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The map label for this gene is hypA

Identifier: 29143409

GI number: 29143409

Start: 3148864

End: 3149205

Strand: Reverse

Name: hypA

Synonym: t3065

Alternate gene names: 29143409

Gene position: 3149205-3148864 (Counterclockwise)

Preceding gene: 29143410

Following gene: 29143408

Centisome position: 65.72

GC content: 56.14

Gene sequence:

>342_bases
ATGCATGAGCTGTCTCTTTGCCAAAGCGCCGTTGAAATTATTCAGCAGCAGGCAGAACAGCACGGCGTGGCGCGCGTCAC
CGGCGTATGGCTGGAGATAGGCGCGCTTTCGTGCGTTGAAGAGCGCGCGGTTCGTTTCAGCTTTGACATCGCCTGCCAGG
GGACTTTAGCGCAAGGGTGCGAATTGCACATCGACTACAGACCGGCCCAGGCCTGGTGCTGGGATTGCAGTCAGGTGGTG
GAAATTCTCAGGCATGACGCGCAATGTCCGCATTGTCATGGCGATCGCCTGCGGGTGGATACCGGCGATTCGTTGAAAGT
AAAAAGTATTGAGGTCGAATAA

Upstream 100 bases:

>100_bases
GATGACTGCGCGCGGATGCTGTTATCCCTGCCGGTAAGCAATCCTGATGCGCCGCAGACAAGCCGGCGTGCGTTGCTGTT
TGGCCGTCGGAGCTGTGAGA

Downstream 100 bases:

>100_bases
CCCGCATTTTATTTCAGGTGGTTAATTATGTGTATTGGCGTCCCCGGTCAGGTGCTGGCCGTCGGAGATGATATTCACCA
GCTTGCGCAGGTGGAAGTGT

Product: hydrogenase nickel incorporation protein HybF

Products: NA

Alternate protein names: Hydrogenase-2 operon protein hybF

Number of amino acids: Translated: 113; Mature: 113

Protein sequence:

>113_residues
MHELSLCQSAVEIIQQQAEQHGVARVTGVWLEIGALSCVEERAVRFSFDIACQGTLAQGCELHIDYRPAQAWCWDCSQVV
EILRHDAQCPHCHGDRLRVDTGDSLKVKSIEVE

Sequences:

>Translated_113_residues
MHELSLCQSAVEIIQQQAEQHGVARVTGVWLEIGALSCVEERAVRFSFDIACQGTLAQGCELHIDYRPAQAWCWDCSQVV
EILRHDAQCPHCHGDRLRVDTGDSLKVKSIEVE
>Mature_113_residues
MHELSLCQSAVEIIQQQAEQHGVARVTGVWLEIGALSCVEERAVRFSFDIACQGTLAQGCELHIDYRPAQAWCWDCSQVV
EILRHDAQCPHCHGDRLRVDTGDSLKVKSIEVE

Specific function: Probably plays a role in an hydrogenase nickel cofactor insertion step

COG id: COG0375

COG function: function code R; Zn finger protein HypA/HybF (possibly regulating hydrogenase expression)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypA/hybF family

Homologues:

Organism=Escherichia coli, GI1789365, Length=113, Percent_Identity=84.9557522123894, Blast_Score=194, Evalue=9e-52,
Organism=Escherichia coli, GI1789081, Length=114, Percent_Identity=49.1228070175439, Blast_Score=126, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYBF_SALTI (P64422)

Other databases:

- EMBL:   AL627277
- EMBL:   AE014613
- RefSeq:   NP_457539.1
- RefSeq:   NP_806751.1
- GeneID:   1071566
- GeneID:   1249587
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t3065
- KEGG:   sty:STY3315
- HOGENOM:   HBG688671
- OMA:   MHEMSLC
- ProtClustDB:   PRK12380
- BioCyc:   SENT209261:T3065-MONOMER
- BioCyc:   SENT220341:STY3315-MONOMER
- HAMAP:   MF_00213
- InterPro:   IPR000688
- InterPro:   IPR020538
- TIGRFAMs:   TIGR00100

Pfam domain/function: PF01155 HypA

EC number: NA

Molecular weight: Translated: 12659; Mature: 12659

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS01249 HYPA; PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
7.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHELSLCQSAVEIIQQQAEQHGVARVTGVWLEIGALSCVEERAVRFSFDIACQGTLAQGC
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCC
ELHIDYRPAQAWCWDCSQVVEILRHDAQCPHCHGDRLRVDTGDSLKVKSIEVE
EEEEECCCCHHHHCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEEEEEECC
>Mature Secondary Structure
MHELSLCQSAVEIIQQQAEQHGVARVTGVWLEIGALSCVEERAVRFSFDIACQGTLAQGC
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCC
ELHIDYRPAQAWCWDCSQVVEILRHDAQCPHCHGDRLRVDTGDSLKVKSIEVE
EEEEECCCCHHHHCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504