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The map label for this gene is htrB [H]

Identifier: 29142197

GI number: 29142197

Start: 1827371

End: 1828291

Strand: Direct

Name: htrB [H]

Synonym: t1765

Alternate gene names: 29142197

Gene position: 1827371-1828291 (Clockwise)

Preceding gene: 29142195

Following gene: 29142198

Centisome position: 38.13

GC content: 54.83

Gene sequence:

>921_bases
ATGACGAAGTTGCCTAAGTTCTCCGTGGCCTTACTGCACCCGCGCTATTGGTTAACCTGGTTGGGTATAGGCGCGCTTTG
GTTGGTCGTGCAACTCCCCTACCCGGTCATCTATAAATTGGGTTGCGCATTGGGCCACCTGGCGCGACGCGTAATGAAAC
GCCGCGCGAAAATCGCCTATCGCAACCTTGAATTATGCTTTCCGGAAATGAGCGCGCAGGAACGCCACACCATGGTGGTC
AAAAACTTTGAATCCGTCGGTATGGGCGTGATGGAAACCGGCATGGCCTGGTTCTGGCCCGATCGGCGAGTGAACCGCTG
GATGGAAGCGAGCGGTCTGGAGCATATCCGTGAAGTTAAGGCGCAGGGGCTGGGGTTCATTCTGGTAGGAATACATTTCC
TTACCCTTGAGTTTGGCGCCCGCATGTTTGGTATGCATAACCCGGGTATCGGCGTTTATCGCCCGAATGATAATCCGTTG
CTCGACTGGTTACAGACCTGGGGGCGCTTACGCTCCAATAAATCGATGTTGGATCGCAAAGATCTGAAAGGTATGGTTAA
GGCATTGAAAAGCGGCGAATTGATCTGGTATGCGCCGGATCACGATTATGGCCCGCGCGCCAGCGTCTTTGTACCGTTAT
TCGCCGTCGACCAGGCCGCTACGACCTCCGGTACCTGGATGCTCGCGCGCATGTCTAAAGCCTGTATTATCCCCTCCGTG
CCTCGCCGTAAACCCGACGGGAAAGGCTACGAACTGATCATCCTTCCTGCGGAATATTCGCCTCCGCTGGAGAGCGCAGA
AGCGACTGCCGCATGGATGAATAAGATTGTTGAGCAGTGCATTATGATGGCGCCAGAGCAGTATATGTGGCTGCATCGCC
GCTTTAAAACTCGCCCTGAAGGCGTACCGTCGCGCTATTGA

Upstream 100 bases:

>100_bases
ACAATTGTCGATTTAATCGCCACTCTCCTGCTACATATTTTTGCTCAACGCAAAAAGATGCGAGAATACGGAGAATTGTT
CGTTGAAAGACAGGATAGAA

Downstream 100 bases:

>100_bases
CAGGATTGCAGCCGTCAGGCTGCAATTTTTCCCCTCTTTTAGCTTTAAAAGCTCCCCACATTGCGAGGCTGGTAAGGTCG
GCGCATAATTATAATTAGTA

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MTKLPKFSVALLHPRYWLTWLGIGALWLVVQLPYPVIYKLGCALGHLARRVMKRRAKIAYRNLELCFPEMSAQERHTMVV
KNFESVGMGVMETGMAWFWPDRRVNRWMEASGLEHIREVKAQGLGFILVGIHFLTLEFGARMFGMHNPGIGVYRPNDNPL
LDWLQTWGRLRSNKSMLDRKDLKGMVKALKSGELIWYAPDHDYGPRASVFVPLFAVDQAATTSGTWMLARMSKACIIPSV
PRRKPDGKGYELIILPAEYSPPLESAEATAAWMNKIVEQCIMMAPEQYMWLHRRFKTRPEGVPSRY

Sequences:

>Translated_306_residues
MTKLPKFSVALLHPRYWLTWLGIGALWLVVQLPYPVIYKLGCALGHLARRVMKRRAKIAYRNLELCFPEMSAQERHTMVV
KNFESVGMGVMETGMAWFWPDRRVNRWMEASGLEHIREVKAQGLGFILVGIHFLTLEFGARMFGMHNPGIGVYRPNDNPL
LDWLQTWGRLRSNKSMLDRKDLKGMVKALKSGELIWYAPDHDYGPRASVFVPLFAVDQAATTSGTWMLARMSKACIIPSV
PRRKPDGKGYELIILPAEYSPPLESAEATAAWMNKIVEQCIMMAPEQYMWLHRRFKTRPEGVPSRY
>Mature_305_residues
TKLPKFSVALLHPRYWLTWLGIGALWLVVQLPYPVIYKLGCALGHLARRVMKRRAKIAYRNLELCFPEMSAQERHTMVVK
NFESVGMGVMETGMAWFWPDRRVNRWMEASGLEHIREVKAQGLGFILVGIHFLTLEFGARMFGMHNPGIGVYRPNDNPLL
DWLQTWGRLRSNKSMLDRKDLKGMVKALKSGELIWYAPDHDYGPRASVFVPLFAVDQAATTSGTWMLARMSKACIIPSVP
RRKPDGKGYELIILPAEYSPPLESAEATAAWMNKIVEQCIMMAPEQYMWLHRRFKTRPEGVPSRY

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=306, Percent_Identity=83.3333333333333, Blast_Score=546, Evalue=1e-157,
Organism=Escherichia coli, GI87082094, Length=301, Percent_Identity=53.156146179402, Blast_Score=349, Evalue=1e-97,
Organism=Escherichia coli, GI1788161, Length=306, Percent_Identity=29.7385620915033, Blast_Score=115, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 35154; Mature: 35023

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLPKFSVALLHPRYWLTWLGIGALWLVVQLPYPVIYKLGCALGHLARRVMKRRAKIAY
CCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLELCFPEMSAQERHTMVVKNFESVGMGVMETGMAWFWPDRRVNRWMEASGLEHIREVK
HHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHH
AQGLGFILVGIHFLTLEFGARMFGMHNPGIGVYRPNDNPLLDWLQTWGRLRSNKSMLDRK
HCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHCCHHHHHHH
DLKGMVKALKSGELIWYAPDHDYGPRASVFVPLFAVDQAATTSGTWMLARMSKACIIPSV
HHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHCCCCCCCC
PRRKPDGKGYELIILPAEYSPPLESAEATAAWMNKIVEQCIMMAPEQYMWLHRRFKTRPE
CCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
GVPSRY
CCCCCC
>Mature Secondary Structure 
TKLPKFSVALLHPRYWLTWLGIGALWLVVQLPYPVIYKLGCALGHLARRVMKRRAKIAY
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLELCFPEMSAQERHTMVVKNFESVGMGVMETGMAWFWPDRRVNRWMEASGLEHIREVK
HHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHH
AQGLGFILVGIHFLTLEFGARMFGMHNPGIGVYRPNDNPLLDWLQTWGRLRSNKSMLDRK
HCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHCCHHHHHHH
DLKGMVKALKSGELIWYAPDHDYGPRASVFVPLFAVDQAATTSGTWMLARMSKACIIPSV
HHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHCCCCCCCC
PRRKPDGKGYELIILPAEYSPPLESAEATAAWMNKIVEQCIMMAPEQYMWLHRRFKTRPE
CCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
GVPSRY
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]