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The map label for this gene is aroE [C]

Identifier: 29143958

GI number: 29143958

Start: 3772976

End: 3773746

Strand: Direct

Name: aroE [C]

Synonym: t3664

Alternate gene names: 29143958

Gene position: 3772976-3773746 (Clockwise)

Preceding gene: 29143956

Following gene: 29143959

Centisome position: 78.74

GC content: 53.31

Gene sequence:

>771_bases
ATGACAAAGCGCATTAATAAAGACACTCAGGTCTGCATGTCCCTGGCTGCACGCCCAAGTAACTTCGGTACACGCTTTCA
CAACTATCTGTACGAAGCGCTGGATCTCAATTATCTCTACAAAGCTTTCTCGCCGACCGATCTTACGCAGGCCATTAGCG
GCGTGCGCGGGCTGGGGATACGTGGGTGCGCGATCTCTATGCCATTTAAGGAAGCCTGCATTCCGCTGGTGGATGAACTG
GATGCTTCCGCTAAGGCGTACAACACCGATTACATTGCAATAGCTCGCTTGCTGGCGAATTACAAGGTCTCTCCCGACCA
GGTGTTTGCTTTGCGTGGCAGCGGTGGTATGGCAAAGGCGGTGGCCTGCGCGCTGAAGGATGCGGGGTTTACCCGTGGTT
ATATTGTCGCGATTGATGAAGCGTCAGGTAAACAACTGGCCGAACTCTATGGCTATGAATGGCGCCCGGATACCGACGGT
ATTGAAGCCGATTTGTTAATTAACGCCACGCCTATCGGAATGGCTGGCGGTAATGATGCCGACAAACTCTCTTATTCCGA
ACAAGAAGTGGATAAGGCGAGCGTTATCTTTGATGTGGTGGCGCTACCGCCCGTCACGCCGCTAATCCGCTACGCCCGTG
AACGCGGTAAAACCGTGATTATCGGTTCCGAAGTGTTTGCTATTCAGGCCGTGGAGCAATTTGTCCTTTATACCGGGATT
CGCCCGGATGACGCACTGTTCCAGAAAGCCGCGGCCTGGTCACGTCTGTAA

Upstream 100 bases:

>100_bases
TGCCATAAAAGATGAAATTTCCCGAACGGGAATGAGCAATTTTTAATATCCCCTTTTTAGCCGATGATTTTCTCTATCGC
AACTTCTATGAGGATTCATC

Downstream 100 bases:

>100_bases
CATTTTTTCCCGCTTTTTCTTTAAATTAAAAGAACAGGGTATCCTACTATGACTAAAAAAATTGTTGCTGTGACGGCCTG
TCCGACGGGCGTCGCCCATG

Product: shikimate 5-dehydrogenase

Products: 3-dehydroshikimate; NADPH; H+

Alternate protein names: SDH-L [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTKRINKDTQVCMSLAARPSNFGTRFHNYLYEALDLNYLYKAFSPTDLTQAISGVRGLGIRGCAISMPFKEACIPLVDEL
DASAKAYNTDYIAIARLLANYKVSPDQVFALRGSGGMAKAVACALKDAGFTRGYIVAIDEASGKQLAELYGYEWRPDTDG
IEADLLINATPIGMAGGNDADKLSYSEQEVDKASVIFDVVALPPVTPLIRYARERGKTVIIGSEVFAIQAVEQFVLYTGI
RPDDALFQKAAAWSRL

Sequences:

>Translated_256_residues
MTKRINKDTQVCMSLAARPSNFGTRFHNYLYEALDLNYLYKAFSPTDLTQAISGVRGLGIRGCAISMPFKEACIPLVDEL
DASAKAYNTDYIAIARLLANYKVSPDQVFALRGSGGMAKAVACALKDAGFTRGYIVAIDEASGKQLAELYGYEWRPDTDG
IEADLLINATPIGMAGGNDADKLSYSEQEVDKASVIFDVVALPPVTPLIRYARERGKTVIIGSEVFAIQAVEQFVLYTGI
RPDDALFQKAAAWSRL
>Mature_255_residues
TKRINKDTQVCMSLAARPSNFGTRFHNYLYEALDLNYLYKAFSPTDLTQAISGVRGLGIRGCAISMPFKEACIPLVDELD
ASAKAYNTDYIAIARLLANYKVSPDQVFALRGSGGMAKAVACALKDAGFTRGYIVAIDEASGKQLAELYGYEWRPDTDGI
EADLLINATPIGMAGGNDADKLSYSEQEVDKASVIFDVVALPPVTPLIRYARERGKTVIIGSEVFAIQAVEQFVLYTGIR
PDDALFQKAAAWSRL

Specific function: The physiological substrate is not known. Has much lower activity towards shikimate than AroE [H]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR013708
- InterPro:   IPR006151 [H]

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N [H]

EC number: 1.1.1.25

Molecular weight: Translated: 27899; Mature: 27768

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRINKDTQVCMSLAARPSNFGTRFHNYLYEALDLNYLYKAFSPTDLTQAISGVRGLGI
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
RGCAISMPFKEACIPLVDELDASAKAYNTDYIAIARLLANYKVSPDQVFALRGSGGMAKA
CCEEEECCHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHH
VACALKDAGFTRGYIVAIDEASGKQLAELYGYEWRPDTDGIEADLLINATPIGMAGGNDA
HHHHHHHCCCCCCEEEEEECCCCHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCH
DKLSYSEQEVDKASVIFDVVALPPVTPLIRYARERGKTVIIGSEVFAIQAVEQFVLYTGI
HHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHCCC
RPDDALFQKAAAWSRL
CCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TKRINKDTQVCMSLAARPSNFGTRFHNYLYEALDLNYLYKAFSPTDLTQAISGVRGLGI
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
RGCAISMPFKEACIPLVDELDASAKAYNTDYIAIARLLANYKVSPDQVFALRGSGGMAKA
CCEEEECCHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHH
VACALKDAGFTRGYIVAIDEASGKQLAELYGYEWRPDTDGIEADLLINATPIGMAGGNDA
HHHHHHHCCCCCCEEEEEECCCCHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCH
DKLSYSEQEVDKASVIFDVVALPPVTPLIRYARERGKTVIIGSEVFAIQAVEQFVLYTGI
HHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHCCC
RPDDALFQKAAAWSRL
CCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: shikimate; NADP+

Specific reaction: shikimate + NADP+ = 3-dehydroshikimate + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]