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The map label for this gene is hydH

Identifier: 29143772

GI number: 29143772

Start: 3548360

End: 3549757

Strand: Reverse

Name: hydH

Synonym: t3458

Alternate gene names: 29143772

Gene position: 3549757-3548360 (Counterclockwise)

Preceding gene: 29143774

Following gene: 29143771

Centisome position: 74.08

GC content: 54.43

Gene sequence:

>1398_bases
GTGAGTTTTATACGATTACATAAAGATGCCGCTGCAATGTGGTTGAGTCGGCTGTTACCTGCGGCGATCTTCATTCTGGT
GGGGCTATTTTCCATTATGGTCATTCGCGACTATGGCCGTGAGAGCGCTGCCGCCAGGCAGACGCTGCTGGAAAAGGGAA
ATGTACTCATTCGCGCTCTGGAGTCCGGCACGCGAGTAGGTATGGGAATGCGGATGCATCACGCGCAGCAGCAGACATTG
CTGGAAGAGATGGCGGGTCAGCCAGGCGTGTTGTGGTTCGCTGTAACGGATGCACAGGGGGTAATCATCACTCATAGCAA
CCCTGGCATGGTGGGCAAGTCGCTCTATTCCCCATCTGAAATGCATCAACTAAACCCCGGGCCGCAGGAGTGTTGGCGGC
GTGTCGACGTTGCTGCTAACGGCGAGACGGTGCCTGCGCTGGAAATTTACCGTCAGTTCCAGCCGCTTTTCGGTATGAGA
GGTCACGGTATGAGGGGGCACGGCATGGCGCGTAGCGCCAATGACGACGAACCAGCGAAACAAACTATTTTTATTGCCTT
TGACGCCAGCGAGCTGGCCGCGACTCAGGCAAGGGAGTGGCGTAATACGCTGATTGTTTTGTCTGCGTTAGCGGCGGTGC
TGTTGGCAACGTTACTGGCGTTTTTTTGGTATCAGCGTTATCAGCGCTCGCACAGAGAACTGCTTGATGCAATGAAGCGT
AAAGAAAAGTTGGTGGCGATGGGGCATCTGGCGGCGGGCGTCGCGCACGAAATTCGTAACCCGCTCTCGTCCATCAAAGG
GCTGGCGAAATACTTTGCCGAGCGCACGCCTGCCGGCGGCGAGTCGCATGAACTGGCGCAGGTGATGGCCAAAGAGGCCG
ACCGTTTGAACCGGGTGGTAAGCGAATTGCTCGAACTGGTAAAGCCCGCGCATCTGACGCTCCAGGCGGTTAACCTCAAT
GATATTATTACTCACTCCCTGAATCTGGTCAGTCAGGATGCCCAAAGCAGAGAGATTCAGTTGAGATTCACGGCGAATGA
GACGCTTAAGCGTATTCAGGCTGACCCGGACAGGCTGACTCAGGTGCTGTTGAATTTGTACCTGAACGCTATTCATGCGA
TTGGCCGCCAGGGAACGATTACCGTAGAGGCAAAAGAAAGTGGGACCGACCGCGTTATCATCACTGTTACCGATAGCGGG
AAAGGGATTGCCCCGGATCAGCTGGAGGCGATTTTTACACCTTATTTTACGACCAAAGCTGACGGGACAGGCCTGGGGCT
GGCGGTGGTGCAAAATATCATCGAACAGCATGGCGGCGCGATTAAGGTGAAAAGTATTGAGGGTAAAGGCGCGGTCTTTA
CTATCTGGTTGCCAGTGATAGCGAGACAACAGGATTAA

Upstream 100 bases:

>100_bases
CCGTATCCGAAAGGGTGTGCGACAACGCGCGGTTATGCGGAGCGAAGAATGGCATGATCTCTGCTTAAGTAAGGAAAATA
AAAACGAGCCGGGGAAGCCT

Downstream 100 bases:

>100_bases
CAAGGGTGATACGCGGAAAAATCGATATTCTGGTTGTGGATGATGATGTAAGCCACTGCACGATTTTACAGGCGCTGCTT
CGGGGCTGGGGCTATAACGT

Product: sensor protein ZraS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MSFIRLHKDAAAMWLSRLLPAAIFILVGLFSIMVIRDYGRESAAARQTLLEKGNVLIRALESGTRVGMGMRMHHAQQQTL
LEEMAGQPGVLWFAVTDAQGVIITHSNPGMVGKSLYSPSEMHQLNPGPQECWRRVDVAANGETVPALEIYRQFQPLFGMR
GHGMRGHGMARSANDDEPAKQTIFIAFDASELAATQAREWRNTLIVLSALAAVLLATLLAFFWYQRYQRSHRELLDAMKR
KEKLVAMGHLAAGVAHEIRNPLSSIKGLAKYFAERTPAGGESHELAQVMAKEADRLNRVVSELLELVKPAHLTLQAVNLN
DIITHSLNLVSQDAQSREIQLRFTANETLKRIQADPDRLTQVLLNLYLNAIHAIGRQGTITVEAKESGTDRVIITVTDSG
KGIAPDQLEAIFTPYFTTKADGTGLGLAVVQNIIEQHGGAIKVKSIEGKGAVFTIWLPVIARQQD

Sequences:

>Translated_465_residues
MSFIRLHKDAAAMWLSRLLPAAIFILVGLFSIMVIRDYGRESAAARQTLLEKGNVLIRALESGTRVGMGMRMHHAQQQTL
LEEMAGQPGVLWFAVTDAQGVIITHSNPGMVGKSLYSPSEMHQLNPGPQECWRRVDVAANGETVPALEIYRQFQPLFGMR
GHGMRGHGMARSANDDEPAKQTIFIAFDASELAATQAREWRNTLIVLSALAAVLLATLLAFFWYQRYQRSHRELLDAMKR
KEKLVAMGHLAAGVAHEIRNPLSSIKGLAKYFAERTPAGGESHELAQVMAKEADRLNRVVSELLELVKPAHLTLQAVNLN
DIITHSLNLVSQDAQSREIQLRFTANETLKRIQADPDRLTQVLLNLYLNAIHAIGRQGTITVEAKESGTDRVIITVTDSG
KGIAPDQLEAIFTPYFTTKADGTGLGLAVVQNIIEQHGGAIKVKSIEGKGAVFTIWLPVIARQQD
>Mature_464_residues
SFIRLHKDAAAMWLSRLLPAAIFILVGLFSIMVIRDYGRESAAARQTLLEKGNVLIRALESGTRVGMGMRMHHAQQQTLL
EEMAGQPGVLWFAVTDAQGVIITHSNPGMVGKSLYSPSEMHQLNPGPQECWRRVDVAANGETVPALEIYRQFQPLFGMRG
HGMRGHGMARSANDDEPAKQTIFIAFDASELAATQAREWRNTLIVLSALAAVLLATLLAFFWYQRYQRSHRELLDAMKRK
EKLVAMGHLAAGVAHEIRNPLSSIKGLAKYFAERTPAGGESHELAQVMAKEADRLNRVVSELLELVKPAHLTLQAVNLND
IITHSLNLVSQDAQSREIQLRFTANETLKRIQADPDRLTQVLLNLYLNAIHAIGRQGTITVEAKESGTDRVIITVTDSGK
GIAPDQLEAIFTPYFTTKADGTGLGLAVVQNIIEQHGGAIKVKSIEGKGAVFTIWLPVIARQQD

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain

Homologues:

Organism=Escherichia coli, GI1790436, Length=468, Percent_Identity=71.1538461538462, Blast_Score=654, Evalue=0.0,
Organism=Escherichia coli, GI1788549, Length=230, Percent_Identity=38.695652173913, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1790300, Length=225, Percent_Identity=30.2222222222222, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI48994928, Length=257, Percent_Identity=30.3501945525292, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1790346, Length=242, Percent_Identity=26.4462809917355, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786600, Length=257, Percent_Identity=26.0700389105058, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789808, Length=223, Percent_Identity=26.457399103139, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1786783, Length=222, Percent_Identity=26.5765765765766, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081816, Length=217, Percent_Identity=27.6497695852535, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI145693157, Length=229, Percent_Identity=29.6943231441048, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1786912, Length=218, Percent_Identity=26.605504587156, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87082128, Length=220, Percent_Identity=25.4545454545455, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788713, Length=253, Percent_Identity=25.6916996047431, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788393, Length=223, Percent_Identity=26.457399103139, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790861, Length=212, Percent_Identity=26.8867924528302, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ZRAS_SALTI (Q8Z332)

Other databases:

- EMBL:   AL627279
- EMBL:   AE014613
- RefSeq:   NP_457901.1
- RefSeq:   NP_807114.1
- ProteinModelPortal:   Q8Z332
- GeneID:   1067837
- GeneID:   1249968
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t3458
- KEGG:   sty:STY3712
- HOGENOM:   HBG467467
- OMA:   IITHSNP
- ProtClustDB:   PRK10364
- BioCyc:   SENT209261:T3458-MONOMER
- BioCyc:   SENT220341:STY3712-MONOMER
- BRENDA:   2.7.13.3
- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- Gene3D:   G3DSA:3.30.565.10
- PRINTS:   PR00344
- SMART:   SM00387
- SMART:   SM00388

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; SSF55874 ATP_bd_ATPase; SSF47384 His_kin_homodim

EC number: =2.7.13.3

Molecular weight: Translated: 51242; Mature: 51111

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x11b25fb8)-; HASH(0xfd70c78)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFIRLHKDAAAMWLSRLLPAAIFILVGLFSIMVIRDYGRESAAARQTLLEKGNVLIRAL
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEE
ESGTRVGMGMRMHHAQQQTLLEEMAGQPGVLWFAVTDAQGVIITHSNPGMVGKSLYSPSE
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCCCCHHHCCCHH
MHQLNPGPQECWRRVDVAANGETVPALEIYRQFQPLFGMRGHGMRGHGMARSANDDEPAK
HHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
QTIFIAFDASELAATQAREWRNTLIVLSALAAVLLATLLAFFWYQRYQRSHRELLDAMKR
EEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEKLVAMGHLAAGVAHEIRNPLSSIKGLAKYFAERTPAGGESHELAQVMAKEADRLNRVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
SELLELVKPAHLTLQAVNLNDIITHSLNLVSQDAQSREIQLRFTANETLKRIQADPDRLT
HHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHCCCHHHHH
QVLLNLYLNAIHAIGRQGTITVEAKESGTDRVIITVTDSGKGIAPDQLEAIFTPYFTTKA
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHCCCEECCC
DGTGLGLAVVQNIIEQHGGAIKVKSIEGKGAVFTIWLPVIARQQD
CCCCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEEHHHHCCCH
>Mature Secondary Structure 
SFIRLHKDAAAMWLSRLLPAAIFILVGLFSIMVIRDYGRESAAARQTLLEKGNVLIRAL
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEE
ESGTRVGMGMRMHHAQQQTLLEEMAGQPGVLWFAVTDAQGVIITHSNPGMVGKSLYSPSE
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCCCCHHHCCCHH
MHQLNPGPQECWRRVDVAANGETVPALEIYRQFQPLFGMRGHGMRGHGMARSANDDEPAK
HHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
QTIFIAFDASELAATQAREWRNTLIVLSALAAVLLATLLAFFWYQRYQRSHRELLDAMKR
EEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEKLVAMGHLAAGVAHEIRNPLSSIKGLAKYFAERTPAGGESHELAQVMAKEADRLNRVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
SELLELVKPAHLTLQAVNLNDIITHSLNLVSQDAQSREIQLRFTANETLKRIQADPDRLT
HHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHCCCHHHHH
QVLLNLYLNAIHAIGRQGTITVEAKESGTDRVIITVTDSGKGIAPDQLEAIFTPYFTTKA
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHCCCEECCC
DGTGLGLAVVQNIIEQHGGAIKVKSIEGKGAVFTIWLPVIARQQD
CCCCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEEHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504