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The map label for this gene is glgX [H]

Identifier: 29144251

GI number: 29144251

Start: 4124488

End: 4126452

Strand: Direct

Name: glgX [H]

Synonym: t3983

Alternate gene names: 29144251

Gene position: 4124488-4126452 (Clockwise)

Preceding gene: 29144250

Following gene: 29144252

Centisome position: 86.07

GC content: 56.84

Gene sequence:

>1965_bases
ATGACGCAGCTTGCGATCGGCGAAGCAACGCCGCATGGCGCAACGTATGACGGTCATGGCGTGAATTTCACGCTCTTTTC
CGCCCATGCGGAGCGCGTAGAGCTGTGCGTTTTTGATTCCCGAGGGAATGAGCGTCGCTATGACCTGCCAGGACGTCGCG
GCGATGTCTGGCATGGTTATCTGGCAGGCGCCAGGCCAGGTTTGCGCTATGGCTATCGCGTACATGGCCCGTGGCAGCCA
GCGCAGGGGCATCGGTTTAATCCGGCGAAGTTACTGCTTGACCCTTATGCGCGCCAAGTCGAGGGCGAACTAAAAGATCA
TCCGCTGTTGCACGGCGGACACGATGAACCGGACTACCGCGATAACGCGGCGGTGGCGCCGAAAAGCGTGGTAATCAGCG
ACCATTATGACTGGGAAGATGACGCCGCGCCGCGCACGCCGTGGGGAAAAACGGTCATCTATGAAGCGCATGTCAAAGGG
CTTACCTATCTGCATCCGGAACTCCCCCAGGAGATACGCGGCACCTATAAAGCGCTCGGTCATCCGGTCATGGTGGCGTA
CTTCAAACAGCTTGGCATTACGGCGCTGGAACTGTTGCCGGTGGCGCAGTTTGCCAGCGAGCCGCGTCTGCAACGTATGG
GGCTGACCAATTATTGGGGTTATAACCCAATGGCGATGTTTGCGCTGCATCCCGCCTGGGCCAGTTCGCCCGAGACGGCG
CTGGACGAGTTTCGCGATGCGGTGAAAGCGCTGCATCGCGCGGGGATTGAGGTCATTCTGGACATCGTATTAAACCATAG
CGCCGAGCTGGATCTGGACGGTCCAACCTTCTCCCTGCGCGGAATTGATAACCGTAGCTATTATTGGATAAGGGACGATG
GCGATTATCACAACTGGACGGGGTGCGGCAACACGCTCAATTTAAGCCATCCCGGCGTGGTGGAATACGCGTGCGAGTGT
TTGCGCTATTGGATGGAAACCTGTCATGTCGATGGTTTTCGTTTTGATTTGGCGTCCGTCATGGGGCGCACGCCGACGTT
TCGCCAGGATGCGCCGCTGTTCGCCGCGATTAAAGCGTGTCCTGTGTTATCAACGGTCAAGCTGATTGCTGAACCGTGGG
ATATTGGCGAGGGCGGCTATCAGGTCGGGAATTTTCCGCCACCGTTTGCCGAGTGGAACGATCATTTTCGCGATGCGGCC
CGCCGATTCTGGTTGCCGCGTAACCTGACGACCGGGGAGTTTGCCTGTCGTTTCGCCGCTTCCAGCGATGTGTTTAAACG
CAATGGCCGCGCGCCCGGCGCCTCTGTCAATCTGCTCACGGCGCATGACGGTTTTACGCTCCGGGACTGCGTCTGTTTCA
ATCAGAAACACAATGAGGCGAACGGTGAGGAAAATCGCGACGGCACCAACAGCAATTACAGCGACAATCATGGTAAAGAA
GGATTAGGCGGTCCGCTTGATTTAATGGAGCGGCGGCGCGACAGCATTCATGCCCTGCTGGCGACGCTACTGCTCTCTCA
GGGAACGCCGATGCTGCTGGCAGGCGATGAACACGGCCATAGCCAGCACGGCAACAATAACGCCTACTGTCAGGATAATG
CCTTAACCTGGCTGGACTGGCAGCAGGCAAATCGTGGGTTAACCACGTTTACCGCCGCGCTGATTCGTTTGCGTCAGCAG
ATACCGGCTTTAACCGGCAATAGCTGGTGGGAAGAAGGCGATGGCAACGTGCGTTGGCTAAATAAAAACGCGCAACCCTT
GAGTGCGGATGAGTGGCAAAACGGACCTAAGCTGATGCAAATTCTGTTGTCGGACCGTTTTCTGATTGCGATAAATGCCA
CGCTTGAGGTGACAGATATAGTTTTACCCGAAGGGGAATGGCGCGCCGTTCCCCCATTCGCTGGAGAGGATAATCCGGTG
ATAACGGCGGTCTGGCAGGGACTGTGTGTGTTCCAGAGAGGATAA

Upstream 100 bases:

>100_bases
GTGTGGTGCACAGCGATGAGATCGAAAGCCATGGCCGTCAGCACTCTCTTAACCTGACGTTACCGCCGTTGGCGACGATC
TGGCTGATGCGGGAGGGGGA

Downstream 100 bases:

>100_bases
AAAAGGAGTTAATCATGGTGAGTTTAGAGAAGAACGATCGTGTAATGTTGGCGCGCCAGCTGCCATTGAAATCTGTTGCC
CTAATCCTGGCCGGCGGCCG

Product: glycogen debranching protein

Products: NA

Alternate protein names: Glycogen operon protein GlgX [H]

Number of amino acids: Translated: 654; Mature: 653

Protein sequence:

>654_residues
MTQLAIGEATPHGATYDGHGVNFTLFSAHAERVELCVFDSRGNERRYDLPGRRGDVWHGYLAGARPGLRYGYRVHGPWQP
AQGHRFNPAKLLLDPYARQVEGELKDHPLLHGGHDEPDYRDNAAVAPKSVVISDHYDWEDDAAPRTPWGKTVIYEAHVKG
LTYLHPELPQEIRGTYKALGHPVMVAYFKQLGITALELLPVAQFASEPRLQRMGLTNYWGYNPMAMFALHPAWASSPETA
LDEFRDAVKALHRAGIEVILDIVLNHSAELDLDGPTFSLRGIDNRSYYWIRDDGDYHNWTGCGNTLNLSHPGVVEYACEC
LRYWMETCHVDGFRFDLASVMGRTPTFRQDAPLFAAIKACPVLSTVKLIAEPWDIGEGGYQVGNFPPPFAEWNDHFRDAA
RRFWLPRNLTTGEFACRFAASSDVFKRNGRAPGASVNLLTAHDGFTLRDCVCFNQKHNEANGEENRDGTNSNYSDNHGKE
GLGGPLDLMERRRDSIHALLATLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNALTWLDWQQANRGLTTFTAALIRLRQQ
IPALTGNSWWEEGDGNVRWLNKNAQPLSADEWQNGPKLMQILLSDRFLIAINATLEVTDIVLPEGEWRAVPPFAGEDNPV
ITAVWQGLCVFQRG

Sequences:

>Translated_654_residues
MTQLAIGEATPHGATYDGHGVNFTLFSAHAERVELCVFDSRGNERRYDLPGRRGDVWHGYLAGARPGLRYGYRVHGPWQP
AQGHRFNPAKLLLDPYARQVEGELKDHPLLHGGHDEPDYRDNAAVAPKSVVISDHYDWEDDAAPRTPWGKTVIYEAHVKG
LTYLHPELPQEIRGTYKALGHPVMVAYFKQLGITALELLPVAQFASEPRLQRMGLTNYWGYNPMAMFALHPAWASSPETA
LDEFRDAVKALHRAGIEVILDIVLNHSAELDLDGPTFSLRGIDNRSYYWIRDDGDYHNWTGCGNTLNLSHPGVVEYACEC
LRYWMETCHVDGFRFDLASVMGRTPTFRQDAPLFAAIKACPVLSTVKLIAEPWDIGEGGYQVGNFPPPFAEWNDHFRDAA
RRFWLPRNLTTGEFACRFAASSDVFKRNGRAPGASVNLLTAHDGFTLRDCVCFNQKHNEANGEENRDGTNSNYSDNHGKE
GLGGPLDLMERRRDSIHALLATLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNALTWLDWQQANRGLTTFTAALIRLRQQ
IPALTGNSWWEEGDGNVRWLNKNAQPLSADEWQNGPKLMQILLSDRFLIAINATLEVTDIVLPEGEWRAVPPFAGEDNPV
ITAVWQGLCVFQRG
>Mature_653_residues
TQLAIGEATPHGATYDGHGVNFTLFSAHAERVELCVFDSRGNERRYDLPGRRGDVWHGYLAGARPGLRYGYRVHGPWQPA
QGHRFNPAKLLLDPYARQVEGELKDHPLLHGGHDEPDYRDNAAVAPKSVVISDHYDWEDDAAPRTPWGKTVIYEAHVKGL
TYLHPELPQEIRGTYKALGHPVMVAYFKQLGITALELLPVAQFASEPRLQRMGLTNYWGYNPMAMFALHPAWASSPETAL
DEFRDAVKALHRAGIEVILDIVLNHSAELDLDGPTFSLRGIDNRSYYWIRDDGDYHNWTGCGNTLNLSHPGVVEYACECL
RYWMETCHVDGFRFDLASVMGRTPTFRQDAPLFAAIKACPVLSTVKLIAEPWDIGEGGYQVGNFPPPFAEWNDHFRDAAR
RFWLPRNLTTGEFACRFAASSDVFKRNGRAPGASVNLLTAHDGFTLRDCVCFNQKHNEANGEENRDGTNSNYSDNHGKEG
LGGPLDLMERRRDSIHALLATLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNALTWLDWQQANRGLTTFTAALIRLRQQI
PALTGNSWWEEGDGNVRWLNKNAQPLSADEWQNGPKLMQILLSDRFLIAINATLEVTDIVLPEGEWRAVPPFAGEDNPVI
TAVWQGLCVFQRG

Specific function: Hydrolyzes the alpha-1,6-glucosidic linkages in glycogen which has first been partially depolymerized by phosphorylase. Shows only very little activity with native glycogen [H]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=657, Percent_Identity=84.779299847793, Blast_Score=1169, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR022844
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 73254; Mature: 73123

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLAIGEATPHGATYDGHGVNFTLFSAHAERVELCVFDSRGNERRYDLPGRRGDVWHGY
CCCEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEE
LAGARPGLRYGYRVHGPWQPAQGHRFNPAKLLLDPYARQVEGELKDHPLLHGGHDEPDYR
CCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCC
DNAAVAPKSVVISDHYDWEDDAAPRTPWGKTVIYEAHVKGLTYLHPELPQEIRGTYKALG
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHC
HPVMVAYFKQLGITALELLPVAQFASEPRLQRMGLTNYWGYNPMAMFALHPAWASSPETA
CHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHH
LDEFRDAVKALHRAGIEVILDIVLNHSAELDLDGPTFSLRGIDNRSYYWIRDDGDYHNWT
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCEECCCCEEEEEECCCCEEEEEECCCCCCCCC
GCGNTLNLSHPGVVEYACECLRYWMETCHVDGFRFDLASVMGRTPTFRQDAPLFAAIKAC
CCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHH
PVLSTVKLIAEPWDIGEGGYQVGNFPPPFAEWNDHFRDAARRFWLPRNLTTGEFACRFAA
HHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHEEEECC
SSDVFKRNGRAPGASVNLLTAHDGFTLRDCVCFNQKHNEANGEENRDGTNSNYSDNHGKE
CCHHHHHCCCCCCCEEEEEEECCCCEEEHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
GLGGPLDLMERRRDSIHALLATLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNALTWLDW
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEECCCCEEEEEH
QQANRGLTTFTAALIRLRQQIPALTGNSWWEEGDGNVRWLNKNAQPLSADEWQNGPKLMQ
HHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHCCCCHHHHH
ILLSDRFLIAINATLEVTDIVLPEGEWRAVPPFAGEDNPVITAVWQGLCVFQRG
HHHCCCEEEEEECEEEEEEEEECCCCCEECCCCCCCCCCEEEEECCCEEEECCC
>Mature Secondary Structure 
TQLAIGEATPHGATYDGHGVNFTLFSAHAERVELCVFDSRGNERRYDLPGRRGDVWHGY
CCEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEE
LAGARPGLRYGYRVHGPWQPAQGHRFNPAKLLLDPYARQVEGELKDHPLLHGGHDEPDYR
CCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCC
DNAAVAPKSVVISDHYDWEDDAAPRTPWGKTVIYEAHVKGLTYLHPELPQEIRGTYKALG
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHC
HPVMVAYFKQLGITALELLPVAQFASEPRLQRMGLTNYWGYNPMAMFALHPAWASSPETA
CHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHH
LDEFRDAVKALHRAGIEVILDIVLNHSAELDLDGPTFSLRGIDNRSYYWIRDDGDYHNWT
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCEECCCCEEEEEECCCCEEEEEECCCCCCCCC
GCGNTLNLSHPGVVEYACECLRYWMETCHVDGFRFDLASVMGRTPTFRQDAPLFAAIKAC
CCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHH
PVLSTVKLIAEPWDIGEGGYQVGNFPPPFAEWNDHFRDAARRFWLPRNLTTGEFACRFAA
HHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHEEEECC
SSDVFKRNGRAPGASVNLLTAHDGFTLRDCVCFNQKHNEANGEENRDGTNSNYSDNHGKE
CCHHHHHCCCCCCCEEEEEEECCCCEEEHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
GLGGPLDLMERRRDSIHALLATLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNALTWLDW
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEECCCCEEEEEH
QQANRGLTTFTAALIRLRQQIPALTGNSWWEEGDGNVRWLNKNAQPLSADEWQNGPKLMQ
HHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHCCCCHHHHH
ILLSDRFLIAINATLEVTDIVLPEGEWRAVPPFAGEDNPVITAVWQGLCVFQRG
HHHCCCEEEEEECEEEEEEEEECCCCCEECCCCCCCCCCEEEEECCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA