Click here to switch to the map view.

The map label for this gene is rpoN [H]

Identifier: 29143571

GI number: 29143571

Start: 3324431

End: 3325864

Strand: Direct

Name: rpoN [H]

Synonym: t3237

Alternate gene names: 29143571

Gene position: 3324431-3325864 (Clockwise)

Preceding gene: 29143570

Following gene: 29143572

Centisome position: 69.38

GC content: 54.04

Gene sequence:

>1434_bases
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCTCAGCTACAACAGGCCATCCGTCTGTTGCA
GTTGTCTACGCTGGAACTTCAGCAGGAACTCCAGCAAGCGCTGGAAAATAACCCGCTGCTTGAGCAAACCGATCTTCATG
ACGAAATCGACACTCAGCAACCTCAGGATGACGATCCTCTCGATACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTTGACGCCAGTTGGGATGAAATTTACACCGCCGGGACGCCGTCCGGCCCCAGCGGCGATTATATCGACGA
TGAGCTGCCCGTCTATCAGGGCGAAACGACGCAGTCGTTGCAGGATTATCTGATGTGGCAGGTTGAGCTAACGCCCTTCT
CGGACACCGATCGCGCTATTGCGACATCCATTGTCGACGCGGTAGATGATACCGGCTATCTCACCGTATCCCTGGACGAA
ATTCGCGAAAGCATGGGCGATGTAGAGGTGGATCTCGATGAGGTCGAAGCCGTCCTGAAGCGTATTCAGCGTTTTGATCC
GGTAGGCGTCGCGGCAAAAGATCTTCGCGACTGTCTGCTGATCCAGCTTTCACAATTCGACAAATCCACGCCGTGGCTGG
AAGAGGCGCGGCTTATTATCTGCGATCACCTTGATCTGCTGGCCAACCACGATTTCCGCACGTTGATGCGCGTTACCCGA
CTAAAAGAAGAGGTGCTGAAAGAGGCGGTAAACCTGATTCAGTCGCTCGATCCCAGACCCGGTCAATCGATCCAGACCGG
CGAGCCGGAGTACGTTATCCCGGATGTGCTGGTACGCAAGCATAACGGTCGCTGGACGGTGGAGCTTAATAGCGACAGTA
TTCCCCGTTTACAGATTAACCAGCACTATGCCGCCATGTGCAATAGCGCGCGCAACGATGCCGACAGCCAGTTCATCCGC
AGTAATTTACAGGATGCGAAATGGCTGATAAAAAGCCTTGAAAGCCGTAACGACACGCTGCTGCGCGTCAGTCGCTGCAT
CGTCGAGCAACAGCAAGCCTTTTTTGAACAGGGCGAAGAATATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAGATGCACGAATCCACTATATCCCGCGTGACCACGCAGAAGTATTTGCACAGCCCGCGCGGTATTTTTGAACTCAAA
TATTTCTTTTCCAGCCACGTCAATACCGAAGGCGGAGGCGAAGCCTCTTCCACCGCGATTCGCGCGCTGGTGAAGAAGTT
AATTGCGGCGGAAAACCCCGCGAAACCACTGAGCGACAGCAAGTTAACCTCTCTGCTGTCAGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAACGCAAACAGCTGGTTTGA

Upstream 100 bases:

>100_bases
TGCAAGACGAACACGTTAAGCGTGTATATCTTGGGGAAGACTTCAGACTCTGATAGGGTAGAGGGCTATGCTGCTCTAGC
GGGAGAAAACGACTCTGAAT

Downstream 100 bases:

>100_bases
CCCAACCGATAAGGAAGACACTATGCAGCTCAATATCACCGGACATAACGTCGAAATTACTGAAGCCCTGCGTGAATTTG
TGACGACGAAATTCGCCAAA

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALENNPLLEQTDLHDEIDTQQPQDDDPLDTADALEQKEMPE
ELPLDASWDEIYTAGTPSGPSGDYIDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVSLDE
IRESMGDVEVDLDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKSTPWLEEARLIICDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGRWTVELNSDSIPRLQINQHYAAMCNSARNDADSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Sequences:

>Translated_477_residues
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALENNPLLEQTDLHDEIDTQQPQDDDPLDTADALEQKEMPE
ELPLDASWDEIYTAGTPSGPSGDYIDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVSLDE
IRESMGDVEVDLDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKSTPWLEEARLIICDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGRWTVELNSDSIPRLQINQHYAAMCNSARNDADSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV
>Mature_477_residues
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALENNPLLEQTDLHDEIDTQQPQDDDPLDTADALEQKEMPE
ELPLDASWDEIYTAGTPSGPSGDYIDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVSLDE
IRESMGDVEVDLDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKSTPWLEEARLIICDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGRWTVELNSDSIPRLQINQHYAAMCNSARNDADSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of enzymes involved in arginine catabolism. The open complex (sigma-

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=477, Percent_Identity=93.9203354297694, Blast_Score=920, Evalue=0.0,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 53989; Mature: 53989

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALENNPLLEQTDLHDEIDTQQ
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCC
PQDDDPLDTADALEQKEMPEELPLDASWDEIYTAGTPSGPSGDYIDDELPVYQGETTQSL
CCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHH
QDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVSLDEIRESMGDVEVDLDEVEAVLK
HHHHEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCEECHHHHHHHHH
RIQRFDPVGVAAKDLRDCLLIQLSQFDKSTPWLEEARLIICDHLDLLANHDFRTLMRVTR
HHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCHHHHHHHHHHHHCCCHHHHHHHHH
LKEEVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGRWTVELNSDSIPRLQIN
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCHHHHEECCCEEEEEECCCCCCEEEEC
QHYAAMCNSARNDADSQFIRSNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
YMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELKYFFSSHVNTEGGGEASSTAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHH
RALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV
HHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALENNPLLEQTDLHDEIDTQQ
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCC
PQDDDPLDTADALEQKEMPEELPLDASWDEIYTAGTPSGPSGDYIDDELPVYQGETTQSL
CCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHH
QDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVSLDEIRESMGDVEVDLDEVEAVLK
HHHHEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCEECHHHHHHHHH
RIQRFDPVGVAAKDLRDCLLIQLSQFDKSTPWLEEARLIICDHLDLLANHDFRTLMRVTR
HHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCHHHHHHHHHHHHCCCHHHHHHHHH
LKEEVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGRWTVELNSDSIPRLQIN
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCHHHHEECCCEEEEEECCCCCCEEEEC
QHYAAMCNSARNDADSQFIRSNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
YMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELKYFFSSHVNTEGGGEASSTAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHH
RALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV
HHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203540; 8444818; 8025669; 7876255; 9278503 [H]