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The map label for this gene is acrA [H]

Identifier: 29142772

GI number: 29142772

Start: 2454627

End: 2455820

Strand: Direct

Name: acrA [H]

Synonym: t2384

Alternate gene names: 29142772

Gene position: 2454627-2455820 (Clockwise)

Preceding gene: 29142769

Following gene: 29142773

Centisome position: 51.22

GC content: 55.36

Gene sequence:

>1194_bases
ATGAACAAAAACAGAGGGTTAACGCCTCTGGCGGTCGTTCTGATGCTCTCAGGCAGCTTAGCGCTAACAGGATGTGACGA
CAAACAGGACCAGCAAGGCGGCCAGCAGATGCCAGAAGTTGGGGTTGTCACACTAAAAACGGAACCACTGCAAATCACAA
CTGAACTTCCGGGTCGTACCGTTGCTTACCGTATCGCCGAAGTTCGCCCGCAGGTAAGCGGCATTATCCTGAAGCGTAAT
TTCGTTGAGGGAAGTGATATCGAAGCGGGAGTCTCTCTCTATCAGATTGATCCTGCGACTTACCAGGCGACTTACGACAG
CGCTAAGGGCGATCTGGCAAAAGCGCAGGCCGCCGCGAATATCGCTGAACTGACGGTGAAGCGTTATCAAAAGCTGCTGG
GTACGCAGTACATCAGTAAGCAGGAATACGATCAGGCGCTGGCTGACGCGCAACAAGCGACTGCCGCCGTTGTCGCAGCA
AAAGCCGCCGTTGAAACCGCACGTATCAACCTGGCGTATACCAAAGTCACCTCACCGATTAGCGGTCGTATTGGTAAGTC
GTCCGTAACGGAAGGCGCGCTGGTACAGAACGGTCAGGCGTCGGCGCTGGCGACAGTGCAGCAGCTGGACCCTATTTATG
TCGATGTGACCCAGTCCAGCAATGACTTCCTGCGCCTGAAGCAGGAGCTGGCAAATGGTTCGCTGAAGCAGGAAAACGGC
AAAGCGAAGGTCGATCTGGTGACAAGCGACGGTATCAAATTCCCGCAGTCCGGTACGCTTGAATTCTCCGACGTGACCGT
TGACCAAAGCACCGGGTCTATTACTTTGCGCGCCATCTTCCCTAACCCGGATCACACCTTATTGCCAGGAATGTTCGTTC
GCGCACGTCTGCAGGAAGGGACAAAACCGACGGCATTACTGGTTCCACAACAGGGCGTTACCCGTACTCCACGCGGCGAT
GCCACGGTGCTGGTGGTTGGCGCTGATAACAAAGTGGAAACCCGCCAAATCGTCGCAAGCCAGGCGATCGGCGATAAGTG
GCTGGTGACTGACGGGTTGAAAGCGGGCGACCGCGTAGTCGTCAGCGGGCTGCAAAAAGTACGTCCTGGCGCACAGGTTA
AAGTGCAGGAAATTACCGCGGATAACAAACAGCAAGCCGCAAGCGGTGATCAACCTGCTCAGCCCAGGTCTTAA

Upstream 100 bases:

>100_bases
GGATGTATGTACCATAGCATGACGATAATATAAACGCAGCAATGGGTTTAAGGACCTTTGACCATTGACCAATTTGAAAT
CGGACACTCGAGGTTTACAT

Downstream 100 bases:

>100_bases
CTTAAACAGGAGCCGTTAAGACATGCCTAATTTCTTTATCGATCGCCCTATATTTGCGTGGGTGATCGCCATCATCATCA
TGTTGGCAGGGGGGCTCGCG

Product: acriflavin resistance protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MNKNRGLTPLAVVLMLSGSLALTGCDDKQDQQGGQQMPEVGVVTLKTEPLQITTELPGRTVAYRIAEVRPQVSGIILKRN
FVEGSDIEAGVSLYQIDPATYQATYDSAKGDLAKAQAAANIAELTVKRYQKLLGTQYISKQEYDQALADAQQATAAVVAA
KAAVETARINLAYTKVTSPISGRIGKSSVTEGALVQNGQASALATVQQLDPIYVDVTQSSNDFLRLKQELANGSLKQENG
KAKVDLVTSDGIKFPQSGTLEFSDVTVDQSTGSITLRAIFPNPDHTLLPGMFVRARLQEGTKPTALLVPQQGVTRTPRGD
ATVLVVGADNKVETRQIVASQAIGDKWLVTDGLKAGDRVVVSGLQKVRPGAQVKVQEITADNKQQAASGDQPAQPRS

Sequences:

>Translated_397_residues
MNKNRGLTPLAVVLMLSGSLALTGCDDKQDQQGGQQMPEVGVVTLKTEPLQITTELPGRTVAYRIAEVRPQVSGIILKRN
FVEGSDIEAGVSLYQIDPATYQATYDSAKGDLAKAQAAANIAELTVKRYQKLLGTQYISKQEYDQALADAQQATAAVVAA
KAAVETARINLAYTKVTSPISGRIGKSSVTEGALVQNGQASALATVQQLDPIYVDVTQSSNDFLRLKQELANGSLKQENG
KAKVDLVTSDGIKFPQSGTLEFSDVTVDQSTGSITLRAIFPNPDHTLLPGMFVRARLQEGTKPTALLVPQQGVTRTPRGD
ATVLVVGADNKVETRQIVASQAIGDKWLVTDGLKAGDRVVVSGLQKVRPGAQVKVQEITADNKQQAASGDQPAQPRS
>Mature_397_residues
MNKNRGLTPLAVVLMLSGSLALTGCDDKQDQQGGQQMPEVGVVTLKTEPLQITTELPGRTVAYRIAEVRPQVSGIILKRN
FVEGSDIEAGVSLYQIDPATYQATYDSAKGDLAKAQAAANIAELTVKRYQKLLGTQYISKQEYDQALADAQQATAAVVAA
KAAVETARINLAYTKVTSPISGRIGKSSVTEGALVQNGQASALATVQQLDPIYVDVTQSSNDFLRLKQELANGSLKQENG
KAKVDLVTSDGIKFPQSGTLEFSDVTVDQSTGSITLRAIFPNPDHTLLPGMFVRARLQEGTKPTALLVPQQGVTRTPRGD
ATVLVVGADNKVETRQIVASQAIGDKWLVTDGLKAGDRVVVSGLQKVRPGAQVKVQEITADNKQQAASGDQPAQPRS

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=397, Percent_Identity=91.4357682619647, Blast_Score=694, Evalue=0.0,
Organism=Escherichia coli, GI1789665, Length=374, Percent_Identity=68.716577540107, Blast_Score=508, Evalue=1e-145,
Organism=Escherichia coli, GI1789929, Length=391, Percent_Identity=53.4526854219949, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI87082036, Length=321, Percent_Identity=30.2180685358255, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42221; Mature: 42221

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNRGLTPLAVVLMLSGSLALTGCDDKQDQQGGQQMPEVGVVTLKTEPLQITTELPGRT
CCCCCCCCHHHHHHHHCCCEEEECCCCCHHHHCCCCCCCCCEEEEECCCEEEEECCCCCE
VAYRIAEVRPQVSGIILKRNFVEGSDIEAGVSLYQIDPATYQATYDSAKGDLAKAQAAAN
EEEEHHHHCCCHHEEEEEECCCCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHHH
IAELTVKRYQKLLGTQYISKQEYDQALADAQQATAAVVAAKAAVETARINLAYTKVTSPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEECCCC
SGRIGKSSVTEGALVQNGQASALATVQQLDPIYVDVTQSSNDFLRLKQELANGSLKQENG
CCCCCCCCCCCCCEECCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCC
KAKVDLVTSDGIKFPQSGTLEFSDVTVDQSTGSITLRAIFPNPDHTLLPGMFVRARLQEG
CEEEEEEECCCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHCC
TKPTALLVPQQGVTRTPRGDATVLVVGADNKVETRQIVASQAIGDKWLVTDGLKAGDRVV
CCCEEEEECCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHH
VSGLQKVRPGAQVKVQEITADNKQQAASGDQPAQPRS
HHHHHHHCCCCCEEEEEECCCCHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNKNRGLTPLAVVLMLSGSLALTGCDDKQDQQGGQQMPEVGVVTLKTEPLQITTELPGRT
CCCCCCCCHHHHHHHHCCCEEEECCCCCHHHHCCCCCCCCCEEEEECCCEEEEECCCCCE
VAYRIAEVRPQVSGIILKRNFVEGSDIEAGVSLYQIDPATYQATYDSAKGDLAKAQAAAN
EEEEHHHHCCCHHEEEEEECCCCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHHHHHHH
IAELTVKRYQKLLGTQYISKQEYDQALADAQQATAAVVAAKAAVETARINLAYTKVTSPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEECCCC
SGRIGKSSVTEGALVQNGQASALATVQQLDPIYVDVTQSSNDFLRLKQELANGSLKQENG
CCCCCCCCCCCCCEECCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCC
KAKVDLVTSDGIKFPQSGTLEFSDVTVDQSTGSITLRAIFPNPDHTLLPGMFVRARLQEG
CEEEEEEECCCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHCC
TKPTALLVPQQGVTRTPRGDATVLVVGADNKVETRQIVASQAIGDKWLVTDGLKAGDRVV
CCCEEEEECCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHH
VSGLQKVRPGAQVKVQEITADNKQQAASGDQPAQPRS
HHHHHHHCCCCCEEEEEECCCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]