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The map label for this gene is aes [H]

Identifier: 29142759

GI number: 29142759

Start: 2439231

End: 2440202

Strand: Direct

Name: aes [H]

Synonym: t2370

Alternate gene names: 29142759

Gene position: 2439231-2440202 (Clockwise)

Preceding gene: 29142757

Following gene: 29142768

Centisome position: 50.9

GC content: 56.17

Gene sequence:

>972_bases
ATGAAGCCGGAAAATAAAATTCCCGTACTCACCCGACTGTCTGACGAAATGACAGCCGTCGTAAACTTCCAGCAACCAGG
CCTTCCCCCCTGGCCTGCCGATGGCGATATTGAGACCCAGCGTCAGTATTATCTTCTTGAGCGCCGTTTCTGGAATGCCG
ACGCGCCGTCCATGACCACCCGCACCTGCGCCGTCTCTACGCCTTATGGCGATGTAACAACCCGGCTCTACTCGCCGCAG
CCGACCAGTCAGGCCACCTTATACTATCTGCACGGCGGCGGATTTATCCTCGGTAATCTGGATACGCATGACCGGATAAT
GCGTCTGCTGGCGCGTTATACCGGCTGCACCGTGATCGGTATTGATTATTCGCTGTCGCCGCAAGCGCGTTATCCGCAGG
CCATTGAAGAAACCGTCGCGGTATGCAGCTACTTCTCTCAACATGCCGATGAATACTCGCTCAACGTGGAGGAAATCGGT
TTCGCCGGAGATTCCGCAGGCGCCATGCTGGCGCTTGCCAGCGCGCTCTGGCTACGTGATAAGCACATTCGCTGCGGTAA
CGTTATCGCGATTCTGTTGTGGTATGGGCTGTATGGGTTGCAAGATTCGGTAAGCCGCCGTCTGTTCGGCGGCGCGTGGG
ACGGCCTGACGCGTGAAGATCTGGACATGTACGAAAAGGCCTATCTGCGTAACGATGAAGATCGGGAATCGCCCTGGTAT
TGTCTGTTTAATAACGATCTCACGCGCGATGTGCCGCCCTGCTTTATTGCCAGCGCGGAGTTTGATCCGCTCATTGATGA
CAGCCGCTTGCTGCACCAGACATTACAGGCGCACCAGCAGCCCTGCGAATACAAAATGTATCCCGGCACGCTGCACGCCT
TCCTGCACTATTCGCGAATGATGACAATCGCCGACGATGCCTTGCAGGACGGCGCACGCTTCTTTATGGCGAGGATGAAG
ACGCCGCGTTAA

Upstream 100 bases:

>100_bases
GCTGCGCGCGACGGACGATGGCGTTTCAATATCGGACGACGAGATCGCAGTTTCTCCAGCCGCGTTTTTTACAATGGTTC
ACTTTCACAAGGAGCGTTTT

Downstream 100 bases:

>100_bases
CGGTACGGCGCCGTGAGTTTCAGTATCATATCAATATGTTCCGGCGTCGCGTTCAGCGCCTGAATGTACGCATATTTTTT
ACCGCCCGCTTCAAGGAAAA

Product: acetyl esterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKPENKIPVLTRLSDEMTAVVNFQQPGLPPWPADGDIETQRQYYLLERRFWNADAPSMTTRTCAVSTPYGDVTTRLYSPQ
PTSQATLYYLHGGGFILGNLDTHDRIMRLLARYTGCTVIGIDYSLSPQARYPQAIEETVAVCSYFSQHADEYSLNVEEIG
FAGDSAGAMLALASALWLRDKHIRCGNVIAILLWYGLYGLQDSVSRRLFGGAWDGLTREDLDMYEKAYLRNDEDRESPWY
CLFNNDLTRDVPPCFIASAEFDPLIDDSRLLHQTLQAHQQPCEYKMYPGTLHAFLHYSRMMTIADDALQDGARFFMARMK
TPR

Sequences:

>Translated_323_residues
MKPENKIPVLTRLSDEMTAVVNFQQPGLPPWPADGDIETQRQYYLLERRFWNADAPSMTTRTCAVSTPYGDVTTRLYSPQ
PTSQATLYYLHGGGFILGNLDTHDRIMRLLARYTGCTVIGIDYSLSPQARYPQAIEETVAVCSYFSQHADEYSLNVEEIG
FAGDSAGAMLALASALWLRDKHIRCGNVIAILLWYGLYGLQDSVSRRLFGGAWDGLTREDLDMYEKAYLRNDEDRESPWY
CLFNNDLTRDVPPCFIASAEFDPLIDDSRLLHQTLQAHQQPCEYKMYPGTLHAFLHYSRMMTIADDALQDGARFFMARMK
TPR
>Mature_323_residues
MKPENKIPVLTRLSDEMTAVVNFQQPGLPPWPADGDIETQRQYYLLERRFWNADAPSMTTRTCAVSTPYGDVTTRLYSPQ
PTSQATLYYLHGGGFILGNLDTHDRIMRLLARYTGCTVIGIDYSLSPQARYPQAIEETVAVCSYFSQHADEYSLNVEEIG
FAGDSAGAMLALASALWLRDKHIRCGNVIAILLWYGLYGLQDSVSRRLFGGAWDGLTREDLDMYEKAYLRNDEDRESPWY
CLFNNDLTRDVPPCFIASAEFDPLIDDSRLLHQTLQAHQQPCEYKMYPGTLHAFLHYSRMMTIADDALQDGARFFMARMK
TPR

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=141, Percent_Identity=32.6241134751773, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI68299767, Length=132, Percent_Identity=30.3030303030303, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI21328446, Length=125, Percent_Identity=32, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1786682, Length=319, Percent_Identity=74.9216300940439, Blast_Score=511, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI72001146, Length=158, Percent_Identity=28.4810126582279, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17540028, Length=182, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24656084, Length=108, Percent_Identity=31.4814814814815, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24656076, Length=108, Percent_Identity=31.4814814814815, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI20130169, Length=108, Percent_Identity=31.4814814814815, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.-

Molecular weight: Translated: 36790; Mature: 36790

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPENKIPVLTRLSDEMTAVVNFQQPGLPPWPADGDIETQRQYYLLERRFWNADAPSMTT
CCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCE
RTCAVSTPYGDVTTRLYSPQPTSQATLYYLHGGGFILGNLDTHDRIMRLLARYTGCTVIG
EEEEEECCCCCHHEEEECCCCCCCEEEEEEECCEEEEECCCHHHHHHHHHHHHCCCEEEE
IDYSLSPQARYPQAIEETVAVCSYFSQHADEYSLNVEEIGFAGDSAGAMLALASALWLRD
ECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHC
KHIRCGNVIAILLWYGLYGLQDSVSRRLFGGAWDGLTREDLDMYEKAYLRNDEDRESPWY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEE
CLFNNDLTRDVPPCFIASAEFDPLIDDSRLLHQTLQAHQQPCEYKMYPGTLHAFLHYSRM
EEECCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHH
MTIADDALQDGARFFMARMKTPR
HHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKPENKIPVLTRLSDEMTAVVNFQQPGLPPWPADGDIETQRQYYLLERRFWNADAPSMTT
CCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCE
RTCAVSTPYGDVTTRLYSPQPTSQATLYYLHGGGFILGNLDTHDRIMRLLARYTGCTVIG
EEEEEECCCCCHHEEEECCCCCCCEEEEEEECCEEEEECCCHHHHHHHHHHHHCCCEEEE
IDYSLSPQARYPQAIEETVAVCSYFSQHADEYSLNVEEIGFAGDSAGAMLALASALWLRD
ECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHC
KHIRCGNVIAILLWYGLYGLQDSVSRRLFGGAWDGLTREDLDMYEKAYLRNDEDRESPWY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEE
CLFNNDLTRDVPPCFIASAEFDPLIDDSRLLHQTLQAHQQPCEYKMYPGTLHAFLHYSRM
EEECCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHH
MTIADDALQDGARFFMARMKTPR
HHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA