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The map label for this gene is narX [H]

Identifier: 29142109

GI number: 29142109

Start: 1739224

End: 1741020

Strand: Direct

Name: narX [H]

Synonym: t1676

Alternate gene names: 29142109

Gene position: 1739224-1741020 (Clockwise)

Preceding gene: 29142101

Following gene: 29142110

Centisome position: 36.29

GC content: 54.09

Gene sequence:

>1797_bases
ATGTTTAAACGGTGTTTCTCTCCTCTTACGCTGGTTAACCAACTGGCGCTGATTGTCATGCTCTCTACCGCTATCGGCGT
GGCGGGGATGGCGGTTTCCGGCTGGCTGGTTCAGGGCGTGCAGGGTAGCGCTCATGCGATTAATAAAGCGGGCTCATTGC
GTATGCAAAGCTATCGACTACTGGCCGCTGTCCCGCTGGATGCTAAAGATCAAAAGCTGCTGGATGAGATGGAGCAAACG
GCATTCAGCCCTGAACTGACCCGCGCCGCTGAGCGAGACGGGCAGCAGAAGCAGCTTAAAGCGTTGCAGGATTACTGGCA
TAACGAACTCTCGCCAAGGCTACAGCACGCGCAAAACGCGCACGCGGTCGCAGAAGATATCACGCGCTTTGTGGCGGGCC
TGGATCGCCTGGTGACGTCGTTCGACCATACGACTGAATTGCGTATCGAACGAGTGGTATTAGTTCACCGGGTAATGGCG
ATCTTTATGGCGCTATTGCTGGTATTTACGATTATCTGGCTACGTGTTCGCCTTTTGCAGCCCTGGAAACAGTTGTTATC
AATGGCGCGCGCCGTCAGTCAGCGCGACTTTACCCAACGTGCCAATATCAGTGGACGCAATGAGATGGCCGCGTTGGGTT
CTGCGCTCAATAATATGTCTGAAGAACTGGCCGAAAGTTATGCAGTACTGGAACAGCGAGTACAGGAAAAAACAGCCGGT
CTGGAACATAAAAACCAGATTCTCTCGTTTTTGTGGCAAGCCAACCGCCGACTGCATTCACAGGCTCCGCTATGTGAACG
TCTTTCACCGGTACTGAATGGCCTGCAAAATTTAACCCAGTTGCATGATATTGAGCTGCGAGTCTATGACCTGGAAGATG
AAGATAATCACCAGGAATTTACCTGTCAGTCGGACATCAGTTGCGATGATAAAGGCTGCCACCTTTGCCCACGTAGCGCC
CTGCCGATGATTAACGGCGGCACCACCTTAAAATGGCGGCTCACCGACTCTCACACCCAATACGGTATTTTGCTGGCGAC
GCTGCCCTATGGACGCCATCTCAGCCACGATCAGCAACAGTTGGTGGACACGCTGGTTGAACAGCTTACCGCGACTCTGG
CGCTCGACAGGCATCAGGAGCGCCAGCAGCGGTTGATTGTCATGGAAGAGCGTGCCACCATTGCCCGCGAACTGCATGAT
TCTATTGCCCAGTCGCTCTCTTGTATGAAGATGCAGGTGAGCTGCCTGCAAATGCAGGGCGACGCGTTACCGGAAAGCAG
CCGTGAGTTACTCAGCCAAATTCGCAATGAGCTGAACTGCTCCTGGGCGCAACTGCGCGAACTGTTAACCACTTTCCGCT
TACAGTTGACGGAACCCGGGCTACGTCCGGCGCTGGAGGCAAGCTGCCAGGAGTTCAGCGCCCGGTTTGGCTTTACGGTA
AAACTGGATTATCAGCTTCCCCCCCGCCTGGTGCCATCACACCAGGCGATACACCTGTTGCAAATTGCCAGGGAAGCCCC
CAGCAACGCCCTTAAACACTCTCATGCCGACGATGTGGTGGTGACAGTGACGCAATGTGGCAAACAGGTCAAACTAAAAG
TGCAGGACAATGGCTGCGGCGTCCCGGAAAACGCCGAACGCAGTAATCACTACGGCATGATCATCATGCGCGATCGGGCG
CAAAGTTTGCGCGGCGATTGCCAGGTACGCCGACGCGAGACTGGCGGAACAGAGGTCACTGTTACTTTTATTCCAGAAAC
AAACTTCACAGAAACCCAGGGAGATACCCATGAATAA

Upstream 100 bases:

>100_bases
GTATACTCCGCTGTATCGTTGAGAATAGCGGCACAGCCGATTGACGCCCCCTTTTTTGCTACGTTTTTTCGGCGACATTA
CCCCGAAGAAAGAAGGTAAC

Downstream 100 bases:

>100_bases
TCAGGAACCGGCAACCATCCTGTTAATCGATGACCATCCGATGCTACGCACGGGTGTAAAGCAGCTTGTGAGTATGGCGC
CCGATATCAGCGTGGTCGGA

Product: nitrate/nitrite sensor protein NarX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MFKRCFSPLTLVNQLALIVMLSTAIGVAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRLLAAVPLDAKDQKLLDEMEQT
AFSPELTRAAERDGQQKQLKALQDYWHNELSPRLQHAQNAHAVAEDITRFVAGLDRLVTSFDHTTELRIERVVLVHRVMA
IFMALLLVFTIIWLRVRLLQPWKQLLSMARAVSQRDFTQRANISGRNEMAALGSALNNMSEELAESYAVLEQRVQEKTAG
LEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQNLTQLHDIELRVYDLEDEDNHQEFTCQSDISCDDKGCHLCPRSA
LPMINGGTTLKWRLTDSHTQYGILLATLPYGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQRLIVMEERATIARELHD
SIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNCSWAQLRELLTTFRLQLTEPGLRPALEASCQEFSARFGFTV
KLDYQLPPRLVPSHQAIHLLQIAREAPSNALKHSHADDVVVTVTQCGKQVKLKVQDNGCGVPENAERSNHYGMIIMRDRA
QSLRGDCQVRRRETGGTEVTVTFIPETNFTETQGDTHE

Sequences:

>Translated_598_residues
MFKRCFSPLTLVNQLALIVMLSTAIGVAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRLLAAVPLDAKDQKLLDEMEQT
AFSPELTRAAERDGQQKQLKALQDYWHNELSPRLQHAQNAHAVAEDITRFVAGLDRLVTSFDHTTELRIERVVLVHRVMA
IFMALLLVFTIIWLRVRLLQPWKQLLSMARAVSQRDFTQRANISGRNEMAALGSALNNMSEELAESYAVLEQRVQEKTAG
LEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQNLTQLHDIELRVYDLEDEDNHQEFTCQSDISCDDKGCHLCPRSA
LPMINGGTTLKWRLTDSHTQYGILLATLPYGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQRLIVMEERATIARELHD
SIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNCSWAQLRELLTTFRLQLTEPGLRPALEASCQEFSARFGFTV
KLDYQLPPRLVPSHQAIHLLQIAREAPSNALKHSHADDVVVTVTQCGKQVKLKVQDNGCGVPENAERSNHYGMIIMRDRA
QSLRGDCQVRRRETGGTEVTVTFIPETNFTETQGDTHE
>Mature_598_residues
MFKRCFSPLTLVNQLALIVMLSTAIGVAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRLLAAVPLDAKDQKLLDEMEQT
AFSPELTRAAERDGQQKQLKALQDYWHNELSPRLQHAQNAHAVAEDITRFVAGLDRLVTSFDHTTELRIERVVLVHRVMA
IFMALLLVFTIIWLRVRLLQPWKQLLSMARAVSQRDFTQRANISGRNEMAALGSALNNMSEELAESYAVLEQRVQEKTAG
LEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQNLTQLHDIELRVYDLEDEDNHQEFTCQSDISCDDKGCHLCPRSA
LPMINGGTTLKWRLTDSHTQYGILLATLPYGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQRLIVMEERATIARELHD
SIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNCSWAQLRELLTTFRLQLTEPGLRPALEASCQEFSARFGFTV
KLDYQLPPRLVPSHQAIHLLQIAREAPSNALKHSHADDVVVTVTQCGKQVKLKVQDNGCGVPENAERSNHYGMIIMRDRA
QSLRGDCQVRRRETGGTEVTVTFIPETNFTETQGDTHE

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate availability to the narL protein and of both nitrate/nitrite to the narP protein. NarX probably activates narL and narP by phosphorylation in the presence of nitrate. NarX also plays a

COG id: COG3850

COG function: function code T; Signal transduction histidine kinase, nitrate/nitrite-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=598, Percent_Identity=85.7859531772575, Blast_Score=1060, Evalue=0.0,
Organism=Escherichia coli, GI1788812, Length=583, Percent_Identity=30.188679245283, Blast_Score=191, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR003661
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 67536; Mature: 67536

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKRCFSPLTLVNQLALIVMLSTAIGVAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRL
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHE
LAAVPLDAKDQKLLDEMEQTAFSPELTRAAERDGQQKQLKALQDYWHNELSPRLQHAQNA
EEECCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
HAVAEDITRFVAGLDRLVTSFDHTTELRIERVVLVHRVMAIFMALLLVFTIIWLRVRLLQ
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PWKQLLSMARAVSQRDFTQRANISGRNEMAALGSALNNMSEELAESYAVLEQRVQEKTAG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQNLTQLHDIELRVYDLEDEDNHQEF
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCE
TCQSDISCDDKGCHLCPRSALPMINGGTTLKWRLTDSHTQYGILLATLPYGRHLSHDQQQ
EECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCHHHH
LVDTLVEQLTATLALDRHQERQQRLIVMEERATIARELHDSIAQSLSCMKMQVSCLQMQG
HHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DALPESSRELLSQIRNELNCSWAQLRELLTTFRLQLTEPGLRPALEASCQEFSARFGFTV
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEE
KLDYQLPPRLVPSHQAIHLLQIAREAPSNALKHSHADDVVVTVTQCGKQVKLKVQDNGCG
EECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCCCEEEEHHHCCCEEEEEECCCCCC
VPENAERSNHYGMIIMRDRAQSLRGDCQVRRRETGGTEVTVTFIPETNFTETQGDTHE
CCCCCCCCCCCCEEEECCCHHHHHCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MFKRCFSPLTLVNQLALIVMLSTAIGVAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRL
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHE
LAAVPLDAKDQKLLDEMEQTAFSPELTRAAERDGQQKQLKALQDYWHNELSPRLQHAQNA
EEECCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
HAVAEDITRFVAGLDRLVTSFDHTTELRIERVVLVHRVMAIFMALLLVFTIIWLRVRLLQ
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PWKQLLSMARAVSQRDFTQRANISGRNEMAALGSALNNMSEELAESYAVLEQRVQEKTAG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQNLTQLHDIELRVYDLEDEDNHQEF
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCE
TCQSDISCDDKGCHLCPRSALPMINGGTTLKWRLTDSHTQYGILLATLPYGRHLSHDQQQ
EECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCHHHH
LVDTLVEQLTATLALDRHQERQQRLIVMEERATIARELHDSIAQSLSCMKMQVSCLQMQG
HHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DALPESSRELLSQIRNELNCSWAQLRELLTTFRLQLTEPGLRPALEASCQEFSARFGFTV
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEE
KLDYQLPPRLVPSHQAIHLLQIAREAPSNALKHSHADDVVVTVTQCGKQVKLKVQDNGCG
EECCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCCCEEEEHHHCCCEEEEEECCCCCC
VPENAERSNHYGMIIMRDRAQSLRGDCQVRRRETGGTEVTVTFIPETNFTETQGDTHE
CCCCCCCCCCCCEEEECCCHHHHHCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]