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The map label for this gene is mlaD [H]

Identifier: 29143562

GI number: 29143562

Start: 3317621

End: 3318172

Strand: Reverse

Name: mlaD [H]

Synonym: t3228

Alternate gene names: 29143562

Gene position: 3318172-3317621 (Counterclockwise)

Preceding gene: 29143563

Following gene: 29143561

Centisome position: 69.24

GC content: 51.45

Gene sequence:

>552_bases
ATGCAAACGAAAAAAAATGAAATTTGGGTAGGCGTTTTTTTGCTGGTAGCGTTGCTGGCGGCGCTGTTTGTTTGCCTGAA
GGCGGCAAATGTGACCTCTATGCGCACGGAGCCGACCTATAAGGTTTATGCGACCTTCGATAACATCGGCGGTCTGAAAG
TGCGTTCCCCGGTACGCATCGGCGGGGTGGTTGTCGGGCGGGTTGAAGATATCTCGCTTGATCCGAAAACATACCTGCCG
CGCGTAACGCTCGACATCGAAGAGCGTTATAACCATATTCCCGATACCAGCTCGTTGAGTATTCGTACTTCCGGGCTGCT
GGGGGAGCAATATCTGGCATTAAACGTCGGTTTTGAAGATCCTGAGCTGGGAACGTCTATCCTCAAAGATGGGAGTACGA
TTCAGGACACCAAGTCTGCAATGGTGCTGGAAGATATGATTGGCCAGTTCCTTTATAACAGCAAAGGCGACGACAATAAG
AATTCTGGCGATGCGCCAGCCGCAACGGAAGGCCATACTGAAGCCACAACGCCCGCGGGCGAAACGAAATAA

Upstream 100 bases:

>100_bases
GGGATTAGCCGGGCAACGACGCGTACCGTGGTTCACGCATCGTTGGCCGTTCTGGGGCTGGATTTTGTGCTGACCGCACT
GATGTTTGGGAATTGAGTTC

Downstream 100 bases:

>100_bases
TCTCAGGAGAATTGAGGCATGTTTAAACGACTGATGATGGTGGCCCTGCTGGTCATTGCGCCATTAAGCGCAGCAACGGC
AGCGGATCAGAGCAATCCTT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MQTKKNEIWVGVFLLVALLAALFVCLKAANVTSMRTEPTYKVYATFDNIGGLKVRSPVRIGGVVVGRVEDISLDPKTYLP
RVTLDIEERYNHIPDTSSLSIRTSGLLGEQYLALNVGFEDPELGTSILKDGSTIQDTKSAMVLEDMIGQFLYNSKGDDNK
NSGDAPAATEGHTEATTPAGETK

Sequences:

>Translated_183_residues
MQTKKNEIWVGVFLLVALLAALFVCLKAANVTSMRTEPTYKVYATFDNIGGLKVRSPVRIGGVVVGRVEDISLDPKTYLP
RVTLDIEERYNHIPDTSSLSIRTSGLLGEQYLALNVGFEDPELGTSILKDGSTIQDTKSAMVLEDMIGQFLYNSKGDDNK
NSGDAPAATEGHTEATTPAGETK
>Mature_183_residues
MQTKKNEIWVGVFLLVALLAALFVCLKAANVTSMRTEPTYKVYATFDNIGGLKVRSPVRIGGVVVGRVEDISLDPKTYLP
RVTLDIEERYNHIPDTSSLSIRTSGLLGEQYLALNVGFEDPELGTSILKDGSTIQDTKSAMVLEDMIGQFLYNSKGDDNK
NSGDAPAATEGHTEATTPAGETK

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG1463

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, periplasmic component

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein; Periplasmic side (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaD family [H]

Homologues:

Organism=Escherichia coli, GI1789584, Length=183, Percent_Identity=87.431693989071, Blast_Score=316, Evalue=6e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003399 [H]

Pfam domain/function: PF02470 MCE [H]

EC number: NA

Molecular weight: Translated: 19827; Mature: 19827

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTKKNEIWVGVFLLVALLAALFVCLKAANVTSMRTEPTYKVYATFDNIGGLKVRSPVRI
CCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCEEECCCEEE
GGVVVGRVEDISLDPKTYLPRVTLDIEERYNHIPDTSSLSIRTSGLLGEQYLALNVGFED
CCEEEEEEECCCCCCHHHCCEEEEEHHHHHCCCCCCCCEEEEECCCCCCEEEEEECCCCC
PELGTSILKDGSTIQDTKSAMVLEDMIGQFLYNSKGDDNKNSGDAPAATEGHTEATTPAG
CHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ETK
CCC
>Mature Secondary Structure
MQTKKNEIWVGVFLLVALLAALFVCLKAANVTSMRTEPTYKVYATFDNIGGLKVRSPVRI
CCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCEEECCCEEE
GGVVVGRVEDISLDPKTYLPRVTLDIEERYNHIPDTSSLSIRTSGLLGEQYLALNVGFED
CCEEEEEEECCCCCCHHHCCEEEEEHHHHHCCCCCCCCEEEEECCCCCCEEEEEECCCCC
PELGTSILKDGSTIQDTKSAMVLEDMIGQFLYNSKGDDNKNSGDAPAATEGHTEATTPAG
CHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ETK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]