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The map label for this gene is lipA [H]

Identifier: 29142635

GI number: 29142635

Start: 2300481

End: 2301446

Strand: Direct

Name: lipA [H]

Synonym: t2234

Alternate gene names: 29142635

Gene position: 2300481-2301446 (Clockwise)

Preceding gene: 29142634

Following gene: 29142637

Centisome position: 48.01

GC content: 54.76

Gene sequence:

>966_bases
ATGAGTAAACCCATTGTGATGGAACGCGGTGTAAAATACCGCGATGCCGATAAGATGGCTCTTATCCCGGTTAAAAATGT
GGTCACAGAGCGCGACGCTCTACTACGTAAACCGGAATGGATGAAAATAAAACTTCCGGCGGATTCTACCCGTATCCAAG
GCATCAAAGCCGCAATGCGCAAAAATGGCCTGCATTCTGTCTGTGAGGAAGCGTCTTGCCCAAACCTGGCTGAATGTTTC
AACCACGGTACCGCTACGTTTATGATCCTTGGCGCCATTTGCACTCGCCGCTGTCCGTTCTGTGATGTCGCCCATGGCCG
CCCCGTTGCGCCGGATGCGGAAGAGCCGCAGAAACTGGCGCAAACGATCGCCGACATGGCGCTACGCTATGTGGTCATCA
CCTCTGTCGACCGTGACGATCTGCGTGACGGCGGCGCCCAGCACTTTGCTGACTGCATCACCGCCATCCGCGCCAAAAGC
CCGGAGATAAAAATAGAGACCCTGGTGCCAGACTTCCGTGGACGTATGGATCGCGCGCTGGATATTCTTAACGCGACGCC
GCCGGATGTGTTTAACCACAACCTGGAAAACGTGCCGCGTATTTACCGCCAGGTTCGTCCGGGCGCCGATTACAACTGGT
CGCTGAAATTACTGGAACGCTTCAAAGAAGCGCACCCGGAAATTCCGACAAAATCGGGTCTGATGGTGGGCTTAGGCGAA
ACCAATGCGGAAATCATTGAGGTTATGCGCGACTTACGCCGTCACGGCGTTACCATGCTGACGCTTGGTCAGTATCTACA
GCCAAGCCGCCATCACCTGCCGGTACAGCGTTATGTCAGCCCGGAAGAGTTTGACGAGATGAAAGCAGAAGCCCTGGCGA
TGGGCTTTACCCATGCCGCCTGTGGCCCCTTTGTACGTTCTTCGTACCATGCTGATCTCCAGGCCAAAGGCATGGAAGTG
AAATGA

Upstream 100 bases:

>100_bases
GATATACTGCGTGTCACAAATTCAAAAATAGTTGATAAATACAACTTTCCCTTGAATTGAAACGCTTTCCTTCGTTATTC
GCAACTGGAACACGCACGCT

Downstream 100 bases:

>100_bases
TCGTGTAATAGACGCTTACATTTCAGGCAAAAAAAAACCGGCAACTCGCCGGTTTTTTTACTCTGCGGAGCAAGCGCATT
GCGCTATTACTCTTTATGCG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSKPIVMERGVKYRDADKMALIPVKNVVTERDALLRKPEWMKIKLPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECF
NHGTATFMILGAICTRRCPFCDVAHGRPVAPDAEEPQKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIRAKS
PEIKIETLVPDFRGRMDRALDILNATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLLERFKEAHPEIPTKSGLMVGLGE
TNAEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQRYVSPEEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEV
K

Sequences:

>Translated_321_residues
MSKPIVMERGVKYRDADKMALIPVKNVVTERDALLRKPEWMKIKLPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECF
NHGTATFMILGAICTRRCPFCDVAHGRPVAPDAEEPQKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIRAKS
PEIKIETLVPDFRGRMDRALDILNATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLLERFKEAHPEIPTKSGLMVGLGE
TNAEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQRYVSPEEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEV
K
>Mature_320_residues
SKPIVMERGVKYRDADKMALIPVKNVVTERDALLRKPEWMKIKLPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFN
HGTATFMILGAICTRRCPFCDVAHGRPVAPDAEEPQKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIRAKSP
EIKIETLVPDFRGRMDRALDILNATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLLERFKEAHPEIPTKSGLMVGLGET
NAEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQRYVSPEEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=293, Percent_Identity=41.9795221843003, Blast_Score=248, Evalue=5e-66,
Organism=Homo sapiens, GI37577164, Length=258, Percent_Identity=41.8604651162791, Blast_Score=214, Evalue=7e-56,
Organism=Escherichia coli, GI1786846, Length=321, Percent_Identity=97.196261682243, Blast_Score=652, Evalue=0.0,
Organism=Caenorhabditis elegans, GI32564533, Length=290, Percent_Identity=41.3793103448276, Blast_Score=223, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6324770, Length=303, Percent_Identity=43.2343234323432, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI221513272, Length=284, Percent_Identity=45.0704225352113, Blast_Score=241, Evalue=5e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36042; Mature: 35911

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPIVMERGVKYRDADKMALIPVKNVVTERDALLRKPEWMKIKLPADSTRIQGIKAAMR
CCCCCCHHCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHH
KNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCDVAHGRPVAPDAEEPQKLA
HCCHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
QTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIRAKSPEIKIETLVPDFRGRMDRAL
HHHHHHHHHHHHEECCCHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHH
DILNATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLLERFKEAHPEIPTKSGLMVGLGE
HHHCCCCCHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECC
TNAEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQRYVSPEEFDEMKAEALAMGFTHAA
CCHHHHHHHHHHHHCCCEEEEHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHH
CGPFVRSSYHADLQAKGMEVK
HHHHHHCCCCCCHHCCCCCCC
>Mature Secondary Structure 
SKPIVMERGVKYRDADKMALIPVKNVVTERDALLRKPEWMKIKLPADSTRIQGIKAAMR
CCCCCHHCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHH
KNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCDVAHGRPVAPDAEEPQKLA
HCCHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
QTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIRAKSPEIKIETLVPDFRGRMDRAL
HHHHHHHHHHHHEECCCHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHH
DILNATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLLERFKEAHPEIPTKSGLMVGLGE
HHHCCCCCHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECC
TNAEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQRYVSPEEFDEMKAEALAMGFTHAA
CCHHHHHHHHHHHHCCCEEEEHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHH
CGPFVRSSYHADLQAKGMEVK
HHHHHHCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA