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The map label for this gene is dadX

Identifier: 29141550

GI number: 29141550

Start: 1156855

End: 1157925

Strand: Direct

Name: dadX

Synonym: t1075

Alternate gene names: 29141550

Gene position: 1156855-1157925 (Clockwise)

Preceding gene: 29141549

Following gene: 29141553

Centisome position: 24.14

GC content: 57.8

Gene sequence:

>1071_bases
ATGACCCGCCCTATACAGGCCAGCCTTGATTTACAGGTAATGAAACAAAATTTGGCTATTGTGCGCCGGGCGGCCCCGGA
GGCGCGCGTCTGGTCGGTAGTGAAAGCCAACGCCTACGGCCACGGTATTGAACGCGTCTGGAGCGCGCTGGGCGCCACAG
ATGGTTTCGCTATGCTCAACCTTGAAGAGGCGATTACCCTGCGTGAGCGCGGGTGGAAAGGGCCGATATTGATGCTGGAG
GGGTTTTTCCATGCGCAAGACCTGGAGGCGTATGACACTTATCGGCTGACCACCTGCATCCACAGTAACTGGCAGTTGAA
AGCGCTGCAAAATGCGCGGCTTAACGCGCCGCTGGATATCTATGTCAAAGTCAACAGCGGTATGAACCGACTCGGTTTTC
AGCCTGAGCGGGCGCAGACCGTCTGGCAGCAATTGCGGGCAATGCGCAATGTCGGTGAAATGACCCTGATGTCACATTTT
GCTCAGGCCGATCATCCGGAAGGTATCGGGGAGGCGATGAGACGTATTGCGCTGGCGACAGAAGGCCTGCAGTGTGCATA
CTCGTTATCAAATTCGGCAGCGACGCTGTGGCATCCCCAGGCGCATTATGACTGGGTCAGGCCGGGCATTATTTTGTACG
GCGCGTCGCCGTCAGGACAGTGGCGGGATATTGCCGATACCGGACTAAAACCCGTGATGACGCTGAGTAGCGAAATTATT
GGCGTGCAGACGCTGAGCGCGGGTGAAAGAGTAGGCTATGGCGGAGGGTATTCTGTGACTCAGGAACAACGCATTGGCAT
CGTCGCGGCGGGTTATGCCGATGGCTATCCACGCCATGCGCCGACCGGGACGCCCGTGCTGGTGGACGGTATCCGTACCA
GAACAGTAGGCACCGTTTCAATGGATATGCTGGCGGTGGATTTGACGCCGTGTCCGCAGGCGGGAATCGGCACGCCGGTT
GAATTATGGGGCAAAGAAATTAAGGTCGATGATGTCGCCTCTGCGGCAGGCACGCTGGGCTATGGGCTACTGTGCGCCGT
AGCGCCGCGCGTGCCGTTTGTGACAACGTAA

Upstream 100 bases:

>100_bases
ATACCCTACGACGATCTGAGCGTGGCGCGATACCGTTCCGATTTTACGCCCACCCGCCCGCAACGTTTGCATAGCGCGCA
TAACTGATAAAGGAAGTGAA

Downstream 100 bases:

>100_bases
CCTAACTCAGGCTAAGGCGTCGACCGGTCCACCGGGGGCGGGTCGACGTTGTCCGGCGACCTTATTCTGCTTCGTCTTCG
GCGACGCGTACCCCAATCTT

Product: alanine racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MTRPIQASLDLQVMKQNLAIVRRAAPEARVWSVVKANAYGHGIERVWSALGATDGFAMLNLEEAITLRERGWKGPILMLE
GFFHAQDLEAYDTYRLTTCIHSNWQLKALQNARLNAPLDIYVKVNSGMNRLGFQPERAQTVWQQLRAMRNVGEMTLMSHF
AQADHPEGIGEAMRRIALATEGLQCAYSLSNSAATLWHPQAHYDWVRPGIILYGASPSGQWRDIADTGLKPVMTLSSEII
GVQTLSAGERVGYGGGYSVTQEQRIGIVAAGYADGYPRHAPTGTPVLVDGIRTRTVGTVSMDMLAVDLTPCPQAGIGTPV
ELWGKEIKVDDVASAAGTLGYGLLCAVAPRVPFVTT

Sequences:

>Translated_356_residues
MTRPIQASLDLQVMKQNLAIVRRAAPEARVWSVVKANAYGHGIERVWSALGATDGFAMLNLEEAITLRERGWKGPILMLE
GFFHAQDLEAYDTYRLTTCIHSNWQLKALQNARLNAPLDIYVKVNSGMNRLGFQPERAQTVWQQLRAMRNVGEMTLMSHF
AQADHPEGIGEAMRRIALATEGLQCAYSLSNSAATLWHPQAHYDWVRPGIILYGASPSGQWRDIADTGLKPVMTLSSEII
GVQTLSAGERVGYGGGYSVTQEQRIGIVAAGYADGYPRHAPTGTPVLVDGIRTRTVGTVSMDMLAVDLTPCPQAGIGTPV
ELWGKEIKVDDVASAAGTLGYGLLCAVAPRVPFVTT
>Mature_355_residues
TRPIQASLDLQVMKQNLAIVRRAAPEARVWSVVKANAYGHGIERVWSALGATDGFAMLNLEEAITLRERGWKGPILMLEG
FFHAQDLEAYDTYRLTTCIHSNWQLKALQNARLNAPLDIYVKVNSGMNRLGFQPERAQTVWQQLRAMRNVGEMTLMSHFA
QADHPEGIGEAMRRIALATEGLQCAYSLSNSAATLWHPQAHYDWVRPGIILYGASPSGQWRDIADTGLKPVMTLSSEIIG
VQTLSAGERVGYGGGYSVTQEQRIGIVAAGYADGYPRHAPTGTPVLVDGIRTRTVGTVSMDMLAVDLTPCPQAGIGTPVE
LWGKEIKVDDVASAAGTLGYGLLCAVAPRVPFVTT

Specific function: Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family

Homologues:

Organism=Escherichia coli, GI1787439, Length=355, Percent_Identity=85.6338028169014, Blast_Score=639, Evalue=0.0,
Organism=Escherichia coli, GI1790487, Length=347, Percent_Identity=42.3631123919308, Blast_Score=280, Evalue=1e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ALR2_SALTI (Q8Z688)

Other databases:

- EMBL:   AL627272
- EMBL:   AE014613
- RefSeq:   NP_456309.1
- RefSeq:   NP_804892.1
- ProteinModelPortal:   Q8Z688
- SMR:   Q8Z688
- GeneID:   1070423
- GeneID:   1248285
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t1075
- KEGG:   sty:STY1930
- HOGENOM:   HBG712172
- OMA:   SVELWGD
- ProtClustDB:   PRK03646
- BioCyc:   SENT209261:T1075-MONOMER
- BioCyc:   SENT220341:STY1930-MONOMER
- BRENDA:   5.1.1.1
- HAMAP:   MF_01201
- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622
- Gene3D:   G3DSA:2.40.37.10
- PRINTS:   PR00992
- TIGRFAMs:   TIGR00492

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N; SSF50621 Racem_decarbox_C

EC number: =5.1.1.1

Molecular weight: Translated: 38733; Mature: 38602

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: ACT_SITE 35-35 ACT_SITE 253-253

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPIQASLDLQVMKQNLAIVRRAAPEARVWSVVKANAYGHGIERVWSALGATDGFAMLN
CCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHEEECCCCCCCHHHHHHHHCCCCCEEEEE
LEEAITLRERGWKGPILMLEGFFHAQDLEAYDTYRLTTCIHSNWQLKALQNARLNAPLDI
HHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHCEEEEEEECCCEEEEEECCCCCCCCEEE
YVKVNSGMNRLGFQPERAQTVWQQLRAMRNVGEMTLMSHFAQADHPEGIGEAMRRIALAT
EEEECCCCHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EGLQCAYSLSNSAATLWHPQAHYDWVRPGIILYGASPSGQWRDIADTGLKPVMTLSSEII
CCCCEEEECCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCHHHCCCCHHHHHHHHHH
GVQTLSAGERVGYGGGYSVTQEQRIGIVAAGYADGYPRHAPTGTPVLVDGIRTRTVGTVS
EEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCEEEEEEEEE
MDMLAVDLTPCPQAGIGTPVELWGKEIKVDDVASAAGTLGYGLLCAVAPRVPFVTT
EEEEEEECCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TRPIQASLDLQVMKQNLAIVRRAAPEARVWSVVKANAYGHGIERVWSALGATDGFAMLN
CCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHEEECCCCCCCHHHHHHHHCCCCCEEEEE
LEEAITLRERGWKGPILMLEGFFHAQDLEAYDTYRLTTCIHSNWQLKALQNARLNAPLDI
HHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHCEEEEEEECCCEEEEEECCCCCCCCEEE
YVKVNSGMNRLGFQPERAQTVWQQLRAMRNVGEMTLMSHFAQADHPEGIGEAMRRIALAT
EEEECCCCHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EGLQCAYSLSNSAATLWHPQAHYDWVRPGIILYGASPSGQWRDIADTGLKPVMTLSSEII
CCCCEEEECCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCHHHCCCCHHHHHHHHHH
GVQTLSAGERVGYGGGYSVTQEQRIGIVAAGYADGYPRHAPTGTPVLVDGIRTRTVGTVS
EEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEECCEEEEEEEEE
MDMLAVDLTPCPQAGIGTPVELWGKEIKVDDVASAAGTLGYGLLCAVAPRVPFVTT
EEEEEEECCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504