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The map label for this gene is sbmA [H]

Identifier: 29142874

GI number: 29142874

Start: 2558021

End: 2559241

Strand: Reverse

Name: sbmA [H]

Synonym: t2488

Alternate gene names: 29142874

Gene position: 2559241-2558021 (Counterclockwise)

Preceding gene: 29142876

Following gene: 29142873

Centisome position: 53.41

GC content: 51.27

Gene sequence:

>1221_bases
ATGTTTAAATCTTTTTTCCCAAAGCCAGGGCCGTTTTTCATGTCGGCGTTTGTTTGGGCGCTGATCGCTGTCATTTTCTG
GCAGGCGGGAGGAGGAGATTGGGTGGCGCGACTTGTAGGGGCATCCGATGAAGTGCCTATCAGCGCAGCGCGCTTCTGGT
TGCTGGACTATCTGATTTTTTATGCTTATTACCTGATTTGCGTGGGATTATTTGCAACGTTCTGGTTCATCTATAGCCCG
CACCGCTGGCAGTACTGGTCGATACTTGGCACCTCGCTCATTATTTTTGTCACCTGGTTTTTGGTGGAGGTTGGCGTCGC
GGTTAACGCCTGGTACGCGCCATTCTATGATTTGATTCAAACGGCGCTCAACTCGCCGCACAAGGTCACTCTTGGTCAAT
TTTATCATGAAGTGGGGGTGTTTCTCGGTATTGCGCTTATCGCCGTGGTGATCGGCGTGCTGAACAACTTCTTTGTCAGC
CACTATGTATTTCGCTGGCGTACCGCAATGAATGAGCACTATATGGCGCACTGGCAGTACCTGCGCCATATCGAAGGGGC
CGCCCAGCGTGTGCAGGAAGATACCATGCGTTTTGCCTCTACACTGGAGAACATGGGCGTGAGTTTTATCAATGCCATCA
TGACGCTTATCGCCTTTCTGCCGGTGTTGGTCACCCTGTCAGCGCATGTGCCGAATTTACCCATCGTAGGACATATCCCT
TACGGCCTGGTCATTGCCGCCATAGTCTGGTCATTAATGGGGACGGGCCTGCTGGCGGTCGTCGGGATCAAATTGTCGGG
GCTGGAATTTAAAAATCAGCGAGTGGAGGCAGCTTATCGTAAAGAGCTGGTCTACGGTGAAGATGACGCCAGCCGCGCCA
CGCCGCCCACCGTCCGTGAACTGTTTAGCGCGGTGCGCCATAACTACTTCCGTCTCTACTTTCACTATATGTATTTCAAC
ATCGCGCGCATTCTCTATTTGCAGGTCGATAACGTTTTCGGTTTGTTCCTGCTGTTTCCTTCCATTGTTGCCGGTACGAT
TACGCTGGGGCTGATGACGCAAATCACCAACGTGTTTGGTCAGGTGCGCGGTTCGTTCCAGTATTTGATTAACTCCTGGA
CGACTCTGGTGGAGCTGATGTCTATTTATAAACGTTTACGTAGCTTTGAACGCCAGTTGGATGGTCAGCCAGTTCAGGAA
GTGACCCACTCTTTTAGCTAA

Upstream 100 bases:

>100_bases
ACAACGTAGGTCACAATGCCTGGTTTAGCCGTGGGCATGGCGTGCTATTGCCGCCGTCACAGCAGCCGTAATAAAGATAA
GAAGTTAGCAGGAGAGCATG

Downstream 100 bases:

>100_bases
CAGAAGGAAACGTTATGTCGGCGGCGTCCCGTCTTTATCCTCTGCCTTTTCTGGCGGTCGCCATATTGGCTGGCTGTAGT
AGTCAATCCGGGCAGCCGGT

Product: transport protein

Products: ADP; phosphate; microcin B17 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MFKSFFPKPGPFFMSAFVWALIAVIFWQAGGGDWVARLVGASDEVPISAARFWLLDYLIFYAYYLICVGLFATFWFIYSP
HRWQYWSILGTSLIIFVTWFLVEVGVAVNAWYAPFYDLIQTALNSPHKVTLGQFYHEVGVFLGIALIAVVIGVLNNFFVS
HYVFRWRTAMNEHYMAHWQYLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIAFLPVLVTLSAHVPNLPIVGHIP
YGLVIAAIVWSLMGTGLLAVVGIKLSGLEFKNQRVEAAYRKELVYGEDDASRATPPTVRELFSAVRHNYFRLYFHYMYFN
IARILYLQVDNVFGLFLLFPSIVAGTITLGLMTQITNVFGQVRGSFQYLINSWTTLVELMSIYKRLRSFERQLDGQPVQE
VTHSFS

Sequences:

>Translated_406_residues
MFKSFFPKPGPFFMSAFVWALIAVIFWQAGGGDWVARLVGASDEVPISAARFWLLDYLIFYAYYLICVGLFATFWFIYSP
HRWQYWSILGTSLIIFVTWFLVEVGVAVNAWYAPFYDLIQTALNSPHKVTLGQFYHEVGVFLGIALIAVVIGVLNNFFVS
HYVFRWRTAMNEHYMAHWQYLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIAFLPVLVTLSAHVPNLPIVGHIP
YGLVIAAIVWSLMGTGLLAVVGIKLSGLEFKNQRVEAAYRKELVYGEDDASRATPPTVRELFSAVRHNYFRLYFHYMYFN
IARILYLQVDNVFGLFLLFPSIVAGTITLGLMTQITNVFGQVRGSFQYLINSWTTLVELMSIYKRLRSFERQLDGQPVQE
VTHSFS
>Mature_406_residues
MFKSFFPKPGPFFMSAFVWALIAVIFWQAGGGDWVARLVGASDEVPISAARFWLLDYLIFYAYYLICVGLFATFWFIYSP
HRWQYWSILGTSLIIFVTWFLVEVGVAVNAWYAPFYDLIQTALNSPHKVTLGQFYHEVGVFLGIALIAVVIGVLNNFFVS
HYVFRWRTAMNEHYMAHWQYLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIAFLPVLVTLSAHVPNLPIVGHIP
YGLVIAAIVWSLMGTGLLAVVGIKLSGLEFKNQRVEAAYRKELVYGEDDASRATPPTVRELFSAVRHNYFRLYFHYMYFN
IARILYLQVDNVFGLFLLFPSIVAGTITLGLMTQITNVFGQVRGSFQYLINSWTTLVELMSIYKRLRSFERQLDGQPVQE
VTHSFS

Specific function: Required for the uptake of the peptide antibiotic microcin B17 (MccB17) [H]

COG id: COG1133

COG function: function code I; ABC-type long-chain fatty acid transport system, fused permease and ATPase components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To R.meliloti BacA [H]

Homologues:

Organism=Escherichia coli, GI1786575, Length=406, Percent_Identity=91.6256157635468, Blast_Score=765, Evalue=0.0,
Organism=Escherichia coli, GI1787772, Length=326, Percent_Identity=24.8466257668712, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011527
- InterPro:   IPR009248 [H]

Pfam domain/function: PF05992 SbmA_BacA [H]

EC number: NA

Molecular weight: Translated: 46493; Mature: 46493

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKSFFPKPGPFFMSAFVWALIAVIFWQAGGGDWVARLVGASDEVPISAARFWLLDYLIF
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
YAYYLICVGLFATFWFIYSPHRWQYWSILGTSLIIFVTWFLVEVGVAVNAWYAPFYDLIQ
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TALNSPHKVTLGQFYHEVGVFLGIALIAVVIGVLNNFFVSHYVFRWRTAMNEHYMAHWQY
HHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIAFLPVLVTLSAHVPNLPIVGHIP
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCH
YGLVIAAIVWSLMGTGLLAVVGIKLSGLEFKNQRVEAAYRKELVYGEDDASRATPPTVRE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
LFSAVRHNYFRLYFHYMYFNIARILYLQVDNVFGLFLLFPSIVAGTITLGLMTQITNVFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVRGSFQYLINSWTTLVELMSIYKRLRSFERQLDGQPVQEVTHSFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; microcin B17 [Periplasm]; H2O [C]

Specific reaction: ATP + microcin B17 [Periplasm] + H2O = ADP + phosphate + microcin B17 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]