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The map label for this gene is rfbE [H]

Identifier: 29142563

GI number: 29142563

Start: 2217017

End: 2217727

Strand: Reverse

Name: rfbE [H]

Synonym: t2153

Alternate gene names: 29142563

Gene position: 2217727-2217017 (Counterclockwise)

Preceding gene: 29142564

Following gene: 29142562

Centisome position: 46.28

GC content: 39.1

Gene sequence:

>711_bases
ATGAAAATCTCTTGCAAAAACGTTGGCGTAATACTCCCTATATTTAACTCCAGCCACCGTTCATTTAAAAAAACTTTTTT
ACAGGCTGCTTCAGGTGGACGAATTGGCTCATCGAATACCGGGATTATTGAAGTAGAGGCATTAAAAAAAATTGATTTTA
CGCTTACCGAAGGTAACCGTCTTGCGCTTATCGGACACAATGGTTCGGGCAAAACAACTCTTCTGAGAGTACTTGCAGGG
GCCTATAAACCAACTTCAGGGAAGTATGAATGTATTGGTCGAGTCACCAGCCTCATTGATCCCATGATGGGAATGGATGG
TGAGCTTACCGGCCTGGAAAATATAAAACTGAGGGGACTATTTCTCGGTCTGTCAAAAAATGAAATAAAAAATATTACAG
AAGATGTCATCGAGTTCAGCGAGTTAGGTGATTTTATTAAAATCCCCGTTAGAACGTATTCTAGTGGTATGGTTCTGAGG
CTTGGTTTTTCTATTTCTACTGCAATTAATCCTGAAATTTTACTGATGGATGAATGGATGAGCGTGGGTGATAGTGATTT
TAAAAGAAAAGCAGAGATGAGATTAAATAGCTTTATCTCTAAAGCGGGCATTATGGTAATGGCCACTCATGATGATGAAC
TGGCTAAATCTGTATGTAATAAGTTCATCAGGCTTGAGCACAGTGAGATAGTTAGCAAAGGTGGATTTTAA

Upstream 100 bases:

>100_bases
TATATGGTTTTACACAATAATAACCTCTATTATTATGTTGATGGTCTCAACCTTAGTGTTGACTAAATATCGCTCAAGAA
TCGTATACTGGTTATAATTA

Downstream 100 bases:

>100_bases
TATTCTTATTGCAGTATTTATATCACTACGCGGTGAGAAATTTACTATTTTATAATAGCGTTACCCCAGATAATATAAAT
TTATAGGTTCGTTATGTATT

Product: polysaccharide export ABC transporter ATP-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MKISCKNVGVILPIFNSSHRSFKKTFLQAASGGRIGSSNTGIIEVEALKKIDFTLTEGNRLALIGHNGSGKTTLLRVLAG
AYKPTSGKYECIGRVTSLIDPMMGMDGELTGLENIKLRGLFLGLSKNEIKNITEDVIEFSELGDFIKIPVRTYSSGMVLR
LGFSISTAINPEILLMDEWMSVGDSDFKRKAEMRLNSFISKAGIMVMATHDDELAKSVCNKFIRLEHSEIVSKGGF

Sequences:

>Translated_236_residues
MKISCKNVGVILPIFNSSHRSFKKTFLQAASGGRIGSSNTGIIEVEALKKIDFTLTEGNRLALIGHNGSGKTTLLRVLAG
AYKPTSGKYECIGRVTSLIDPMMGMDGELTGLENIKLRGLFLGLSKNEIKNITEDVIEFSELGDFIKIPVRTYSSGMVLR
LGFSISTAINPEILLMDEWMSVGDSDFKRKAEMRLNSFISKAGIMVMATHDDELAKSVCNKFIRLEHSEIVSKGGF
>Mature_236_residues
MKISCKNVGVILPIFNSSHRSFKKTFLQAASGGRIGSSNTGIIEVEALKKIDFTLTEGNRLALIGHNGSGKTTLLRVLAG
AYKPTSGKYECIGRVTSLIDPMMGMDGELTGLENIKLRGLFLGLSKNEIKNITEDVIEFSELGDFIKIPVRTYSSGMVLR
LGFSISTAINPEILLMDEWMSVGDSDFKRKAEMRLNSFISKAGIMVMATHDDELAKSVCNKFIRLEHSEIVSKGGF

Specific function: May form an ATP-driven O-antigen export apparatus, in association with RfbD [H]

COG id: COG1134

COG function: function code GM; ABC-type polysaccharide/polyol phosphate transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI27477115, Length=147, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI27262624, Length=194, Percent_Identity=27.8350515463918, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI27262626, Length=194, Percent_Identity=27.8350515463918, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI153792144, Length=189, Percent_Identity=26.984126984127, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI6005701, Length=183, Percent_Identity=25.6830601092896, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI87081791, Length=200, Percent_Identity=26, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI87081782, Length=205, Percent_Identity=26.3414634146341, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787370, Length=203, Percent_Identity=25.615763546798, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1786703, Length=212, Percent_Identity=27.8301886792453, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI87081709, Length=223, Percent_Identity=23.3183856502242, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 25846; Mature: 25846

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISCKNVGVILPIFNSSHRSFKKTFLQAASGGRIGSSNTGIIEVEALKKIDFTLTEGNR
CEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEEECCCE
LALIGHNGSGKTTLLRVLAGAYKPTSGKYECIGRVTSLIDPMMGMDGELTGLENIKLRGL
EEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
FLGLSKNEIKNITEDVIEFSELGDFIKIPVRTYSSGMVLRLGFSISTAINPEILLMDEWM
EEECCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEECCEEECCCCCEEEEEECHH
SVGDSDFKRKAEMRLNSFISKAGIMVMATHDDELAKSVCNKFIRLEHSEIVSKGGF
CCCCHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHCCHHHHHHCCCC
>Mature Secondary Structure
MKISCKNVGVILPIFNSSHRSFKKTFLQAASGGRIGSSNTGIIEVEALKKIDFTLTEGNR
CEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEEECCCE
LALIGHNGSGKTTLLRVLAGAYKPTSGKYECIGRVTSLIDPMMGMDGELTGLENIKLRGL
EEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
FLGLSKNEIKNITEDVIEFSELGDFIKIPVRTYSSGMVLRLGFSISTAINPEILLMDEWM
EEECCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEECCEEECCCCCEEEEEECHH
SVGDSDFKRKAEMRLNSFISKAGIMVMATHDDELAKSVCNKFIRLEHSEIVSKGGF
CCCCHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7692217 [H]