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The map label for this gene is iroD [H]

Identifier: 29143039

GI number: 29143039

Start: 2760268

End: 2761512

Strand: Direct

Name: iroD [H]

Synonym: t2670

Alternate gene names: 29143039

Gene position: 2760268-2761512 (Clockwise)

Preceding gene: 29143038

Following gene: 29143040

Centisome position: 57.6

GC content: 61.77

Gene sequence:

>1245_bases
ATGAGACTGAGCCCGCCCGTCGCTCCTGTCGCTATACAGACCGCTACGCGCCTTCGCCGTCAACTCGCCGCCGGAAGCCA
GGTTGACGCTTCTCACTTCTGGCGAGAAGCAAACTCGCTCGCGCTGCCGCTGGTGACAGCCATTAACGGCGCAGACGATG
AACGGGAAGTCACCTTCCTGTGGCGTGCCGCCTCTCCTCTGCGGGGGGTTTATGTTCGCCTGAACCGTGTCACCGATAAA
GATAACGTGGCGAAAGGTATGATGACGCAACTGCCCACAACGGATATCTGGCATCTGACGCTTCGCTTACCGGCGTCCTA
CTGCGGTTCCTATACGATGGTCGAAATACCACCGAAAACCCCGGACGAGACGGTGTTGCAGCTTGGAAGCCGTTTCGCCT
CCCTTGTCGGGAAGGCGGACCCACTGAACAGCACGCCGGGAATAAATGTTCGGGGCAATGCGCAGGAGTCCGTTCTGGCG
TTGGATCATGCCCCGGCACAGGAGGAGTGGTCAGGCTGTCGCGCTTACGCCGGGCAGCTTTTCACCTCAGAACATCGGCT
CGCCGGACAACGTCGCCGTGTGCGGCTGTATCTTCCTGACGTTCCTGTCGAACAACCGCTGGGCTTGCTGGTACTCACGG
ATGGCGAGATCTGGTTCGACCATCTTGGGGTAAGCGCCGCCATTGACGCCGCCATCCGCAGCGGACGCATCGCGCCGGTG
GCCGTACTCGGCGTTGATAATATCAATGCGCGTGAACGCGTCGCAATCCTCGGCGGTCGCCGCGAGCTGGTGCTCGACAT
TGCCGAACGCCTGCTGCCAACCCTTCGCGCCAAATACCCGGAACGACAGTGGGCGGACCGTACACAAACGGTGCTGGCAG
GGCAAAGCCTCGGCGGCGTGACCGCGCTGATGGCGGCCCGCCACGCGCCAGAGAGCTTCGGCCTGGTGCTGAGCCACTCC
CCCTCAATGTGGTGGACGCCGGACAACCGCAATCGCCCGCCCCATTTCAGCGCAGAAGAGCGCTCATGGGTCAGCGAACA
TGTGTTATCCGCGCCCTCGCCAGCCGTACGTACGCATCTGTGCGTGGGATCGCTGGAAGGTTCGACGGTCCCGCAGGTCA
AACAGTTGCATGAAAAATTGCGCGCCGCCGGTGTTGAAAGCCATTGCTGCGTGTACACCGGCGGTCACGATTATGCCTGG
TGGCGCGGCGCGCTGATTGACGGATTACGTCTACTGCCCCGATAA

Upstream 100 bases:

>100_bases
CCACGATTTACGCAAAAGAAAGCCCGCAAGCGGCTGTTCCTGTAACAGCCGCTTTAGCTATTGATACTGACCGAAATATA
AACGAGATGGAATACAAAGA

Downstream 100 bases:

>100_bases
AACTTCACCTGCCAAAAAGGAATAACATCCGATGTATGGTCGCCAGTACCACAACAAGTGCTATCGCGTGGCGCTATTTT
TAGTGAGTTTTTGTTTTAGC

Product: ferric enterochelin esterase

Products: Fe [C]

Alternate protein names: Ferric enterobactin esterase [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MRLSPPVAPVAIQTATRLRRQLAAGSQVDASHFWREANSLALPLVTAINGADDEREVTFLWRAASPLRGVYVRLNRVTDK
DNVAKGMMTQLPTTDIWHLTLRLPASYCGSYTMVEIPPKTPDETVLQLGSRFASLVGKADPLNSTPGINVRGNAQESVLA
LDHAPAQEEWSGCRAYAGQLFTSEHRLAGQRRRVRLYLPDVPVEQPLGLLVLTDGEIWFDHLGVSAAIDAAIRSGRIAPV
AVLGVDNINARERVAILGGRRELVLDIAERLLPTLRAKYPERQWADRTQTVLAGQSLGGVTALMAARHAPESFGLVLSHS
PSMWWTPDNRNRPPHFSAEERSWVSEHVLSAPSPAVRTHLCVGSLEGSTVPQVKQLHEKLRAAGVESHCCVYTGGHDYAW
WRGALIDGLRLLPR

Sequences:

>Translated_414_residues
MRLSPPVAPVAIQTATRLRRQLAAGSQVDASHFWREANSLALPLVTAINGADDEREVTFLWRAASPLRGVYVRLNRVTDK
DNVAKGMMTQLPTTDIWHLTLRLPASYCGSYTMVEIPPKTPDETVLQLGSRFASLVGKADPLNSTPGINVRGNAQESVLA
LDHAPAQEEWSGCRAYAGQLFTSEHRLAGQRRRVRLYLPDVPVEQPLGLLVLTDGEIWFDHLGVSAAIDAAIRSGRIAPV
AVLGVDNINARERVAILGGRRELVLDIAERLLPTLRAKYPERQWADRTQTVLAGQSLGGVTALMAARHAPESFGLVLSHS
PSMWWTPDNRNRPPHFSAEERSWVSEHVLSAPSPAVRTHLCVGSLEGSTVPQVKQLHEKLRAAGVESHCCVYTGGHDYAW
WRGALIDGLRLLPR
>Mature_414_residues
MRLSPPVAPVAIQTATRLRRQLAAGSQVDASHFWREANSLALPLVTAINGADDEREVTFLWRAASPLRGVYVRLNRVTDK
DNVAKGMMTQLPTTDIWHLTLRLPASYCGSYTMVEIPPKTPDETVLQLGSRFASLVGKADPLNSTPGINVRGNAQESVLA
LDHAPAQEEWSGCRAYAGQLFTSEHRLAGQRRRVRLYLPDVPVEQPLGLLVLTDGEIWFDHLGVSAAIDAAIRSGRIAPV
AVLGVDNINARERVAILGGRRELVLDIAERLLPTLRAKYPERQWADRTQTVLAGQSLGGVTALMAARHAPESFGLVLSHS
PSMWWTPDNRNRPPHFSAEERSWVSEHVLSAPSPAVRTHLCVGSLEGSTVPQVKQLHEKLRAAGVESHCCVYTGGHDYAW
WRGALIDGLRLLPR

Specific function: Upon internalization, ferric enterobactin is processed via an exquisitely specific pathway that is dependent on FES activity, making iron available for metabolic use [H]

COG id: COG2382

COG function: function code P; Enterochelin esterase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fes family [H]

Homologues:

Organism=Escherichia coli, GI1786799, Length=374, Percent_Identity=30.4812834224599, Blast_Score=125, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021764
- InterPro:   IPR000801
- InterPro:   IPR014756 [H]

Pfam domain/function: PF11806 DUF3327; PF00756 Esterase [H]

EC number: NA

Molecular weight: Translated: 45474; Mature: 45474

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSPPVAPVAIQTATRLRRQLAAGSQVDASHFWREANSLALPLVTAINGADDEREVTFL
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEHHEEECCCCCCCEEEEE
WRAASPLRGVYVRLNRVTDKDNVAKGMMTQLPTTDIWHLTLRLPASYCGSYTMVEIPPKT
EECCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEECCHHHCCCEEEEEECCCC
PDETVLQLGSRFASLVGKADPLNSTPGINVRGNAQESVLALDHAPAQEEWSGCRAYAGQL
CHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCEEEECCCCCHHHHHHHHHHHHHH
FTSEHRLAGQRRRVRLYLPDVPVEQPLGLLVLTDGEIWFDHLGVSAAIDAAIRSGRIAPV
HCCHHHHCCCCCEEEEEECCCCCCCCCCEEEEECCCEEEHHCCHHHHHHHHHHCCCCCEE
AVLGVDNINARERVAILGGRRELVLDIAERLLPTLRAKYPERQWADRTQTVLAGQSLGGV
EEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHH
TALMAARHAPESFGLVLSHSPSMWWTPDNRNRPPHFSAEERSWVSEHVLSAPSPAVRTHL
HHHHHHHCCCHHCCEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHEE
CVGSLEGSTVPQVKQLHEKLRAAGVESHCCVYTGGHDYAWWRGALIDGLRLLPR
EEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLSPPVAPVAIQTATRLRRQLAAGSQVDASHFWREANSLALPLVTAINGADDEREVTFL
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEHHEEECCCCCCCEEEEE
WRAASPLRGVYVRLNRVTDKDNVAKGMMTQLPTTDIWHLTLRLPASYCGSYTMVEIPPKT
EECCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEECCHHHCCCEEEEEECCCC
PDETVLQLGSRFASLVGKADPLNSTPGINVRGNAQESVLALDHAPAQEEWSGCRAYAGQL
CHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCEEEECCCCCHHHHHHHHHHHHHH
FTSEHRLAGQRRRVRLYLPDVPVEQPLGLLVLTDGEIWFDHLGVSAAIDAAIRSGRIAPV
HCCHHHHCCCCCEEEEEECCCCCCCCCCEEEEECCCEEEHHCCHHHHHHHHHHCCCCCEE
AVLGVDNINARERVAILGGRRELVLDIAERLLPTLRAKYPERQWADRTQTVLAGQSLGGV
EEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHH
TALMAARHAPESFGLVLSHSPSMWWTPDNRNRPPHFSAEERSWVSEHVLSAPSPAVRTHL
HHHHHHHCCCHHCCEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHEE
CVGSLEGSTVPQVKQLHEKLRAAGVESHCCVYTGGHDYAWWRGALIDGLRLLPR
EEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ferrienterobactin [C]

Specific reaction: ferrienterobactin = Fe [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA