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The map label for this gene is nemA [H]

Identifier: 29141763

GI number: 29141763

Start: 1367331

End: 1368428

Strand: Reverse

Name: nemA [H]

Synonym: t1304

Alternate gene names: 29141763

Gene position: 1368428-1367331 (Counterclockwise)

Preceding gene: 29141764

Following gene: 29141762

Centisome position: 28.56

GC content: 58.11

Gene sequence:

>1098_bases
ATGTCATCAGCAAAACTGTTTACCCCCCTCAAGGTGGGTGCCATCACGGCGACAAATCGCGTATTTATGGCGCCTTTGAC
GCGTTTGCGGAGTATTGAACCGGGCGACATTCCTACGCCGTTAATGGCGGAATATTACCGTCAACGCGCCAGCGCCGGAT
TGATCATCAGCGAAGCCACACAAATCTCCGCGCAGGCGAAAGGCTATGCCGGCGCACCGGGATTACACAGCGATGAGCAG
ATCGCCGCCTGGAAAAAAATCACTCAGGGAGTCCATGCCCAGGGCGGCCATATGGCGGTGCAACTGTGGCATACGGGCCG
TATTTCACACGCCAGCCTGCAACCAGGCGGACAAGCGCCCGTCGCGCCATCCGCTATCAATGCGGGAACCCGCACCTCTT
TGCGTGACGAAAACGGCCAGGCCATCCGCGTAGAAACTTCCACGCCGCGCGCGCTGGATACTCATGAGATCCCCGGGATC
GTCAACGATTTCCGCCAGGCTATCGCCAATGCCCGAGAGGCAGGCTTCGATCTGGTGGAGCTGCACTCCGCGCACGGTTA
TCTGCTGCACCAGTTCCTCTCACCGTCTTCTAATCACCGTACCGATCGGTACGGCGGCAGCGTAGAGAATCGCGCCCGTC
TGGTGCTGGAGGTGGTTGATGCCGGCATTAAGGAATGGGGCGCGGACCGTATCGGCATCCGCCTTTCTCCTGTCGGCACT
TTCCAGAATGTCGATAACGGCCCGAATGAAGAAGCTGACGCCCTTTATCTGATTGAAGCGTTAGGCAAACGCGGCATCGC
TTATCTGCATATGTCTGAACCAGACTGGGCGGGCGGCGAACCGTACAGCGACGCGTTCCGTGAAAAAGTGCGCGCGCGTT
TCCACGGTCCCATCATCGGCGCTGGCGCCTATACGCCAGAAAAAGCGGAAGATCTGATCGAAAAAGGGCTGATTGACGCC
GTGGCGTTTGGTCGCGATTACATCGCTAACCCGGATCTGGTGGCGCGTCTGCAACGTAAAGCGGAACTTAATCCACAGCG
TCCCGAAAGTTTTTATGGCGGCGGCGCGGAAGGCTACACTGACTACCCTACTCTGTAA

Upstream 100 bases:

>100_bases
TTGGCGCATGTTAAGACCATCATTGCAACGCCTGAGCAGTAACGGGCGTTTTTATTTCCTTTATTACAAGACGACCGGTC
TACTTATATCAGGAGCCACT

Downstream 100 bases:

>100_bases
TTTCATTGATAGCGGCCTCACATCGCCGCTATACTTTGAATATCTTGACTATTTTAAGGACAACGAAAATGCGTTTATTG
CATACTATGCTGCGCGTCGG

Product: N-ethylmaleimide reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MSSAKLFTPLKVGAITATNRVFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEATQISAQAKGYAGAPGLHSDEQ
IAAWKKITQGVHAQGGHMAVQLWHTGRISHASLQPGGQAPVAPSAINAGTRTSLRDENGQAIRVETSTPRALDTHEIPGI
VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDRYGGSVENRARLVLEVVDAGIKEWGADRIGIRLSPVGT
FQNVDNGPNEEADALYLIEALGKRGIAYLHMSEPDWAGGEPYSDAFREKVRARFHGPIIGAGAYTPEKAEDLIEKGLIDA
VAFGRDYIANPDLVARLQRKAELNPQRPESFYGGGAEGYTDYPTL

Sequences:

>Translated_365_residues
MSSAKLFTPLKVGAITATNRVFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEATQISAQAKGYAGAPGLHSDEQ
IAAWKKITQGVHAQGGHMAVQLWHTGRISHASLQPGGQAPVAPSAINAGTRTSLRDENGQAIRVETSTPRALDTHEIPGI
VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDRYGGSVENRARLVLEVVDAGIKEWGADRIGIRLSPVGT
FQNVDNGPNEEADALYLIEALGKRGIAYLHMSEPDWAGGEPYSDAFREKVRARFHGPIIGAGAYTPEKAEDLIEKGLIDA
VAFGRDYIANPDLVARLQRKAELNPQRPESFYGGGAEGYTDYPTL
>Mature_364_residues
SSAKLFTPLKVGAITATNRVFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEATQISAQAKGYAGAPGLHSDEQI
AAWKKITQGVHAQGGHMAVQLWHTGRISHASLQPGGQAPVAPSAINAGTRTSLRDENGQAIRVETSTPRALDTHEIPGIV
NDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDRYGGSVENRARLVLEVVDAGIKEWGADRIGIRLSPVGTF
QNVDNGPNEEADALYLIEALGKRGIAYLHMSEPDWAGGEPYSDAFREKVRARFHGPIIGAGAYTPEKAEDLIEKGLIDAV
AFGRDYIANPDLVARLQRKAELNPQRPESFYGGGAEGYTDYPTL

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=365, Percent_Identity=92.3287671232877, Blast_Score=692, Evalue=0.0,
Organism=Escherichia coli, GI1789463, Length=359, Percent_Identity=32.3119777158774, Blast_Score=129, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17559802, Length=353, Percent_Identity=28.0453257790368, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17565138, Length=351, Percent_Identity=28.7749287749288, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17540738, Length=369, Percent_Identity=28.9972899728997, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI72001454, Length=352, Percent_Identity=28.6931818181818, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17564188, Length=355, Percent_Identity=27.0422535211268, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17559804, Length=273, Percent_Identity=29.6703296703297, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17566914, Length=270, Percent_Identity=27.7777777777778, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=382, Percent_Identity=39.7905759162304, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6321973, Length=382, Percent_Identity=37.434554973822, Blast_Score=221, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39510; Mature: 39379

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAKLFTPLKVGAITATNRVFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEAT
CCCCCCCCCEEECEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEHHH
QISAQAKGYAGAPGLHSDEQIAAWKKITQGVHAQGGHMAVQLWHTGRISHASLQPGGQAP
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCC
VAPSAINAGTRTSLRDENGQAIRVETSTPRALDTHEIPGIVNDFRQAIANAREAGFDLVE
CCCHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEE
LHSAHGYLLHQFLSPSSNHRTDRYGGSVENRARLVLEVVDAGIKEWGADRIGIRLSPVGT
HHCCCHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
FQNVDNGPNEEADALYLIEALGKRGIAYLHMSEPDWAGGEPYSDAFREKVRARFHGPIIG
CCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEE
AGAYTPEKAEDLIEKGLIDAVAFGRDYIANPDLVARLQRKAELNPQRPESFYGGGAEGYT
CCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHCCCCCCCHHHCCCCCCCCC
DYPTL
CCCCC
>Mature Secondary Structure 
SSAKLFTPLKVGAITATNRVFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEAT
CCCCCCCCEEECEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEHHH
QISAQAKGYAGAPGLHSDEQIAAWKKITQGVHAQGGHMAVQLWHTGRISHASLQPGGQAP
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCC
VAPSAINAGTRTSLRDENGQAIRVETSTPRALDTHEIPGIVNDFRQAIANAREAGFDLVE
CCCHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEE
LHSAHGYLLHQFLSPSSNHRTDRYGGSVENRARLVLEVVDAGIKEWGADRIGIRLSPVGT
HHCCCHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
FQNVDNGPNEEADALYLIEALGKRGIAYLHMSEPDWAGGEPYSDAFREKVRARFHGPIIG
CCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEE
AGAYTPEKAEDLIEKGLIDAVAFGRDYIANPDLVARLQRKAELNPQRPESFYGGGAEGYT
CCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHCCCCCCCHHHCCCCCCCCC
DYPTL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]