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The map label for this gene is yhdG [H]

Identifier: 29143632

GI number: 29143632

Start: 3394487

End: 3395332

Strand: Direct

Name: yhdG [H]

Synonym: t3299

Alternate gene names: 29143632

Gene position: 3394487-3395332 (Clockwise)

Preceding gene: 29143631

Following gene: 29143633

Centisome position: 70.84

GC content: 53.66

Gene sequence:

>846_bases
ATGATGTCTTCTAACCCGCAAGTGTGGGAAAGCGATAAATCTCGTTTACGGATGGTGCACGTTGATGAGCCAGGAATTCG
CACGGTGCAAATTGCCGGTAGCGACCCTGTTGAGATGGCCGATGCCGCACGTATTAACGTGGAAAGCGGCGCCCAGATTA
TTGATATCAATATGGGGTGCCCGGCGAAGAAAGTGAATCGTAAGCTGGCAGGTTCAGCCCTCTTGCAGTACCCGGATTTA
GTGAAGTCTATACTCATCGGGGTCGTAAACGCGGTGGACGTTCCTGTGACTCTCAAGATTCGCACCGGCTGGGCGCCGGA
ACACCGTAACTGCGTAGAGATTGCCCAACTGGCTGAAGATTGTGGTATTCAGGCCCTGACCATCCATGGCCGCACCCGCG
CCTGTTTGTTCAACGGAGAAGCTGAATACGACAGTATTCGGGCAGTTAAGCAGAAAGTTTCCATTCCGATTATCGCGAAT
GGCGACATTACTAACCCGCATAAAGCCAGAGCTGTTCTCGACTATACAGGGGCGGATGCCCTGATGATAGGCCGCGCAGC
TCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTATCTGGACACTGGGGAGCTGCTTCCGCCGCTGCCGCTGGCAG
AGGTAAAGCGCTTGCTTTGCACGCATGTTCGGGAACTGCATGACTTTTACGGTCAGGCAAAAGGGTACCGAATCGCGCGT
AAACACGTCTCCTGGTATCTCCAGGAACACGCTCCAGACGACCAGTTTCGGCGCACATTCAACGCTATTGAGGACGCCAG
CGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTTGCGTAA

Upstream 100 bases:

>100_bases
GCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACTGACAGACCTTTCCGGACGCTGTGCTATGAGATGGGAG
CAGGATTGACAGTATCCGAG

Downstream 100 bases:

>100_bases
ACAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTC
AGGATCAGGTAACCCAAAAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MMSSNPQVWESDKSRLRMVHVDEPGIRTVQIAGSDPVEMADAARINVESGAQIIDINMGCPAKKVNRKLAGSALLQYPDL
VKSILIGVVNAVDVPVTLKIRTGWAPEHRNCVEIAQLAEDCGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPIIAN
GDITNPHKARAVLDYTGADALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCTHVRELHDFYGQAKGYRIAR
KHVSWYLQEHAPDDQFRRTFNAIEDASEQLEALEAYFENFA

Sequences:

>Translated_281_residues
MMSSNPQVWESDKSRLRMVHVDEPGIRTVQIAGSDPVEMADAARINVESGAQIIDINMGCPAKKVNRKLAGSALLQYPDL
VKSILIGVVNAVDVPVTLKIRTGWAPEHRNCVEIAQLAEDCGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPIIAN
GDITNPHKARAVLDYTGADALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCTHVRELHDFYGQAKGYRIAR
KHVSWYLQEHAPDDQFRRTFNAIEDASEQLEALEAYFENFA
>Mature_281_residues
MMSSNPQVWESDKSRLRMVHVDEPGIRTVQIAGSDPVEMADAARINVESGAQIIDINMGCPAKKVNRKLAGSALLQYPDL
VKSILIGVVNAVDVPVTLKIRTGWAPEHRNCVEIAQLAEDCGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPIIAN
GDITNPHKARAVLDYTGADALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCTHVRELHDFYGQAKGYRIAR
KHVSWYLQEHAPDDQFRRTFNAIEDASEQLEALEAYFENFA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=261, Percent_Identity=31.4176245210728, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI239788483, Length=180, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI239788462, Length=182, Percent_Identity=34.0659340659341, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI40807366, Length=175, Percent_Identity=33.7142857142857, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI8923374, Length=178, Percent_Identity=33.7078651685393, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1789660, Length=281, Percent_Identity=95.729537366548, Blast_Score=560, Evalue=1e-161,
Organism=Escherichia coli, GI1788462, Length=254, Percent_Identity=31.1023622047244, Blast_Score=107, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI25144369, Length=199, Percent_Identity=35.1758793969849, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17507177, Length=195, Percent_Identity=30.7692307692308, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17543114, Length=182, Percent_Identity=33.5164835164835, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17510279, Length=178, Percent_Identity=34.8314606741573, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=198, Percent_Identity=28.2828282828283, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323433, Length=220, Percent_Identity=30.9090909090909, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323437, Length=146, Percent_Identity=32.8767123287671, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45549423, Length=145, Percent_Identity=40.6896551724138, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24580595, Length=187, Percent_Identity=32.620320855615, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19920448, Length=187, Percent_Identity=32.620320855615, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585320, Length=176, Percent_Identity=32.3863636363636, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19921524, Length=177, Percent_Identity=29.3785310734463, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 31363; Mature: 31363

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSSNPQVWESDKSRLRMVHVDEPGIRTVQIAGSDPVEMADAARINVESGAQIIDINMGC
CCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHEEECCCCCEEEEECCCC
PAKKVNRKLAGSALLQYPDLVKSILIGVVNAVDVPVTLKIRTGWAPEHRNCVEIAQLAED
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHH
CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPIIANGDITNPHKARAVLDYTGADA
CCCEEEEECCCEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCHHHEEEEEECCCCE
LMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCTHVRELHDFYGQAKGYRIAR
EEECCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
KHVSWYLQEHAPDDQFRRTFNAIEDASEQLEALEAYFENFA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMSSNPQVWESDKSRLRMVHVDEPGIRTVQIAGSDPVEMADAARINVESGAQIIDINMGC
CCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHEEECCCCCEEEEECCCC
PAKKVNRKLAGSALLQYPDLVKSILIGVVNAVDVPVTLKIRTGWAPEHRNCVEIAQLAED
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHH
CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPIIANGDITNPHKARAVLDYTGADA
CCCEEEEECCCEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCCCHHHEEEEEECCCCE
LMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCTHVRELHDFYGQAKGYRIAR
EEECCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
KHVSWYLQEHAPDDQFRRTFNAIEDASEQLEALEAYFENFA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]