Click here to switch to the map view.

The map label for this gene is thiH [H]

Identifier: 29143786

GI number: 29143786

Start: 3560038

End: 3561171

Strand: Direct

Name: thiH [H]

Synonym: t3472

Alternate gene names: 29143786

Gene position: 3560038-3561171 (Clockwise)

Preceding gene: 29143785

Following gene: 29143796

Centisome position: 74.29

GC content: 55.11

Gene sequence:

>1134_bases
ATGAAAGCCTTCACCGACCGTTGGCGGCAACTGGAGTGGGACGATATTCGCCTGCGCATCAACGGTAAAACCGCCGCCGA
TGTGGAGCGGGCGCTGAATGCTTCACGCCTCAACCGCGAGGATATGATGGCGTTGCTCTCCCCCGCCGCCGCCGATTATC
TGGAACCGATGGCGCAGCGGGCACAAAGGCTGACCCGACAACGCTTTGGCAACACCGTCAGTTTCTACGTGCCGCTCTAT
CTCTCAAACCTCTGTGCCAACGACTGCACCTACTGCGGCTTCTCTATGAGCAACCGCATCAAGCGTAAGACGCTGGATGA
GGTGGATATTCAAAGGGAGTGCGATGCTATCCGTAAACTGGGTTTTGAGCATCTGCTGTTAGTCACCGGCGAGCATCAGG
CCAAAGTCGGCATGGACTATTTTCGCCGTCATTTACCCACCATCCGCCGTCAATTTTCCTCTTTACAGATGGAAGTCCAG
CCCTTGTCGCAAGAAAACTACGCGGAGCTCAAAACGCTGGGGATCGATGGCGTGATGGTTTATCAGGAAACTTACCATGA
GGCAATCTATGCACAGCATCACCTGAAGGGAAAGAAACAGGACTTTTTCTGGCGGCTGGAAACGCCGGATCGGTTAGGCC
GGGCAGGTATCGACAAAATCGGTCTTGGCGCGCTAATTGGTCTGTCGGACAACTGGCGGGTGGATTGCTATATGGTGGCG
GAGCATCTGTTGTGGATGCAGAAACACTATTGGCAGAGTCGCTATTCTGTTTCCTTCCCGCGTCTGCGTCCGTGTACCGG
CGGTGTGGAACCCGCATCTGTGATGGATGAAAAGCAACTGGTGCAAACGATTTGCGCGTTTCGTTTACTGGCGCCAGAAA
TTGAATTATCACTCTCCACCCGCGAATCGCCGTGGTTTCGAGACCATGTGATTCCTCTGGCAATCAACAACGTCAGCGCC
TTCTCGAAAACCCAGCCCGGTGGCTACGCTGACGATCATCCGGAACTGGAGCAGTTTTCTCCCCACGATGACCGTCGGCC
AGAAGTCGTTGCAAGCGCGTTAAGCGCGCAAGGGTTACAGCCCGTATGGAAAGACTGGGACAGTTGGCTGGGGCGCGCTT
CGCAAATGCGGTGA

Upstream 100 bases:

>100_bases
CGGTGGAAGCCGGATTACTGGCGCGTCAGGCGGTGCCGGGTAATCGTAGCACCTACGCCAGCGCTACCAGCCCGCTCACC
GGATTTCTGGAGGCGTTAGC

Downstream 100 bases:

>100_bases
ACCCGTATGTAAACGCATTAATATTTCACGAGCGAGTACGTAATGTGAAAAACGGGAGATTGTTATCGGCCTCCCGTTTT
TTATTTTGTCATCGTCAGCA

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MKAFTDRWRQLEWDDIRLRINGKTAADVERALNASRLNREDMMALLSPAAADYLEPMAQRAQRLTRQRFGNTVSFYVPLY
LSNLCANDCTYCGFSMSNRIKRKTLDEVDIQRECDAIRKLGFEHLLLVTGEHQAKVGMDYFRRHLPTIRRQFSSLQMEVQ
PLSQENYAELKTLGIDGVMVYQETYHEAIYAQHHLKGKKQDFFWRLETPDRLGRAGIDKIGLGALIGLSDNWRVDCYMVA
EHLLWMQKHYWQSRYSVSFPRLRPCTGGVEPASVMDEKQLVQTICAFRLLAPEIELSLSTRESPWFRDHVIPLAINNVSA
FSKTQPGGYADDHPELEQFSPHDDRRPEVVASALSAQGLQPVWKDWDSWLGRASQMR

Sequences:

>Translated_377_residues
MKAFTDRWRQLEWDDIRLRINGKTAADVERALNASRLNREDMMALLSPAAADYLEPMAQRAQRLTRQRFGNTVSFYVPLY
LSNLCANDCTYCGFSMSNRIKRKTLDEVDIQRECDAIRKLGFEHLLLVTGEHQAKVGMDYFRRHLPTIRRQFSSLQMEVQ
PLSQENYAELKTLGIDGVMVYQETYHEAIYAQHHLKGKKQDFFWRLETPDRLGRAGIDKIGLGALIGLSDNWRVDCYMVA
EHLLWMQKHYWQSRYSVSFPRLRPCTGGVEPASVMDEKQLVQTICAFRLLAPEIELSLSTRESPWFRDHVIPLAINNVSA
FSKTQPGGYADDHPELEQFSPHDDRRPEVVASALSAQGLQPVWKDWDSWLGRASQMR
>Mature_377_residues
MKAFTDRWRQLEWDDIRLRINGKTAADVERALNASRLNREDMMALLSPAAADYLEPMAQRAQRLTRQRFGNTVSFYVPLY
LSNLCANDCTYCGFSMSNRIKRKTLDEVDIQRECDAIRKLGFEHLLLVTGEHQAKVGMDYFRRHLPTIRRQFSSLQMEVQ
PLSQENYAELKTLGIDGVMVYQETYHEAIYAQHHLKGKKQDFFWRLETPDRLGRAGIDKIGLGALIGLSDNWRVDCYMVA
EHLLWMQKHYWQSRYSVSFPRLRPCTGGVEPASVMDEKQLVQTICAFRLLAPEIELSLSTRESPWFRDHVIPLAINNVSA
FSKTQPGGYADDHPELEQFSPHDDRRPEVVASALSAQGLQPVWKDWDSWLGRASQMR

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=375, Percent_Identity=89.6, Blast_Score=717, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 43602; Mature: 43602

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFTDRWRQLEWDDIRLRINGKTAADVERALNASRLNREDMMALLSPAAADYLEPMAQR
CCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKRKTLDEVDIQRECDAIRKL
HHHHHHHHHCCEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GFEHLLLVTGEHQAKVGMDYFRRHLPTIRRQFSSLQMEVQPLSQENYAELKTLGIDGVMV
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHCCCCEEEE
YQETYHEAIYAQHHLKGKKQDFFWRLETPDRLGRAGIDKIGLGALIGLSDNWRVDCYMVA
HHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHCCCCHHCCCEEEECCCCCCCHHHHHH
EHLLWMQKHYWQSRYSVSFPRLRPCTGGVEPASVMDEKQLVQTICAFRLLAPEIELSLST
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
RESPWFRDHVIPLAINNVSAFSKTQPGGYADDHPELEQFSPHDDRRPEVVASALSAQGLQ
CCCCCHHHCEEEEEECCCHHHCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCC
PVWKDWDSWLGRASQMR
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKAFTDRWRQLEWDDIRLRINGKTAADVERALNASRLNREDMMALLSPAAADYLEPMAQR
CCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKRKTLDEVDIQRECDAIRKL
HHHHHHHHHCCEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GFEHLLLVTGEHQAKVGMDYFRRHLPTIRRQFSSLQMEVQPLSQENYAELKTLGIDGVMV
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHCCCCEEEE
YQETYHEAIYAQHHLKGKKQDFFWRLETPDRLGRAGIDKIGLGALIGLSDNWRVDCYMVA
HHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHCCCCHHCCCEEEECCCCCCCHHHHHH
EHLLWMQKHYWQSRYSVSFPRLRPCTGGVEPASVMDEKQLVQTICAFRLLAPEIELSLST
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
RESPWFRDHVIPLAINNVSAFSKTQPGGYADDHPELEQFSPHDDRRPEVVASALSAQGLQ
CCCCCHHHCEEEEEECCCHHHCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCC
PVWKDWDSWLGRASQMR
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]