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The map label for this gene is yflA [H]

Identifier: 29142356

GI number: 29142356

Start: 1973808

End: 1975241

Strand: Reverse

Name: yflA [H]

Synonym: t1931

Alternate gene names: 29142356

Gene position: 1975241-1973808 (Counterclockwise)

Preceding gene: 29142361

Following gene: 29142344

Centisome position: 41.22

GC content: 43.1

Gene sequence:

>1434_bases
ATGTTAGAGCAACTTTCACAACTCTTTGAATTTCTATGGGGTGGCCCATTATTTTTGTGTGTCATCGGCATTGGCTTTTA
TTTTACAGTACGCCTCAAATTTTTCCAGATTATCAATTTAAAAGAAATTTACCGTAATACAATTGGTACACTTGCCGGAA
AAAATAAACAAAACACTACTGGCGAGGCAGCGTCTAAAAAATCCTTAAAATCAATAGAGGTGGCTGCCACTGTGCTATCC
GGCTCATTAGGCGCGGGAACGATCGCAGGTGTTGCTGCGGCTATTGCGGTTGGCGGACCTGGGGCAATTTTCTGGATGTG
GATCATTGCGGTCGTGGGTATGATGACCAAAATGGTTGAAGTGACGCTGGCGGTGAAATACCGAAGTAAAGGTGAGAATG
GCGAATATTATGGCGGCCCAATGCATTATATTAAGAAAGAGCTCAATAAAAAATGGTATCCCCTGGCAGGATTATATGCG
TTTGCATTAATGATTCTGGTTATTACGGATGCCTGTTTTGTGCAAACTAATACGATGGCGGCGGTCATTCACTATACGTT
TGACATCCCAACAAGCGTCATTGGGGGATTTATTGTCATCGTAGGCGCGTTGGTTATTTTAAAAGGACTCGCCAGTCTTG
GTAAATTCTGTACGATTGCACTTCCACCGATCACCATTGCCTATTTCATTGGTGCCGCTGGTGTAGTGGTATTGAATATC
GAGGCTATCCCACAGGTTATAAAAAGTATTTTCTATTATGCCTTTGCCCCTGCGCCAGCAGCCGGTGGCTTTGTCGGGTC
AACCATCATGATGGCCATCAGTAAAGGAGCCAGTCGCGGAATATTCACTAACGAAGCCGGCATGGGTACCTCGGCCACAG
TACATGCTACTGCCAACGTGGATTATGCTTTCCGTCAGGGAATGTGGGGGGCAGTGGAAGTATTTTTTGTTTCAATGATT
ACCTGTAACTTTACCGCATTTGCTGTACTTGCTTCTGGTATGTGGACAGACGCCAGTTATCAAGGTATTCAGATTATCTT
TGCCGCCTTAAAAGAAACATGGCATCCGATTATTGTTCAGGTTCTCTGTCTCGGTGTGGCGTTGATTCTGTTTACTTCTT
ATCTTGGTTCATATATTAAATTTCGCACTTCAATAAACTATATCTTTGGCGATAAGCTAGAACGCATCATTAAATGGCTA
TATTTTTTGCCGCCACTGATTGCCGTTAATATGGAAATCCCGGTAATCTGGTTAATGGCTGATATTGCTGTCGGTTTTCT
GGTTATCCCCAACGTAATTGCTCTGTTCTTGCTGCGTAAAGAATTTATTAGCGAATTCAATTTATTCAGAACGCGTACAC
AGCGGGACACTAATTCTGAAAAAACAACGCAAATCACGCACGTAAATATGTCGAAGAGTGAAGGGGAAGAATAG

Upstream 100 bases:

>100_bases
CTGACTTTCCAGTTTAGTGTTGTTGGCAAAATTCAGGATTACGCTGAACTGGAAAGATTATTTCTGAGACTGAACGAATT
AATTTAAATCCGAGGTCATT

Downstream 100 bases:

>100_bases
TCACCATCGCGGCCAGAACAGGTAAGATGGTCAGAAGAGTAACAAAGCGCCCACTTTCCGGGCGCTTCGCACAGGCATGG
TTAACTTGCTTTACGCACCT

Product: ion:amino acid symporter

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MLEQLSQLFEFLWGGPLFLCVIGIGFYFTVRLKFFQIINLKEIYRNTIGTLAGKNKQNTTGEAASKKSLKSIEVAATVLS
GSLGAGTIAGVAAAIAVGGPGAIFWMWIIAVVGMMTKMVEVTLAVKYRSKGENGEYYGGPMHYIKKELNKKWYPLAGLYA
FALMILVITDACFVQTNTMAAVIHYTFDIPTSVIGGFIVIVGALVILKGLASLGKFCTIALPPITIAYFIGAAGVVVLNI
EAIPQVIKSIFYYAFAPAPAAGGFVGSTIMMAISKGASRGIFTNEAGMGTSATVHATANVDYAFRQGMWGAVEVFFVSMI
TCNFTAFAVLASGMWTDASYQGIQIIFAALKETWHPIIVQVLCLGVALILFTSYLGSYIKFRTSINYIFGDKLERIIKWL
YFLPPLIAVNMEIPVIWLMADIAVGFLVIPNVIALFLLRKEFISEFNLFRTRTQRDTNSEKTTQITHVNMSKSEGEE

Sequences:

>Translated_477_residues
MLEQLSQLFEFLWGGPLFLCVIGIGFYFTVRLKFFQIINLKEIYRNTIGTLAGKNKQNTTGEAASKKSLKSIEVAATVLS
GSLGAGTIAGVAAAIAVGGPGAIFWMWIIAVVGMMTKMVEVTLAVKYRSKGENGEYYGGPMHYIKKELNKKWYPLAGLYA
FALMILVITDACFVQTNTMAAVIHYTFDIPTSVIGGFIVIVGALVILKGLASLGKFCTIALPPITIAYFIGAAGVVVLNI
EAIPQVIKSIFYYAFAPAPAAGGFVGSTIMMAISKGASRGIFTNEAGMGTSATVHATANVDYAFRQGMWGAVEVFFVSMI
TCNFTAFAVLASGMWTDASYQGIQIIFAALKETWHPIIVQVLCLGVALILFTSYLGSYIKFRTSINYIFGDKLERIIKWL
YFLPPLIAVNMEIPVIWLMADIAVGFLVIPNVIALFLLRKEFISEFNLFRTRTQRDTNSEKTTQITHVNMSKSEGEE
>Mature_477_residues
MLEQLSQLFEFLWGGPLFLCVIGIGFYFTVRLKFFQIINLKEIYRNTIGTLAGKNKQNTTGEAASKKSLKSIEVAATVLS
GSLGAGTIAGVAAAIAVGGPGAIFWMWIIAVVGMMTKMVEVTLAVKYRSKGENGEYYGGPMHYIKKELNKKWYPLAGLYA
FALMILVITDACFVQTNTMAAVIHYTFDIPTSVIGGFIVIVGALVILKGLASLGKFCTIALPPITIAYFIGAAGVVVLNI
EAIPQVIKSIFYYAFAPAPAAGGFVGSTIMMAISKGASRGIFTNEAGMGTSATVHATANVDYAFRQGMWGAVEVFFVSMI
TCNFTAFAVLASGMWTDASYQGIQIIFAALKETWHPIIVQVLCLGVALILFTSYLGSYIKFRTSINYIFGDKLERIIKWL
YFLPPLIAVNMEIPVIWLMADIAVGFLVIPNVIALFLLRKEFISEFNLFRTRTQRDTNSEKTTQITHVNMSKSEGEE

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=434, Percent_Identity=30.184331797235, Blast_Score=185, Evalue=6e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 52089; Mature: 52089

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEQLSQLFEFLWGGPLFLCVIGIGFYFTVRLKFFQIINLKEIYRNTIGTLAGKNKQNTT
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GEAASKKSLKSIEVAATVLSGSLGAGTIAGVAAAIAVGGPGAIFWMWIIAVVGMMTKMVE
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
VTLAVKYRSKGENGEYYGGPMHYIKKELNKKWYPLAGLYAFALMILVITDACFVQTNTMA
HHHEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEECCCEE
AVIHYTFDIPTSVIGGFIVIVGALVILKGLASLGKFCTIALPPITIAYFIGAAGVVVLNI
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEEH
EAIPQVIKSIFYYAFAPAPAAGGFVGSTIMMAISKGASRGIFTNEAGMGTSATVHATANV
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEECCCCCCCCEEEEEECCC
DYAFRQGMWGAVEVFFVSMITCNFTAFAVLASGMWTDASYQGIQIIFAALKETWHPIIVQ
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VLCLGVALILFTSYLGSYIKFRTSINYIFGDKLERIIKWLYFLPPLIAVNMEIPVIWLMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEECCCCCHHHHHH
DIAVGFLVIPNVIALFLLRKEFISEFNLFRTRTQRDTNSEKTTQITHVNMSKSEGEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure
MLEQLSQLFEFLWGGPLFLCVIGIGFYFTVRLKFFQIINLKEIYRNTIGTLAGKNKQNTT
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GEAASKKSLKSIEVAATVLSGSLGAGTIAGVAAAIAVGGPGAIFWMWIIAVVGMMTKMVE
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
VTLAVKYRSKGENGEYYGGPMHYIKKELNKKWYPLAGLYAFALMILVITDACFVQTNTMA
HHHEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEECCCEE
AVIHYTFDIPTSVIGGFIVIVGALVILKGLASLGKFCTIALPPITIAYFIGAAGVVVLNI
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEEH
EAIPQVIKSIFYYAFAPAPAAGGFVGSTIMMAISKGASRGIFTNEAGMGTSATVHATANV
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEECCCCCCCCEEEEEECCC
DYAFRQGMWGAVEVFFVSMITCNFTAFAVLASGMWTDASYQGIQIIFAALKETWHPIIVQ
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VLCLGVALILFTSYLGSYIKFRTSINYIFGDKLERIIKWLYFLPPLIAVNMEIPVIWLMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEECCCCCHHHHHH
DIAVGFLVIPNVIALFLLRKEFISEFNLFRTRTQRDTNSEKTTQITHVNMSKSEGEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377 [H]