Click here to switch to the map view.

The map label for this gene is mazG [H]

Identifier: 29143210

GI number: 29143210

Start: 2933456

End: 2934256

Strand: Reverse

Name: mazG [H]

Synonym: t2855

Alternate gene names: 29143210

Gene position: 2934256-2933456 (Counterclockwise)

Preceding gene: 29143218

Following gene: 29143209

Centisome position: 61.23

GC content: 52.68

Gene sequence:

>801_bases
ATGACAACTAACCATCAAATTGACCGTCTTCTCACCCTCATGCAGCGCCTGCGCGACCCGGAAAACGGCTGCCCGTGGGA
TAAAGAGCAGACCTTCGCCAGCATCGCGCCTTATACACTGGAAGAAACCTATGAAGTACTGGATGCGATTGCGCGTGAGG
ATTTTGACGATTTGCGCGGCGAACTGGGCGACCTGTTGTTCCAGGTGGTGTTTTACGCGCAGATGGCGCAGGAAGAAGGC
CGCTTCGATTTTAATGATATTTGCGCTGCCATCAGCGACAAGCTGGAGCGCCGCCATCCGCATGTTTTTGGCGAGCTTTC
CGCGGATAATAGCGAAGAAGCATTAGTTCGCTGGGAGCAAATCAAAACGGAAGAGCGCGCGCAAAAAGCGCAGCATTCCG
CGCTGGACGATATTCCGCGCAGCTTACCGGCCTTAATGCGCGCGCAAAAAATCCAGAAACGGTGTTCAAACGTGGGCTTC
GACTGGACGACGCTGGGGCCGGTCGTCGATAAGGTCTATGAAGAGATCGATGAGGTGATGTTCGAGGCGCGGCAGGCGGT
TGTTGATCAGGCTAAACTGGAAGAGGAAATGGGCGATTTGCTCTTTGCCACGGTCAACATGGCGCGTCATTTAGGTACGA
AAGCGGAGATCGCTTTGCAAAAAGCGAACGATAAATTTGAACGTCGTTTTCGCGAAGTTGAACGTATTGTCGCAGCCAGA
GGCCTGGAAATGACCGGGGTAGACCTGGAAACAATGGAAGAAGTTTGGCAGGAAGTAAAACGCCAGGAAATTGATCTCTA
A

Upstream 100 bases:

>100_bases
TATATTTTATTATATAGGCGTTGTCGTAGTGTCTCTGTGCGTGAATGGTTAAGAATAATACGTTACAATTGTCGCTAGTA
TTTACAACCGATTAAAGACC

Downstream 100 bases:

>100_bases
AGGTTTTAGGCGGTCAAGAGCGATTTGTGTGATTTTTTAAATAACAAGCGCTTGATTTACGTCAAAAACATTTACCTCAA
AGGGGCTATTTTCTCCCTCC

Product: nucleoside triphosphate pyrophosphohydrolase

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MTTNHQIDRLLTLMQRLRDPENGCPWDKEQTFASIAPYTLEETYEVLDAIAREDFDDLRGELGDLLFQVVFYAQMAQEEG
RFDFNDICAAISDKLERRHPHVFGELSADNSEEALVRWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCSNVGF
DWTTLGPVVDKVYEEIDEVMFEARQAVVDQAKLEEEMGDLLFATVNMARHLGTKAEIALQKANDKFERRFREVERIVAAR
GLEMTGVDLETMEEVWQEVKRQEIDL

Sequences:

>Translated_266_residues
MTTNHQIDRLLTLMQRLRDPENGCPWDKEQTFASIAPYTLEETYEVLDAIAREDFDDLRGELGDLLFQVVFYAQMAQEEG
RFDFNDICAAISDKLERRHPHVFGELSADNSEEALVRWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCSNVGF
DWTTLGPVVDKVYEEIDEVMFEARQAVVDQAKLEEEMGDLLFATVNMARHLGTKAEIALQKANDKFERRFREVERIVAAR
GLEMTGVDLETMEEVWQEVKRQEIDL
>Mature_265_residues
TTNHQIDRLLTLMQRLRDPENGCPWDKEQTFASIAPYTLEETYEVLDAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGR
FDFNDICAAISDKLERRHPHVFGELSADNSEEALVRWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCSNVGFD
WTTLGPVVDKVYEEIDEVMFEARQAVVDQAKLEEEMGDLLFATVNMARHLGTKAEIALQKANDKFERRFREVERIVAARG
LEMTGVDLETMEEVWQEVKRQEIDL

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=262, Percent_Identity=94.2748091603053, Blast_Score=514, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 30797; Mature: 30666

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNHQIDRLLTLMQRLRDPENGCPWDKEQTFASIAPYTLEETYEVLDAIAREDFDDLRG
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHH
ELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFGELSADNSEEALVRWEQ
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHH
IKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCSNVGFDWTTLGPVVDKVYEEIDEVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
FEARQAVVDQAKLEEEMGDLLFATVNMARHLGTKAEIALQKANDKFERRFREVERIVAAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLEMTGVDLETMEEVWQEVKRQEIDL
CCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTNHQIDRLLTLMQRLRDPENGCPWDKEQTFASIAPYTLEETYEVLDAIAREDFDDLRG
CCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHH
ELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFGELSADNSEEALVRWEQ
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHH
IKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCSNVGFDWTTLGPVVDKVYEEIDEVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
FEARQAVVDQAKLEEEMGDLLFATVNMARHLGTKAEIALQKANDKFERRFREVERIVAAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLEMTGVDLETMEEVWQEVKRQEIDL
CCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]