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The map label for this gene is gcl [H]

Identifier: 29142734

GI number: 29142734

Start: 2408630

End: 2410411

Strand: Reverse

Name: gcl [H]

Synonym: t2343

Alternate gene names: 29142734

Gene position: 2410411-2408630 (Counterclockwise)

Preceding gene: 29142735

Following gene: 29142733

Centisome position: 50.3

GC content: 54.1

Gene sequence:

>1782_bases
ATGGCAAAAATGAGAGCCGTGGATGCGGCAATGTATGTGCTGGAAAAAGAAGGTATCACCACAGCATTCGGCGTTCCTGG
GGCTGCAATCAACCCCTTCTATTCTGCAATGCGTAAGCATGGAGGCATTCGCCATATACTGGCGCGTCATGTGGAAGGCG
CTTCGCATATGGCTGAGGGCTACACACGTGCGGCGGCAGGTAATATTGGGGTCTGCCTGGGCACTTCCGGCCCGGCGGGA
ACCGACATGATCACCGCGCTCTATTCCGCTTCTGCCGACTCTATCCCGATTTTATGCATTACCGGCCAGGCGCCGCGAGC
GCGTCTGCACAAGGAAGATTTCCAGGCAGTCGATATTGAAGCGATTGCAAAACCAGTCAGCAAGATGGCAGTGACGGTTC
GTGAGGCGGCGCTGGTGCCGCGCGTATTACAGCAGGCTTTTCATCTGATGCGCTCAGGCCGCCCAGGTCCTGTGCTGGTT
GATTTACCGTTTGATGTCCAGGTTGCGGAAATCGAATTCGATCCGGATATGTATGAGCCGCTGCCGGTATATAAGCCAGC
CGCCAGCCGAGTACAGATTGAAAAGGCGCTGGAGATGCTGATTCAGTCCGAGCGTCCGGTAATTGTCGCGGGCGGCGGGG
TTATTAACGCCGACGCCGCGCCACTGCTGCAGCAGTTTGCCGAACTGATCAACGTACCGGTGATCCCTACCCTGATGGGC
TGGGGCTGTACCCTGGACGATCACCCCTTAATGGCGGGGATGGTCGGCCTGCAAACGGCGCACCGCTACGGCAACGCCAC
TCTGCTGGCATCCGATATGGTTTTTGGCATCGGTAACCGTTTCGCCAACCGTCATACGGGGTCCGTTGAGAAATATACTC
AGGGTCGAAAAATCATCCATATCGATATTGAACCGACGCAAATTGGCCGCGTGTTGTGCCCGGATCTGGGCATCGTTTCT
GACGCCAAAGCGGCGCTAACGTTGCTGATCGATGTCGCGCAGGAGATGCAAAAAGCGGGGCGTTTGCCATGCCGGAAGAC
CTGGGTGGACGAATGCCAGCAGCGTAAACGGACTTTGCTGCGTAAAACCCACTTTGACAACGTGCCAGTGAAGCCGCAGC
GCGTCTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTGTGCTATGTCACCACCATTGGATTGTCGCAAATCGCGGCA
GCGCAGATGCTGCATGTGTTTAAAGACAGACACTGGATCAACTGCGGCCAGGCGGGGCCTCTTGGCTGGACAATCCCGGC
GGCATTAGGGGTTTGTGCTGCCGATCCACAACGCAATGTGGTGGCGATTTCCGGCGATTTCGACTTCCAGTTCCTGATTG
AAGAGTTAGCCGTCGGCGCGCAGTTTAACATTCCGTATATCCATGTGCTGGTTAACAACGCTTATCTTGGTCTGATTCGC
CAGTCGCAACGCGCATTTGACATGGACTATTGCGTGCAGCTCGCCTTTGAAAATATCAACTCCAGCGAAGTGAATGGCTA
TGGCGTTGACCATGTAAAAGTGGCAGAAGGGTTAGGCTGTAAAGCAATTCGTGTCTTTAAACCGGAAGATATTGCGCCTG
CATTTGAACAAGCTAAAGCGCTGATGGCGCAATATCGCGTTCCGGTGGTGGTGGAAGTCATTCTGGAACGCGTAACCAAT
ATTTCCATGGGCGACGAACTGGATAACGTAACCGAGTTTGAAGAGGTTGCGGATAGTGCGAAAGATGCGCCAACAGAAAC
CTGTTTTATGAAATATGAGTAA

Upstream 100 bases:

>100_bases
ACCCCCTGTAGCCTGAGCCGCATCCCGCTCGCTGGAGCGGGACTTTTTTAAAATTTTTAAAAGTTGGAAACTTTTTCCAA
TAAAAAGAGGTAGCAATAAA

Downstream 100 bases:

>100_bases
GGGAGGTAATCGATGTTACGTTTCTCTGCCAATCTTTCTATGCTGTTTACGGAATATGATTTCCTGGAACGCTTTGATAA
AGCGGCGCAGTCGGGATTTC

Product: glyoxylate carboligase

Products: NA

Alternate protein names: Tartronate-semialdehyde synthase [H]

Number of amino acids: Translated: 593; Mature: 592

Protein sequence:

>593_residues
MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEGYTRAAAGNIGVCLGTSGPAG
TDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
DLPFDVQVAEIEFDPDMYEPLPVYKPAASRVQIEKALEMLIQSERPVIVAGGGVINADAAPLLQQFAELINVPVIPTLMG
WGCTLDDHPLMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTQGRKIIHIDIEPTQIGRVLCPDLGIVS
DAKAALTLLIDVAQEMQKAGRLPCRKTWVDECQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAA
AQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPQRNVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAYLGLIR
QSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTN
ISMGDELDNVTEFEEVADSAKDAPTETCFMKYE

Sequences:

>Translated_593_residues
MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEGYTRAAAGNIGVCLGTSGPAG
TDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
DLPFDVQVAEIEFDPDMYEPLPVYKPAASRVQIEKALEMLIQSERPVIVAGGGVINADAAPLLQQFAELINVPVIPTLMG
WGCTLDDHPLMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTQGRKIIHIDIEPTQIGRVLCPDLGIVS
DAKAALTLLIDVAQEMQKAGRLPCRKTWVDECQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAA
AQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPQRNVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAYLGLIR
QSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTN
ISMGDELDNVTEFEEVADSAKDAPTETCFMKYE
>Mature_592_residues
AKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEGYTRAAAGNIGVCLGTSGPAGT
DMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLVD
LPFDVQVAEIEFDPDMYEPLPVYKPAASRVQIEKALEMLIQSERPVIVAGGGVINADAAPLLQQFAELINVPVIPTLMGW
GCTLDDHPLMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTQGRKIIHIDIEPTQIGRVLCPDLGIVSD
AKAALTLLIDVAQEMQKAGRLPCRKTWVDECQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAAA
QMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPQRNVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAYLGLIRQ
SQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNI
SMGDELDNVTEFEEVADSAKDAPTETCFMKYE

Specific function: Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) [H]

COG id: COG3960

COG function: function code R; Glyoxylate carboligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=572, Percent_Identity=22.9020979020979, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI21361361, Length=558, Percent_Identity=24.0143369175627, Blast_Score=105, Evalue=9e-23,
Organism=Escherichia coli, GI1786717, Length=593, Percent_Identity=95.7841483979764, Blast_Score=1181, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=544, Percent_Identity=34.1911764705882, Blast_Score=308, Evalue=5e-85,
Organism=Escherichia coli, GI1790104, Length=476, Percent_Identity=33.8235294117647, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1787096, Length=548, Percent_Identity=26.2773722627737, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788716, Length=486, Percent_Identity=26.7489711934156, Blast_Score=135, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17531299, Length=503, Percent_Identity=25.844930417495, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17531301, Length=503, Percent_Identity=25.844930417495, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17542570, Length=543, Percent_Identity=24.1252302025783, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323755, Length=594, Percent_Identity=30.4713804713805, Blast_Score=258, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6320816, Length=563, Percent_Identity=23.4458259325044, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321524, Length=497, Percent_Identity=21.9315895372233, Blast_Score=77, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6323163, Length=501, Percent_Identity=21.3572854291417, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922626, Length=489, Percent_Identity=24.3353783231084, Blast_Score=133, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006397
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.47 [H]

Molecular weight: Translated: 64800; Mature: 64668

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEG
CCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHH
YTRAAAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIE
HHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHCHHCCCEECHH
AIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLVDLPFDVQVAEIEFDPDMYEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECCCCCCC
LPVYKPAASRVQIEKALEMLIQSERPVIVAGGGVINADAAPLLQQFAELINVPVIPTLMG
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCEECCCHHHHHHHHHHHHCCCCHHHHHC
WGCTLDDHPLMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTQGRKIIH
CCCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHCCCCCCCHHHHHCCCEEEE
IDIEPTQIGRVLCPDLGIVSDAKAALTLLIDVAQEMQKAGRLPCRKTWVDECQQRKRTLL
EECCHHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAAAQMLHVFKDRHWINCGQAGP
HHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCEECCCCCCC
LGWTIPAALGVCAADPQRNVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAYLGLIR
CCEEHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHH
QSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKA
HHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEHHCCCCCCEEEEECCCCCCHHHHHHHH
LMAQYRVPVVVEVILERVTNISMGDELDNVTEFEEVADSAKDAPTETCFMKYE
HHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECC
>Mature Secondary Structure 
AKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEG
CCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHH
YTRAAAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIE
HHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHCHHCCCEECHH
AIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLVDLPFDVQVAEIEFDPDMYEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEECCCCCCC
LPVYKPAASRVQIEKALEMLIQSERPVIVAGGGVINADAAPLLQQFAELINVPVIPTLMG
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCEECCCHHHHHHHHHHHHCCCCHHHHHC
WGCTLDDHPLMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTQGRKIIH
CCCCCCCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHCCCCCCCHHHHHCCCEEEE
IDIEPTQIGRVLCPDLGIVSDAKAALTLLIDVAQEMQKAGRLPCRKTWVDECQQRKRTLL
EECCHHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAAAQMLHVFKDRHWINCGQAGP
HHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCEECCCCCCC
LGWTIPAALGVCAADPQRNVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAYLGLIR
CCEEHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHH
QSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKA
HHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEHHCCCCCCEEEEECCCCCCHHHHHHHH
LMAQYRVPVVVEVILERVTNISMGDELDNVTEFEEVADSAKDAPTETCFMKYE
HHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]