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The map label for this gene is yebU [H]

Identifier: 29141504

GI number: 29141504

Start: 1109520

End: 1110959

Strand: Reverse

Name: yebU [H]

Synonym: t1028

Alternate gene names: 29141504

Gene position: 1110959-1109520 (Counterclockwise)

Preceding gene: 29141505

Following gene: 29141503

Centisome position: 23.18

GC content: 55.07

Gene sequence:

>1440_bases
GTGGCTCAACACGCTGTCTATTTTCCTGACGCTTTTCTGACGCAAATGCGTGAAGCAATGCCTTCCACGCTCTCTTTTGA
TGAATTTATTTCGGCCTGCCAACGCCCGTTGCGCCGCAGTATACGCATCAATACGCTTAAAATATCCGTGGCTGATTTCC
TTGCCCTAATCGCGCCCTACGGCTGGTCGCTCACGCCAATTCCGTGGTGTCATGAAGGATTCTGGATCGAGCGCGATGAT
GAAGAGGCATTGCCGCTTGGCAGTACCGCTGAGCATTTAAGCGGCCTGTTTTACATTCAGGAGGCCAGTTCGATGCTGCC
GGTCGCGGCCCTGTTCGCCGATGATAATCATCCGCAGCGGGTCATGGATATGGCAGCGGCGCCTGGCTCCAAAACCACGC
AGATCGCTGCCCGCATGGGGAACCGCGGCGCCATTCTGGCCAACGAATTTTCGGCCAGCCGCGTCAAAGTTCTGCACGCT
AATATCAGCCGATGTGGAATCGCCAATACCGCACTGACGCATTTCGATGGCCGCGTATTTGGCGCTGCGTTACCGGAGAT
GTTTGACGCTATCCTGCTGGATGCGCCCTGCTCTGGCGAAGGCGTAGTACGTAAAGATCCTGATGCGCTAAAAAACTGGT
CGCCAGAAAGTAATCTGGATATCGCGGCGACACAGCGGGAACTGCTCGACAGCGCTTTTCATGCTTTACGACCCGGCGGC
ACGCTGGTCTACTCCACCTGCACGTTAAATCGCCAGGAAAACGAAGCGGTCTGCCTCTGGCTGAAAGAGACCTACGCCGA
CGCCGTTGAATTTTTGCCGCTGGGTGATCTGTTTCCTGACGCTGACCGCGCCCTCACCCCGGAAGGCTTCCTGCACGTCT
TCCCGCAAATTTACGACTGCGAAGGATTCTTCGTCGCACGTCTGCGTAAAATGTCATCCCTTCCAGCCATGCCCGCGCCG
GGGTATAAAGTGGGGGCGTTTCCTTTTACCTCCCTCAAAGGCCGCGAAGCATTAAACGTTACCCAGGCGGCCAACGCCGT
CGGACTGCTATGGGATGAAAATCTCCATCTTTGGCAGCGTGAAAAAGAGGTCTGGCTGTTCCCGGCAGAGATTGAATCAC
TGATCGGAAAAGTCCGTTTTTCCCGGCTCGGCATTAAGCTGGCCGAAAGTCATAATAAAGGCTATCGCTGGCAACATGAG
GCGACGATAGCCCTTGCCTGCCCGACTCATGCTCACGCGTTCGAACTTTCTGTGCAAGAGGCGGAGGAGTGGTATCGCGG
TCGGGATATTTATCCGCAAACACCCCCTGCCGCTGATGATGTCCTGGTGACGTTCCAGCATCAGCCACTTGGTCTGGCAA
AACGTATCGGCGCCAGAATAAAGAACAGTTACCCGCGTGAACTGGTGCGGGATGGAAAACTCTTTACTGGTAATAGTTAA

Upstream 100 bases:

>100_bases
GGGGTACCGCTCTGCCACGTTAAACACCAGGCTCCGGCGTACTCGCGCCGGAGCGTTTTATGCTACACTGCGCGCCTGTT
TTTTTGCCGGCGATACACCT

Downstream 100 bases:

>100_bases
AAGGCGCACAAAAAAACCGCACTTTTTGACTGGCGCTTTTGCGTACGTTGACTACGCTGAAAAATGGACGGCTAAACTGG
TCGTACCACAATTAGCGTAT

Product: rRNA (cytosine-C(5)-)-methyltransferase RsmF

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MAQHAVYFPDAFLTQMREAMPSTLSFDEFISACQRPLRRSIRINTLKISVADFLALIAPYGWSLTPIPWCHEGFWIERDD
EEALPLGSTAEHLSGLFYIQEASSMLPVAALFADDNHPQRVMDMAAAPGSKTTQIAARMGNRGAILANEFSASRVKVLHA
NISRCGIANTALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNLDIAATQRELLDSAFHALRPGG
TLVYSTCTLNRQENEAVCLWLKETYADAVEFLPLGDLFPDADRALTPEGFLHVFPQIYDCEGFFVARLRKMSSLPAMPAP
GYKVGAFPFTSLKGREALNVTQAANAVGLLWDENLHLWQREKEVWLFPAEIESLIGKVRFSRLGIKLAESHNKGYRWQHE
ATIALACPTHAHAFELSVQEAEEWYRGRDIYPQTPPAADDVLVTFQHQPLGLAKRIGARIKNSYPRELVRDGKLFTGNS

Sequences:

>Translated_479_residues
MAQHAVYFPDAFLTQMREAMPSTLSFDEFISACQRPLRRSIRINTLKISVADFLALIAPYGWSLTPIPWCHEGFWIERDD
EEALPLGSTAEHLSGLFYIQEASSMLPVAALFADDNHPQRVMDMAAAPGSKTTQIAARMGNRGAILANEFSASRVKVLHA
NISRCGIANTALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNLDIAATQRELLDSAFHALRPGG
TLVYSTCTLNRQENEAVCLWLKETYADAVEFLPLGDLFPDADRALTPEGFLHVFPQIYDCEGFFVARLRKMSSLPAMPAP
GYKVGAFPFTSLKGREALNVTQAANAVGLLWDENLHLWQREKEVWLFPAEIESLIGKVRFSRLGIKLAESHNKGYRWQHE
ATIALACPTHAHAFELSVQEAEEWYRGRDIYPQTPPAADDVLVTFQHQPLGLAKRIGARIKNSYPRELVRDGKLFTGNS
>Mature_478_residues
AQHAVYFPDAFLTQMREAMPSTLSFDEFISACQRPLRRSIRINTLKISVADFLALIAPYGWSLTPIPWCHEGFWIERDDE
EALPLGSTAEHLSGLFYIQEASSMLPVAALFADDNHPQRVMDMAAAPGSKTTQIAARMGNRGAILANEFSASRVKVLHAN
ISRCGIANTALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNLDIAATQRELLDSAFHALRPGGT
LVYSTCTLNRQENEAVCLWLKETYADAVEFLPLGDLFPDADRALTPEGFLHVFPQIYDCEGFFVARLRKMSSLPAMPAPG
YKVGAFPFTSLKGREALNVTQAANAVGLLWDENLHLWQREKEVWLFPAEIESLIGKVRFSRLGIKLAESHNKGYRWQHEA
TIALACPTHAHAFELSVQEAEEWYRGRDIYPQTPPAADDVLVTFQHQPLGLAKRIGARIKNSYPRELVRDGKLFTGNS

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=328, Percent_Identity=35.6707317073171, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI76150623, Length=328, Percent_Identity=35.6707317073171, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI11545785, Length=168, Percent_Identity=39.8809523809524, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI39995082, Length=287, Percent_Identity=28.5714285714286, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI301336155, Length=287, Percent_Identity=28.5714285714286, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI32698918, Length=167, Percent_Identity=35.9281437125749, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI40316918, Length=209, Percent_Identity=29.6650717703349, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI23199998, Length=305, Percent_Identity=28.8524590163934, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI8922322, Length=305, Percent_Identity=28.8524590163934, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI270288816, Length=305, Percent_Identity=28.8524590163934, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI270288818, Length=305, Percent_Identity=28.8524590163934, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI87081985, Length=479, Percent_Identity=85.1774530271399, Blast_Score=863, Evalue=0.0,
Organism=Escherichia coli, GI2367212, Length=305, Percent_Identity=28.8524590163934, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17536757, Length=322, Percent_Identity=33.2298136645963, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI25143471, Length=297, Percent_Identity=29.2929292929293, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324268, Length=306, Percent_Identity=37.2549019607843, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6319447, Length=314, Percent_Identity=27.3885350318471, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI22024126, Length=312, Percent_Identity=35.2564102564103, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21355201, Length=287, Percent_Identity=30.6620209059233, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24668781, Length=237, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21356513, Length=237, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 53258; Mature: 53127

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQHAVYFPDAFLTQMREAMPSTLSFDEFISACQRPLRRSIRINTLKISVADFLALIAPY
CCCCEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEEEEEEEHHHHHHHHHCC
GWSLTPIPWCHEGFWIERDDEEALPLGSTAEHLSGLFYIQEASSMLPVAALFADDNHPQR
CCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHEEEEECCCCCCCEEEEECCCCCHHH
VMDMAAAPGSKTTQIAARMGNRGAILANEFSASRVKVLHANISRCGIANTALTHFDGRVF
HHHHHCCCCCCHHHHHHHCCCCCEEEEECCCCCEEEEEECCHHHCCCCHHHHHHCCCHHH
GAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNLDIAATQRELLDSAFHALRPGG
HHHHHHHHHHHEECCCCCCCCCEECCCHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCC
TLVYSTCTLNRQENEAVCLWLKETYADAVEFLPLGDLFPDADRALTPEGFLHVFPQIYDC
EEEEEEEECCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCHHCCC
EGFFVARLRKMSSLPAMPAPGYKVGAFPFTSLKGREALNVTQAANAVGLLWDENLHLWQR
CHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHCCEEEEEEECCCHHEEC
EKEVWLFPAEIESLIGKVRFSRLGIKLAESHNKGYRWQHEATIALACPTHAHAFELSVQE
CCCEEEEHHHHHHHHHHHHHHHHCEEEECCCCCCCEEEECCEEEEECCCCCCEEEEEHHH
AEEWYRGRDIYPQTPPAADDVLVTFQHQPLGLAKRIGARIKNSYPRELVRDGKLFTGNS
HHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCEEECCC
>Mature Secondary Structure 
AQHAVYFPDAFLTQMREAMPSTLSFDEFISACQRPLRRSIRINTLKISVADFLALIAPY
CCCEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEEEEEEEHHHHHHHHHCC
GWSLTPIPWCHEGFWIERDDEEALPLGSTAEHLSGLFYIQEASSMLPVAALFADDNHPQR
CCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHEEEEECCCCCCCEEEEECCCCCHHH
VMDMAAAPGSKTTQIAARMGNRGAILANEFSASRVKVLHANISRCGIANTALTHFDGRVF
HHHHHCCCCCCHHHHHHHCCCCCEEEEECCCCCEEEEEECCHHHCCCCHHHHHHCCCHHH
GAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNLDIAATQRELLDSAFHALRPGG
HHHHHHHHHHHEECCCCCCCCCEECCCHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCC
TLVYSTCTLNRQENEAVCLWLKETYADAVEFLPLGDLFPDADRALTPEGFLHVFPQIYDC
EEEEEEEECCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCHHCCC
EGFFVARLRKMSSLPAMPAPGYKVGAFPFTSLKGREALNVTQAANAVGLLWDENLHLWQR
CHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHCCEEEEEEECCCHHEEC
EKEVWLFPAEIESLIGKVRFSRLGIKLAESHNKGYRWQHEATIALACPTHAHAFELSVQE
CCCEEEEHHHHHHHHHHHHHHHHCEEEECCCCCCCEEEECCEEEEECCCCCCEEEEEHHH
AEEWYRGRDIYPQTPPAADDVLVTFQHQPLGLAKRIGARIKNSYPRELVRDGKLFTGNS
HHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA