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The map label for this gene is malF [H]

Identifier: 29144396

GI number: 29144396

Start: 4277424

End: 4278968

Strand: Reverse

Name: malF [H]

Synonym: t4134

Alternate gene names: 29144396

Gene position: 4278968-4277424 (Counterclockwise)

Preceding gene: 29144397

Following gene: 29144395

Centisome position: 89.29

GC content: 52.69

Gene sequence:

>1545_bases
ATGGATGTCATTAAAAAGAAACACTGGTGGCAAAGCGACCAGCTTAAATGGTCAGTAATTGGTCTGCTGGGCCTGCTGGT
GGGTTACCTTGTAGTTTTAATGTACGTACAAGGGGAGTATCTGTTCGCCATCATGACGCTGATTTTAAGCTCTGCTGGCC
TGTATATTTTCGCAAATCGTAAAACCTATGCCTGGCGCTACGTTTACCCTGGCCTCGCCGGCATGGGGCTGTTTGTGCTA
TTCCCGCTGGTGTGCACCATCGCCATCGCCTTTACTAACTACAGCAGCACCAACCAGCTCACCTTTGAACGCGCCCAGCA
AGTGCTGATGGACCGTTCTTATCAGGCGGGAAAAACCTATAACTTCGGTCTTTACCCGGCCGGCGACGAGTGGCAACTGG
CCCTCACCGACGGAGAAACGGGCAAACATTACCTGTCTGGCGCATTTTCCTTCGGCGGCGAACAAAAATTACAGCTGAAA
GAGACCGATGCCCTGCCGGGCGGCGAACGCGCCAATCTGCGGATAATCACCCAGAACCGTCTGGCGCTGAACCAGATAAC
CGCCGTCCTGCCGGATGAAAGTAAAGTGGTTATGAGCTCGCTGCGTCAGTTTTCCGGTACCCGTCCGCTGTACACACTGG
CTGATGACGGCCTGCTTACCAATAACCAGAGCGGCGTAAAATACCGGCCAAACAACGATAGCGGTTATTATCAGTCGATC
AACGCCGACGGAAGCTGGGGTGATGAAAAACTCAGTCCTGGTTATACCGTTACTATCGGCGCGAAAAACTTTACGCGCGT
CTTTACCGACGAAGGGATCCAGAAGCCTTTTTTCGCTATCTTCGTCTGGACCGTGGTCTTTTCGGTCCTCACTGTGGTGT
TAACCGTGGCGGTTGGGATGGTATTGGCCTGCCTCGTACAGTGGGAAGCGCTGAAAGGTAAAGCTATCTACCGCGTCCTG
CTGATTCTGCCCTACGCCGTACCGTCGTTTATTTCAATTTTGATTTTCAAAGGGTTATTCAACCAAAGCTTTGGCGAAAT
CAATATGATGCTGAGCGCGCTGTTTGGCATTAAACCTGCCTGGTTCAGCGACCCCAACACCGCGCGGGCAATGGTGATTA
TCGTGAATACCTGGCTGGGCTATCCCTACATGATGATCCTGTGCATGGGACTGCTGAAAGCGATTCCGGATGACCTGTAC
GAAGCCTCCGCAATGGACGGCGCTGGTCCATTTCAGAATTTCTTTAAAATCACCTTACCGCTGCTGATTAAGCCGCTAAC
GCCGCTGATGATCGCCAGCTTCGCCTTTAACTTTAATAACTTTGTCCTGATTCAGTTATTGACCAACGGCGGGCCAGACC
GTCTCGGCACCACCACGCCTGCCGGTTATACCGATCTGCTCGTCAGCTACACCTACCGTATCGCCTTTGAAGGCGGCGGC
GGTCAGGACTTCGGTCTGGCGGCGGCCATTGCCACGCTGATCTTCCTGCTGGTAGGCGCGCTGGCAATAGTGAACCTGAA
AGCCACGCGTATGAAGTTTGATTAA

Upstream 100 bases:

>100_bases
ATGCCTTATCCGGCCTACAACACACCGTAGGCCTGATAAGCGCAGCGCCATCAGGCACATCTCCGGCCAGGAAGTTTCAA
CGCTGTAGAGGAAAACCCCC

Downstream 100 bases:

>100_bases
GGGAGATAATAACAATGGCTATGGTCCAACCCAAATCTCAGAAACTGCGTCTCTTCATCACGCACCTGGGGCTGCTGATT
TTCATCGCGGCGATTATGTT

Product: maltose transporter membrane protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MDVIKKKHWWQSDQLKWSVIGLLGLLVGYLVVLMYVQGEYLFAIMTLILSSAGLYIFANRKTYAWRYVYPGLAGMGLFVL
FPLVCTIAIAFTNYSSTNQLTFERAQQVLMDRSYQAGKTYNFGLYPAGDEWQLALTDGETGKHYLSGAFSFGGEQKLQLK
ETDALPGGERANLRIITQNRLALNQITAVLPDESKVVMSSLRQFSGTRPLYTLADDGLLTNNQSGVKYRPNNDSGYYQSI
NADGSWGDEKLSPGYTVTIGAKNFTRVFTDEGIQKPFFAIFVWTVVFSVLTVVLTVAVGMVLACLVQWEALKGKAIYRVL
LILPYAVPSFISILIFKGLFNQSFGEINMMLSALFGIKPAWFSDPNTARAMVIIVNTWLGYPYMMILCMGLLKAIPDDLY
EASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNGGPDRLGTTTPAGYTDLLVSYTYRIAFEGGG
GQDFGLAAAIATLIFLLVGALAIVNLKATRMKFD

Sequences:

>Translated_514_residues
MDVIKKKHWWQSDQLKWSVIGLLGLLVGYLVVLMYVQGEYLFAIMTLILSSAGLYIFANRKTYAWRYVYPGLAGMGLFVL
FPLVCTIAIAFTNYSSTNQLTFERAQQVLMDRSYQAGKTYNFGLYPAGDEWQLALTDGETGKHYLSGAFSFGGEQKLQLK
ETDALPGGERANLRIITQNRLALNQITAVLPDESKVVMSSLRQFSGTRPLYTLADDGLLTNNQSGVKYRPNNDSGYYQSI
NADGSWGDEKLSPGYTVTIGAKNFTRVFTDEGIQKPFFAIFVWTVVFSVLTVVLTVAVGMVLACLVQWEALKGKAIYRVL
LILPYAVPSFISILIFKGLFNQSFGEINMMLSALFGIKPAWFSDPNTARAMVIIVNTWLGYPYMMILCMGLLKAIPDDLY
EASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNGGPDRLGTTTPAGYTDLLVSYTYRIAFEGGG
GQDFGLAAAIATLIFLLVGALAIVNLKATRMKFD
>Mature_514_residues
MDVIKKKHWWQSDQLKWSVIGLLGLLVGYLVVLMYVQGEYLFAIMTLILSSAGLYIFANRKTYAWRYVYPGLAGMGLFVL
FPLVCTIAIAFTNYSSTNQLTFERAQQVLMDRSYQAGKTYNFGLYPAGDEWQLALTDGETGKHYLSGAFSFGGEQKLQLK
ETDALPGGERANLRIITQNRLALNQITAVLPDESKVVMSSLRQFSGTRPLYTLADDGLLTNNQSGVKYRPNNDSGYYQSI
NADGSWGDEKLSPGYTVTIGAKNFTRVFTDEGIQKPFFAIFVWTVVFSVLTVVLTVAVGMVLACLVQWEALKGKAIYRVL
LILPYAVPSFISILIFKGLFNQSFGEINMMLSALFGIKPAWFSDPNTARAMVIIVNTWLGYPYMMILCMGLLKAIPDDLY
EASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNGGPDRLGTTTPAGYTDLLVSYTYRIAFEGGG
GQDFGLAAAIATLIFLLVGALAIVNLKATRMKFD

Specific function: Part of the binding-protein-dependent transport system for maltose; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1175

COG function: function code G; ABC-type sugar transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein; Periplasmic side. Note=A substantial portion of it protrudes into the periplasmic space [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1790465, Length=514, Percent_Identity=91.0505836575876, Blast_Score=927, Evalue=0.0,
Organism=Escherichia coli, GI1787570, Length=276, Percent_Identity=27.536231884058, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1789861, Length=259, Percent_Identity=32.046332046332, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 56866; Mature: 56866

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVIKKKHWWQSDQLKWSVIGLLGLLVGYLVVLMYVQGEYLFAIMTLILSSAGLYIFANR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECC
KTYAWRYVYPGLAGMGLFVLFPLVCTIAIAFTNYSSTNQLTFERAQQVLMDRSYQAGKTY
CEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEE
NFGLYPAGDEWQLALTDGETGKHYLSGAFSFGGEQKLQLKETDALPGGERANLRIITQNR
CCCEECCCCCEEEEEECCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEECCC
LALNQITAVLPDESKVVMSSLRQFSGTRPLYTLADDGLLTNNQSGVKYRPNNDSGYYQSI
HHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCEEECCCCCCEECCCCCCCCEEEE
NADGSWGDEKLSPGYTVTIGAKNFTRVFTDEGIQKPFFAIFVWTVVFSVLTVVLTVAVGM
CCCCCCCCCCCCCCEEEEECCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VLACLVQWEALKGKAIYRVLLILPYAVPSFISILIFKGLFNQSFGEINMMLSALFGIKPA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
WFSDPNTARAMVIIVNTWLGYPYMMILCMGLLKAIPDDLYEASAMDGAGPFQNFFKITLP
CCCCCCCCEEEEEEEECHHCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHH
LLIKPLTPLMIASFAFNFNNFVLIQLLTNGGPDRLGTTTPAGYTDLLVSYTYRIAFEGGG
HHHCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHEEEEEEEEECCC
GQDFGLAAAIATLIFLLVGALAIVNLKATRMKFD
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHEECCC
>Mature Secondary Structure
MDVIKKKHWWQSDQLKWSVIGLLGLLVGYLVVLMYVQGEYLFAIMTLILSSAGLYIFANR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECC
KTYAWRYVYPGLAGMGLFVLFPLVCTIAIAFTNYSSTNQLTFERAQQVLMDRSYQAGKTY
CEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEE
NFGLYPAGDEWQLALTDGETGKHYLSGAFSFGGEQKLQLKETDALPGGERANLRIITQNR
CCCEECCCCCEEEEEECCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEECCC
LALNQITAVLPDESKVVMSSLRQFSGTRPLYTLADDGLLTNNQSGVKYRPNNDSGYYQSI
HHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCEEECCCCCCEECCCCCCCCEEEE
NADGSWGDEKLSPGYTVTIGAKNFTRVFTDEGIQKPFFAIFVWTVVFSVLTVVLTVAVGM
CCCCCCCCCCCCCCEEEEECCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VLACLVQWEALKGKAIYRVLLILPYAVPSFISILIFKGLFNQSFGEINMMLSALFGIKPA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
WFSDPNTARAMVIIVNTWLGYPYMMILCMGLLKAIPDDLYEASAMDGAGPFQNFFKITLP
CCCCCCCCEEEEEEEECHHCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHH
LLIKPLTPLMIASFAFNFNNFVLIQLLTNGGPDRLGTTTPAGYTDLLVSYTYRIAFEGGG
HHHCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHEEEEEEEEECCC
GQDFGLAAAIATLIFLLVGALAIVNLKATRMKFD
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2674653 [H]