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The map label for this gene is rimK [H]

Identifier: 29142442

GI number: 29142442

Start: 2079201

End: 2080103

Strand: Reverse

Name: rimK [H]

Synonym: t2021

Alternate gene names: 29142442

Gene position: 2080103-2079201 (Counterclockwise)

Preceding gene: 29142443

Following gene: 29142441

Centisome position: 43.41

GC content: 56.37

Gene sequence:

>903_bases
GTGAAAATTGCCATATTGTCTAGGGATGGAACGCTCTATTCTTGCAAACGTCTGCGCGAAGCGGCGATGCGGCGCGGCCA
TCTGGTCGAGATACTCGATCCTCTTTCCTGCTATATGAATATTAATCCGGCAGCGTCGTCGATCCATTACAAGGGGCGTC
GTCTGCCCCATTTTGACGCCGTTATTCCCCGTATTGGTTCGGCCATTACCTTTTATGGTACCGCTGCGCTACGTCAGTTT
GAGCTACTGGGTAGCTATCCGCTGAATGAATCCGTCGCTATTACCCGCGCGCGCGATAAGCTGCGATCGCTACAACTTCT
GGCGCGCCAGGGGATCGATCTCCCCATTACCGGGATCGCCCATTCGCCGGATGATACCAGCGATCTTATTAAGATGGTTG
GCGGGGCGCCGCTGGTCGTGAAGCTGGTAGAAGGCACGCAAGGGATCGGCGTGGTATTGGCGGAGACGCGTCAGGCTGCT
GAAAGCGTTATTGATGCGTTTCGGGGGCTGAATGCCCATATCCTGGTTCAGGAATACATTGCCGAAGCTAAAGGATGCGA
TATTCGTTGTCTGGTCGTCGGCAATGAGGTTGTGGCGGCGATTGAGCGGTGCGCGAAAGCCGGTGATTTTCGCTCCAATC
TTCATCGTGGCGGGGTGGCCAGCATCGCGACGATCACGCCCCGTGAACGGGATATCGCCATCAAAGCCGCACAGACGCTG
GGGCTGGATGTGGCGGGTGTCGATATTTTACGCGCCGCGCGCGGGCCGCTGGTGATGGAGGTGAATGCCTCGCCGGGACT
GGAAGGTATCGAAAAGACTACGGGCGTGGATATCGCGGGGCGAATGATTCAGTGGATAGAACGTCACGCTACGCCTGAAT
TTTGCCTTAAAATCGGCGGCTAA

Upstream 100 bases:

>100_bases
TATCCTTGAATATTTGCATAAACAGGGCTGGGCGACGCGCTAAAACCTGCGGGCCAGTGTATGATACGCGGGCTTTTCAG
GGTTTTCAGCGAGGTGCAGG

Downstream 100 bases:

>100_bases
TCGCAATCGTGGATTCCGCCGTAGTACGAAGCACGCTTTTTGCGTAAGCTGTGCCGGTAATTTTTTCATCGTCATCGTAA
GAGGTAGCACATTATTATGA

Product: ribosomal protein S6 modification protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKIAILSRDGTLYSCKRLREAAMRRGHLVEILDPLSCYMNINPAASSIHYKGRRLPHFDAVIPRIGSAITFYGTAALRQF
ELLGSYPLNESVAITRARDKLRSLQLLARQGIDLPITGIAHSPDDTSDLIKMVGGAPLVVKLVEGTQGIGVVLAETRQAA
ESVIDAFRGLNAHILVQEYIAEAKGCDIRCLVVGNEVVAAIERCAKAGDFRSNLHRGGVASIATITPRERDIAIKAAQTL
GLDVAGVDILRAARGPLVMEVNASPGLEGIEKTTGVDIAGRMIQWIERHATPEFCLKIGG

Sequences:

>Translated_300_residues
MKIAILSRDGTLYSCKRLREAAMRRGHLVEILDPLSCYMNINPAASSIHYKGRRLPHFDAVIPRIGSAITFYGTAALRQF
ELLGSYPLNESVAITRARDKLRSLQLLARQGIDLPITGIAHSPDDTSDLIKMVGGAPLVVKLVEGTQGIGVVLAETRQAA
ESVIDAFRGLNAHILVQEYIAEAKGCDIRCLVVGNEVVAAIERCAKAGDFRSNLHRGGVASIATITPRERDIAIKAAQTL
GLDVAGVDILRAARGPLVMEVNASPGLEGIEKTTGVDIAGRMIQWIERHATPEFCLKIGG
>Mature_300_residues
MKIAILSRDGTLYSCKRLREAAMRRGHLVEILDPLSCYMNINPAASSIHYKGRRLPHFDAVIPRIGSAITFYGTAALRQF
ELLGSYPLNESVAITRARDKLRSLQLLARQGIDLPITGIAHSPDDTSDLIKMVGGAPLVVKLVEGTQGIGVVLAETRQAA
ESVIDAFRGLNAHILVQEYIAEAKGCDIRCLVVGNEVVAAIERCAKAGDFRSNLHRGGVASIATITPRERDIAIKAAQTL
GLDVAGVDILRAARGPLVMEVNASPGLEGIEKTTGVDIAGRMIQWIERHATPEFCLKIGG

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6 [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=207, Percent_Identity=31.4009661835749, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI134254462, Length=210, Percent_Identity=32.8571428571429, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1787076, Length=300, Percent_Identity=90.3333333333333, Blast_Score=560, Evalue=1e-161,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 32294; Mature: 32294

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAILSRDGTLYSCKRLREAAMRRGHLVEILDPLSCYMNINPAASSIHYKGRRLPHFDA
CEEEEEECCCCHHHHHHHHHHHHHCCCEEEHHCHHHHEEECCCHHHHHCCCCCCCCHHHH
VIPRIGSAITFYGTAALRQFELLGSYPLNESVAITRARDKLRSLQLLARQGIDLPITGIA
HHHHHCCEEEEHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCEECCC
HSPDDTSDLIKMVGGAPLVVKLVEGTQGIGVVLAETRQAAESVIDAFRGLNAHILVQEYI
CCCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHCCCCEEHHHHHH
AEAKGCDIRCLVVGNEVVAAIERCAKAGDFRSNLHRGGVASIATITPRERDIAIKAAQTL
HHHCCCCEEEEEECCHHHHHHHHHHHCCCHHHHHCCCCCEEEEEECCCCCCEEEEEHHHH
GLDVAGVDILRAARGPLVMEVNASPGLEGIEKTTGVDIAGRMIQWIERHATPEFCLKIGG
CCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCCHHHHHHHHHHHHHCCHHHEEECCC
>Mature Secondary Structure
MKIAILSRDGTLYSCKRLREAAMRRGHLVEILDPLSCYMNINPAASSIHYKGRRLPHFDA
CEEEEEECCCCHHHHHHHHHHHHHCCCEEEHHCHHHHEEECCCHHHHHCCCCCCCCHHHH
VIPRIGSAITFYGTAALRQFELLGSYPLNESVAITRARDKLRSLQLLARQGIDLPITGIA
HHHHHCCEEEEHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCEECCC
HSPDDTSDLIKMVGGAPLVVKLVEGTQGIGVVLAETRQAAESVIDAFRGLNAHILVQEYI
CCCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHCCCCEEHHHHHH
AEAKGCDIRCLVVGNEVVAAIERCAKAGDFRSNLHRGGVASIATITPRERDIAIKAAQTL
HHHCCCCEEEEEECCHHHHHHHHHHHCCCHHHHHCCCCCEEEEEECCCCCCEEEEEHHHH
GLDVAGVDILRAARGPLVMEVNASPGLEGIEKTTGVDIAGRMIQWIERHATPEFCLKIGG
CCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCCHHHHHHHHHHHHHCCHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA