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The map label for this gene is dnaG [H]

Identifier: 29143471

GI number: 29143471

Start: 3217501

End: 3219246

Strand: Direct

Name: dnaG [H]

Synonym: t3130

Alternate gene names: 29143471

Gene position: 3217501-3219246 (Clockwise)

Preceding gene: 29143470

Following gene: 29143472

Centisome position: 67.14

GC content: 51.72

Gene sequence:

>1746_bases
ATGGCTGGACGAATCCCACGCGTATTTATTAATGACCTGCTGGCGAGAACCGATATCGTCGATCTGATCGACGTGCGCGT
AAAACTCAAGAAACAGGGCAAGAACTATCACGCGTGTTGTCCATTCCATAATGAGAAAACCCCTTCTTTCACCGTAAACG
GTGAAAAACAGTTTTATCACTGCTTTGGATGCGGCGCGCATGGCAATGCGATCGACTTTTTGATGAATTACGACAAGCTC
GAATTTGTCGAAACCGTCGAAGAATTAGCGGCCATGCATAACCTGGAAATTCCCTATGAAGCAGGCACCGGGCTTAGCCA
GATAGAGCGCCATCAACGGCAAAACCTCTATCAGTTGATGAATGGTCTGAATGACTTTTATCAGCAGTCGCTTACCCATC
CTGCGGCAAAACCTGCGCGTGACTATCTGCAAAAGCGCGGTCTGAGCGCTGAAATTATTCAGCGTTTCGCCATTGGCTTC
GCGCCGCCTGGTTGGGATAACGCGTTAAAGCGCTTCGGTAATAACAGCGATAACAAGGCATTGTTGCTGGATGCCGGAAT
GCTGGTCAATAACGAGCAGGGCAGCACTTACGATCGTTTCCGTAATCGGGTCATGTTCCCTATTCGGGACAAACGCGGCA
GGGTGATTGGTTTTGGCGGTCGAGTTCTGGGGAACGACACGCCGAAATACCTTAACTCTCCGGAAACGGACATTTTCCAT
AAAGGCCGCCAGCTATATGGTCTTTATGAGGCTCAGCAATATAGTGCTGAACCGCAACGCTTGCTTGTGGTCGAAGGTTA
TATGGATGTCGTCGCACTAGCGCAATACGACATTAACTATGCGGTCGCCTCGCTGGGTACCTCAACCACGGCAGACCATA
TGCATATGTTATTCCGGGCGACGAACAACGTAATTTGCTGTTACGACGGCGACCGGGCTGGCCGTGATGCCGCCTGGAGA
GCGCTGGAAACAGCAATGCCTTATATGACCGACGGTCGGCAGGTACGCTTTATGTTTTTACCTGACGGCGAAGACCCGGA
TACGCTGGTGCGTAAAGAAGGCAAGGCGGCTTTTGAAGCGCGGATGGAGCAGGCGCAGCCGCTCTCTACGTTTCTGTTTA
ATAGCCTCCTGCCGCAGGTGGATTTAAGCTCGCCGGATGGCAGCACGCAGTTAGCCGCGCTGGCGCTGCCGCTCATTAAT
CAGGTGCCTGGCGACACGCACCGTATTCAGCTGCGCCAGACGTTGGGGCTGAAGTTAGGCATTTTCGATGATAGTCAGCT
TGATCGTTTAGTGCCAAAACAGGCGGAAAGCGGCGTTTCCCGCCCGGCTCCGCAGCTAAAACGCACGACCATGCGTATAC
TGATAGGATTACTGGTGCAAAATCCGGATCTCGCGCCGTTAGTGCCGCCCTTAGACGCGCTGGATCAGAATAAGCTGCCT
GGACTTGGCTTATTTAAAGAATTGGTCAAAACTTGTTTGGCTCAGCCAGGTCTTACTACCGGACAACTTCTGGAACTCTA
CCGTGGCACAAATGATGCTGCGACCCTTGAAAAATTATCGATGTGGGACGATATAGCAGATAAGGCGATTGCCGAAAAGA
CCTTTACCGACTCACTCAACCATATGTTTGATTCGCTGCTGCAACTGCGACAAGAAGAGCTGATCGCTCGCGATCGCACT
CACGGTTTAAGCAGCGAAGAACGCCGAGAGCTCTGGACGTTAAACCAGGAGCTGGCCAGAAAATGA

Upstream 100 bases:

>100_bases
AGAGAGCAAGTCCCCGGCGCTTACGCAAGTCAGCGACAGGGGTGAGCAAACGTAGCCAACACTACGGCAGCCTGAAGAAT
GACGTGTATAAAAGGGGCAT

Downstream 100 bases:

>100_bases
ATTTAACGGCTTAAATGCCGCATATCGTTCGGGAAGCCCCCGACAGCCGCAGTGAGAGGCAGCGGCCAAAATATAAGTAT
GCCCTCGCTTTAAATGTCGA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 581; Mature: 580

Protein sequence:

>581_residues
MAGRIPRVFINDLLARTDIVDLIDVRVKLKKQGKNYHACCPFHNEKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKL
EFVETVEELAAMHNLEIPYEAGTGLSQIERHQRQNLYQLMNGLNDFYQQSLTHPAAKPARDYLQKRGLSAEIIQRFAIGF
APPGWDNALKRFGNNSDNKALLLDAGMLVNNEQGSTYDRFRNRVMFPIRDKRGRVIGFGGRVLGNDTPKYLNSPETDIFH
KGRQLYGLYEAQQYSAEPQRLLVVEGYMDVVALAQYDINYAVASLGTSTTADHMHMLFRATNNVICCYDGDRAGRDAAWR
ALETAMPYMTDGRQVRFMFLPDGEDPDTLVRKEGKAAFEARMEQAQPLSTFLFNSLLPQVDLSSPDGSTQLAALALPLIN
QVPGDTHRIQLRQTLGLKLGIFDDSQLDRLVPKQAESGVSRPAPQLKRTTMRILIGLLVQNPDLAPLVPPLDALDQNKLP
GLGLFKELVKTCLAQPGLTTGQLLELYRGTNDAATLEKLSMWDDIADKAIAEKTFTDSLNHMFDSLLQLRQEELIARDRT
HGLSSEERRELWTLNQELARK

Sequences:

>Translated_581_residues
MAGRIPRVFINDLLARTDIVDLIDVRVKLKKQGKNYHACCPFHNEKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKL
EFVETVEELAAMHNLEIPYEAGTGLSQIERHQRQNLYQLMNGLNDFYQQSLTHPAAKPARDYLQKRGLSAEIIQRFAIGF
APPGWDNALKRFGNNSDNKALLLDAGMLVNNEQGSTYDRFRNRVMFPIRDKRGRVIGFGGRVLGNDTPKYLNSPETDIFH
KGRQLYGLYEAQQYSAEPQRLLVVEGYMDVVALAQYDINYAVASLGTSTTADHMHMLFRATNNVICCYDGDRAGRDAAWR
ALETAMPYMTDGRQVRFMFLPDGEDPDTLVRKEGKAAFEARMEQAQPLSTFLFNSLLPQVDLSSPDGSTQLAALALPLIN
QVPGDTHRIQLRQTLGLKLGIFDDSQLDRLVPKQAESGVSRPAPQLKRTTMRILIGLLVQNPDLAPLVPPLDALDQNKLP
GLGLFKELVKTCLAQPGLTTGQLLELYRGTNDAATLEKLSMWDDIADKAIAEKTFTDSLNHMFDSLLQLRQEELIARDRT
HGLSSEERRELWTLNQELARK
>Mature_580_residues
AGRIPRVFINDLLARTDIVDLIDVRVKLKKQGKNYHACCPFHNEKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLE
FVETVEELAAMHNLEIPYEAGTGLSQIERHQRQNLYQLMNGLNDFYQQSLTHPAAKPARDYLQKRGLSAEIIQRFAIGFA
PPGWDNALKRFGNNSDNKALLLDAGMLVNNEQGSTYDRFRNRVMFPIRDKRGRVIGFGGRVLGNDTPKYLNSPETDIFHK
GRQLYGLYEAQQYSAEPQRLLVVEGYMDVVALAQYDINYAVASLGTSTTADHMHMLFRATNNVICCYDGDRAGRDAAWRA
LETAMPYMTDGRQVRFMFLPDGEDPDTLVRKEGKAAFEARMEQAQPLSTFLFNSLLPQVDLSSPDGSTQLAALALPLINQ
VPGDTHRIQLRQTLGLKLGIFDDSQLDRLVPKQAESGVSRPAPQLKRTTMRILIGLLVQNPDLAPLVPPLDALDQNKLPG
LGLFKELVKTCLAQPGLTTGQLLELYRGTNDAATLEKLSMWDDIADKAIAEKTFTDSLNHMFDSLLQLRQEELIARDRTH
GLSSEERRELWTLNQELARK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=581, Percent_Identity=86.4027538726334, Blast_Score=1055, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 65393; Mature: 65262

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRIPRVFINDLLARTDIVDLIDVRVKLKKQGKNYHACCPFHNEKTPSFTVNGEKQFYH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEECCCCEEEE
CFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEIPYEAGTGLSQIERHQRQNLYQLM
EEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
NGLNDFYQQSLTHPAAKPARDYLQKRGLSAEIIQRFAIGFAPPGWDNALKRFGNNSDNKA
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCE
LLLDAGMLVNNEQGSTYDRFRNRVMFPIRDKRGRVIGFGGRVLGNDTPKYLNSPETDIFH
EEEECCEEEECCCCCHHHHHHCCEECEEECCCCCEEEECCEECCCCCHHHCCCCCHHHHH
KGRQLYGLYEAQQYSAEPQRLLVVEGYMDVVALAQYDINYAVASLGTSTTADHMHMLFRA
CCCHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
TNNVICCYDGDRAGRDAAWRALETAMPYMTDGRQVRFMFLPDGEDPDTLVRKEGKAAFEA
CCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHCCHHHHHH
RMEQAQPLSTFLFNSLLPQVDLSSPDGSTQLAALALPLINQVPGDTHRIQLRQTLGLKLG
HHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHCCCEEE
IFDDSQLDRLVPKQAESGVSRPAPQLKRTTMRILIGLLVQNPDLAPLVPPLDALDQNKLP
CCCCHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
GLGLFKELVKTCLAQPGLTTGQLLELYRGTNDAATLEKLSMWDDIADKAIAEKTFTDSLN
CHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HMFDSLLQLRQEELIARDRTHGLSSEERRELWTLNQELARK
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AGRIPRVFINDLLARTDIVDLIDVRVKLKKQGKNYHACCPFHNEKTPSFTVNGEKQFYH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEECCCCEEEE
CFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEIPYEAGTGLSQIERHQRQNLYQLM
EEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
NGLNDFYQQSLTHPAAKPARDYLQKRGLSAEIIQRFAIGFAPPGWDNALKRFGNNSDNKA
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCE
LLLDAGMLVNNEQGSTYDRFRNRVMFPIRDKRGRVIGFGGRVLGNDTPKYLNSPETDIFH
EEEECCEEEECCCCCHHHHHHCCEECEEECCCCCEEEECCEECCCCCHHHCCCCCHHHHH
KGRQLYGLYEAQQYSAEPQRLLVVEGYMDVVALAQYDINYAVASLGTSTTADHMHMLFRA
CCCHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
TNNVICCYDGDRAGRDAAWRALETAMPYMTDGRQVRFMFLPDGEDPDTLVRKEGKAAFEA
CCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHCCHHHHHH
RMEQAQPLSTFLFNSLLPQVDLSSPDGSTQLAALALPLINQVPGDTHRIQLRQTLGLKLG
HHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHCCCEEE
IFDDSQLDRLVPKQAESGVSRPAPQLKRTTMRILIGLLVQNPDLAPLVPPLDALDQNKLP
CCCCHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
GLGLFKELVKTCLAQPGLTTGQLLELYRGTNDAATLEKLSMWDDIADKAIAEKTFTDSLN
CHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HMFDSLLQLRQEELIARDRTHGLSSEERRELWTLNQELARK
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3005129; 11677609 [H]