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The map label for this gene is gcvH [H]

Identifier: 29143322

GI number: 29143322

Start: 3063190

End: 3063582

Strand: Reverse

Name: gcvH [H]

Synonym: t2972

Alternate gene names: 29143322

Gene position: 3063582-3063190 (Counterclockwise)

Preceding gene: 29143323

Following gene: 29143321

Centisome position: 63.93

GC content: 55.22

Gene sequence:

>393_bases
TTGATGAGCAACGTACCCGCAGAACTGAAATACAGCAAAGAACACGAATGGCTGCGTAAAGAAGCGGATGGCACTTACAC
CGTTGGCATTACTGAACACGCTCAGGAGCTGTTGGGCGATATGGTTTTTGTTGATCTGCCGGAAGTCGGCGCGACCGTAA
GCGCGGGCGACGACTGCGCCGTGGCGGAGTCCGTAAAAGCGGCGTCCGACATTTATGCGCCGGTAAGCGGCGAGATCGTG
GCGGTAAACGATGCGCTGAGCGACTCCCCGGAGCTGGTGAACAGCGAACCGTATGCCGGTGGCTGGATCTTTAAAATCAA
AGCCAGCGATGAAAGCGAGCTTGAATCGCTGCTGGATGCCACCGCGTATGAGGCATTGTTAGAAGACGAATAA

Upstream 100 bases:

>100_bases
GTACAAATTCGTAACCGTGAAATGCCGGTAAAAGTGACTAAACCTGTTTTTGTGCGTAACGGCAAAGCCGTCGCGTGATT
CATCCTTTTTTGGAGATTAA

Downstream 100 bases:

>100_bases
TGTTGTGCCGGATGGCGCTAATGCTTATCCGGCCTACTCACTGCAACGCGAATGATTTTGCGTATTCGTTTTCGCACGCG
TAGGCCCGGTAAGCGTCAGC

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 130; Mature: 130

Protein sequence:

>130_residues
MMSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIV
AVNDALSDSPELVNSEPYAGGWIFKIKASDESELESLLDATAYEALLEDE

Sequences:

>Translated_130_residues
MMSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIV
AVNDALSDSPELVNSEPYAGGWIFKIKASDESELESLLDATAYEALLEDE
>Mature_130_residues
MMSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIV
AVNDALSDSPELVNSEPYAGGWIFKIKASDESELESLLDATAYEALLEDE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=120, Percent_Identity=45, Blast_Score=113, Evalue=4e-26,
Organism=Homo sapiens, GI89057342, Length=120, Percent_Identity=44.1666666666667, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI1789271, Length=129, Percent_Identity=100, Blast_Score=257, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17507493, Length=120, Percent_Identity=46.6666666666667, Blast_Score=114, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17551294, Length=121, Percent_Identity=43.801652892562, Blast_Score=108, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6319272, Length=128, Percent_Identity=38.28125, Blast_Score=100, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17865652, Length=127, Percent_Identity=42.5196850393701, Blast_Score=115, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13942; Mature: 13942

Theoretical pI: Translated: 3.72; Mature: 3.72

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVSAGDDCA
CCCCCCCCCEECHHHHHHHHCCCCEEEEEHHHHHHHHHHCEEEEECCCCCCEECCCCCCH
VAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWIFKIKASDESELESLLDA
HHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHH
TAYEALLEDE
HHHHHHHCCC
>Mature Secondary Structure
MMSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVSAGDDCA
CCCCCCCCCEECHHHHHHHHCCCCEEEEEHHHHHHHHHHCEEEEECCCCCCEECCCCCCH
VAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWIFKIKASDESELESLLDA
HHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHH
TAYEALLEDE
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA