
ATGAGTGTGCTATGCCGACTCCACAGCAATTCCAACTGATACTAGAAAACATTGCTAATAAATATATACAATACACTCCC
TGA

Upstream 100 bases:

>100_bases
AATTCATCAATCAGATGGACATAGCATTTGCTATAAAAAATAAAAGTATTCCTGCTATCTATATATAAATGAGTTATGTA
CATATAAAAGGATCATTACC

Downstream 100 bases:

>100_bases
ACACAGAAAAACCAAAAAATATGCGGAGCCTCTTCCTGATTAATATGAACCAATAGTATCCATAATTTTCCCCAGGAACT
AACTCCGGAGCTAAACCGTC

Product: invasion-associated secreted protein

Products: NA

Alternate protein names: Effector protein sopE; Toxin sopE [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MTKITLSPQNFRIQKQETTLLKEKSTEKNSLAKSILAVKNHFIELRSKLSERFISHKNTESSATHFHRGSASEGRAVLTN
KVVKDFMLQTLNDIDIRGSASKDPAYASQTREAILSAVYSKNKDQCCNLLISKGINIAPFLQEIGEAAKNAGLPGTTKND
VFTPSGAGANPFITPLISSANSKYPRMFINQHQQASFKIYAEKIIMTEVAPLFNECAMPTPQQFQLILENIANKYIQYTP

Sequences:

>Translated_240_residues
MTKITLSPQNFRIQKQETTLLKEKSTEKNSLAKSILAVKNHFIELRSKLSERFISHKNTESSATHFHRGSASEGRAVLTN
KVVKDFMLQTLNDIDIRGSASKDPAYASQTREAILSAVYSKNKDQCCNLLISKGINIAPFLQEIGEAAKNAGLPGTTKND
VFTPSGAGANPFITPLISSANSKYPRMFINQHQQASFKIYAEKIIMTEVAPLFNECAMPTPQQFQLILENIANKYIQYTP
>Mature_239_residues
TKITLSPQNFRIQKQETTLLKEKSTEKNSLAKSILAVKNHFIELRSKLSERFISHKNTESSATHFHRGSASEGRAVLTNK
VVKDFMLQTLNDIDIRGSASKDPAYASQTREAILSAVYSKNKDQCCNLLISKGINIAPFLQEIGEAAKNAGLPGTTKNDV
FTPSGAGANPFITPLISSANSKYPRMFINQHQQASFKIYAEKIIMTEVAPLFNECAMPTPQQFQLILENIANKYIQYTP

Specific function: Activator for both CDC42 and RAC1 by directly engaging these Rho GTPases and acting as potent guanine nucleotide exchange factor (GEF). This activation results in actin cytoskeleton rearrangements and stimulates membrane ruffling, promoting bacterial entr

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Note=Secreted via the type III secretion system 1 (SPI-1 TTSS) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GEF (guanine exchange factor) sopE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005414
- InterPro:   IPR016019
- InterPro:   IPR016018 [H]

Pfam domain/function: PF05364 SecIII_SopE_N; PF07487 SopE_GEF [H]

EC number: NA

Molecular weight: Translated: 26685; Mature: 26554

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKITLSPQNFRIQKQETTLLKEKSTEKNSLAKSILAVKNHFIELRSKLSERFISHKNTE
CCEEECCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SSATHFHRGSASEGRAVLTNKVVKDFMLQTLNDIDIRGSASKDPAYASQTREAILSAVYS
CHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
KNKDQCCNLLISKGINIAPFLQEIGEAAKNAGLPGTTKNDVFTPSGAGANPFITPLISSA
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCC
NSKYPRMFINQHQQASFKIYAEKIIMTEVAPLFNECAMPTPQQFQLILENIANKYIQYTP
CCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCC
>Mature Secondary Structure 
TKITLSPQNFRIQKQETTLLKEKSTEKNSLAKSILAVKNHFIELRSKLSERFISHKNTE
CEEECCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SSATHFHRGSASEGRAVLTNKVVKDFMLQTLNDIDIRGSASKDPAYASQTREAILSAVYS
CHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
KNKDQCCNLLISKGINIAPFLQEIGEAAKNAGLPGTTKNDVFTPSGAGANPFITPLISSA
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCC
NSKYPRMFINQHQQASFKIYAEKIIMTEVAPLFNECAMPTPQQFQLILENIANKYIQYTP
CCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA