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The map label for this gene is ntcB [H]

Identifier: 29143282

GI number: 29143282

Start: 3023805

End: 3024668

Strand: Reverse

Name: ntcB [H]

Synonym: t2930

Alternate gene names: 29143282

Gene position: 3024668-3023805 (Counterclockwise)

Preceding gene: 29143285

Following gene: 29143281

Centisome position: 63.12

GC content: 55.09

Gene sequence:

>864_bases
ATGCGTTACTCACCTGAAGCGTTAATGGCATTTGTTGAGGCCGTCTCTTGCGGCTCGTTTTCTGCGGCGGCGCGCCGCCT
GCGTAAAAGCCAGTCCACCATTAGTACCGCCATTGCGCATCTGGAGGCGGATCTGGGATTTTCATTATTTGATCGATCAT
CGCGCCAGCCCGTTCTGACCGAAGAAGGAAAACGGGTGCTTAGCTATGTTCAGGCTATTTTATCGGCGAGCGACCGTCTG
GATGAGGTGGCGCTGACGTTATCCGGCGAGACGGAGACAAGGCTCACGTTCGTCCTCTCTGATACCCTGCACCCTGACAG
TCTGGAAGAGTTAATGGCGCAGTTTGATCGTCAATTCCCGCATACCGAGTTTGAATGTTTGATCGGAGAAAATGATGATG
TCATCGACCTGCTGCAAAAAGGGCGCGCCCAGATAGGCCTGATTGAAGCGCGTGATAACTATCCCACAGACATTGGCGCG
ACGCGTCTGCTTGTTCAAAGCTGGATGGGGGTTTATGTGGCGACCACGCATCCGTTGGCGTCGCAGACGAACCTGCAATG
GGAGCAACTGCATACCTGGCGCGAGCTGCGTCTGAATACCTACCTTGACTCTAACGCCAACATTACGCGCGGCCCGGCCT
GGTCAGCGCCAAACTATTTATTGCTGTTGAGCATGGCGGTTCAGGGATTTGGCTGGTGCGTATTGCCCTGCGCGCTGGTG
GAAGAGTTTGCGGCGGAAAAACCGCTGGCGCAGCTGGATGTTCCTGGCTGGCCGAGGGCGATTGCTATTGATTTGTTGTG
GAACAAGAAGACGCCGCCCGGAACGGCGGGGAACTGGCTTCGCTACCATTTACAGCAGAAATAA

Upstream 100 bases:

>100_bases
TGAAAAAACCTTACTCTTAATCAAATTAAAGAAATTTCCGCTATTTTATCGTTTAATATGATACCTAAAAGATAAAATCC
ATCGATAAAGTAGATAGGTT

Downstream 100 bases:

>100_bases
GCTGCGCCACCGTCAAATCAGCGTCATTTCCTTTGTGATAAAACTCACTTTTTTTAAACAATCAAGATAACTAATTTTAA
CAATGCTCTACTATGGCGGT

Product: regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MRYSPEALMAFVEAVSCGSFSAAARRLRKSQSTISTAIAHLEADLGFSLFDRSSRQPVLTEEGKRVLSYVQAILSASDRL
DEVALTLSGETETRLTFVLSDTLHPDSLEELMAQFDRQFPHTEFECLIGENDDVIDLLQKGRAQIGLIEARDNYPTDIGA
TRLLVQSWMGVYVATTHPLASQTNLQWEQLHTWRELRLNTYLDSNANITRGPAWSAPNYLLLLSMAVQGFGWCVLPCALV
EEFAAEKPLAQLDVPGWPRAIAIDLLWNKKTPPGTAGNWLRYHLQQK

Sequences:

>Translated_287_residues
MRYSPEALMAFVEAVSCGSFSAAARRLRKSQSTISTAIAHLEADLGFSLFDRSSRQPVLTEEGKRVLSYVQAILSASDRL
DEVALTLSGETETRLTFVLSDTLHPDSLEELMAQFDRQFPHTEFECLIGENDDVIDLLQKGRAQIGLIEARDNYPTDIGA
TRLLVQSWMGVYVATTHPLASQTNLQWEQLHTWRELRLNTYLDSNANITRGPAWSAPNYLLLLSMAVQGFGWCVLPCALV
EEFAAEKPLAQLDVPGWPRAIAIDLLWNKKTPPGTAGNWLRYHLQQK
>Mature_287_residues
MRYSPEALMAFVEAVSCGSFSAAARRLRKSQSTISTAIAHLEADLGFSLFDRSSRQPVLTEEGKRVLSYVQAILSASDRL
DEVALTLSGETETRLTFVLSDTLHPDSLEELMAQFDRQFPHTEFECLIGENDDVIDLLQKGRAQIGLIEARDNYPTDIGA
TRLLVQSWMGVYVATTHPLASQTNLQWEQLHTWRELRLNTYLDSNANITRGPAWSAPNYLLLLSMAVQGFGWCVLPCALV
EEFAAEKPLAQLDVPGWPRAIAIDLLWNKKTPPGTAGNWLRYHLQQK

Specific function: Seems to regulate utilization of fixed nitrogen by controlling the expression of a certain gene(s) involved in nitrogen metabolism [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786713, Length=281, Percent_Identity=25.6227758007117, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786401, Length=146, Percent_Identity=35.6164383561644, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082024, Length=274, Percent_Identity=26.6423357664234, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1788481, Length=184, Percent_Identity=27.1739130434783, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1786508, Length=295, Percent_Identity=24.7457627118644, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788296, Length=181, Percent_Identity=28.7292817679558, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790262, Length=237, Percent_Identity=26.5822784810127, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32135; Mature: 32135

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYSPEALMAFVEAVSCGSFSAAARRLRKSQSTISTAIAHLEADLGFSLFDRSSRQPVLT
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHH
EEGKRVLSYVQAILSASDRLDEVALTLSGETETRLTFVLSDTLHPDSLEELMAQFDRQFP
HHHHHHHHHHHHHHHHHHCHHHEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCC
HTEFECLIGENDDVIDLLQKGRAQIGLIEARDNYPTDIGATRLLVQSWMGVYVATTHPLA
CCCEEEEECCCCCHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCEEEEECCCCC
SQTNLQWEQLHTWRELRLNTYLDSNANITRGPAWSAPNYLLLLSMAVQGFGWCVLPCALV
CCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
EEFAAEKPLAQLDVPGWPRAIAIDLLWNKKTPPGTAGNWLRYHLQQK
HHHHHCCCCHHCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHEECCC
>Mature Secondary Structure
MRYSPEALMAFVEAVSCGSFSAAARRLRKSQSTISTAIAHLEADLGFSLFDRSSRQPVLT
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHH
EEGKRVLSYVQAILSASDRLDEVALTLSGETETRLTFVLSDTLHPDSLEELMAQFDRQFP
HHHHHHHHHHHHHHHHHHCHHHEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCC
HTEFECLIGENDDVIDLLQKGRAQIGLIEARDNYPTDIGATRLLVQSWMGVYVATTHPLA
CCCEEEEECCCCCHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCEEEEECCCCC
SQTNLQWEQLHTWRELRLNTYLDSNANITRGPAWSAPNYLLLLSMAVQGFGWCVLPCALV
CCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
EEFAAEKPLAQLDVPGWPRAIAIDLLWNKKTPPGTAGNWLRYHLQQK
HHHHHCCCCHHCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7814317 [H]