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The map label for this gene is ygdE [H]

Identifier: 29143242

GI number: 29143242

Start: 2974198

End: 2975298

Strand: Reverse

Name: ygdE [H]

Synonym: t2888

Alternate gene names: 29143242

Gene position: 2975298-2974198 (Counterclockwise)

Preceding gene: 29143243

Following gene: 29143236

Centisome position: 62.09

GC content: 53.22

Gene sequence:

>1101_bases
ATGAATAAAGTTGTCTTGCTCTGCCGTCCGGGGTTTGAAAAAGAGTGCGCTGCAGAAATTACCGATAAAGCGGGAAAACG
GGAAATATTTGGTTTTGCACGCGTAAAAGAGAACGCAGGCTATGTCATTTATGAATGCTACCAGCCGGAAGATGGCGAAA
AGCTGATTTCAGAACTGCCGTTCAGTTCGCTGATTTTCGCCCGCCAGTGGTTTGTGGTGGGTGAACTGCTGCAACATTTA
CCGCCGGAAGACAGAATCACGCCGATTGTCGGCATGTTGCAGGGCGTAGTGGAAAAGGGCGGCGAGCTGCGGGTTGAAGT
CGCCGACACCAATGAAAGTAAAGAACTGATGAAGTTCTGCCGCAAATTTACGGTGCCGCTGCGCGCGGCTTTGCGTGATG
CGGGCGTATTGACGAATTATGAAACGCCGAAGCGTCCGGTCGTTCATGTCTTTTTTATCGCGCCAGGATGCTGCTATACC
GGTTATTCGTTCGCGCATAACAATTCACCGTTTTATATGGGCATCCCGCGTTTGAAGTTTCCGTCTGATGCGCCGAGCCG
CTCAACGCTGAAACTGGAAGAGGCGCTCCACGTTTTCATCCCGGAAGATGAGTGGGATGAACGTCTGGCAAACGGCATGT
ACGCGGTGGATTTGGGCGCCTGTCCCGGCGGATGGACGTACCAGTTAGTGAAGCGCAATATGTGGGTCTACTCCGTAGAT
AACGGCCCGATGGCGCAAAGCCTGATGGACACCGGACAGGTGACCTGGTTGCGTGAGGATGGCTTCAGGTACCGTCCGAA
CCGGAATAATATTTCATGGATGGTCTGCGATATGGTGGAGAAGCCGGCCAAAGTCACCGCGTTAATGGCGCAGTGGCTGG
TGAACGGCTGGTGTCGGGAGACGATTTTTAACCTTAAACTGCCGATGAAAAAACGCTATGAAGAGGTATCGCATAACCTG
GCGTATCTTCAGGCGCAGTTGGATGAGCATGGCGTTAATGCGCAGATCCAGGCGCGTCAGCTTTATCACGATCGCGAAGA
AGTGACGGTACACGTGCGTCGGTTGTGGGCGGCTGTTGGCGGACGTCGCGACGAGCGATAA

Upstream 100 bases:

>100_bases
CTGTGGGCGTATATCACGCCTGTCGGCGGCGTCAGTTTCCTGGTGGGCTGGGCGCTATTGCTGATTGGCGCCATTCGTAT
GAAACGTAAGGGCGCGAGCC

Downstream 100 bases:

>100_bases
AAACGCGCTGCCGTCATCCGGCGCGTCATCAGCGCCGGATGGCGCACGCTTATCCGGCCTATGACTGCGGCGCATCTGAA
ACGATATGCGTCACATCATC

Product: RNA 2'-O-ribose methyltransferase

Products: NA

Alternate protein names: 23S rRNA 2'-O-ribose methyltransferase rlmM [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MNKVVLLCRPGFEKECAAEITDKAGKREIFGFARVKENAGYVIYECYQPEDGEKLISELPFSSLIFARQWFVVGELLQHL
PPEDRITPIVGMLQGVVEKGGELRVEVADTNESKELMKFCRKFTVPLRAALRDAGVLTNYETPKRPVVHVFFIAPGCCYT
GYSFAHNNSPFYMGIPRLKFPSDAPSRSTLKLEEALHVFIPEDEWDERLANGMYAVDLGACPGGWTYQLVKRNMWVYSVD
NGPMAQSLMDTGQVTWLREDGFRYRPNRNNISWMVCDMVEKPAKVTALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNL
AYLQAQLDEHGVNAQIQARQLYHDREEVTVHVRRLWAAVGGRRDER

Sequences:

>Translated_366_residues
MNKVVLLCRPGFEKECAAEITDKAGKREIFGFARVKENAGYVIYECYQPEDGEKLISELPFSSLIFARQWFVVGELLQHL
PPEDRITPIVGMLQGVVEKGGELRVEVADTNESKELMKFCRKFTVPLRAALRDAGVLTNYETPKRPVVHVFFIAPGCCYT
GYSFAHNNSPFYMGIPRLKFPSDAPSRSTLKLEEALHVFIPEDEWDERLANGMYAVDLGACPGGWTYQLVKRNMWVYSVD
NGPMAQSLMDTGQVTWLREDGFRYRPNRNNISWMVCDMVEKPAKVTALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNL
AYLQAQLDEHGVNAQIQARQLYHDREEVTVHVRRLWAAVGGRRDER
>Mature_366_residues
MNKVVLLCRPGFEKECAAEITDKAGKREIFGFARVKENAGYVIYECYQPEDGEKLISELPFSSLIFARQWFVVGELLQHL
PPEDRITPIVGMLQGVVEKGGELRVEVADTNESKELMKFCRKFTVPLRAALRDAGVLTNYETPKRPVVHVFFIAPGCCYT
GYSFAHNNSPFYMGIPRLKFPSDAPSRSTLKLEEALHVFIPEDEWDERLANGMYAVDLGACPGGWTYQLVKRNMWVYSVD
NGPMAQSLMDTGQVTWLREDGFRYRPNRNNISWMVCDMVEKPAKVTALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNL
AYLQAQLDEHGVNAQIQARQLYHDREEVTVHVRRLWAAVGGRRDER

Specific function: Catalyzes the 2'-O-methylation at nucleotide C2498 in 23S rRNA [H]

COG id: COG2933

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family. RlmM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789171, Length=366, Percent_Identity=94.2622950819672, Blast_Score=734, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011224
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: NA

Molecular weight: Translated: 42021; Mature: 42021

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVVLLCRPGFEKECAAEITDKAGKREIFGFARVKENAGYVIYECYQPEDGEKLISELP
CCCEEEEECCCCCHHHHHHHHHCCCCHHHHEEEEEECCCCEEEEEEECCCCHHHHHHHCC
FSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGELRVEVADTNESKELMKFC
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHH
RKFTVPLRAALRDAGVLTNYETPKRPVVHVFFIAPGCCYTGYSFAHNNSPFYMGIPRLKF
HHHCHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCHHCCCCEEECCCCEEECCCCCCC
PSDAPSRSTLKLEEALHVFIPEDEWDERLANGMYAVDLGACPGGWTYQLVKRNMWVYSVD
CCCCCCCCCEEEECCEEEECCCCHHHHHHHCCEEEEEECCCCCCCCHHEECCCEEEEEEC
NGPMAQSLMDTGQVTWLREDGFRYRPNRNNISWMVCDMVEKPAKVTALMAQWLVNGWCRE
CCHHHHHHHCCCCEEEEECCCCEECCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHH
TIFNLKLPMKKRYEEVSHNLAYLQAQLDEHGVNAQIQARQLYHDREEVTVHVRRLWAAVG
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHCCHHHHHHHHHHHHHHHC
GRRDER
CCCCCC
>Mature Secondary Structure
MNKVVLLCRPGFEKECAAEITDKAGKREIFGFARVKENAGYVIYECYQPEDGEKLISELP
CCCEEEEECCCCCHHHHHHHHHCCCCHHHHEEEEEECCCCEEEEEEECCCCHHHHHHHCC
FSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGELRVEVADTNESKELMKFC
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHH
RKFTVPLRAALRDAGVLTNYETPKRPVVHVFFIAPGCCYTGYSFAHNNSPFYMGIPRLKF
HHHCHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCHHCCCCEEECCCCEEECCCCCCC
PSDAPSRSTLKLEEALHVFIPEDEWDERLANGMYAVDLGACPGGWTYQLVKRNMWVYSVD
CCCCCCCCCEEEECCEEEECCCCHHHHHHHCCEEEEEECCCCCCCCHHEECCCEEEEEEC
NGPMAQSLMDTGQVTWLREDGFRYRPNRNNISWMVCDMVEKPAKVTALMAQWLVNGWCRE
CCHHHHHHHCCCCEEEEECCCCEECCCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHH
TIFNLKLPMKKRYEEVSHNLAYLQAQLDEHGVNAQIQARQLYHDREEVTVHVRRLWAAVG
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHCCHHHHHHHHHHHHHHHC
GRRDER
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA