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The map label for this gene is ybjI [H]

Identifier: 29142450

GI number: 29142450

Start: 2086068

End: 2086883

Strand: Direct

Name: ybjI [H]

Synonym: t2029

Alternate gene names: 29142450

Gene position: 2086068-2086883 (Clockwise)

Preceding gene: 29142449

Following gene: 29142452

Centisome position: 43.53

GC content: 51.72

Gene sequence:

>816_bases
ATGAGTATTAAATTAATCGCCGTCGACATGGACGGCACTTTTCTGAGCGATCAAAAAACCTATAACCGTGACCGGTTTAT
GGCGCAGTACCAACAAATGAAGCGGCAAGGCATTCGCTTTGTCGTGGCCAGCGGCAACCAGTATTACCAGTTAATCTCCT
TCTTTCCGGAAATTGCGCACGAAATCGCTTTTGTCGCGGAAAACGGTGGCTGGGTGGTCAGCGAAGGAGAGGATGTTTTT
AACGGCGAGCTGGCAAAAGCCGACTTCCAGGCGGTTGTCGAACACCTGCTCACACGCGCCGATGTGGAGATCATCGCCTG
TGGAAAAAACAGCGCCTACACGCTGAAACGGTACGACGATGCGTTAAAAGCCGTTGCGGCAATGTATTATCATCGTCTGG
AGTTTGTGGATAATTTCAACAATATCAGCGATGTTTTTTTCAAATTCGGCCTTAATATTACCGATGAACGGATACCGGAA
GTACAGGCGGCGCTGCACGACGCCATTGGCGATATCATGGTCCCCGTTCATACCGGCTATGGCAGTATTGATTTAATAAT
TCCCGGCGTGCATAAAGCCAACGGCCTGCGTCTGTTACAGCAGCGCTGGGGCATTCATGATAGTGAAGTGGTGGTATTCG
GCGACGGCGGCAACGATATTGAGATGCTGCGTCAGGCCGGGTTTAGTTTTGCCATGTCGCACGCCAGCGAAGCGGTCGCG
GCGGCGGCGAAATACCGCGCCGGATCGAACAATCAGGAAGGCGTGCTGGATATCATCGACAGCGTATTAAACAACGAGCC
GCCGTTCAACGTCTAG

Upstream 100 bases:

>100_bases
CCATGATGGTTGTGCTGGCGCTGGTTATTCTGGCAGCGCTGGTGGCGAAGGCCGTCGCCAAACCTGTATCGACTCCGCAA
CCGGTGATGGAGCATAACGC

Downstream 100 bases:

>100_bases
CGGATGCCTCTCTACGCCTGATAACAGAAAGCTTATCAGGCGTAGCCGCTCTCTCAGACTGTTTGCAAATTCCCCGTCCG
CTTATCTTTTAAGAAGACCA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSIKLIAVDMDGTFLSDQKTYNRDRFMAQYQQMKRQGIRFVVASGNQYYQLISFFPEIAHEIAFVAENGGWVVSEGEDVF
NGELAKADFQAVVEHLLTRADVEIIACGKNSAYTLKRYDDALKAVAAMYYHRLEFVDNFNNISDVFFKFGLNITDERIPE
VQAALHDAIGDIMVPVHTGYGSIDLIIPGVHKANGLRLLQQRWGIHDSEVVVFGDGGNDIEMLRQAGFSFAMSHASEAVA
AAAKYRAGSNNQEGVLDIIDSVLNNEPPFNV

Sequences:

>Translated_271_residues
MSIKLIAVDMDGTFLSDQKTYNRDRFMAQYQQMKRQGIRFVVASGNQYYQLISFFPEIAHEIAFVAENGGWVVSEGEDVF
NGELAKADFQAVVEHLLTRADVEIIACGKNSAYTLKRYDDALKAVAAMYYHRLEFVDNFNNISDVFFKFGLNITDERIPE
VQAALHDAIGDIMVPVHTGYGSIDLIIPGVHKANGLRLLQQRWGIHDSEVVVFGDGGNDIEMLRQAGFSFAMSHASEAVA
AAAKYRAGSNNQEGVLDIIDSVLNNEPPFNV
>Mature_270_residues
SIKLIAVDMDGTFLSDQKTYNRDRFMAQYQQMKRQGIRFVVASGNQYYQLISFFPEIAHEIAFVAENGGWVVSEGEDVFN
GELAKADFQAVVEHLLTRADVEIIACGKNSAYTLKRYDDALKAVAAMYYHRLEFVDNFNNISDVFFKFGLNITDERIPEV
QAALHDAIGDIMVPVHTGYGSIDLIIPGVHKANGLRLLQQRWGIHDSEVVVFGDGGNDIEMLRQAGFSFAMSHASEAVAA
AAKYRAGSNNQEGVLDIIDSVLNNEPPFNV

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates FMN and beta-glucose 1-phosphate [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081790, Length=270, Percent_Identity=83.7037037037037, Blast_Score=472, Evalue=1e-134,
Organism=Escherichia coli, GI1787043, Length=270, Percent_Identity=52.962962962963, Blast_Score=298, Evalue=2e-82,
Organism=Escherichia coli, GI2367265, Length=266, Percent_Identity=24.0601503759398, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 30136; Mature: 30005

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKLIAVDMDGTFLSDQKTYNRDRFMAQYQQMKRQGIRFVVASGNQYYQLISFFPEIAH
CCEEEEEEECCCCEECCCHHCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHH
EIAFVAENGGWVVSEGEDVFNGELAKADFQAVVEHLLTRADVEIIACGKNSAYTLKRYDD
HHEEEECCCCEEEECCCHHHCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEHHHHHH
ALKAVAAMYYHRLEFVDNFNNISDVFFKFGLNITDERIPEVQAALHDAIGDIMVPVHTGY
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEECCC
GSIDLIIPGVHKANGLRLLQQRWGIHDSEVVVFGDGGNDIEMLRQAGFSFAMSHASEAVA
CCEEEEECCCCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHCCHHHHHHHHHHHHH
AAAKYRAGSNNQEGVLDIIDSVLNNEPPFNV
HHHHHCCCCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SIKLIAVDMDGTFLSDQKTYNRDRFMAQYQQMKRQGIRFVVASGNQYYQLISFFPEIAH
CEEEEEEECCCCEECCCHHCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHH
EIAFVAENGGWVVSEGEDVFNGELAKADFQAVVEHLLTRADVEIIACGKNSAYTLKRYDD
HHEEEECCCCEEEECCCHHHCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEHHHHHH
ALKAVAAMYYHRLEFVDNFNNISDVFFKFGLNITDERIPEVQAALHDAIGDIMVPVHTGY
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEECCC
GSIDLIIPGVHKANGLRLLQQRWGIHDSEVVVFGDGGNDIEMLRQAGFSFAMSHASEAVA
CCEEEEECCCCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHCCHHHHHHHHHHHHH
AAAKYRAGSNNQEGVLDIIDSVLNNEPPFNV
HHHHHCCCCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]