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The map label for this gene is hyi [H]

Identifier: 29142733

GI number: 29142733

Start: 2407841

End: 2408617

Strand: Reverse

Name: hyi [H]

Synonym: t2342

Alternate gene names: 29142733

Gene position: 2408617-2407841 (Counterclockwise)

Preceding gene: 29142734

Following gene: 29142732

Centisome position: 50.26

GC content: 45.82

Gene sequence:

>777_bases
ATGTTACGTTTCTCTGCCAATCTTTCTATGCTGTTTACGGAATATGATTTCCTGGAACGCTTTGATAAAGCGGCGCAGTC
GGGATTTCGCGGCGTGGAATTCATGTTTCCCTACGATGACGACATCGAAGTGTTAAAACGTAAGCTCAGGGATAATAATC
TGGAACATACGTTACATAATTTACCGGCTGGCGACTGGGCGGCTGGCGAACGTGGAATTGCCTGTATTCCTGGGCGCGAA
GAAGAGTTTCGCGATGGCGTAGCGGCCGCAATCCGCTATGCACGCGCGTTAGGCAATAAGAAAATAAATTGTCTGGTGGG
TAAAACGCCGTCAGGATTCTCCGCAACGGAGATCTACGACACGTTGGTCGAAAACCTGCGCTATGCGGCAAATATGCTGG
CGAAAGAAGACATTTTATTACTTATCGAACCTATTAATCACTTTGATATGCCGGGATTCCATCTCACCGGCACTCAACAG
GCGTTAGTATTAATTAAGGATATCGGCAGCGATAATATCAAAATTCAGTATGATATTTATCACATGCAACGCATGGAAGG
GGAATTAACGCAAACCATGACGGCATGGGCCGATAAAATAGGCCATTTACAGATTGCTGATAACCCGCGTCGCGGCGAGC
CGGGGACCGGGGAAATCAATTATGACTTTATTTTTAATGTCATTAAACAGTCTGATTATGATGGCTGGGTTGGATGTGAA
TATAAACCACTGACTACCGCGGAAGCGGGATTATCGTGGATTAATCAGTACCGTTAA

Upstream 100 bases:

>100_bases
CGAACTGGATAACGTAACCGAGTTTGAAGAGGTTGCGGATAGTGCGAAAGATGCGCCAACAGAAACCTGTTTTATGAAAT
ATGAGTAAGGGAGGTAATCG

Downstream 100 bases:

>100_bases
AACGCAGAATAATTCGGGCAGCGCTTTTTTTTCCACTGTGTTGGAAGGGGATTGTGTTGCCTGAATCTGGAAACACCGAA
AGCAATAAAAGAGGTTGTAT

Product: hydroxypyruvate isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLRFSANLSMLFTEYDFLERFDKAAQSGFRGVEFMFPYDDDIEVLKRKLRDNNLEHTLHNLPAGDWAAGERGIACIPGRE
EEFRDGVAAAIRYARALGNKKINCLVGKTPSGFSATEIYDTLVENLRYAANMLAKEDILLLIEPINHFDMPGFHLTGTQQ
ALVLIKDIGSDNIKIQYDIYHMQRMEGELTQTMTAWADKIGHLQIADNPRRGEPGTGEINYDFIFNVIKQSDYDGWVGCE
YKPLTTAEAGLSWINQYR

Sequences:

>Translated_258_residues
MLRFSANLSMLFTEYDFLERFDKAAQSGFRGVEFMFPYDDDIEVLKRKLRDNNLEHTLHNLPAGDWAAGERGIACIPGRE
EEFRDGVAAAIRYARALGNKKINCLVGKTPSGFSATEIYDTLVENLRYAANMLAKEDILLLIEPINHFDMPGFHLTGTQQ
ALVLIKDIGSDNIKIQYDIYHMQRMEGELTQTMTAWADKIGHLQIADNPRRGEPGTGEINYDFIFNVIKQSDYDGWVGCE
YKPLTTAEAGLSWINQYR
>Mature_258_residues
MLRFSANLSMLFTEYDFLERFDKAAQSGFRGVEFMFPYDDDIEVLKRKLRDNNLEHTLHNLPAGDWAAGERGIACIPGRE
EEFRDGVAAAIRYARALGNKKINCLVGKTPSGFSATEIYDTLVENLRYAANMLAKEDILLLIEPINHFDMPGFHLTGTQQ
ALVLIKDIGSDNIKIQYDIYHMQRMEGELTQTMTAWADKIGHLQIADNPRRGEPGTGEINYDFIFNVIKQSDYDGWVGCE
YKPLTTAEAGLSWINQYR

Specific function: Catalyzes the reversible isomerization between hydroxypyruvate and 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) [H]

COG id: COG3622

COG function: function code G; Hydroxypyruvate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hyi family [H]

Homologues:

Organism=Homo sapiens, GI300068962, Length=263, Percent_Identity=41.4448669201521, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI300068964, Length=244, Percent_Identity=40.9836065573771, Blast_Score=181, Evalue=5e-46,
Organism=Escherichia coli, GI1786718, Length=258, Percent_Identity=84.1085271317829, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI1789095, Length=258, Percent_Identity=39.1472868217054, Blast_Score=211, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI17552022, Length=253, Percent_Identity=37.9446640316205, Blast_Score=183, Evalue=6e-47,
Organism=Drosophila melanogaster, GI17530883, Length=259, Percent_Identity=35.5212355212355, Blast_Score=171, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017643
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =5.3.1.22 [H]

Molecular weight: Translated: 29344; Mature: 29344

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRFSANLSMLFTEYDFLERFDKAAQSGFRGVEFMFPYDDDIEVLKRKLRDNNLEHTLHN
CEEECCCCEEEHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCHHHHHHH
LPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKINCLVGKTPSGFSATEIYD
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHH
TLVENLRYAANMLAKEDILLLIEPINHFDMPGFHLTGTQQALVLIKDIGSDNIKIQYDIY
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEECCCHHEEEEEEECCCCCEEEEEEHH
HMQRMEGELTQTMTAWADKIGHLQIADNPRRGEPGTGEINYDFIFNVIKQSDYDGWVGCE
HHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCEEHHHHHHHHHCCCCCCCCCCC
YKPLTTAEAGLSWINQYR
CCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MLRFSANLSMLFTEYDFLERFDKAAQSGFRGVEFMFPYDDDIEVLKRKLRDNNLEHTLHN
CEEECCCCEEEHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCHHHHHHH
LPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKINCLVGKTPSGFSATEIYD
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHH
TLVENLRYAANMLAKEDILLLIEPINHFDMPGFHLTGTQQALVLIKDIGSDNIKIQYDIY
HHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEECCCHHEEEEEEECCCCCEEEEEEHH
HMQRMEGELTQTMTAWADKIGHLQIADNPRRGEPGTGEINYDFIFNVIKQSDYDGWVGCE
HHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCEEHHHHHHHHHCCCCCCCCCCC
YKPLTTAEAGLSWINQYR
CCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8440684; 10561547; 9278503 [H]