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The map label for this gene is dapF [H]

Identifier: 29143676

GI number: 29143676

Start: 3452065

End: 3452889

Strand: Reverse

Name: dapF [H]

Synonym: t3350

Alternate gene names: 29143676

Gene position: 3452889-3452065 (Counterclockwise)

Preceding gene: 29143677

Following gene: 29143675

Centisome position: 72.06

GC content: 56.0

Gene sequence:

>825_bases
ATGCAATTCTCTAAAATGCATGGCCTTGGCAACGATTTTATGGTCGTCGACGCGGTAACGCAGAATGTCTTTTTTTCGCC
GGAACTGATTCGTCGGCTATCCGACAGACACTTGGGCGTAGGGTTCGATCAGCTGCTGGTGGTTGAGCCGCCCTATGATC
CTGAGTTGGACTTTCATTACCGCATCTTCAACGCCGACGGCAGTGAAGTCTCGCAGTGCGGCAATGGCGCGCGCTGTTTC
GCGCGATTTGTTCGCCTGAAAGGGCTAACCAATAAACGCGATATTCGGGTCAGTACCGCGAATGGCCGGATGGTACTGAG
CGTCACGGAAGATGAACTGGTGCGGGTGAACATGGGGGAGCCAAACTTTGAACCCGCGCAGGTGCCTTTTCGCGCCAACA
AAGCGGAAAAGACGTATATTATGCGAGCGGCGGAACAGACCATACTGTGCGGCGTGGTTTCAATGGGCAATCCGCACTGT
GTCATTCAGGTTGATAACGTCGACACGGCGGCCGTTGAAACACTGGGGCCGGTTCTGGAAAGCCATGAGCGTTTTCCGGA
ACGCGCCAACATTGGTTTTATGCAGGTTGTAAGACGTGAGCATATCCGGCTGCGAGTCTATGAACGCGGCGCAGGGGAGA
CCCGCGCGTGCGGCAGCGGCGCGTGCGCTGCCGTTGCCGTGGGGATTCAGCAGGGGCTGCTGGCTGAAGAAGTACGCGTG
GAATTACCGGGCGGTCGGCTGGATATCGCCTGGAAAGGTCCGGGTCATCCGTTATACATGACTGGTCCGGCGGCACATAT
CTACGACGGATTTATCCATTTATGA

Upstream 100 bases:

>100_bases
GTCCACGATGCCCGATAAGAACGATCGCGCAACGGGAGATGGCCCGTCCCAGGTCAATTACTGAGGATAGTTTTCTGTCC
CCGCGTGAACGGAGTGAATG

Downstream 100 bases:

>100_bases
AGCAACCAGAGGAAGAACTACAGGAAACGCTCACGGAACTGGACGATCGAGCGGTCGTCGATTACCTGCGCCACCACCCT
GAGTTTTTTATCCGTAACGC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLSDRHLGVGFDQLLVVEPPYDPELDFHYRIFNADGSEVSQCGNGARCF
ARFVRLKGLTNKRDIRVSTANGRMVLSVTEDELVRVNMGEPNFEPAQVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
VIQVDNVDTAAVETLGPVLESHERFPERANIGFMQVVRREHIRLRVYERGAGETRACGSGACAAVAVGIQQGLLAEEVRV
ELPGGRLDIAWKGPGHPLYMTGPAAHIYDGFIHL

Sequences:

>Translated_274_residues
MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLSDRHLGVGFDQLLVVEPPYDPELDFHYRIFNADGSEVSQCGNGARCF
ARFVRLKGLTNKRDIRVSTANGRMVLSVTEDELVRVNMGEPNFEPAQVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
VIQVDNVDTAAVETLGPVLESHERFPERANIGFMQVVRREHIRLRVYERGAGETRACGSGACAAVAVGIQQGLLAEEVRV
ELPGGRLDIAWKGPGHPLYMTGPAAHIYDGFIHL
>Mature_274_residues
MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLSDRHLGVGFDQLLVVEPPYDPELDFHYRIFNADGSEVSQCGNGARCF
ARFVRLKGLTNKRDIRVSTANGRMVLSVTEDELVRVNMGEPNFEPAQVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
VIQVDNVDTAAVETLGPVLESHERFPERANIGFMQVVRREHIRLRVYERGAGETRACGSGACAAVAVGIQQGLLAEEVRV
ELPGGRLDIAWKGPGHPLYMTGPAAHIYDGFIHL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=95.6204379562044, Blast_Score=550, Evalue=1e-158,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 30337; Mature: 30337

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLSDRHLGVGFDQLLVVEPPYDPELDFHY
CCCHHHCCCCCCEEEEEEECCCEEECHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEE
RIFNADGSEVSQCGNGARCFARFVRLKGLTNKRDIRVSTANGRMVLSVTEDELVRVNMGE
EEEECCCHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCCEEEEECCC
PNFEPAQVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHCVIQVDNVDTAAVETLGPVLE
CCCCCCCCCEECCCCCHHHHHHHHCCEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHH
SHERFPERANIGFMQVVRREHIRLRVYERGAGETRACGSGACAAVAVGIQQGLLAEEVRV
HHHCCCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEHEEE
ELPGGRLDIAWKGPGHPLYMTGPAAHIYDGFIHL
ECCCCEEEEEEECCCCEEEEECCHHHHHCCEECC
>Mature Secondary Structure
MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLSDRHLGVGFDQLLVVEPPYDPELDFHY
CCCHHHCCCCCCEEEEEEECCCEEECHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEE
RIFNADGSEVSQCGNGARCFARFVRLKGLTNKRDIRVSTANGRMVLSVTEDELVRVNMGE
EEEECCCHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEECCCCEEEEECCC
PNFEPAQVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHCVIQVDNVDTAAVETLGPVLE
CCCCCCCCCEECCCCCHHHHHHHHCCEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHH
SHERFPERANIGFMQVVRREHIRLRVYERGAGETRACGSGACAAVAVGIQQGLLAEEVRV
HHHCCCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEHEEE
ELPGGRLDIAWKGPGHPLYMTGPAAHIYDGFIHL
ECCCCEEEEEEECCCCEEEEECCHHHHHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA