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The map label for this gene is dnaB [H]

Identifier: 29144487

GI number: 29144487

Start: 4395333

End: 4396697

Strand: Direct

Name: dnaB [H]

Synonym: t4227

Alternate gene names: 29144487

Gene position: 4395333-4396697 (Clockwise)

Preceding gene: 29144486

Following gene: 29144488

Centisome position: 91.72

GC content: 53.04

Gene sequence:

>1365_bases
ATGAGCCATAAATCTGTTTCTGCAGTATCCATGCTGTTTAATGTCGGAGCTGAAACCGGTGTTCTGGGCGGACTGATGCT
CGATAACGACCGCTGGGATGAAATTGCGCCGCTTCTGAATTCCGGAGATTTTTATTATGGCCAGCATCAGATCATTTTTC
GGGAGATAGAACGGTTGGTCAGTGCTGGCCTGCCCATTGACCTGATAACCCTGTCCGAATCCATGGAGCGAAAAGATCTG
CTGGAGCGGTGCGGGGGGGTCGCTTATCTGGCTGAACTGTCAAAAAACACGCCCAGCGCCGCCAATATCGTTGCTTATGC
CGAAATTGTGCGCGAGTACAGTCGTAAGCGACGGCTGGTTAAGCTGGGGCATGAGCTACATCAACTGGCCTCAGTCGGCG
ATGCGGATATACCCGGTCTGATTGAAAAGGCCGAAAAGCAGTTATTTAGTCTGGCTCAGCAGGCCTGTGACCCCGACATT
TGTCTGAGCGTAACGACTCAGGTCGCTGACACGGTCAGCTGGCTGGAATCGGTCAGTGGGGGAAGCGGTGTGACCGGTAC
AGCGACAGGTTTTGCTGAACTGGATGAGAAAACCTGTGGCTGGCAGGACGGTGACCTGATTTTACTGGGTGCCCGGCCAT
CAATGGGGAAAACGGCAGAAGCACTGAAACATGCCGTTGCTGCGCTGGAAGGCAGTCCCGGCAAGACCGTTCAGTTCTAC
AGCATAGAGATGCCAACCCAGCAGCTGATAATGCGTCTGCTTTCAATGCTGGCCAGAGTGCCCTTCGATAACCTGCGCAA
AGGTCTGCTGAGCGAAGGGCAGTGGAGTTTACTTGCGGATGCCGTAGCAAAACTGACCTCCTGGGAAGGACGTTTGCTGA
TAGACGATACCAGTTATCAGACGCCTTCCACGTTACGTATCAGCGCGCGGCGTAATGTGCGTAAATACGGTCAGCCCTCA
CTTATTCTTGTTGATTATCTTCAGTTAATGAGTTGTCCTGGCCGGGAGAACCGCACTCAGGAAATTCAGGAGATTTCCCG
TTCGCTCAAGTCACTGGCCAAAGAGATAAGGTGCCCTGTGGCAGCTTTATCCCAGCTCAATCGCAGCGTTGAACAGCGTC
AGGATAAACGTCCGATGGTCAGTGACCTTCGGGATTCCGGCTCACTGGAGCAGGATGCGGATGTCATCATGTTCCTGTAC
CGCGATGAAGTTTATAACGAGCACTCCCCGGATAGAGGCATAGCTGAAATCATTCTGGGTAAGCAACGTCAGGGGCCGCT
GGGCACTGTCAAAGTTCGCTTTGAGGGGGAGTATATGCGCTTCTCGGAGTATCACCTTGGTTATGGGGAGGTGAGCTATG
GCTAA

Upstream 100 bases:

>100_bases
ACCACCTGGTCTGTGAGTTATTTCCTCAATGGAAAGATAAGTTTGATGCGGTTCTGGTTAACCGGCCTCAGCCCGGATTT
GGTCAGGGGGCGGATGAGGT

Downstream 100 bases:

>100_bases
AGCACCACTGTTAAATCTTAATAATGCGCTGCTACAGGGGGCGAAGGAATCATCTGCAGCTACAACCGCTGCTGTTGCGA
TTATGCCGACCAGTGAAATG

Product: DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSHKSVSAVSMLFNVGAETGVLGGLMLDNDRWDEIAPLLNSGDFYYGQHQIIFREIERLVSAGLPIDLITLSESMERKDL
LERCGGVAYLAELSKNTPSAANIVAYAEIVREYSRKRRLVKLGHELHQLASVGDADIPGLIEKAEKQLFSLAQQACDPDI
CLSVTTQVADTVSWLESVSGGSGVTGTATGFAELDEKTCGWQDGDLILLGARPSMGKTAEALKHAVAALEGSPGKTVQFY
SIEMPTQQLIMRLLSMLARVPFDNLRKGLLSEGQWSLLADAVAKLTSWEGRLLIDDTSYQTPSTLRISARRNVRKYGQPS
LILVDYLQLMSCPGRENRTQEIQEISRSLKSLAKEIRCPVAALSQLNRSVEQRQDKRPMVSDLRDSGSLEQDADVIMFLY
RDEVYNEHSPDRGIAEIILGKQRQGPLGTVKVRFEGEYMRFSEYHLGYGEVSYG

Sequences:

>Translated_454_residues
MSHKSVSAVSMLFNVGAETGVLGGLMLDNDRWDEIAPLLNSGDFYYGQHQIIFREIERLVSAGLPIDLITLSESMERKDL
LERCGGVAYLAELSKNTPSAANIVAYAEIVREYSRKRRLVKLGHELHQLASVGDADIPGLIEKAEKQLFSLAQQACDPDI
CLSVTTQVADTVSWLESVSGGSGVTGTATGFAELDEKTCGWQDGDLILLGARPSMGKTAEALKHAVAALEGSPGKTVQFY
SIEMPTQQLIMRLLSMLARVPFDNLRKGLLSEGQWSLLADAVAKLTSWEGRLLIDDTSYQTPSTLRISARRNVRKYGQPS
LILVDYLQLMSCPGRENRTQEIQEISRSLKSLAKEIRCPVAALSQLNRSVEQRQDKRPMVSDLRDSGSLEQDADVIMFLY
RDEVYNEHSPDRGIAEIILGKQRQGPLGTVKVRFEGEYMRFSEYHLGYGEVSYG
>Mature_453_residues
SHKSVSAVSMLFNVGAETGVLGGLMLDNDRWDEIAPLLNSGDFYYGQHQIIFREIERLVSAGLPIDLITLSESMERKDLL
ERCGGVAYLAELSKNTPSAANIVAYAEIVREYSRKRRLVKLGHELHQLASVGDADIPGLIEKAEKQLFSLAQQACDPDIC
LSVTTQVADTVSWLESVSGGSGVTGTATGFAELDEKTCGWQDGDLILLGARPSMGKTAEALKHAVAALEGSPGKTVQFYS
IEMPTQQLIMRLLSMLARVPFDNLRKGLLSEGQWSLLADAVAKLTSWEGRLLIDDTSYQTPSTLRISARRNVRKYGQPSL
ILVDYLQLMSCPGRENRTQEIQEISRSLKSLAKEIRCPVAALSQLNRSVEQRQDKRPMVSDLRDSGSLEQDADVIMFLYR
DEVYNEHSPDRGIAEIILGKQRQGPLGTVKVRFEGEYMRFSEYHLGYGEVSYG

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=442, Percent_Identity=49.5475113122172, Blast_Score=392, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50270; Mature: 50139

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHKSVSAVSMLFNVGAETGVLGGLMLDNDRWDEIAPLLNSGDFYYGQHQIIFREIERLV
CCCCHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHH
SAGLPIDLITLSESMERKDLLERCGGVAYLAELSKNTPSAANIVAYAEIVREYSRKRRLV
HCCCCEEEEECHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
KLGHELHQLASVGDADIPGLIEKAEKQLFSLAQQACDPDICLSVTTQVADTVSWLESVSG
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
GSGVTGTATGFAELDEKTCGWQDGDLILLGARPSMGKTAEALKHAVAALEGSPGKTVQFY
CCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEE
SIEMPTQQLIMRLLSMLARVPFDNLRKGLLSEGQWSLLADAVAKLTSWEGRLLIDDTSYQ
EECCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCC
TPSTLRISARRNVRKYGQPSLILVDYLQLMSCPGRENRTQEIQEISRSLKSLAKEIRCPV
CCCEEEEHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHH
AALSQLNRSVEQRQDKRPMVSDLRDSGSLEQDADVIMFLYRDEVYNEHSPDRGIAEIILG
HHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEEEHHHHCCCCCCCCHHHHHHHC
KQRQGPLGTVKVRFEGEYMRFSEYHLGYGEVSYG
CCCCCCCCEEEEEECCCEEEEHHHCCCCCCCCCC
>Mature Secondary Structure 
SHKSVSAVSMLFNVGAETGVLGGLMLDNDRWDEIAPLLNSGDFYYGQHQIIFREIERLV
CCCHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHH
SAGLPIDLITLSESMERKDLLERCGGVAYLAELSKNTPSAANIVAYAEIVREYSRKRRLV
HCCCCEEEEECHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
KLGHELHQLASVGDADIPGLIEKAEKQLFSLAQQACDPDICLSVTTQVADTVSWLESVSG
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
GSGVTGTATGFAELDEKTCGWQDGDLILLGARPSMGKTAEALKHAVAALEGSPGKTVQFY
CCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEE
SIEMPTQQLIMRLLSMLARVPFDNLRKGLLSEGQWSLLADAVAKLTSWEGRLLIDDTSYQ
EECCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCC
TPSTLRISARRNVRKYGQPSLILVDYLQLMSCPGRENRTQEIQEISRSLKSLAKEIRCPV
CCCEEEEHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHH
AALSQLNRSVEQRQDKRPMVSDLRDSGSLEQDADVIMFLYRDEVYNEHSPDRGIAEIILG
HHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEEEHHHHCCCCCCCCHHHHHHHC
KQRQGPLGTVKVRFEGEYMRFSEYHLGYGEVSYG
CCCCCCCCEEEEEECCCEEEEHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]