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The map label for this gene is citE [H]

Identifier: 29142645

GI number: 29142645

Start: 2310030

End: 2310938

Strand: Direct

Name: citE [H]

Synonym: t2245

Alternate gene names: 29142645

Gene position: 2310030-2310938 (Clockwise)

Preceding gene: 29142644

Following gene: 29142646

Centisome position: 48.21

GC content: 59.63

Gene sequence:

>909_bases
ATGATTTCCGCCTCTCTGCAACAACGTAAAACCCGCACCCGCCGCAGTATGTTATTCGTACCGGGCGCCAACGCGGCGAT
GGTGAACAATTCGTTTATCTACCCGGCCGACGCGCTGATGTTCGACCTGGAAGACTCCGTTGCATTACGCGAAAAAGACG
CGGCGCGCCGTCTGGTATATCACGCGCTGCAACATCCGCTTTACCGTGACGTCGAAACCATCGTCCGCGTGAATGCGCTG
GACTCCGAATGGGGCGTCAACGATCTGGAAGCGGTCGTGCGCGGCGGCGCGGACGTGGTGCGTTTACCGAAAACCGACAC
CGCGCAGGACGTCATTGATATCGAAAACGAAATTCTGCGCATTGAAAACGCCTGCGGTCGCGAACCGGGCAGTACCGGCC
TGTTAGCCGCCGTGGAATCGCCGCTGGGTATTACCCGCGCGGTAGAAATCGCCCACGCCTCTGAACGGTTGATCGGGATT
GCGCTGGGCGCGGAAGACTATGTTCGCAACCTGCGCACCGAACGCTCTCCGGAAGGCACCGAACTGCTGTTCGCCCGCTG
CGCCATTTTGCAGGCCGCGCGTTCCGCCGGCATTCAGGCCTTCGATACCGTCTATTCCGACGCCAACAACGAAGCCGGTT
TCCTGCAAGAAGCCGCCCACATTAAACAGTTGGGCTTTGACGGTAAGTCACTGATCAACCCCCGCCAGATAGAACTGCTG
CACAACCTTTATGCGCCGACGCGTAAAGAGGTCGCCCATGCGCGTCTGGTCGTTGAGGCCGCAGAAGCCGCCGCCCGCGA
AGGTCTCGGCGTCGTTTCCCTTAACGGCAAGATGGTCGACAGCCCGGTGATTGAACGCGCCCGTCTGGTGCTCTCCCGTG
CAGAACTTTCCGGTATTCGCGAAGAATAA

Upstream 100 bases:

>100_bases
TCGATGATAAAGGCGCTCTGGACTGCATTTTGCGCGCCCGACTGGAAACGCTACTGGCGCGCGCCAGCGGTATTGCCGCT
CTGCCCTGGGAGGATCGCCA

Downstream 100 bases:

>100_bases
GGCAAAAAAATGACGCAGAAAAATGAATCTCAACGCCAGGATCGCGTAGTAGCCTGGAGCCGTCACGCTGAAAGCGAACT
GAGCGCTTACCAAAGTGCGG

Product: citrate lyase subunit beta

Products: NA

Alternate protein names: Citrase beta chain; Citrate (pro-3S)-lyase subunit beta; Citryl-CoA lyase subunit [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MISASLQQRKTRTRRSMLFVPGANAAMVNNSFIYPADALMFDLEDSVALREKDAARRLVYHALQHPLYRDVETIVRVNAL
DSEWGVNDLEAVVRGGADVVRLPKTDTAQDVIDIENEILRIENACGREPGSTGLLAAVESPLGITRAVEIAHASERLIGI
ALGAEDYVRNLRTERSPEGTELLFARCAILQAARSAGIQAFDTVYSDANNEAGFLQEAAHIKQLGFDGKSLINPRQIELL
HNLYAPTRKEVAHARLVVEAAEAAAREGLGVVSLNGKMVDSPVIERARLVLSRAELSGIREE

Sequences:

>Translated_302_residues
MISASLQQRKTRTRRSMLFVPGANAAMVNNSFIYPADALMFDLEDSVALREKDAARRLVYHALQHPLYRDVETIVRVNAL
DSEWGVNDLEAVVRGGADVVRLPKTDTAQDVIDIENEILRIENACGREPGSTGLLAAVESPLGITRAVEIAHASERLIGI
ALGAEDYVRNLRTERSPEGTELLFARCAILQAARSAGIQAFDTVYSDANNEAGFLQEAAHIKQLGFDGKSLINPRQIELL
HNLYAPTRKEVAHARLVVEAAEAAAREGLGVVSLNGKMVDSPVIERARLVLSRAELSGIREE
>Mature_302_residues
MISASLQQRKTRTRRSMLFVPGANAAMVNNSFIYPADALMFDLEDSVALREKDAARRLVYHALQHPLYRDVETIVRVNAL
DSEWGVNDLEAVVRGGADVVRLPKTDTAQDVIDIENEILRIENACGREPGSTGLLAAVESPLGITRAVEIAHASERLIGI
ALGAEDYVRNLRTERSPEGTELLFARCAILQAARSAGIQAFDTVYSDANNEAGFLQEAAHIKQLGFDGKSLINPRQIELL
HNLYAPTRKEVAHARLVVEAAEAAAREGLGVVSLNGKMVDSPVIERARLVLSRAELSGIREE

Specific function: Represents a citryl-ACP lyase [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=310, Percent_Identity=25.1612903225806, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI87081764, Length=302, Percent_Identity=95.0331125827815, Blast_Score=559, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI17557458, Length=306, Percent_Identity=27.7777777777778, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR006475
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.6; =4.1.3.34 [H]

Molecular weight: Translated: 33016; Mature: 33016

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISASLQQRKTRTRRSMLFVPGANAAMVNNSFIYPADALMFDLEDSVALREKDAARRLVY
CCCCHHHHHHHHHHHCEEEECCCCEEEECCCEECCCHHEEEECCCHHHHHHHHHHHHHHH
HALQHPLYRDVETIVRVNALDSEWGVNDLEAVVRGGADVVRLPKTDTAQDVIDIENEILR
HHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHH
IENACGREPGSTGLLAAVESPLGITRAVEIAHASERLIGIALGAEDYVRNLRTERSPEGT
HHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCEEEEEECHHHHHHHHHCCCCCCHH
ELLFARCAILQAARSAGIQAFDTVYSDANNEAGFLQEAAHIKQLGFDGKSLINPRQIELL
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHCCHHHHHHH
HNLYAPTRKEVAHARLVVEAAEAAAREGLGVVSLNGKMVDSPVIERARLVLSRAELSGIR
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEECCHHHHHHHHHHHHHHHHCCCC
EE
CC
>Mature Secondary Structure
MISASLQQRKTRTRRSMLFVPGANAAMVNNSFIYPADALMFDLEDSVALREKDAARRLVY
CCCCHHHHHHHHHHHCEEEECCCCEEEECCCEECCCHHEEEECCCHHHHHHHHHHHHHHH
HALQHPLYRDVETIVRVNALDSEWGVNDLEAVVRGGADVVRLPKTDTAQDVIDIENEILR
HHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHH
IENACGREPGSTGLLAAVESPLGITRAVEIAHASERLIGIALGAEDYVRNLRTERSPEGT
HHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCEEEEEECHHHHHHHHHCCCCCCHH
ELLFARCAILQAARSAGIQAFDTVYSDANNEAGFLQEAAHIKQLGFDGKSLINPRQIELL
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHCCHHHHHHH
HNLYAPTRKEVAHARLVVEAAEAAAREGLGVVSLNGKMVDSPVIERARLVLSRAELSGIR
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEECCHHHHHHHHHHHHHHHHCCCC
EE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]