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The map label for this gene is fruA [H]

Identifier: 29143959

GI number: 29143959

Start: 3773795

End: 3775168

Strand: Direct

Name: fruA [H]

Synonym: t3665

Alternate gene names: 29143959

Gene position: 3773795-3775168 (Clockwise)

Preceding gene: 29143958

Following gene: 29143960

Centisome position: 78.75

GC content: 56.11

Gene sequence:

>1374_bases
ATGACTAAAAAAATTGTTGCTGTGACGGCCTGTCCGACGGGCGTCGCCCATGCCTTTATGGCTGCCGAAGCGCTGGAAAT
TGAAGCCCGCAAGCGTGGTGACTGGATCAAGGTTGAAACCCGCGGTTCCGTCGGGGCGAAGAACACGCTGACCGCGGAAG
AGATAGCGCAAGCGGATGTGGTGATTATCGCGGCGGATATTGAGCTGGATCTGTCAGGCTTTGTTGGCAAAAGACTCTAT
CGCACCAGTACCGGCGCGGCGCTAAAAAAGAGTGCGCAGGAAATGGATAATGCTTTCAATAGCGCCGAGGTCTATCAGGG
GAGTGCCGGGCGCTCTTCTTCAGCTAGTAAAACAGAGCTACCCGGCGTTTATAAGCATTTAATGACCGGCGTATCCCATA
TGCTTCCTTTGGTGGTGGCGGGAGGGTTATGTATCGCGCTCTCTTTTGTCTTCGGTATCCAGGCCTTCAATGAGCCGGGC
ACGCTGGCGGCTGCGTTATTTCAAATCGGTGGTAAGGCGGCCTTTGCGCTGATGGTGCCGGTACTGGCTGGGTTTATTGC
CTTCTCAATTGCCGATCGTCCGGGTCTGGCCCCGGGGCTTATTGGCGGGATGTTGGCCAGCCTGTGCGGTGCTGGTTTCC
TGGGAGGGATTGTGGCCGGTTTTCTGGCCGGTTACAGCGTACGGTTTCTGGCGCAAAATATTAAGCTGCCAGCGTCAATG
GAAGCGCTTAAGCCGGTACTGGTTCTGCCGCTACTGTCTACGCTGATTACCGGCCTAATCATGATTTACGTGGTGGGTGG
CCCCGTTTCTGCGGTGATGGAAGGGCTGACGACGTTCCTGGGGAATATGACCTCTACGAATGCTATCTTGCTGGGAATGT
TGCTGGGCGCAATGCAAGGTTTTGACCTTGGTGGGCCGGTGAATAAAGCTGCGTATACCTTCGGCGTCGGTTTACTGGCT
TCTCATTCCTATATGCCGATGGCGGCCATTATGGCGGCAGGGATGGTACCGGCATTAGGGATGGGCGTCGCAACCTGGGC
GGCCAGAGCGAAATTCACTGCCTCTGAGCATGAAGCGGGTAACGCATCCTTTATCCTGGGGCTGTGCTTTATCTCTGAAG
GGGCGATTCCCTTCGCTGCGCGTGACCCGATGCGCGTCATCCCTTCCACGATGGTCGGTGGCGCAATCGCCGGGGGACTG
TCCATGTATTTCGGTTGTACACTGATGGCGCCACACGGTGGTCTCTTTGTCCTGGCGATCCCGCATGCGGTAGAACATGT
GATGCAGTACCTGCTCTCGATTGCCCTGGGCACGATTGTCTGCGGCCTGATGTACGCGCTGTTGAAACCGTCTGCGGTTG
CGCAAACAGTTTAA

Upstream 100 bases:

>100_bases
TCGCCCGGATGACGCACTGTTCCAGAAAGCCGCGGCCTGGTCACGTCTGTAACATTTTTTCCCGCTTTTTCTTTAAATTA
AAAGAACAGGGTATCCTACT

Downstream 100 bases:

>100_bases
TTCATCTCCCCCTGCAGGCTGGTCTGGTGTCTCCCCGGGCCAGCTTTTTTATTTCCATTGTCATCAAACCGTCATAATCA
AGACATATAACTGTCATCAA

Product: PTS system permease

Products: NA

Alternate protein names: EIIBC-Fru; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MTKKIVAVTACPTGVAHAFMAAEALEIEARKRGDWIKVETRGSVGAKNTLTAEEIAQADVVIIAADIELDLSGFVGKRLY
RTSTGAALKKSAQEMDNAFNSAEVYQGSAGRSSSASKTELPGVYKHLMTGVSHMLPLVVAGGLCIALSFVFGIQAFNEPG
TLAAALFQIGGKAAFALMVPVLAGFIAFSIADRPGLAPGLIGGMLASLCGAGFLGGIVAGFLAGYSVRFLAQNIKLPASM
EALKPVLVLPLLSTLITGLIMIYVVGGPVSAVMEGLTTFLGNMTSTNAILLGMLLGAMQGFDLGGPVNKAAYTFGVGLLA
SHSYMPMAAIMAAGMVPALGMGVATWAARAKFTASEHEAGNASFILGLCFISEGAIPFAARDPMRVIPSTMVGGAIAGGL
SMYFGCTLMAPHGGLFVLAIPHAVEHVMQYLLSIALGTIVCGLMYALLKPSAVAQTV

Sequences:

>Translated_457_residues
MTKKIVAVTACPTGVAHAFMAAEALEIEARKRGDWIKVETRGSVGAKNTLTAEEIAQADVVIIAADIELDLSGFVGKRLY
RTSTGAALKKSAQEMDNAFNSAEVYQGSAGRSSSASKTELPGVYKHLMTGVSHMLPLVVAGGLCIALSFVFGIQAFNEPG
TLAAALFQIGGKAAFALMVPVLAGFIAFSIADRPGLAPGLIGGMLASLCGAGFLGGIVAGFLAGYSVRFLAQNIKLPASM
EALKPVLVLPLLSTLITGLIMIYVVGGPVSAVMEGLTTFLGNMTSTNAILLGMLLGAMQGFDLGGPVNKAAYTFGVGLLA
SHSYMPMAAIMAAGMVPALGMGVATWAARAKFTASEHEAGNASFILGLCFISEGAIPFAARDPMRVIPSTMVGGAIAGGL
SMYFGCTLMAPHGGLFVLAIPHAVEHVMQYLLSIALGTIVCGLMYALLKPSAVAQTV
>Mature_456_residues
TKKIVAVTACPTGVAHAFMAAEALEIEARKRGDWIKVETRGSVGAKNTLTAEEIAQADVVIIAADIELDLSGFVGKRLYR
TSTGAALKKSAQEMDNAFNSAEVYQGSAGRSSSASKTELPGVYKHLMTGVSHMLPLVVAGGLCIALSFVFGIQAFNEPGT
LAAALFQIGGKAAFALMVPVLAGFIAFSIADRPGLAPGLIGGMLASLCGAGFLGGIVAGFLAGYSVRFLAQNIKLPASME
ALKPVLVLPLLSTLITGLIMIYVVGGPVSAVMEGLTTFLGNMTSTNAILLGMLLGAMQGFDLGGPVNKAAYTFGVGLLAS
HSYMPMAAIMAAGMVPALGMGVATWAARAKFTASEHEAGNASFILGLCFISEGAIPFAARDPMRVIPSTMVGGAIAGGLS
MYFGCTLMAPHGGLFVLAIPHAVEHVMQYLLSIALGTIVCGLMYALLKPSAVAQTV

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=449, Percent_Identity=66.1469933184855, Blast_Score=600, Evalue=1e-173,
Organism=Escherichia coli, GI1786951, Length=433, Percent_Identity=38.1062355658199, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI87082348, Length=430, Percent_Identity=35.1162790697674, Blast_Score=241, Evalue=6e-65,
Organism=Escherichia coli, GI1790386, Length=318, Percent_Identity=41.1949685534591, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1788729, Length=406, Percent_Identity=28.8177339901478, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI1790387, Length=89, Percent_Identity=51.685393258427, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788730, Length=94, Percent_Identity=42.5531914893617, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 46916; Mature: 46785

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKIVAVTACPTGVAHAFMAAEALEIEARKRGDWIKVETRGSVGAKNTLTAEEIAQADV
CCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHCCCCE
VIIAADIELDLSGFVGKRLYRTSTGAALKKSAQEMDNAFNSAEVYQGSAGRSSSASKTEL
EEEEEECEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCC
PGVYKHLMTGVSHMLPLVVAGGLCIALSFVFGIQAFNEPGTLAAALFQIGGKAAFALMVP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHH
VLAGFIAFSIADRPGLAPGLIGGMLASLCGAGFLGGIVAGFLAGYSVRFLAQNIKLPASM
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
EALKPVLVLPLLSTLITGLIMIYVVGGPVSAVMEGLTTFLGNMTSTNAILLGMLLGAMQG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
FDLGGPVNKAAYTFGVGLLASHSYMPMAAIMAAGMVPALGMGVATWAARAKFTASEHEAG
CCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
NASFILGLCFISEGAIPFAARDPMRVIPSTMVGGAIAGGLSMYFGCTLMAPHGGLFVLAI
CHHHEEHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCEEEECCCCCEEEEEC
PHAVEHVMQYLLSIALGTIVCGLMYALLKPSAVAQTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
>Mature Secondary Structure 
TKKIVAVTACPTGVAHAFMAAEALEIEARKRGDWIKVETRGSVGAKNTLTAEEIAQADV
CCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHCCCCE
VIIAADIELDLSGFVGKRLYRTSTGAALKKSAQEMDNAFNSAEVYQGSAGRSSSASKTEL
EEEEEECEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCC
PGVYKHLMTGVSHMLPLVVAGGLCIALSFVFGIQAFNEPGTLAAALFQIGGKAAFALMVP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHH
VLAGFIAFSIADRPGLAPGLIGGMLASLCGAGFLGGIVAGFLAGYSVRFLAQNIKLPASM
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
EALKPVLVLPLLSTLITGLIMIYVVGGPVSAVMEGLTTFLGNMTSTNAILLGMLLGAMQG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
FDLGGPVNKAAYTFGVGLLASHSYMPMAAIMAAGMVPALGMGVATWAARAKFTASEHEAG
CCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
NASFILGLCFISEGAIPFAARDPMRVIPSTMVGGAIAGGLSMYFGCTLMAPHGGLFVLAI
CHHHEEHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCEEEECCCCCEEEEEC
PHAVEHVMQYLLSIALGTIVCGLMYALLKPSAVAQTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3076173; 9278503; 9097040; 1981619 [H]