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The map label for this gene is btuE [H]

Identifier: 29141686

GI number: 29141686

Start: 1289086

End: 1289637

Strand: Direct

Name: btuE [H]

Synonym: t1222

Alternate gene names: 29141686

Gene position: 1289086-1289637 (Clockwise)

Preceding gene: 29141685

Following gene: 29141687

Centisome position: 26.9

GC content: 49.64

Gene sequence:

>552_bases
ATGCAAAACTCGCTTCTTAATACTCACGTTACGACGATTGATGGAGAGGTCACTACACTTGAAAAATACGCCGGAAAAGT
GCTGCTCATTGTAAACGTCGCCTCCAGATGTGGCCTGACGCCGCAATATGAACAGTTGGAAAATATCCAGAAGGCGTGGG
CAGATCAGGGGTTTGTCGTGTTGGGTTTTCCCTGCAACCAGTTTATGGGGCAGGAGCCAGGCAGCGAAGAGGAGATTAAA
ACCTATTGTGCCAGCACCTGGGGCGTCACGTTCCCGATGTTCAGTAAAATTGATGTGAATGGCGACGCGCGCCATCCGCT
GTATCAAAAACTGATAGCCGCTGCGCCAACGGCAGTCGCGCCGGAGAAGAGCGGCTTTTACGAACGGATGGTAAGCAAAG
GGCGTACGCCACTCTATCCGGATGATATTCTGTGGAATTTTGAAAAATTTTTGGTAGGCCGGGATGGTCAGGTGGTACAG
CGTTTTTCGCCGGATATGACGCCTGAAGATCCTATTGTGATGGAAAGCATAAAAATCGCGTTGGCAAAATAA

Upstream 100 bases:

>100_bases
GCGCGTTAGCGCATCGGTAAACAACTGCTTTCATCATTCGCAGTCTATGATTAAAGGCTAACGATGAAACCCGCTATTCT
ACCGACATCAGGGAGACACC

Downstream 100 bases:

>100_bases
TGTCTCAATTGATGCAATTAAAGGACGTCGCCGAGAGCACCCGCCTGGGGCCGCTTTCCGGCGAAGTAAGCGCAGGAGAG
ATCCTTCATCTTGTCGGGCC

Product: glutathione peroxidase

Products: ADP; phosphate; cob(I)alamin [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MQNSLLNTHVTTIDGEVTTLEKYAGKVLLIVNVASRCGLTPQYEQLENIQKAWADQGFVVLGFPCNQFMGQEPGSEEEIK
TYCASTWGVTFPMFSKIDVNGDARHPLYQKLIAAAPTAVAPEKSGFYERMVSKGRTPLYPDDILWNFEKFLVGRDGQVVQ
RFSPDMTPEDPIVMESIKIALAK

Sequences:

>Translated_183_residues
MQNSLLNTHVTTIDGEVTTLEKYAGKVLLIVNVASRCGLTPQYEQLENIQKAWADQGFVVLGFPCNQFMGQEPGSEEEIK
TYCASTWGVTFPMFSKIDVNGDARHPLYQKLIAAAPTAVAPEKSGFYERMVSKGRTPLYPDDILWNFEKFLVGRDGQVVQ
RFSPDMTPEDPIVMESIKIALAK
>Mature_183_residues
MQNSLLNTHVTTIDGEVTTLEKYAGKVLLIVNVASRCGLTPQYEQLENIQKAWADQGFVVLGFPCNQFMGQEPGSEEEIK
TYCASTWGVTFPMFSKIDVNGDARHPLYQKLIAAAPTAVAPEKSGFYERMVSKGRTPLYPDDILWNFEKFLVGRDGQVVQ
RFSPDMTPEDPIVMESIKIALAK

Specific function: Not essential for B12 transport; however, it is an auxiliary component of the transport system [H]

COG id: COG0386

COG function: function code O; Glutathione peroxidase

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutathione peroxidase family [H]

Homologues:

Organism=Homo sapiens, GI75709200, Length=164, Percent_Identity=42.0731707317073, Blast_Score=118, Evalue=3e-27,
Organism=Homo sapiens, GI90903240, Length=173, Percent_Identity=39.8843930635838, Blast_Score=117, Evalue=6e-27,
Organism=Homo sapiens, GI15618997, Length=160, Percent_Identity=40, Blast_Score=115, Evalue=2e-26,
Organism=Homo sapiens, GI6006001, Length=162, Percent_Identity=43.2098765432099, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI4557629, Length=157, Percent_Identity=41.4012738853503, Blast_Score=104, Evalue=4e-23,
Organism=Homo sapiens, GI192455698, Length=171, Percent_Identity=36.2573099415205, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI90903238, Length=139, Percent_Identity=41.726618705036, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI41406084, Length=155, Percent_Identity=41.2903225806452, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI33186887, Length=154, Percent_Identity=38.961038961039, Blast_Score=95, Evalue=4e-20,
Organism=Homo sapiens, GI32967607, Length=194, Percent_Identity=32.4742268041237, Blast_Score=83, Evalue=1e-16,
Organism=Escherichia coli, GI1788003, Length=183, Percent_Identity=85.792349726776, Blast_Score=338, Evalue=9e-95,
Organism=Caenorhabditis elegans, GI17506887, Length=181, Percent_Identity=41.4364640883978, Blast_Score=132, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17535473, Length=168, Percent_Identity=44.6428571428571, Blast_Score=130, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17550320, Length=187, Percent_Identity=37.9679144385027, Blast_Score=122, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI193209493, Length=187, Percent_Identity=35.2941176470588, Blast_Score=116, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI71993584, Length=160, Percent_Identity=37.5, Blast_Score=110, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI193210707, Length=160, Percent_Identity=36.25, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71993573, Length=95, Percent_Identity=36.8421052631579, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71999642, Length=76, Percent_Identity=42.1052631578947, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6319721, Length=168, Percent_Identity=45.8333333333333, Blast_Score=145, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6322228, Length=154, Percent_Identity=46.1038961038961, Blast_Score=138, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6322826, Length=153, Percent_Identity=40.5228758169935, Blast_Score=112, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24656772, Length=163, Percent_Identity=36.1963190184049, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24656777, Length=163, Percent_Identity=36.1963190184049, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24656767, Length=169, Percent_Identity=35.5029585798817, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI85726443, Length=168, Percent_Identity=35.7142857142857, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000889
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00255 GSHPx [H]

EC number: NA

Molecular weight: Translated: 20424; Mature: 20424

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00460 GLUTATHIONE_PEROXID_1 ; PS00763 GLUTATHIONE_PEROXID_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNSLLNTHVTTIDGEVTTLEKYAGKVLLIVNVASRCGLTPQYEQLENIQKAWADQGFVV
CCCCCCCCEEEEECCCHHHHHHHCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHCCCCEEE
LGFPCNQFMGQEPGSEEEIKTYCASTWGVTFPMFSKIDVNGDARHPLYQKLIAAAPTAVA
ECCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCC
PEKSGFYERMVSKGRTPLYPDDILWNFEKFLVGRDGQVVQRFSPDMTPEDPIVMESIKIA
CCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHE
LAK
ECC
>Mature Secondary Structure
MQNSLLNTHVTTIDGEVTTLEKYAGKVLLIVNVASRCGLTPQYEQLENIQKAWADQGFVV
CCCCCCCCEEEEECCCHHHHHHHCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHCCCCEEE
LGFPCNQFMGQEPGSEEEIKTYCASTWGVTFPMFSKIDVNGDARHPLYQKLIAAAPTAVA
ECCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCC
PEKSGFYERMVSKGRTPLYPDDILWNFEKFLVGRDGQVVQRFSPDMTPEDPIVMESIKIA
CCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCHHHHHHHHE
LAK
ECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; cob(I)alamin [Periplasm]; H2O [C]

Specific reaction: ATP + cob(I)alamin [Periplasm] + H2O = ADP + phosphate + cob(I)alamin [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3528129; 9097039; 9278503 [H]