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The map label for this gene is amyA [H]

Identifier: 29141401

GI number: 29141401

Start: 1009782

End: 1011266

Strand: Reverse

Name: amyA [H]

Synonym: t0914

Alternate gene names: 29141401

Gene position: 1011266-1009782 (Counterclockwise)

Preceding gene: 29141402

Following gene: 29141399

Centisome position: 21.1

GC content: 51.31

Gene sequence:

>1485_bases
ATGAAAAACCCCACGTTATTGCAGTACTTCCACTGGTATTATCCCGACGGAGGAAAACTCTGGTCTGAGCTGGCGGAACG
TGCTGATGGGCTGAATGATATCGGTATCAATATGGTCTGGCTACCGCCCGCCTGTAAAGGCGCCTCCGGCGGCTATTCCG
TAGGCTATGATAGCTACGACCTGTTTGACCTCGGCGAATTTGACCAAAAAGGAACTATCGCGACAAAGTACGGCGATAAA
CGCCAGTTACTGACGGCGATAGACGCGCTCAAAAAAAATAATATTGCGGTGCTGCTCGACGTCGTCGTGAACCACAAAAT
GGGCGCAGACGAAAAAGAACGTATTCGCGTTCAGCGCGTGAATCAGGATGACCGCACGCAAATTGATGACAACATCATTG
AATGCGAAGGCTGGACGCGCTACACCTTCCCTGCCCGCGCGGGCCAGTATTCCAACTTTATTTGGGACTATCACTGTTTC
AGCGGCATTGATCACATCGAGAATCCCGACGAAGACGGCATTTTTAAGATCGTCAATGACTATACCGGCGATGGCTGGAA
CGATCAGGTTGATGATGAGCTGGGTAATTTCGACTATCTGATGGGGGAAAATATCGATTTTCGCAATCACGCGGTTACGG
AAGAGATTAAATATTGGGCTCGTTGGGTCATGGAACAAACCCATTGTGACGGCTTTCGCCTGGACGCGGTAAAACATATA
CCCGCCTGGTTTTATAAAGAATGGATTGAGCATGTACAGGCGGTTGCGCCAAAACCGCTGTTTATTGTCGCAGAATACTG
GTCGCATGAAGTGGATAAACTGCAAACGTACATCGATCAGGCCGACGGGAAAACCATGCTGTTCGACGCGCCGTTGCAGA
TGAAATTTCATGAAGCCTCGCGCCAGGGCGCGGAGTATGACATGCGCCACATTTTCACCGGCACGCTGGTAGAAGCCGAC
CCTTTTCATGCGGTGACGCTGGTCGCTAACCACGATACACAACTGTTACAGGCGCTGGAAGCGCCGGTAGAACCCTGGTT
CAAACCATTGGCCTATGCGCTGATCCTGCTTCGTGAAAACGGCGTACCGTCGGTGTTTTATCCCGATTTATACGGCGCCA
GCTATGAGGATAACGGCGAAAATGGCGAGACCTGTCGGGTCGACATGCCGGTGATTAACCAACTGGATCGGCTGATCCTC
GCTCGTCAGCGTTTTGCGCACGGTATACAAACACTCTTTTTCGATCATCCCAACTGTATCGCCTTTAGTCGCAGCGGTAC
TGAAGAGAATCCAGGCTGTGTGGTCGTACTTTCCAATGGCGACGACGGTGAAAAAACCCTCCTGCTCGGCGACAATTACG
CTAACAAGACCTGGCGTGATTTTCTGGGAAACCGCAGTGAGCATGTTGTAACTAATGATCAAGGCGAAGCGACGTTCTTC
TGCAACGCAGGCAGCGTCAGCGTGTGGGTCATTGAGGACGTGTGA

Upstream 100 bases:

>100_bases
TCGTGCCAGACGCACCTGGCGCCTCATAATATTTTCCCGTCTCGTATGAAAATTCTTCCATACTCCAGAGGTCGGCTAAA
CGACTTCTGGAGCACGGAAG

Downstream 100 bases:

>100_bases
TGAGCGTTCCCGGCGGCAGAGCGCTGCCGGGAAGCGAAATTACGGCAGCGGCGTCGGCAGCGGCGTTTTTTCTAACGCGG
CGGCGCACTCCGGCGTCGGA

Product: cytoplasmic alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MKNPTLLQYFHWYYPDGGKLWSELAERADGLNDIGINMVWLPPACKGASGGYSVGYDSYDLFDLGEFDQKGTIATKYGDK
RQLLTAIDALKKNNIAVLLDVVVNHKMGADEKERIRVQRVNQDDRTQIDDNIIECEGWTRYTFPARAGQYSNFIWDYHCF
SGIDHIENPDEDGIFKIVNDYTGDGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKYWARWVMEQTHCDGFRLDAVKHI
PAWFYKEWIEHVQAVAPKPLFIVAEYWSHEVDKLQTYIDQADGKTMLFDAPLQMKFHEASRQGAEYDMRHIFTGTLVEAD
PFHAVTLVANHDTQLLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGASYEDNGENGETCRVDMPVINQLDRLIL
ARQRFAHGIQTLFFDHPNCIAFSRSGTEENPGCVVVLSNGDDGEKTLLLGDNYANKTWRDFLGNRSEHVVTNDQGEATFF
CNAGSVSVWVIEDV

Sequences:

>Translated_494_residues
MKNPTLLQYFHWYYPDGGKLWSELAERADGLNDIGINMVWLPPACKGASGGYSVGYDSYDLFDLGEFDQKGTIATKYGDK
RQLLTAIDALKKNNIAVLLDVVVNHKMGADEKERIRVQRVNQDDRTQIDDNIIECEGWTRYTFPARAGQYSNFIWDYHCF
SGIDHIENPDEDGIFKIVNDYTGDGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKYWARWVMEQTHCDGFRLDAVKHI
PAWFYKEWIEHVQAVAPKPLFIVAEYWSHEVDKLQTYIDQADGKTMLFDAPLQMKFHEASRQGAEYDMRHIFTGTLVEAD
PFHAVTLVANHDTQLLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGASYEDNGENGETCRVDMPVINQLDRLIL
ARQRFAHGIQTLFFDHPNCIAFSRSGTEENPGCVVVLSNGDDGEKTLLLGDNYANKTWRDFLGNRSEHVVTNDQGEATFF
CNAGSVSVWVIEDV
>Mature_494_residues
MKNPTLLQYFHWYYPDGGKLWSELAERADGLNDIGINMVWLPPACKGASGGYSVGYDSYDLFDLGEFDQKGTIATKYGDK
RQLLTAIDALKKNNIAVLLDVVVNHKMGADEKERIRVQRVNQDDRTQIDDNIIECEGWTRYTFPARAGQYSNFIWDYHCF
SGIDHIENPDEDGIFKIVNDYTGDGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKYWARWVMEQTHCDGFRLDAVKHI
PAWFYKEWIEHVQAVAPKPLFIVAEYWSHEVDKLQTYIDQADGKTMLFDAPLQMKFHEASRQGAEYDMRHIFTGTLVEAD
PFHAVTLVANHDTQLLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGASYEDNGENGETCRVDMPVINQLDRLIL
ARQRFAHGIQTLFFDHPNCIAFSRSGTEENPGCVVVLSNGDDGEKTLLLGDNYANKTWRDFLGNRSEHVVTNDQGEATFF
CNAGSVSVWVIEDV

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1788236, Length=494, Percent_Identity=88.663967611336, Blast_Score=920, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013776
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 56451; Mature: 56451

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNPTLLQYFHWYYPDGGKLWSELAERADGLNDIGINMVWLPPACKGASGGYSVGYDSYD
CCCCCEEEEEEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
LFDLGEFDQKGTIATKYGDKRQLLTAIDALKKNNIAVLLDVVVNHKMGADEKERIRVQRV
CCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEEEEHHCCCCCCCHHHCEEEEEC
NQDDRTQIDDNIIECEGWTRYTFPARAGQYSNFIWDYHCFSGIDHIENPDEDGIFKIVND
CCCCCCCCCCCEEEECCCEEEECCCCCCCCCCEEEEEHHHCCCCCCCCCCCCCEEEEEEC
YTGDGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKYWARWVMEQTHCDGFRLDAVKHI
CCCCCCCCCHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH
PAWFYKEWIEHVQAVAPKPLFIVAEYWSHEVDKLQTYIDQADGKTMLFDAPLQMKFHEAS
HHHHHHHHHHHHHHHCCCCEEEEEHHHCHHHHHHHHHHHHCCCCEEEEECCCCEEEHHHH
RQGAEYDMRHIFTGTLVEADPFHAVTLVANHDTQLLQALEAPVEPWFKPLAYALILLREN
CCCCCCCHHHHEECEEEECCCCEEEEEEECCCHHHHHHHHCCHHHHHHHHHHHEEEEECC
GVPSVFYPDLYGASYEDNGENGETCRVDMPVINQLDRLILARQRFAHGIQTLFFDHPNCI
CCCCEECCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHEECCCCEE
AFSRSGTEENPGCVVVLSNGDDGEKTLLLGDNYANKTWRDFLGNRSEHVVTNDQGEATFF
EEECCCCCCCCCEEEEEECCCCCCEEEEEECCCCCHHHHHHHCCCCCEEEECCCCCEEEE
CNAGSVSVWVIEDV
EECCCEEEEEEECC
>Mature Secondary Structure
MKNPTLLQYFHWYYPDGGKLWSELAERADGLNDIGINMVWLPPACKGASGGYSVGYDSYD
CCCCCEEEEEEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
LFDLGEFDQKGTIATKYGDKRQLLTAIDALKKNNIAVLLDVVVNHKMGADEKERIRVQRV
CCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEEEEHHCCCCCCCHHHCEEEEEC
NQDDRTQIDDNIIECEGWTRYTFPARAGQYSNFIWDYHCFSGIDHIENPDEDGIFKIVND
CCCCCCCCCCCEEEECCCEEEECCCCCCCCCCEEEEEHHHCCCCCCCCCCCCCEEEEEEC
YTGDGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKYWARWVMEQTHCDGFRLDAVKHI
CCCCCCCCCHHHHHCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH
PAWFYKEWIEHVQAVAPKPLFIVAEYWSHEVDKLQTYIDQADGKTMLFDAPLQMKFHEAS
HHHHHHHHHHHHHHHCCCCEEEEEHHHCHHHHHHHHHHHHCCCCEEEEECCCCEEEHHHH
RQGAEYDMRHIFTGTLVEADPFHAVTLVANHDTQLLQALEAPVEPWFKPLAYALILLREN
CCCCCCCHHHHEECEEEECCCCEEEEEEECCCHHHHHHHHCCHHHHHHHHHHHEEEEECC
GVPSVFYPDLYGASYEDNGENGETCRVDMPVINQLDRLILARQRFAHGIQTLFFDHPNCI
CCCCEECCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHEECCCCEE
AFSRSGTEENPGCVVVLSNGDDGEKTLLLGDNYANKTWRDFLGNRSEHVVTNDQGEATFF
EEECCCCCCCCCEEEEEECCCCCCEEEEEECCCCCHHHHHHHCCCCCEEEECCCCCEEEE
CNAGSVSVWVIEDV
EECCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1400215; 11677609; 1527488; 8371104 [H]