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The map label for this gene is lysC [H]

Identifier: 29144388

GI number: 29144388

Start: 4268198

End: 4269547

Strand: Reverse

Name: lysC [H]

Synonym: t4126

Alternate gene names: 29144388

Gene position: 4269547-4268198 (Counterclockwise)

Preceding gene: 29144395

Following gene: 29144387

Centisome position: 89.1

GC content: 56.81

Gene sequence:

>1350_bases
ATGACAGAGATCGTTGTTTCCAAATTTGGCGGTACCAGCGTCGCTGATTTTGACGCCATGAACCGTAGCGCGGATATCGT
ACTTTCCGACGCCAATGTTCGTTTAGTCGTGCTTTCCGCGTCTGCCGGCATCACTAATCTGCTGGTGGCGCTGGCCGAAG
GGATGGAGCCCGGCGAACGTTTCGCTACGCTCGACGCCATCCGTAAAATCCAGTTCGATATCCTTGATCGTCTGCGTCAT
CCGAATGTCATTCGCGAAGAGATTGAACGCCTGCTGGAAAACATCACTATTCTGGCGGAGGCGGCATCGCTGGCGACATC
GGCAGCCCTGACCGATGAACTGGTCAGCCACGGCGAGCTAATGTCTACCCTGCTATTTGTTGAGATCCTGCGTGAACGCG
ACGTACAGGCCCACTGGTTCGACGTGCGCAAAGTGATGCGTACCAGCGATCGCTTTGGTCGTGCGGAACCGGACGTCGCA
GCGCTTGCGGAACTGGCCGCGCAGCAGTTGCTCCCGCGCCTGAGCGAAACGTTGGTCATTACTCAGGGCTTTATCGGCAG
TGAAAGCAAAGGCCGCACCACGACATTAGGCCGCGGCGGCAGCGACTATACGGCAGCGTTGCTGGCTGAAGCGCTCCACG
CCGCGCGGGTTGATATCTGGACAGATGTGCCCGGTATTTACACCACCGACCCGCGCGTAGTGCCCGTCGCGCAGCGAATT
GATGAAATCGACTTTGAAGAAGCGGCGGAGATGGCAACCTTCGGCGCAAAAGTACTGCATCCAGCCACCTTACTCCCGGC
CGTGCGCAGCGATATCCCGGTCTTCGTTGGCTCCAGTAAAGATCCGCAAGCGGGCGGTACGCTGGTGTGCAATAAAACCC
AAAACCCGCCGCTGTTCCGCGCGCTGGCGCTGCGCCGCAATCAAACGCTATTGACGCTGCACAGCCTGAATATGCTGCAC
TCACGAGGTTTCCTGGCAGAGGTGTTCGGCATTCTGGCGCGGCATAATATTTCGGTAGATCTGATTACCACTTCGGAGGT
GAGCGTCGCGCTGACGCTGGATACTACCGGCTCCACCTCCACGGGCGATACGTTACTGACCCAATCCTTATTGATGGAGC
TTTCCGCGCTGTGTCGGGTCGAAGTTGAAGAAGGACTGGCGCTGGTCGCGCTGATTGGCAACAACCTGTCAAAAGCCTGC
GGTGTTGGTAAAGAAGTGTTTGGCGTCCTGGAACCGTTCAACATTCGCATGATTTGTTACGGCGCCTCCAGCCACAATCT
GTGCTTCCTCGTGCCGGGAGACGATGCCGAGAAAGTGGTGCAAAAGCTACATCAGAACTTATTTGAATAA

Upstream 100 bases:

>100_bases
CTTTTATTTATAAATACGTTACTTGCATTTGCGCCTGCACCGGTTTCTGTTCTGCCTTTGTGCCATGGTCGCGTTTTTCA
TTTCCTGACACGAGGTTGAT

Downstream 100 bases:

>100_bases
TCGCCCTGACACGACAGCGGGTGAGCGCTATCAGGCAATACGCTGCCGGATGGCGACGCAAGCGCCTTATCCGGCGGTTC
CCACGTCAGGCACTGCCGGC

Product: aspartate kinase III

Products: NA

Alternate protein names: Aspartate kinase III; AKIII; Lysine-sensitive aspartokinase III [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MTEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGITNLLVALAEGMEPGERFATLDAIRKIQFDILDRLRH
PNVIREEIERLLENITILAEAASLATSAALTDELVSHGELMSTLLFVEILRERDVQAHWFDVRKVMRTSDRFGRAEPDVA
ALAELAAQQLLPRLSETLVITQGFIGSESKGRTTTLGRGGSDYTAALLAEALHAARVDIWTDVPGIYTTDPRVVPVAQRI
DEIDFEEAAEMATFGAKVLHPATLLPAVRSDIPVFVGSSKDPQAGGTLVCNKTQNPPLFRALALRRNQTLLTLHSLNMLH
SRGFLAEVFGILARHNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALIGNNLSKAC
GVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGDDAEKVVQKLHQNLFE

Sequences:

>Translated_449_residues
MTEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGITNLLVALAEGMEPGERFATLDAIRKIQFDILDRLRH
PNVIREEIERLLENITILAEAASLATSAALTDELVSHGELMSTLLFVEILRERDVQAHWFDVRKVMRTSDRFGRAEPDVA
ALAELAAQQLLPRLSETLVITQGFIGSESKGRTTTLGRGGSDYTAALLAEALHAARVDIWTDVPGIYTTDPRVVPVAQRI
DEIDFEEAAEMATFGAKVLHPATLLPAVRSDIPVFVGSSKDPQAGGTLVCNKTQNPPLFRALALRRNQTLLTLHSLNMLH
SRGFLAEVFGILARHNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALIGNNLSKAC
GVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGDDAEKVVQKLHQNLFE
>Mature_448_residues
TEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGITNLLVALAEGMEPGERFATLDAIRKIQFDILDRLRHP
NVIREEIERLLENITILAEAASLATSAALTDELVSHGELMSTLLFVEILRERDVQAHWFDVRKVMRTSDRFGRAEPDVAA
LAELAAQQLLPRLSETLVITQGFIGSESKGRTTTLGRGGSDYTAALLAEALHAARVDIWTDVPGIYTTDPRVVPVAQRID
EIDFEEAAEMATFGAKVLHPATLLPAVRSDIPVFVGSSKDPQAGGTLVCNKTQNPPLFRALALRRNQTLLTLHSLNMLHS
RGFLAEVFGILARHNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALIGNNLSKACG
VGKEVFGVLEPFNIRMICYGASSHNLCFLVPGDDAEKVVQKLHQNLFE

Specific function: Lysine biosynthesis; first step. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Escherichia coli, GI1790455, Length=449, Percent_Identity=93.3184855233853, Blast_Score=781, Evalue=0.0,
Organism=Escherichia coli, GI1786183, Length=466, Percent_Identity=31.3304721030043, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI1790376, Length=454, Percent_Identity=26.6519823788546, Blast_Score=122, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6320893, Length=500, Percent_Identity=27.4, Blast_Score=164, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 48595; Mature: 48464

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGITNLLVALAEGMEPGER
CCCEEEECCCCCCCHHHHHCCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHHCCCCHHH
FATLDAIRKIQFDILDRLRHPNVIREEIERLLENITILAEAASLATSAALTDELVSHGEL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSTLLFVEILRERDVQAHWFDVRKVMRTSDRFGRAEPDVAALAELAAQQLLPRLSETLVI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEE
TQGFIGSESKGRTTTLGRGGSDYTAALLAEALHAARVDIWTDVPGIYTTDPRVVPVAQRI
EECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHEEEECCCCCEECCCCEEHHHHHH
DEIDFEEAAEMATFGAKVLHPATLLPAVRSDIPVFVGSSKDPQAGGTLVCNKTQNPPLFR
CCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEECCCCCCCCCCEEEECCCCCCHHHH
ALALRRNQTLLTLHSLNMLHSRGFLAEVFGILARHNISVDLITTSEVSVALTLDTTGSTS
HHHHHCCCEEEEEHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEECCEEEEEEEECCCCCC
TGDTLLTQSLLMELSALCRVEVEEGLALVALIGNNLSKACGVGKEVFGVLEPFNIRMICY
CCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHCCCHHHHHHCCCCCEEEEEE
GASSHNLCFLVPGDDAEKVVQKLHQNLFE
ECCCCCEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGITNLLVALAEGMEPGER
CCEEEECCCCCCCHHHHHCCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHHCCCCHHH
FATLDAIRKIQFDILDRLRHPNVIREEIERLLENITILAEAASLATSAALTDELVSHGEL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSTLLFVEILRERDVQAHWFDVRKVMRTSDRFGRAEPDVAALAELAAQQLLPRLSETLVI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEE
TQGFIGSESKGRTTTLGRGGSDYTAALLAEALHAARVDIWTDVPGIYTTDPRVVPVAQRI
EECCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHEEEECCCCCEECCCCEEHHHHHH
DEIDFEEAAEMATFGAKVLHPATLLPAVRSDIPVFVGSSKDPQAGGTLVCNKTQNPPLFR
CCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEECCCCCCCCCCEEEECCCCCCHHHH
ALALRRNQTLLTLHSLNMLHSRGFLAEVFGILARHNISVDLITTSEVSVALTLDTTGSTS
HHHHHCCCEEEEEHHHHHHHHCCHHHHHHHHHHHCCCEEEEEEECCEEEEEEEECCCCCC
TGDTLLTQSLLMELSALCRVEVEEGLALVALIGNNLSKACGVGKEVFGVLEPFNIRMICY
CCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHCCCHHHHHHCCCCCEEEEEE
GASSHNLCFLVPGDDAEKVVQKLHQNLFE
ECCCCCEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3003049; 8265357; 9278503; 6312411 [H]