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The map label for this gene is ybiS [H]

Identifier: 29142471

GI number: 29142471

Start: 2111748

End: 2112668

Strand: Direct

Name: ybiS [H]

Synonym: t2050

Alternate gene names: 29142471

Gene position: 2111748-2112668 (Clockwise)

Preceding gene: 29142469

Following gene: 29142474

Centisome position: 44.07

GC content: 55.27

Gene sequence:

>921_bases
ATGAATATGAAATTAACAACGCTTTTCGCGGCGGCTTTCGCTGTAGTTGGTTTTTGTAAAACAGCCTCAGCAGTCACTTA
CCCGCTGCCTACCGATGGTAGCCGTTTAATTGGGCAAAATCAGGTCATCACCGTTCCGGAGGGCAATACCCAGCCGCTGG
AGTATTTTGCCGCGGAGTATCAGATGGGGCTCTCCAACATGCTGGAAGCCAACCCTGGCGTGGATACCTTCCTGCCGAAG
GGCGGTACCGTGCTGAATATTCCGCAGCAGCTGATCCTGCCGGATACCGTACACGAAGGCATTATCATCAATAGCGCGGA
AATGCGCCTCTATTACTACCCGAAAGGCACAAACACCGTTATCGTCCTGCCGATTGGGATCGGTCAGTTAGGTAAAGACA
CGCCTATCAACTGGACGACCAAAGTTGAACGTAAAAAAGCCGGTCCGACCTGGACGCCTACGGCAAAAATGCACGCTGAA
TATCGCGCCGCCGGCGAGCCGCTGCCTGCCGTCGTGCCTGCGGGCCCGGATAACCCGATGGGCCTGTACGCGCTGTACAT
CGGTCGCCTGTATGCTATTCACGGCACCAACGCCAACTTCGGCATCGGGCTTCGCGTGAGCCACGGCTGCGTACGTCTGC
GCAACGACGATATCAAATTCCTGTTTGAAAATGTGCCGGTCGGTACCCGCGTGCAGTTTATCGATGAACCGGTAAAAGCC
ACCACCGAGCCGGACGGTAGCCGTTACATTGAGGTGCATAACCCGCTGTCAACGACGGAAGCGCAGTTTGAAGGTAAGGA
AGCGGTGCCGATCACGCTTAATAAGAGTATCCTGGCCGTCACCAACGAGCCTGACGTTGATCAGACCGTCGTACAACAGG
CAGTTCAGGATCGTTCCGGGATGCCGGTTCGTCTGAACTGA

Upstream 100 bases:

>100_bases
ACATCTGAATTCCCTGTTTACTGGAATGCACCTATAATGCGCTCCCAACATGACAGAATTCTCAACCTACTAGCCTGCTT
GGCACAGAATCTCGCATAAC

Downstream 100 bases:

>100_bases
CAGACTCTGCCTGCGAAGCCCGATAGACACTATCGGGCTTCTCTTACGGCATCAGTTGCAATAATCCATATCCCACCAGC
GCCGCCCATGCCAGCATCGG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MNMKLTTLFAAAFAVVGFCKTASAVTYPLPTDGSRLIGQNQVITVPEGNTQPLEYFAAEYQMGLSNMLEANPGVDTFLPK
GGTVLNIPQQLILPDTVHEGIIINSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNDDIKFLFENVPVGTRVQFIDEPVKA
TTEPDGSRYIEVHNPLSTTEAQFEGKEAVPITLNKSILAVTNEPDVDQTVVQQAVQDRSGMPVRLN

Sequences:

>Translated_306_residues
MNMKLTTLFAAAFAVVGFCKTASAVTYPLPTDGSRLIGQNQVITVPEGNTQPLEYFAAEYQMGLSNMLEANPGVDTFLPK
GGTVLNIPQQLILPDTVHEGIIINSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNDDIKFLFENVPVGTRVQFIDEPVKA
TTEPDGSRYIEVHNPLSTTEAQFEGKEAVPITLNKSILAVTNEPDVDQTVVQQAVQDRSGMPVRLN
>Mature_306_residues
MNMKLTTLFAAAFAVVGFCKTASAVTYPLPTDGSRLIGQNQVITVPEGNTQPLEYFAAEYQMGLSNMLEANPGVDTFLPK
GGTVLNIPQQLILPDTVHEGIIINSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNDDIKFLFENVPVGTRVQFIDEPVKA
TTEPDGSRYIEVHNPLSTTEAQFEGKEAVPITLNKSILAVTNEPDVDQTVVQQAVQDRSGMPVRLN

Specific function: Responsible, at least in part, for anchoring of the major outer membrane lipoprotein (Lpp) to the peptidoglycan via a meso-diaminopimelyl-L-Lys- bond on the terminal residue of Lpp [H]

COG id: COG1376

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the YkuD family [H]

Homologues:

Organism=Escherichia coli, GI1787040, Length=306, Percent_Identity=92.483660130719, Blast_Score=594, Evalue=1e-171,
Organism=Escherichia coli, GI1788299, Length=296, Percent_Identity=65.5405405405405, Blast_Score=407, Evalue=1e-115,
Organism=Escherichia coli, GI1787356, Length=282, Percent_Identity=52.1276595744681, Blast_Score=284, Evalue=6e-78,
Organism=Escherichia coli, GI1787968, Length=305, Percent_Identity=46.5573770491803, Blast_Score=244, Evalue=6e-66,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005490 [H]

Pfam domain/function: PF03734 YkuD [H]

EC number: NA

Molecular weight: Translated: 33293; Mature: 33293

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMKLTTLFAAAFAVVGFCKTASAVTYPLPTDGSRLIGQNQVITVPEGNTQPLEYFAAEY
CCEEHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEECCCCEEEECCCCCCCHHHHHHHHH
QMGLSNMLEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIIINSAEMRLYYYPKGTNTV
HHCHHHHHHCCCCCCEECCCCCEEEECCHHHCCCCCCCCCEEEECEEEEEEEEECCCCEE
IVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAEYRAAGEPLPAVVPAGPDNPM
EEEEECCCCCCCCCCCCCEEEEHHHCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCC
GLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNDDIKFLFENVPVGTRVQFIDEPVKA
HHHHEEEEEEEEEECCCCCEEEEEEEECCEEEEECCCEEEEEECCCCCCEEEEECCCCCC
TTEPDGSRYIEVHNPLSTTEAQFEGKEAVPITLNKSILAVTNEPDVDQTVVQQAVQDRSG
CCCCCCCEEEEECCCCCCCHHHCCCCCEEEEEECCEEEEEECCCCCHHHHHHHHHHCCCC
MPVRLN
CCEEEC
>Mature Secondary Structure
MNMKLTTLFAAAFAVVGFCKTASAVTYPLPTDGSRLIGQNQVITVPEGNTQPLEYFAAEY
CCEEHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEECCCCEEEECCCCCCCHHHHHHHHH
QMGLSNMLEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIIINSAEMRLYYYPKGTNTV
HHCHHHHHHCCCCCCEECCCCCEEEECCHHHCCCCCCCCCEEEECEEEEEEEEECCCCEE
IVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAEYRAAGEPLPAVVPAGPDNPM
EEEEECCCCCCCCCCCCCEEEEHHHCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCC
GLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNDDIKFLFENVPVGTRVQFIDEPVKA
HHHHEEEEEEEEEECCCCCEEEEEEEECCEEEEECCCEEEEEECCCCCCEEEEECCCCCC
TTEPDGSRYIEVHNPLSTTEAQFEGKEAVPITLNKSILAVTNEPDVDQTVVQQAVQDRSG
CCCCCCCEEEEECCCCCCCHHHCCCCCEEEEEECCEEEEEECCCCCHHHHHHHHHHCCCC
MPVRLN
CCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]