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The map label for this gene is rpoH [H]

Identifier: 29144223

GI number: 29144223

Start: 4093863

End: 4094717

Strand: Direct

Name: rpoH [H]

Synonym: t3954

Alternate gene names: 29144223

Gene position: 4093863-4094717 (Clockwise)

Preceding gene: 29144222

Following gene: 29144226

Centisome position: 85.43

GC content: 54.04

Gene sequence:

>855_bases
ATGACCAAAGAAATGCAAAATTTAGCTTTAGCCCCTGTTGGCAACCTGGAATCTTATATCCGGGCGGCGAACGCGTGGCC
GATGTTATCGGCTGACGAGGAGCGGGCATTGGCTGAAAGGCTGCATTACCAGGGCGATCTGGAAGCAGCTAAAACGCTGA
TCCTGTCTCACCTGCGCTTTGTTGTTCATATTGCTCGTAACTATGCGGGCTATGGTCTGCCGCAGGCGGATTTGATCCAG
GAAGGCAACATCGGCCTGATGAAAGCCGTACGTCGTTTCAACCCGGAAGTAGGCGTGCGCCTGGTCTCCTTCGCCGTACA
CTGGATTAAAGCAGAAATTCACGAATACGTGCTGCGTAACTGGCGTATCGTTAAAGTCGCAACCACGAAAGCGCAGCGTA
AGCTGTTCTTTAATCTGCGTAAAACCAAGCAGCGTCTGGGCTGGTTTAATCAGGATGAGGTTGAAATGGTGGCGCGCGAA
CTGGGTGTTTCCAGTAAAGACGTGCGTGAGATGGAGTCGCGTATGGCGGCGCAGGACATGACGTTTGACATGTCTTCGGA
CGATGAGTCCGACAGCCAGCCAATGGCGCCGGTGCTGTATCTGCAGGATAAATCGTCTAACTTTGCCGACGGCATTGAAG
ACGATAACTGGGAAGAGCAGGCCGCTAACCGACTGACCGATGCGATGCAGGGGCTCGACGAGCGTAGCCAGGATATTATC
CGCGCTCGCTGGCTGGACGAAGACAATAAGTCTACGTTGCAGGAGCTGGCCGATCGTTACGGCGTCTCCGCTGAGCGTGT
GCGCCAGCTTGAAAAGAACGCCATGAAAAAGCTTCGCGCTGCGATCGAAGCGTAA

Upstream 100 bases:

>100_bases
CTGTCTGATAAAAGAGTGGATGATATTCTCGTTGCTCATCGGCTTTGGCACGGTTGTTGCTCGCTGACGGTGCCAGGCAA
TACTGATTGAGAGGATTTGA

Downstream 100 bases:

>100_bases
TCTTCGCGAATTGCCAATGAACCCTCGAATGTGAATTCGGGGGTTTTGTTTTTTGTAGGCCGGATAAGGCGTTTACGCTG
CTATCCGGCAACACGTTTGC

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTKEMQNLALAPVGNLESYIRAANAWPMLSADEERALAERLHYQGDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQ
EGNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
LGVSSKDVREMESRMAAQDMTFDMSSDDESDSQPMAPVLYLQDKSSNFADGIEDDNWEEQAANRLTDAMQGLDERSQDII
RARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMKKLRAAIEA

Sequences:

>Translated_284_residues
MTKEMQNLALAPVGNLESYIRAANAWPMLSADEERALAERLHYQGDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQ
EGNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
LGVSSKDVREMESRMAAQDMTFDMSSDDESDSQPMAPVLYLQDKSSNFADGIEDDNWEEQAANRLTDAMQGLDERSQDII
RARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMKKLRAAIEA
>Mature_283_residues
TKEMQNLALAPVGNLESYIRAANAWPMLSADEERALAERLHYQGDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQE
GNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVAREL
GVSSKDVREMESRMAAQDMTFDMSSDDESDSQPMAPVLYLQDKSSNFADGIEDDNWEEQAANRLTDAMQGLDERSQDIIR
ARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMKKLRAAIEA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=284, Percent_Identity=96.830985915493, Blast_Score=551, Evalue=1e-158,
Organism=Escherichia coli, GI1789098, Length=268, Percent_Identity=27.6119402985075, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789448, Length=242, Percent_Identity=28.5123966942149, Blast_Score=91, Evalue=6e-20,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32561; Mature: 32430

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKEMQNLALAPVGNLESYIRAANAWPMLSADEERALAERLHYQGDLEAAKTLILSHLRF
CCCHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRN
HHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHC
WRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARELGVSSKDVREMESRMAAQDM
CEEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
TFDMSSDDESDSQPMAPVLYLQDKSSNFADGIEDDNWEEQAANRLTDAMQGLDERSQDII
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMKKLRAAIEA
HHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKEMQNLALAPVGNLESYIRAANAWPMLSADEERALAERLHYQGDLEAAKTLILSHLRF
CCHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRN
HHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHC
WRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARELGVSSKDVREMESRMAAQDM
CEEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
TFDMSSDDESDSQPMAPVLYLQDKSSNFADGIEDDNWEEQAANRLTDAMQGLDERSQDII
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMKKLRAAIEA
HHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]