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The map label for this gene is fdoH [H]

Identifier: 29143885

GI number: 29143885

Start: 3686254

End: 3687156

Strand: Direct

Name: fdoH [H]

Synonym: t3584

Alternate gene names: 29143885

Gene position: 3686254-3687156 (Clockwise)

Preceding gene: 29143884

Following gene: 29143886

Centisome position: 76.93

GC content: 58.14

Gene sequence:

>903_bases
ATGGCTTATCAATCGCAAGACATCATTCGTCGTTCCGCCACTAACGGTTTCACCCCCGCGCCCCAGGCGCGGGACCACCA
GGAAGAGGTGGCGAAACTCATCGACGTCACCACCTGTATCGGCTGTAAAGCGTGTCAGGTGGCGTGCTCCGAGTGGAACG
ACATCCGTGATGAAGTGGGCAATAACGTCGGGGTGTATGACAACCCGGCGGACTTAACTGCGAAATCCTGGACGGTGATG
CGCTTCTCGGAAGTGGAACAGAACGACAAACTGGAGTGGCTGATCCGTAAGGATGGCTGTATGCACTGCGCCGATCCCGG
CTGCCTGAAGGCCTGTCCGGCGGAAGGGGCGATCATTCAGTACGCTAACGGTATTGTCGACTTCCAGTCCGAGCAGTGTA
TCGGCTGCGGCTACTGTATCGCTGGCTGCCCGTTCAACGTGCCGCGCCTCAACCCGGAAGACAACCGCGTCTATAAATGT
ACGCTGTGCGTTGACTGTGTGGTGGTCGGCCAGGAACCGGCCTGCGTGAAGACCTGTCCAACCGGCGCTATTCATTTTGG
CTCGAAAGAGGATATGAAAACGCTGGCGGGCGAGCGGGTGGCGGAACTCAAAACCCGTGGTTATGACAATGCGGGTCTGT
ACGATCCGGCGGGCGTAGGCGGTACGCATGTCATGTACGTGCTGCATCATGCCGACAAGCCGAATCTGTATCACGGTCTG
CCGGAGAACCCGGAAATCAGCGAAACCGTTAAGTTCTGGAAAGGCGTCTGGAAACCGCTCGCCGCGTTCGGTTTTGCCGC
GACCTTTGCCGCCAGCGTCTTCCACTATGTCGGCGTCGGCCCGAACCGCGCGGAAGAGGAAGATGACAACCTGCATGAAG
AGAAAGACGAGGTGCGCAAATGA

Upstream 100 bases:

>100_bases
AGCCAATACGTTAACGCCTTTCGTCGGCGATGCGAACACGCAAACGCCGGAGTTTAAATCCTTCCTTGTGAACGTGGAAA
AGGTGTAACGGAGACGACCT

Downstream 100 bases:

>100_bases
AACGACGTGACACCATCGTGCGCTATACGGCGCCGGAACGCATCAACCACTGGATCGTCGCCTTCTGCTTCGTGCTGGCG
GCGGTGAGTGGGCTGGGCTT

Product: formate dehydrogenase-O beta subunit

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase iron-sulfur subunit; FDH-Z subunit beta; Formate dehydrogenase-O subunit beta [H]

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MAYQSQDIIRRSATNGFTPAPQARDHQEEVAKLIDVTTCIGCKACQVACSEWNDIRDEVGNNVGVYDNPADLTAKSWTVM
RFSEVEQNDKLEWLIRKDGCMHCADPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFNVPRLNPEDNRVYKC
TLCVDCVVVGQEPACVKTCPTGAIHFGSKEDMKTLAGERVAELKTRGYDNAGLYDPAGVGGTHVMYVLHHADKPNLYHGL
PENPEISETVKFWKGVWKPLAAFGFAATFAASVFHYVGVGPNRAEEEDDNLHEEKDEVRK

Sequences:

>Translated_300_residues
MAYQSQDIIRRSATNGFTPAPQARDHQEEVAKLIDVTTCIGCKACQVACSEWNDIRDEVGNNVGVYDNPADLTAKSWTVM
RFSEVEQNDKLEWLIRKDGCMHCADPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFNVPRLNPEDNRVYKC
TLCVDCVVVGQEPACVKTCPTGAIHFGSKEDMKTLAGERVAELKTRGYDNAGLYDPAGVGGTHVMYVLHHADKPNLYHGL
PENPEISETVKFWKGVWKPLAAFGFAATFAASVFHYVGVGPNRAEEEDDNLHEEKDEVRK
>Mature_299_residues
AYQSQDIIRRSATNGFTPAPQARDHQEEVAKLIDVTTCIGCKACQVACSEWNDIRDEVGNNVGVYDNPADLTAKSWTVMR
FSEVEQNDKLEWLIRKDGCMHCADPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFNVPRLNPEDNRVYKCT
LCVDCVVVGQEPACVKTCPTGAIHFGSKEDMKTLAGERVAELKTRGYDNAGLYDPAGVGGTHVMYVLHHADKPNLYHGLP
ENPEISETVKFWKGVWKPLAAFGFAATFAASVFHYVGVGPNRAEEEDDNLHEEKDEVRK

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790326, Length=300, Percent_Identity=93.3333333333333, Blast_Score=594, Evalue=1e-171,
Organism=Escherichia coli, GI1787749, Length=294, Percent_Identity=76.8707482993197, Blast_Score=489, Evalue=1e-140,
Organism=Escherichia coli, GI1789370, Length=184, Percent_Identity=32.0652173913043, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787872, Length=175, Percent_Identity=32, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787122, Length=169, Percent_Identity=31.3609467455621, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI2367345, Length=178, Percent_Identity=30.3370786516854, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI226510944, Length=192, Percent_Identity=29.1666666666667, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787963, Length=154, Percent_Identity=24.6753246753247, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082179, Length=172, Percent_Identity=26.7441860465116, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 33000; Mature: 32869

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
5.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYQSQDIIRRSATNGFTPAPQARDHQEEVAKLIDVTTCIGCKACQVACSEWNDIRDEVG
CCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHC
NNVGVYDNPADLTAKSWTVMRFSEVEQNDKLEWLIRKDGCMHCADPGCLKACPAEGAIIQ
CCCCCCCCCCCCCCCCEEEEEEHHCCCCCCEEEEEECCCCCCCCCCCHHHCCCCCCCEEE
YANGIVDFQSEQCIGCGYCIAGCPFNVPRLNPEDNRVYKCTLCVDCVVVGQEPACVKTCP
ECCCEEECCCCCCCCCCCEECCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCEEEECC
TGAIHFGSKEDMKTLAGERVAELKTRGYDNAGLYDPAGVGGTHVMYVLHHADKPNLYHGL
CCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCC
PENPEISETVKFWKGVWKPLAAFGFAATFAASVFHYVGVGPNRAEEEDDNLHEEKDEVRK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
AYQSQDIIRRSATNGFTPAPQARDHQEEVAKLIDVTTCIGCKACQVACSEWNDIRDEVG
CCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHC
NNVGVYDNPADLTAKSWTVMRFSEVEQNDKLEWLIRKDGCMHCADPGCLKACPAEGAIIQ
CCCCCCCCCCCCCCCCEEEEEEHHCCCCCCEEEEEECCCCCCCCCCCHHHCCCCCCCEEE
YANGIVDFQSEQCIGCGYCIAGCPFNVPRLNPEDNRVYKCTLCVDCVVVGQEPACVKTCP
ECCCEEECCCCCCCCCCCEECCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCEEEECC
TGAIHFGSKEDMKTLAGERVAELKTRGYDNAGLYDPAGVGGTHVMYVLHHADKPNLYHGL
CCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCC
PENPEISETVKFWKGVWKPLAAFGFAATFAASVFHYVGVGPNRAEEEDDNLHEEKDEVRK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]