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The map label for this gene is hydG

Identifier: 29143771

GI number: 29143771

Start: 3547029

End: 3548354

Strand: Reverse

Name: hydG

Synonym: t3457

Alternate gene names: 29143771

Gene position: 3548354-3547029 (Counterclockwise)

Preceding gene: 29143772

Following gene: 29143767

Centisome position: 74.05

GC content: 55.58

Gene sequence:

>1326_bases
GTGATACGCGGAAAAATCGATATTCTGGTTGTGGATGATGATGTAAGCCACTGCACGATTTTACAGGCGCTGCTTCGGGG
CTGGGGCTATAACGTCGCTCTGGCCTACAGTGGGCATGATGCGTTGGCTCAGGTGCGTGAGAAAGTCTTTGATCTGGTAC
TGTGCGATGTGCGTATGGCGGAGATGGACGGTATCGCCACGCTGAAGGAGATTAAAGCGCTCAACCCCGCCATTCCGATT
TTGATTATGACGGCATTCTCCAGCGTGGAAACGGCGGTAGAGGCGTTGAAAGCCGGCGCGCTCGACTATTTAATTAAACC
GCTGGATTTTGACCGTCTGCAGGAGACGCTGGAAAAGGCGTTGGCGCATACGCGGGAAACGGGCGCTGAATTGCCATCGG
CGTCGGCTGCGCAATTTGGCATGATTGGCAGCAGCCCGGCGATGCAACATTTATTGAATGAAATCGCGATGGTCGCACCA
TCAGACGCCACGGTATTGATTCACGGAGACTCCGGCACGGGTAAAGAGCTGGTGGCGCGGGCGCTGCACGCCTGTAGCGC
CAGGAGCGATAAACCGCTGGTCACGCTCAACTGCGCGGCGCTTAATGAATCGCTGCTGGAGTCTGAGCTGTTCGGACACG
AAAAAGGCGCGTTTACCGGCGCGGATAAACGTCGGGAGGGACGCTTTGTCGAAGCTGACGGCGGGACATTGTTTCTTGAT
GAAATCGGCGATATCTCGCCGTTAATGCAGGTACGTTTGCTGCGGGCGATTCAGGAGCGCGAAGTGCAACGCGTGGGAAG
TAACCAGACGATATCTGTGGATGTTTGGTTAATTGCCGCTACCCACCGCGACCTGGCGGAAGAGGTGAGTGCCGGTCGCT
TTCGCCAGGATCTCTATTACCGCCTGAATGTGGTGGCGATTGAAATGCCTTCTTTGCGCCAGCGTCGGGAAGATATTCCG
CTACTGGCAGACCATTTTCTGCGGCGTTTTGCCGAACGTAACCGAAAAGCGGTGAAGGGGTTTACGCCGCAGGCGATGGA
TCTGCTGATCCACTACGACTGGCCGGGTAATATCCGTGAGCTGGAAAACGCTATCGAACGAGCGGTGGTGTTGCTGACGG
GAGAATACATTTCTGAACGCGAATTGCCTCTCGCTATTGCCGCGACGCCGATAAAAGCGGAAAACAGCGCCGAAATCCAA
CCGTTGGTCGATGTGGAGAAAGAGGTGATTCTGGCGGCGCTGGAAAAAACGGGCGGCAACAAAACGGAAGCCGCCCGTCA
ATTGGGCATTACGCGCAAAACGCTGCTGGCTAAACTCAGCCGTTAG

Upstream 100 bases:

>100_bases
AACAGCATGGCGGCGCGATTAAGGTGAAAAGTATTGAGGGTAAAGGCGCGGTCTTTACTATCTGGTTGCCAGTGATAGCG
AGACAACAGGATTAACAAGG

Downstream 100 bases:

>100_bases
TTTTGCTCACGCTCAATGGCGCGCCAGCCAATGTCGTTACGGCTGAAGCTGCCGTCCCAGCGGATGTCGGTCATCAGGGC
GTAAGCGCGTTTCTGCGCTT

Product: transcriptional regulator ZraR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MIRGKIDILVVDDDVSHCTILQALLRGWGYNVALAYSGHDALAQVREKVFDLVLCDVRMAEMDGIATLKEIKALNPAIPI
LIMTAFSSVETAVEALKAGALDYLIKPLDFDRLQETLEKALAHTRETGAELPSASAAQFGMIGSSPAMQHLLNEIAMVAP
SDATVLIHGDSGTGKELVARALHACSARSDKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLD
EIGDISPLMQVRLLRAIQEREVQRVGSNQTISVDVWLIAATHRDLAEEVSAGRFRQDLYYRLNVVAIEMPSLRQRREDIP
LLADHFLRRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRELENAIERAVVLLTGEYISERELPLAIAATPIKAENSAEIQ
PLVDVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR

Sequences:

>Translated_441_residues
MIRGKIDILVVDDDVSHCTILQALLRGWGYNVALAYSGHDALAQVREKVFDLVLCDVRMAEMDGIATLKEIKALNPAIPI
LIMTAFSSVETAVEALKAGALDYLIKPLDFDRLQETLEKALAHTRETGAELPSASAAQFGMIGSSPAMQHLLNEIAMVAP
SDATVLIHGDSGTGKELVARALHACSARSDKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLD
EIGDISPLMQVRLLRAIQEREVQRVGSNQTISVDVWLIAATHRDLAEEVSAGRFRQDLYYRLNVVAIEMPSLRQRREDIP
LLADHFLRRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRELENAIERAVVLLTGEYISERELPLAIAATPIKAENSAEIQ
PLVDVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR
>Mature_441_residues
MIRGKIDILVVDDDVSHCTILQALLRGWGYNVALAYSGHDALAQVREKVFDLVLCDVRMAEMDGIATLKEIKALNPAIPI
LIMTAFSSVETAVEALKAGALDYLIKPLDFDRLQETLEKALAHTRETGAELPSASAAQFGMIGSSPAMQHLLNEIAMVAP
SDATVLIHGDSGTGKELVARALHACSARSDKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLD
EIGDISPLMQVRLLRAIQEREVQRVGSNQTISVDVWLIAATHRDLAEEVSAGRFRQDLYYRLNVVAIEMPSLRQRREDIP
LLADHFLRRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRELENAIERAVVLLTGEYISERELPLAIAATPIKAENSAEIQ
PLVDVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR

Specific function: Member of the two-component regulatory system zraS/zraR. When activated by zraS it acts in conjunction with sigma-54 to regulate the expression of zraP. Positively autoregulates the expression of the zraSR operon

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain

Homologues:

Organism=Escherichia coli, GI1790437, Length=441, Percent_Identity=88.8888888888889, Blast_Score=799, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=449, Percent_Identity=46.5478841870824, Blast_Score=384, Evalue=1e-107,
Organism=Escherichia coli, GI1788905, Length=438, Percent_Identity=44.2922374429224, Blast_Score=353, Evalue=1e-98,
Organism=Escherichia coli, GI1790299, Length=461, Percent_Identity=41.648590021692, Blast_Score=338, Evalue=3e-94,
Organism=Escherichia coli, GI87082117, Length=315, Percent_Identity=48.2539682539683, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI1789087, Length=315, Percent_Identity=47.9365079365079, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI87082152, Length=328, Percent_Identity=46.3414634146341, Blast_Score=254, Evalue=7e-69,
Organism=Escherichia coli, GI1789233, Length=335, Percent_Identity=40, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1786524, Length=319, Percent_Identity=43.2601880877743, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI87081872, Length=318, Percent_Identity=41.8238993710692, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI1787583, Length=337, Percent_Identity=36.2017804154303, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1789828, Length=249, Percent_Identity=37.7510040160643, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI87081858, Length=342, Percent_Identity=30.4093567251462, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789149, Length=112, Percent_Identity=33.9285714285714, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788191, Length=122, Percent_Identity=36.0655737704918, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ZRAR_SALTI (Q8Z333)

Other databases:

- EMBL:   AL627279
- EMBL:   AE014613
- RefSeq:   NP_457900.1
- RefSeq:   NP_807113.1
- ProteinModelPortal:   Q8Z333
- SMR:   Q8Z333
- GeneID:   1070829
- GeneID:   1249967
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t3457
- KEGG:   sty:STY3711
- HOGENOM:   HBG740884
- OMA:   GKSSAMQ
- ProtClustDB:   PRK10365
- BioCyc:   SENT209261:T3457-MONOMER
- BioCyc:   SENT220341:STY3711-MONOMER
- GO:   GO:0005737
- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789
- PRINTS:   PR01590
- SMART:   SM00382
- SMART:   SM00448

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat; SSF52172 CheY_like; SSF46689 Homeodomain_like

EC number: NA

Molecular weight: Translated: 48499; Mature: 48499

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50110 RESPONSE_REGULATORY; PS00675 SIGMA54_INTERACT_1; PS00676 SIGMA54_INTERACT_2; PS00688 SIGMA54_INTERACT_3; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRGKIDILVVDDDVSHCTILQALLRGWGYNVALAYSGHDALAQVREKVFDLVLCDVRMA
CCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
EMDGIATLKEIKALNPAIPILIMTAFSSVETAVEALKAGALDYLIKPLDFDRLQETLEKA
HHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHH
LAHTRETGAELPSASAAQFGMIGSSPAMQHLLNEIAMVAPSDATVLIHGDSGTGKELVAR
HHHHHHHHCCCCCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHH
ALHACSARSDKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLD
HHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEC
EIGDISPLMQVRLLRAIQEREVQRVGSNQTISVDVWLIAATHRDLAEEVSAGRFRQDLYY
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHE
RLNVVAIEMPSLRQRREDIPLLADHFLRRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRE
EEEEEEEECCHHHHHHCCCCHHHHHHHHHHHHCCHHHHCCCCCCCEEEEEEECCCCCHHH
LENAIERAVVLLTGEYISERELPLAIAATPIKAENSAEIQPLVDVEKEVILAALEKTGGN
HHHHHHHEEEEEECCHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
KTEAARQLGITRKTLLAKLSR
HHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MIRGKIDILVVDDDVSHCTILQALLRGWGYNVALAYSGHDALAQVREKVFDLVLCDVRMA
CCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
EMDGIATLKEIKALNPAIPILIMTAFSSVETAVEALKAGALDYLIKPLDFDRLQETLEKA
HHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHH
LAHTRETGAELPSASAAQFGMIGSSPAMQHLLNEIAMVAPSDATVLIHGDSGTGKELVAR
HHHHHHHHCCCCCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHH
ALHACSARSDKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLD
HHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEC
EIGDISPLMQVRLLRAIQEREVQRVGSNQTISVDVWLIAATHRDLAEEVSAGRFRQDLYY
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHE
RLNVVAIEMPSLRQRREDIPLLADHFLRRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRE
EEEEEEEECCHHHHHHCCCCHHHHHHHHHHHHCCHHHHCCCCCCCEEEEEEECCCCCHHH
LENAIERAVVLLTGEYISERELPLAIAATPIKAENSAEIQPLVDVEKEVILAALEKTGGN
HHHHHHHEEEEEECCHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
KTEAARQLGITRKTLLAKLSR
HHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504