Click here to switch to the map view.

The map label for this gene is bioH [H]

Identifier: 29144265

GI number: 29144265

Start: 4146568

End: 4147338

Strand: Direct

Name: bioH [H]

Synonym: t3997

Alternate gene names: 29144265

Gene position: 4146568-4147338 (Clockwise)

Preceding gene: 29144261

Following gene: 29144272

Centisome position: 86.53

GC content: 56.81

Gene sequence:

>771_bases
ATGAATGACATCTGGTGGCAGACCTACGGCGAGGGAAATTGTCATCTTGTGCTGCTGCACGGATGGGGATTGAACGCGGA
GGTGTGGCATTGCATTCGCGAGGAACTCGGCTCGCATTTTACGCTGCATCTGGTCGACTTGCCGGGCTATGGCCGTAGTT
CGGGGTTTGGTGCCATGACGCTTGAAGAGATGACGGCGCAGGTAGCGAAAAACGCGCCGGACCAGGCTATCTGGCTTGGC
TGGAGTCTGGGCGGCCTGGTGGCGAGTCAGATGGCGCTCACCCACCCTGAACGCGTTCAGGCGCTGGTGACAGTCGCCTC
TTCGCCATGCTTTAGCGCGCGTGAGGGGTGGCCGGGAATAAAACCAGAAATCCTCGGCGGATTCCAGCAGCAGCTTAGCG
ACGATTTTCAGCGCACGGTGGAGCGTTTTCTGGCGCTGCAAACGTTAGGGACGGAGACGGCGCGTCAGGATGCCCGCACC
TTAAAAAGCGTAGTGCTGGCGCAGCCTATGCCGGATGTAGAGGTGCTCAATGGCGGACTGGAAATCTTAAAAACGGTCGA
TCTACGAGAAGCGCTTAAAAATGTGAACATGCCGTTTTTGCGTTTGTATGGTTATCTGGACGGTCTGGTGCCGCGTAAAA
TCGCGCCTTTGCTCGATACGTTATGGCCGCACAGTACATCACAGATAATGGCGAAGGCGGCCCATGCGCCGTTTATCTCG
CATCCGGCGGCGTTTTGCCAGGCGCTGATGACGCTAAAATCATCGCTGTAA

Upstream 100 bases:

>100_bases
CCCCCAGTGGCTTAACGCCAGCGGCATTCGGCATAGCCAGCATAATCCCGGTACTGTTAGCATAGGTGCATCCTTGTTAG
ACAAAAGAGAACAGTAACTG

Downstream 100 bases:

>100_bases
CCAGGGCGTTGCCGGGCCCAGGCCATTCTGCCAGATTAATGGCTTTCAGACGCCAGATCGGCAGGCGCCAGCCCGGTAAC
CCTAAGCACAGTATCGCGAT

Product: carboxylesterase BioH

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MNDIWWQTYGEGNCHLVLLHGWGLNAEVWHCIREELGSHFTLHLVDLPGYGRSSGFGAMTLEEMTAQVAKNAPDQAIWLG
WSLGGLVASQMALTHPERVQALVTVASSPCFSAREGWPGIKPEILGGFQQQLSDDFQRTVERFLALQTLGTETARQDART
LKSVVLAQPMPDVEVLNGGLEILKTVDLREALKNVNMPFLRLYGYLDGLVPRKIAPLLDTLWPHSTSQIMAKAAHAPFIS
HPAAFCQALMTLKSSL

Sequences:

>Translated_256_residues
MNDIWWQTYGEGNCHLVLLHGWGLNAEVWHCIREELGSHFTLHLVDLPGYGRSSGFGAMTLEEMTAQVAKNAPDQAIWLG
WSLGGLVASQMALTHPERVQALVTVASSPCFSAREGWPGIKPEILGGFQQQLSDDFQRTVERFLALQTLGTETARQDART
LKSVVLAQPMPDVEVLNGGLEILKTVDLREALKNVNMPFLRLYGYLDGLVPRKIAPLLDTLWPHSTSQIMAKAAHAPFIS
HPAAFCQALMTLKSSL
>Mature_256_residues
MNDIWWQTYGEGNCHLVLLHGWGLNAEVWHCIREELGSHFTLHLVDLPGYGRSSGFGAMTLEEMTAQVAKNAPDQAIWLG
WSLGGLVASQMALTHPERVQALVTVASSPCFSAREGWPGIKPEILGGFQQQLSDDFQRTVERFLALQTLGTETARQDART
LKSVVLAQPMPDVEVLNGGLEILKTVDLREALKNVNMPFLRLYGYLDGLVPRKIAPLLDTLWPHSTSQIMAKAAHAPFIS
HPAAFCQALMTLKSSL

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=256, Percent_Identity=79.6875, Blast_Score=434, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 28241; Mature: 28241

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDIWWQTYGEGNCHLVLLHGWGLNAEVWHCIREELGSHFTLHLVDLPGYGRSSGFGAMT
CCCCCEEECCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHH
LEEMTAQVAKNAPDQAIWLGWSLGGLVASQMALTHPERVQALVTVASSPCFSAREGWPGI
HHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC
KPEILGGFQQQLSDDFQRTVERFLALQTLGTETARQDARTLKSVVLAQPMPDVEVLNGGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
EILKTVDLREALKNVNMPFLRLYGYLDGLVPRKIAPLLDTLWPHSTSQIMAKAAHAPFIS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCC
HPAAFCQALMTLKSSL
CHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNDIWWQTYGEGNCHLVLLHGWGLNAEVWHCIREELGSHFTLHLVDLPGYGRSSGFGAMT
CCCCCEEECCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHH
LEEMTAQVAKNAPDQAIWLGWSLGGLVASQMALTHPERVQALVTVASSPCFSAREGWPGI
HHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC
KPEILGGFQQQLSDDFQRTVERFLALQTLGTETARQDARTLKSVVLAQPMPDVEVLNGGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
EILKTVDLREALKNVNMPFLRLYGYLDGLVPRKIAPLLDTLWPHSTSQIMAKAAHAPFIS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCC
HPAAFCQALMTLKSSL
CHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA