Click here to switch to the map view.

The map label for this gene is hycG [H]

Identifier: 29143108

GI number: 29143108

Start: 2831322

End: 2832089

Strand: Reverse

Name: hycG [H]

Synonym: t2749

Alternate gene names: 29143108

Gene position: 2832089-2831322 (Counterclockwise)

Preceding gene: 29143109

Following gene: 29143107

Centisome position: 59.1

GC content: 59.11

Gene sequence:

>768_bases
ATGAGCAATTTATTAGGCCCGCGTGACGCCAACGGCATTCCGGTACCGATGACGGTGGATGAATCCATCGCCAGCATGAA
GGCGTCTCTACTGAAAAATATTAAGCGTTCGGCATACGTTTACCGCGTGGACTGCGGCGGCTGTAACGGCTGTGAAATCG
AAATCTTTGCGACGCTTTCGCCGCTGTTTGATGCCGAGCGTTTCGGCATCAAAGTAGTACCGTCGCCGCGTCATGCCGAT
ATTCTGCTGTTTACCGGCGTGGTGACCCGCGCCATGCGCTCTCCGGCGCTGCGCGCCTGGCAGTCGGCGCCGGACCCGAA
AATTTGTATCTCTTACGGCGCCTGCGGCAACAGCGGCGGCATCTTCCATGATCTGTACTGCGTCTGGGGCGGTACCGACA
AAATCGTCCCGGTGGATGTGTACATTCCAGGCTGCCCGCCAACGCCTGCCGCCACGCTGTATGGCTTTGCAATGGCGCTT
GGCCTGCTGGAGCAGAAAATCCACGCCCGCGCGCCGGGTGAGCTGGACGATCAGCCCGCAGAGATCCTGCATCCGGATAT
GGTGCAGCCGCTGCGCGTGAAGGTGGATCGCGCGGCGTGCCGGCTGGCGGGTTATCGCTATGGTCGTCAGATCGCCGATG
ATTACCTGACACAGTTGGGGCAGGGCGAGCAGCAAGTGGCGCGTTGGCTGGAAGCGGAAAACGATCCGCGTTTAACCGAA
ATTGTCATCCATCTCAATCATGTTGTTGAAGAGGCGCGTATCCGATGA

Upstream 100 bases:

>100_bases
ACCGCGAAAGCTTTGAGACCTGCCCGGACTGTAAGCGTCAGAAATGCCTGGTGCCGTCCGACCGTATTGAACTGACTCGC
CATATGAAAGAGGTCAGCTA

Downstream 100 bases:

>100_bases
GCGAACAGGTGGTGTTCAGTCAACTGAGCCGTAAGTTTATTGATGAGAACGATGCGACGCCCGCCGAGGCGCAGCAGGTT
GTCTATTACAGCCTGGCGAT

Product: formate hydrogenlyase subunit 7

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSNLLGPRDANGIPVPMTVDESIASMKASLLKNIKRSAYVYRVDCGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHAD
ILLFTGVVTRAMRSPALRAWQSAPDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
GLLEQKIHARAPGELDDQPAEILHPDMVQPLRVKVDRAACRLAGYRYGRQIADDYLTQLGQGEQQVARWLEAENDPRLTE
IVIHLNHVVEEARIR

Sequences:

>Translated_255_residues
MSNLLGPRDANGIPVPMTVDESIASMKASLLKNIKRSAYVYRVDCGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHAD
ILLFTGVVTRAMRSPALRAWQSAPDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
GLLEQKIHARAPGELDDQPAEILHPDMVQPLRVKVDRAACRLAGYRYGRQIADDYLTQLGQGEQQVARWLEAENDPRLTE
IVIHLNHVVEEARIR
>Mature_254_residues
SNLLGPRDANGIPVPMTVDESIASMKASLLKNIKRSAYVYRVDCGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADI
LLFTGVVTRAMRSPALRAWQSAPDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMALG
LLEQKIHARAPGELDDQPAEILHPDMVQPLRVKVDRAACRLAGYRYGRQIADDYLTQLGQGEQQVARWLEAENDPRLTEI
VIHLNHVVEEARIR

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=111, Percent_Identity=44.1441441441441, Blast_Score=105, Evalue=3e-23,
Organism=Escherichia coli, GI1789074, Length=255, Percent_Identity=96.078431372549, Blast_Score=505, Evalue=1e-144,
Organism=Escherichia coli, GI1788834, Length=251, Percent_Identity=60.9561752988048, Blast_Score=301, Evalue=3e-83,
Organism=Escherichia coli, GI1788624, Length=155, Percent_Identity=36.1290322580645, Blast_Score=98, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17509685, Length=130, Percent_Identity=40, Blast_Score=103, Evalue=7e-23,
Organism=Drosophila melanogaster, GI18859983, Length=110, Percent_Identity=42.7272727272727, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24642371, Length=110, Percent_Identity=42.7272727272727, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24651058, Length=110, Percent_Identity=43.6363636363636, Blast_Score=102, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 27955; Mature: 27823

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLLGPRDANGIPVPMTVDESIASMKASLLKNIKRSAYVYRVDCGGCNGCEIEIFATLS
CCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCEEEEEEECC
PLFDAERFGIKVVPSPRHADILLFTGVVTRAMRSPALRAWQSAPDPKICISYGACGNSGG
HHHHHHHCCEEEECCCCCCCEEEHHHHHHHHHHCHHHHHHHCCCCCCEEEEECCCCCCCC
IFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMALGLLEQKIHARAPGELDDQPA
CEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
EILHPDMVQPLRVKVDRAACRLAGYRYGRQIADDYLTQLGQGEQQVARWLEAENDPRLTE
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHH
IVIHLNHVVEEARIR
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNLLGPRDANGIPVPMTVDESIASMKASLLKNIKRSAYVYRVDCGGCNGCEIEIFATLS
CCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCEEEEEEECC
PLFDAERFGIKVVPSPRHADILLFTGVVTRAMRSPALRAWQSAPDPKICISYGACGNSGG
HHHHHHHCCEEEECCCCCCCEEEHHHHHHHHHHCHHHHHHHCCCCCCEEEEECCCCCCCC
IFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMALGLLEQKIHARAPGELDDQPA
CEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
EILHPDMVQPLRVKVDRAACRLAGYRYGRQIADDYLTQLGQGEQQVARWLEAENDPRLTE
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHH
IVIHLNHVVEEARIR
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]