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The map label for this gene is prfH [H]

Identifier: 29142917

GI number: 29142917

Start: 2610992

End: 2611606

Strand: Reverse

Name: prfH [H]

Synonym: t2535

Alternate gene names: 29142917

Gene position: 2611606-2610992 (Counterclockwise)

Preceding gene: 29142918

Following gene: 29142915

Centisome position: 54.5

GC content: 55.61

Gene sequence:

>615_bases
ATGATCTTACTGCAACTTTCTTCTGCGCAGGGACCGGACGAGTGTTGTCTGGCGGTAAAAAAAGCGCTGGATTGTCTGAC
GAAAGAAGCCGCCAGAGAAAAGGTATCGCTGACACGGCTTGAAACAGAGCCGGGACGATTACCAGACACGCTGCGTTCTG
CGCTGGTCTCGCTGGACGGCGAAAAAGCCATGGCTTTCAGTGAACGTTGGTGCGGCACGTTACTGTGGATTTGTACCAGC
CCGTATCGCCCGCACCATGGGCGAAAAAACTGGTACGTCGGCATTGGGCGCTTTTCCGCCGACGAGCACATACAGTCTGA
TGAGATTCGTTTCGAGACTCTGCGCTCTTCCGGCCCGGGTGGGCAGCACGTTAATAAAACCGACTCGGCAGTGCGGGCCA
CGCACCTGGCGAGCGGGATTAGCGTGAAGGTTCAGTCGGAGCGCAGTCAACATGCGAATAAGCGTCTGGCCCGGCTGCTG
ATTGCCTGGCGGCTGGAACAGCAACGGCAAAATGAGTGTGCGGCGTTGAAGTCTGAAAGGCGTCTGTTCCATCATCAGAT
TGAGCGCGGCAATCCGCTACGGATATTTAAAGGAATGGCGTTTACGCCACAATAA

Upstream 100 bases:

>100_bases
TCGATAGCGTTGTGGATTGTCTGGCAGACGCCGGACTGATTACGCCAGTTGCCTGCCTGCGTCCGGTGCTGACGCTGAAA
ACCTCAGGGGAGAAAAGCGC

Downstream 100 bases:

>100_bases
TTCGGTGCTACCAGCGCTGTTATAGGGCTGTCTGGCTATTCGCGTGTAGCCGCAGCGCTATTCAGCGCATCCAGGAGAGC
TTATCTGGCAATGGATACCC

Product: peptide chain release factor-like protein

Products: NA

Alternate protein names: RF-H [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MILLQLSSAQGPDECCLAVKKALDCLTKEAAREKVSLTRLETEPGRLPDTLRSALVSLDGEKAMAFSERWCGTLLWICTS
PYRPHHGRKNWYVGIGRFSADEHIQSDEIRFETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
IAWRLEQQRQNECAALKSERRLFHHQIERGNPLRIFKGMAFTPQ

Sequences:

>Translated_204_residues
MILLQLSSAQGPDECCLAVKKALDCLTKEAAREKVSLTRLETEPGRLPDTLRSALVSLDGEKAMAFSERWCGTLLWICTS
PYRPHHGRKNWYVGIGRFSADEHIQSDEIRFETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
IAWRLEQQRQNECAALKSERRLFHHQIERGNPLRIFKGMAFTPQ
>Mature_204_residues
MILLQLSSAQGPDECCLAVKKALDCLTKEAAREKVSLTRLETEPGRLPDTLRSALVSLDGEKAMAFSERWCGTLLWICTS
PYRPHHGRKNWYVGIGRFSADEHIQSDEIRFETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
IAWRLEQQRQNECAALKSERRLFHHQIERGNPLRIFKGMAFTPQ

Specific function: Could be involved in translational termination [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI34577120, Length=69, Percent_Identity=47.8260869565217, Blast_Score=68, Evalue=6e-12,
Organism=Escherichia coli, GI1787462, Length=62, Percent_Identity=54.8387096774194, Blast_Score=71, Evalue=4e-14,
Organism=Escherichia coli, GI2367172, Length=129, Percent_Identity=31.0077519379845, Blast_Score=66, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321295, Length=58, Percent_Identity=60.3448275862069, Blast_Score=72, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19921226, Length=75, Percent_Identity=50.6666666666667, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000352
- InterPro:   IPR017509 [H]

Pfam domain/function: PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 23042; Mature: 23042

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILLQLSSAQGPDECCLAVKKALDCLTKEAAREKVSLTRLETEPGRLPDTLRSALVSLDG
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHCCC
EKAMAFSERWCGTLLWICTSPYRPHHGRKNWYVGIGRFSADEHIQSDEIRFETLRSSGPG
CHHHHHHHHHHHHHHEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCC
GQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLLIAWRLEQQRQNECAALKSER
CCCCCCCHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLFHHQIERGNPLRIFKGMAFTPQ
HHHHHHHCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MILLQLSSAQGPDECCLAVKKALDCLTKEAAREKVSLTRLETEPGRLPDTLRSALVSLDG
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHCCC
EKAMAFSERWCGTLLWICTSPYRPHHGRKNWYVGIGRFSADEHIQSDEIRFETLRSSGPG
CHHHHHHHHHHHHHHEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCC
GQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLLIAWRLEQQRQNECAALKSER
CCCCCCCHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLFHHQIERGNPLRIFKGMAFTPQ
HHHHHHHCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1695895; 9278503; 1408743 [H]