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The map label for this gene is mdtG [H]

Identifier: 29142868

GI number: 29142868

Start: 2552804

End: 2554003

Strand: Reverse

Name: mdtG [H]

Synonym: t2482

Alternate gene names: 29142868

Gene position: 2554003-2552804 (Counterclockwise)

Preceding gene: 29142869

Following gene: 29142867

Centisome position: 53.3

GC content: 52.25

Gene sequence:

>1200_bases
ATGGAATCCTGGAAAGTTAATCTAATTTCCGTTTGGTTTGGATGTTTTTTTACCGGGCTGGCAATCAGCCAAATCCTGCC
ATTTTTACCCCTGTATATTTCCCAGCTTGGCGTCTCTTCCCATGAAGCGTTATCAATGTGGTCCGGGTTAACGTTTAGCA
TCACGTTTCTTATTTCCGCCATTGTATCGCCGATGTGGGGCAGTCTCGCCGATCGTAAAGGGCGTAAACTGATGCTATTG
CGCGCGTCGCTCGGGATGGCGATAGCTATTCTACTGCAAGCATTCGCGACCCATGTCTGGCAACTTTTCCTGCTGCGCGG
AATCATGGGGTTAACGTCAGGCTATATCCCCAATGCCATGGCGCTGGTAGCCTCTCAGGTACCGCGCGAACGTAGCGGCT
GGGCGCTCAGTACGCTTTCTACCGCGCAGATCAGCGGCGTTATCGGCGGGCCGTTAATGGGCGGCTTTGTCGCGGATCAT
ATCGGGCTGCGGGCGGTATTTCTGATTACCGCCATGCTGTTGGTGGTGAGCTTTCTGGTCACGCTATTTTTAATTAAAGA
AGGCGTGCGTCCGGTCATCAAGAAAAGCGAACGCCTGAGCGGTAAAGCCGTTTTTGCGTCGTTACCTTATCCTGCGCTGG
TGATCAGTTTGTTTTTTACCACGATGGTCATTCAACTCTGTAATGGTTCCATCAGTCCAATCCTGGCGCTGTTTATCAAA
TCAATGATGCCGGACAGTAATAACATCGCCTTTCTTAGCGGGTTAATCGCCTCGGTGCCCGGTATCTCCGCGCCTCGCCT
GGGAAAACTTGGCGACAGAATCGGCACGGAAAGAATTCTGATGGCCACGCTTATCTGCGCAGTGCTGCTTTTCTTCGCGA
TGTCCTGGGTCACCACGCCGTTCCAGCTGGGGCTGTTACGTTTCTTGTTAGGCTTTGCCGATGGCGCGATGTTACCCGCC
GTACAAACGTTATTGGTGAAATACTCCAGCGATCAAATTACCGGACGTATTTTTGGCTACAACCAGTCATTTATGTACCT
GGGCAACGTGGTTGGGCCGTTGATGGGCGCGACGGTATCGGCGATGGCCGGTTTCCGCTGGGTTTTTATCGCTACGGCGG
CGATCGTGTTGATCAATATTGGACAACTGACCCTGGCGTTACGTCATCGGCGTAACGCGCAAAAAGCGAAAGGCCAATAG

Upstream 100 bases:

>100_bases
AAAATAGCAAAGTGTGACGTATCGCACCTCTGGAACCACCAGGAAAATGAGAGCTTGCTAAGCTAACGCGTATTTTCAGC
TACTTACCGTTACGAGTCTT

Downstream 100 bases:

>100_bases
CGGTGCATCGTAACGGCGCTTCTGCCGTTACGATATGGTTTAAATTAAAAGATATACTCTTTATCCGTGAAGTTTTTCTA
TAGATGGCATAACCTTAAAT

Product: efflux pump

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MESWKVNLISVWFGCFFTGLAISQILPFLPLYISQLGVSSHEALSMWSGLTFSITFLISAIVSPMWGSLADRKGRKLMLL
RASLGMAIAILLQAFATHVWQLFLLRGIMGLTSGYIPNAMALVASQVPRERSGWALSTLSTAQISGVIGGPLMGGFVADH
IGLRAVFLITAMLLVVSFLVTLFLIKEGVRPVIKKSERLSGKAVFASLPYPALVISLFFTTMVIQLCNGSISPILALFIK
SMMPDSNNIAFLSGLIASVPGISAPRLGKLGDRIGTERILMATLICAVLLFFAMSWVTTPFQLGLLRFLLGFADGAMLPA
VQTLLVKYSSDQITGRIFGYNQSFMYLGNVVGPLMGATVSAMAGFRWVFIATAAIVLINIGQLTLALRHRRNAQKAKGQ

Sequences:

>Translated_399_residues
MESWKVNLISVWFGCFFTGLAISQILPFLPLYISQLGVSSHEALSMWSGLTFSITFLISAIVSPMWGSLADRKGRKLMLL
RASLGMAIAILLQAFATHVWQLFLLRGIMGLTSGYIPNAMALVASQVPRERSGWALSTLSTAQISGVIGGPLMGGFVADH
IGLRAVFLITAMLLVVSFLVTLFLIKEGVRPVIKKSERLSGKAVFASLPYPALVISLFFTTMVIQLCNGSISPILALFIK
SMMPDSNNIAFLSGLIASVPGISAPRLGKLGDRIGTERILMATLICAVLLFFAMSWVTTPFQLGLLRFLLGFADGAMLPA
VQTLLVKYSSDQITGRIFGYNQSFMYLGNVVGPLMGATVSAMAGFRWVFIATAAIVLINIGQLTLALRHRRNAQKAKGQ
>Mature_399_residues
MESWKVNLISVWFGCFFTGLAISQILPFLPLYISQLGVSSHEALSMWSGLTFSITFLISAIVSPMWGSLADRKGRKLMLL
RASLGMAIAILLQAFATHVWQLFLLRGIMGLTSGYIPNAMALVASQVPRERSGWALSTLSTAQISGVIGGPLMGGFVADH
IGLRAVFLITAMLLVVSFLVTLFLIKEGVRPVIKKSERLSGKAVFASLPYPALVISLFFTTMVIQLCNGSISPILALFIK
SMMPDSNNIAFLSGLIASVPGISAPRLGKLGDRIGTERILMATLICAVLLFFAMSWVTTPFQLGLLRFLLGFADGAMLPA
VQTLLVKYSSDQITGRIFGYNQSFMYLGNVVGPLMGATVSAMAGFRWVFIATAAIVLINIGQLTLALRHRRNAQKAKGQ

Specific function: Confers resistance to fosfomycin and deoxycholate [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. MdtG (TC 2.A.1.2.20) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787291, Length=396, Percent_Identity=59.5959595959596, Blast_Score=448, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43118; Mature: 43118

Theoretical pI: Translated: 11.09; Mature: 11.09

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESWKVNLISVWFGCFFTGLAISQILPFLPLYISQLGVSSHEALSMWSGLTFSITFLISA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHH
IVSPMWGSLADRKGRKLMLLRASLGMAIAILLQAFATHVWQLFLLRGIMGLTSGYIPNAM
HHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
ALVASQVPRERSGWALSTLSTAQISGVIGGPLMGGFVADHIGLRAVFLITAMLLVVSFLV
HHHHHHCCCCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLFLIKEGVRPVIKKSERLSGKAVFASLPYPALVISLFFTTMVIQLCNGSISPILALFIK
HHHHHHHCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SMMPDSNNIAFLSGLIASVPGISAPRLGKLGDRIGTERILMATLICAVLLFFAMSWVTTP
HHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
FQLGLLRFLLGFADGAMLPAVQTLLVKYSSDQITGRIFGYNQSFMYLGNVVGPLMGATVS
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
AMAGFRWVFIATAAIVLINIGQLTLALRHRRNAQKAKGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure
MESWKVNLISVWFGCFFTGLAISQILPFLPLYISQLGVSSHEALSMWSGLTFSITFLISA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHH
IVSPMWGSLADRKGRKLMLLRASLGMAIAILLQAFATHVWQLFLLRGIMGLTSGYIPNAM
HHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
ALVASQVPRERSGWALSTLSTAQISGVIGGPLMGGFVADHIGLRAVFLITAMLLVVSFLV
HHHHHHCCCCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLFLIKEGVRPVIKKSERLSGKAVFASLPYPALVISLFFTTMVIQLCNGSISPILALFIK
HHHHHHHCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SMMPDSNNIAFLSGLIASVPGISAPRLGKLGDRIGTERILMATLICAVLLFFAMSWVTTP
HHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
FQLGLLRFLLGFADGAMLPAVQTLLVKYSSDQITGRIFGYNQSFMYLGNVVGPLMGATVS
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
AMAGFRWVFIATAAIVLINIGQLTLALRHRRNAQKAKGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA