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The map label for this gene is citF [H]

Identifier: 29142646

GI number: 29142646

Start: 2310948

End: 2312477

Strand: Direct

Name: citF [H]

Synonym: t2246

Alternate gene names: 29142646

Gene position: 2310948-2312477 (Clockwise)

Preceding gene: 29142645

Following gene: 29142647

Centisome position: 48.23

GC content: 58.63

Gene sequence:

>1530_bases
ATGACGCAGAAAAATGAATCTCAACGCCAGGATCGCGTAGTAGCCTGGAGCCGTCACGCTGAAAGCGAACTGAGCGCTTA
CCAAAGTGCGGCAAAATTGGATCTACAAGCGCAAAAACCGCGCGACCACAAACTGTGCGCCAGCCTGGAAGAGGCCATCC
GCCGTTCCGGTTTACGCGACGGCATGACGCTTTCCTTTCACCACGCTTTTCGCGGCGGCGATCTGACGATTAACCTGGTG
ATGGAAACCATCGCGAAGATGGGCTTCAAAAATCTGACCCTGGCCTCCAGCTCGCTGAGCGACTGCCACGCGCCGCTGGT
TGAACACATTCGTAACGGGGTGGTCAGCCGCATCTACACGTCAGGCCTGCGCGGCCCGCTGGCGGAAGAAATTTCTCGCG
GCCTGCTGGCTGAGCCGGTTCAGATTCACTCCCACGGCGGTCGTGTGCATCTGGTACAAAGCGGCGAACTCAATATTGAT
GTTGCGTTTCTTGGCGTGCCCTCCTGCGATGAATTCGGTAATGCTAACGGTTATACCGGCAAAGCCTGTTGCGGTTCTCT
GGGCTACGCGATGGTGGATGCCGACAGCGCGAAGCAGGTCGTGATGCTGACGGAGCAACTGCTGCCGTATCCGCATAACC
CGGCAAGCATCGCTCAGGATCAGGTTGATCTGATCGTGCAAATCGAAGAAGTCGGTGATGCAAACAAAATCGGCGCTGGC
GCGACCCGCATGACCACTAATCCGCGTGAACTGCTGATCGCCCGCAGCGCGGCGGAAGTCATTGCTCACTCCGGCTACTT
CAACGAAGGTTTCTCCCTGCAAACCGGCACCGGCGGCGCATCGCTGGCGGTCACCCGCTTCCTGGAAGACAAAATGCGTA
GCCGCAACATTGTGGCTAATTTCGCGCTGGGCGGTATCACCGCCACCATGGTCAACCTGCATGAAAAGGGCTTAATCCGC
AAACTGCTTGACGTGCAGAGCTTCGATCGTAACGCTGCGCAATCTTTGGCCCGCAACCCGAACCATATCGAAATCAGCGC
TAACCAGTACGCCAACTGGGGCTCTAAAGGCGCTTCCGTCGACCGTCTGGACGTGGTGGTACTGAGCGCGCTGGAAGTAG
ACACCCACTTCAATGTTAACGTGCTGACCGGCTCCGACGGCGTTCTACGCGGCGCGTCCGGCGGTCACTGCGATACCGCC
GTCGCGGCGGCGCTGTCCATCATCGTCGCGCCGCTGGTGCGCGGTCGCATCCCAACGCTGGTTGATAACGTCACCACCTG
CGTCACACCGGGCTCCAGCGTTGACATTCTGGTCACCGATCACGGTATCGCCGTCAATCCGGCGCGTCCGGAGTTGGCTG
AACGCCTGAAAGCAGCGGGAATGAAAGTGGTATCGATTGAGTGGCTGCGCGAACGCGCGCAACTGCTTACCGGTCAGCCC
CGCCCTATCGAATTCACCGACCGCGTGATTGCTGTCGTGCGTTATCGCGACGGTTCAGTGATCGATGTGGTGCATCAGGT
GAAGGAATAA

Upstream 100 bases:

>100_bases
CCTTAACGGCAAGATGGTCGACAGCCCGGTGATTGAACGCGCCCGTCTGGTGCTCTCCCGTGCAGAACTTTCCGGTATTC
GCGAAGAATAAGGCAAAAAA

Downstream 100 bases:

>100_bases
GCCACGCCACTCCACTGGTCTCCTTTACCGTGGTGGCGCCAGGCCCGATGAAAGACAGCGCGTTAACCCGCCGGATCTTT
AATCACGGCGTGACTGCCCT

Product: citrate lyase subunit alpha

Products: NA

Alternate protein names: Citrase alpha chain; Citrate (pro-3S)-lyase alpha chain; Citrate CoA-transferase subunit [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MTQKNESQRQDRVVAWSRHAESELSAYQSAAKLDLQAQKPRDHKLCASLEEAIRRSGLRDGMTLSFHHAFRGGDLTINLV
METIAKMGFKNLTLASSSLSDCHAPLVEHIRNGVVSRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNID
VAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADSAKQVVMLTEQLLPYPHNPASIAQDQVDLIVQIEEVGDANKIGAG
ATRMTTNPRELLIARSAAEVIAHSGYFNEGFSLQTGTGGASLAVTRFLEDKMRSRNIVANFALGGITATMVNLHEKGLIR
KLLDVQSFDRNAAQSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEVDTHFNVNVLTGSDGVLRGASGGHCDTA
VAAALSIIVAPLVRGRIPTLVDNVTTCVTPGSSVDILVTDHGIAVNPARPELAERLKAAGMKVVSIEWLRERAQLLTGQP
RPIEFTDRVIAVVRYRDGSVIDVVHQVKE

Sequences:

>Translated_509_residues
MTQKNESQRQDRVVAWSRHAESELSAYQSAAKLDLQAQKPRDHKLCASLEEAIRRSGLRDGMTLSFHHAFRGGDLTINLV
METIAKMGFKNLTLASSSLSDCHAPLVEHIRNGVVSRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNID
VAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADSAKQVVMLTEQLLPYPHNPASIAQDQVDLIVQIEEVGDANKIGAG
ATRMTTNPRELLIARSAAEVIAHSGYFNEGFSLQTGTGGASLAVTRFLEDKMRSRNIVANFALGGITATMVNLHEKGLIR
KLLDVQSFDRNAAQSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEVDTHFNVNVLTGSDGVLRGASGGHCDTA
VAAALSIIVAPLVRGRIPTLVDNVTTCVTPGSSVDILVTDHGIAVNPARPELAERLKAAGMKVVSIEWLRERAQLLTGQP
RPIEFTDRVIAVVRYRDGSVIDVVHQVKE
>Mature_508_residues
TQKNESQRQDRVVAWSRHAESELSAYQSAAKLDLQAQKPRDHKLCASLEEAIRRSGLRDGMTLSFHHAFRGGDLTINLVM
ETIAKMGFKNLTLASSSLSDCHAPLVEHIRNGVVSRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNIDV
AFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADSAKQVVMLTEQLLPYPHNPASIAQDQVDLIVQIEEVGDANKIGAGA
TRMTTNPRELLIARSAAEVIAHSGYFNEGFSLQTGTGGASLAVTRFLEDKMRSRNIVANFALGGITATMVNLHEKGLIRK
LLDVQSFDRNAAQSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEVDTHFNVNVLTGSDGVLRGASGGHCDTAV
AAALSIIVAPLVRGRIPTLVDNVTTCVTPGSSVDILVTDHGIAVNPARPELAERLKAAGMKVVSIEWLRERAQLLTGQPR
PIEFTDRVIAVVRYRDGSVIDVVHQVKE

Specific function: Represents a citrate:acetyl-ACP transferase [H]

COG id: COG3051

COG function: function code C; Citrate lyase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786832, Length=510, Percent_Identity=88.0392156862745, Blast_Score=933, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006472 [H]

Pfam domain/function: PF04223 CitF [H]

EC number: =4.1.3.6; =2.8.3.10 [H]

Molecular weight: Translated: 54780; Mature: 54649

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKNESQRQDRVVAWSRHAESELSAYQSAAKLDLQAQKPRDHKLCASLEEAIRRSGLRD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCC
GMTLSFHHAFRGGDLTINLVMETIAKMGFKNLTLASSSLSDCHAPLVEHIRNGVVSRIYT
CCEEEEEEEECCCCEEHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
SGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNIDVAFLGVPSCDEFGNANGYTG
HCCCCCHHHHHHCCHHCCCCEEECCCCEEEEEECCCEEEEEEEEECCCCHHCCCCCCCCC
KACCGSLGYAMVDADSAKQVVMLTEQLLPYPHNPASIAQDQVDLIVQIEEVGDANKIGAG
HHHHHHCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHCCCEEEEEEECCCCCCCCCCC
ATRMTTNPRELLIARSAAEVIAHSGYFNEGFSLQTGTGGASLAVTRFLEDKMRSRNIVAN
CCCCCCCHHHHEEEHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCEEEE
FALGGITATMVNLHEKGLIRKLLDVQSFDRNAAQSLARNPNHIEISANQYANWGSKGASV
EHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCC
DRLDVVVLSALEVDTHFNVNVLTGSDGVLRGASGGHCDTAVAAALSIIVAPLVRGRIPTL
CHHHHEEHHHEEECCCEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHH
VDNVTTCVTPGSSVDILVTDHGIAVNPARPELAERLKAAGMKVVSIEWLRERAQLLTGQP
HHCCHHEECCCCCEEEEEECCCEEECCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCC
RPIEFTDRVIAVVRYRDGSVIDVVHQVKE
CCCCHHCEEEEEEEECCCCHHHHHHHHCC
>Mature Secondary Structure 
TQKNESQRQDRVVAWSRHAESELSAYQSAAKLDLQAQKPRDHKLCASLEEAIRRSGLRD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCC
GMTLSFHHAFRGGDLTINLVMETIAKMGFKNLTLASSSLSDCHAPLVEHIRNGVVSRIYT
CCEEEEEEEECCCCEEHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
SGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNIDVAFLGVPSCDEFGNANGYTG
HCCCCCHHHHHHCCHHCCCCEEECCCCEEEEEECCCEEEEEEEEECCCCHHCCCCCCCCC
KACCGSLGYAMVDADSAKQVVMLTEQLLPYPHNPASIAQDQVDLIVQIEEVGDANKIGAG
HHHHHHCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHCCCEEEEEEECCCCCCCCCCC
ATRMTTNPRELLIARSAAEVIAHSGYFNEGFSLQTGTGGASLAVTRFLEDKMRSRNIVAN
CCCCCCCHHHHEEEHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCEEEE
FALGGITATMVNLHEKGLIRKLLDVQSFDRNAAQSLARNPNHIEISANQYANWGSKGASV
EHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCC
DRLDVVVLSALEVDTHFNVNVLTGSDGVLRGASGGHCDTAVAAALSIIVAPLVRGRIPTL
CHHHHEEHHHEEECCCEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHH
VDNVTTCVTPGSSVDILVTDHGIAVNPARPELAERLKAAGMKVVSIEWLRERAQLLTGQP
HHCCHHEECCCCCEEEEEECCCEEECCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCC
RPIEFTDRVIAVVRYRDGSVIDVVHQVKE
CCCCHHCEEEEEEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]