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The map label for this gene is iclR [H]

Identifier: 29144376

GI number: 29144376

Start: 4254251

End: 4255075

Strand: Reverse

Name: iclR [H]

Synonym: t4114

Alternate gene names: 29144376

Gene position: 4255075-4254251 (Counterclockwise)

Preceding gene: 29144379

Following gene: 29144371

Centisome position: 88.8

GC content: 57.94

Gene sequence:

>825_bases
ATGGTTGCCCCTGTTCCCGCAAAACGCGGGAGAAAACCTGCCGCTACCACTGCACCCGTAACCGGGCAGGTTCAGTCTTT
AACTCGCGGTCTGAAGCTGCTGGAGTGGATTGCCGAGTCCAACGGCAGCGTCGCGCTGACCGAACTGGCGCAACAGGCGG
GTTTGCCTAATTCCACGACTCACCGTCTACTCACCACCATGCAGCAACAGGGTTTTGTCCGCCAGGTTGGTGAGCTGGGC
CACTGGGCCGTAGGCGCACACGCGTTTATCGTCGGTAGCAGCTTCTTACAGAGCCGTAATCTGCTGGCGATTGTCCATCC
GATTTTGCGCAAGCTGATGGAGGACTCCGGCGAAACGGTAAACCTTGCCGTACTCGACCAGAGCGACCATCAGGCTATTA
TTATCGACCAGGTGCAGTGCACACAATTGATGCGCATGTCCGCCCCTATTGGCGGCAAGCTGCCCATGCACGCCTCTGGC
GCGGGCAAGGCGTTTTTATCACAGCTCAGTGAGGAACAGGTTACCAGTCTGCTGCACCGCAAGGGGTTGCACGCTTATAC
CCATGCGACGCTGGTGTCGCCGCTGCATCTGAAAGATGACCTCGCCCAAACACGCAAGCGCGGCTATTCCTTTGACGACG
AAGAACATGCGCTCGGTCTGCGCTGCGTGGCCTCGTGTATTTATGACGAACACCGCGAACCGTTTGCGGCCCTCTCTATT
TCCGGCCCCTGCTCACGCATTACCGACGATCGCGTCACCGAACTTGGCGCCATGGTGATTAAAGCGGCGAAAGAGGTGAC
GCTGGCCTATGGTGGGTTTAGATAA

Upstream 100 bases:

>100_bases
GGCATCCTGTGATGTCACTCATCCGATCAGTTCAGGAACTCTTGCATTATAATTGAATAAAACGAAAATGATTTCCACGA
TACAGAAAAAGGAGTCTGCC

Downstream 100 bases:

>100_bases
GCAAGGGTTGGAACTGATTTGTAGGCCTGATAAGCGCAGCGCCATCAGGCACGGCGTACATTGCCGGATGGCGGCGCAAA
GGCCTTATCCGGCCTACGGT

Product: transcriptional repressor IclR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MVAPVPAKRGRKPAATTAPVTGQVQSLTRGLKLLEWIAESNGSVALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELG
HWAVGAHAFIVGSSFLQSRNLLAIVHPILRKLMEDSGETVNLAVLDQSDHQAIIIDQVQCTQLMRMSAPIGGKLPMHASG
AGKAFLSQLSEEQVTSLLHRKGLHAYTHATLVSPLHLKDDLAQTRKRGYSFDDEEHALGLRCVASCIYDEHREPFAALSI
SGPCSRITDDRVTELGAMVIKAAKEVTLAYGGFR

Sequences:

>Translated_274_residues
MVAPVPAKRGRKPAATTAPVTGQVQSLTRGLKLLEWIAESNGSVALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELG
HWAVGAHAFIVGSSFLQSRNLLAIVHPILRKLMEDSGETVNLAVLDQSDHQAIIIDQVQCTQLMRMSAPIGGKLPMHASG
AGKAFLSQLSEEQVTSLLHRKGLHAYTHATLVSPLHLKDDLAQTRKRGYSFDDEEHALGLRCVASCIYDEHREPFAALSI
SGPCSRITDDRVTELGAMVIKAAKEVTLAYGGFR
>Mature_274_residues
MVAPVPAKRGRKPAATTAPVTGQVQSLTRGLKLLEWIAESNGSVALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELG
HWAVGAHAFIVGSSFLQSRNLLAIVHPILRKLMEDSGETVNLAVLDQSDHQAIIIDQVQCTQLMRMSAPIGGKLPMHASG
AGKAFLSQLSEEQVTSLLHRKGLHAYTHATLVSPLHLKDDLAQTRKRGYSFDDEEHALGLRCVASCIYDEHREPFAALSI
SGPCSRITDDRVTELGAMVIKAAKEVTLAYGGFR

Specific function: Regulation of the glyoxylate bypass operon (aceBAK), which encodes isocitrate lyase, malate synthase as well as isocitrate dehydrogenase kinase/phosphorylase. Glyoxylate disrupts the interaction with the promoter by favoring the inactive dimeric form. Pyr

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI87082359, Length=274, Percent_Identity=91.970802919708, Blast_Score=530, Evalue=1e-152,
Organism=Escherichia coli, GI1786715, Length=264, Percent_Identity=42.0454545454545, Blast_Score=222, Evalue=2e-59,
Organism=Escherichia coli, GI1786468, Length=253, Percent_Identity=30.4347826086957, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788131, Length=248, Percent_Identity=29.8387096774194, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1789999, Length=227, Percent_Identity=33.4801762114537, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788581, Length=251, Percent_Identity=24.3027888446215, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790752, Length=252, Percent_Identity=25.3968253968254, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 29660; Mature: 29660

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAPVPAKRGRKPAATTAPVTGQVQSLTRGLKLLEWIAESNGSVALTELAQQAGLPNSTT
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHH
HRLLTTMQQQGFVRQVGELGHWAVGAHAFIVGSSFLQSRNLLAIVHPILRKLMEDSGETV
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEE
NLAVLDQSDHQAIIIDQVQCTQLMRMSAPIGGKLPMHASGAGKAFLSQLSEEQVTSLLHR
EEEEEECCCCCEEEECHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
KGLHAYTHATLVSPLHLKDDLAQTRKRGYSFDDEEHALGLRCVASCIYDEHREPFAALSI
CCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
SGPCSRITDDRVTELGAMVIKAAKEVTLAYGGFR
CCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVAPVPAKRGRKPAATTAPVTGQVQSLTRGLKLLEWIAESNGSVALTELAQQAGLPNSTT
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHH
HRLLTTMQQQGFVRQVGELGHWAVGAHAFIVGSSFLQSRNLLAIVHPILRKLMEDSGETV
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEE
NLAVLDQSDHQAIIIDQVQCTQLMRMSAPIGGKLPMHASGAGKAFLSQLSEEQVTSLLHR
EEEEEECCCCCEEEECHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
KGLHAYTHATLVSPLHLKDDLAQTRKRGYSFDDEEHALGLRCVASCIYDEHREPFAALSI
CCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
SGPCSRITDDRVTELGAMVIKAAKEVTLAYGGFR
CCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185227; 1995431; 8265357; 9278503; 1995429 [H]